(Circular) (Six-base) MAPSORT of: pFN21AB7974.seq Check: 3578 from: 1 to: 5944 pFN21AB7974 5944 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3754 278 Size: 53 83 186 936 2218 2468 Fragments arranged by size: 2468 2218 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3287 1937 Size: 1350 4594 Acc65I G'GTAC_C Cuts at: 3265 3265 Size: 5944 AclI AA'CG_TT Cuts at: 1563 4071 4444 1563 Size: 2508 373 3063 Fragments arranged by size: 3063 2508 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2573 2661 4007 5391 5659 1632 Size: 941 88 1346 1384 268 1917 Fragments arranged by size: 1917 1384 1346 941 268 88 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5747 AflIII A'CryG_T Cuts at: 1203 4843 5820 1203 Size: 3640 977 1327 Fragments arranged by size: 3640 1327 977 AgeI A'CCGG_T Cuts at: 4775 4775 Size: 5944 AhdI GACnn_n'nnGTC Cuts at: 4673 4673 Size: 5944 AleI CACnn'nnGTG Cuts at: 1166 3085 1166 Size: 1919 4025 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2925 1184 Size: 1741 4203 AlwNI CAG_nnn'CTG Cuts at: 2801 3687 5259 2801 Size: 886 1572 3486 Fragments arranged by size: 3486 1572 886 ApaI G_GGCC'C Cuts at: 2482 2876 2482 Size: 394 5550 ApaLI G'TGCA_C Cuts at: 4001 5157 4001 Size: 1156 4788 ApoI r'AATT_y Cuts at: 1493 3253 3479 3631 5740 1493 Size: 1760 226 152 2109 1697 Fragments arranged by size: 2109 1760 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4498 160 Size: 872 3466 1606 Fragments arranged by size: 3466 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5944 AvaI C'yCGr_G Cuts at: 1870 1943 2038 2274 3163 3269 1870 Size: 73 95 236 889 106 4545 Fragments arranged by size: 4545 889 236 106 95 73 BamHI G'GATC_C Cuts at: 3274 3641 3274 Size: 367 5577 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2026 2489 2503 3265 4720 Size: 325 217 627 239 463 14 762 1455 Cuts at: 4720 618 Size: 1842 Fragments arranged by size: 1842 1455 762 627 463 325 239 217 14 BanII G_rGCy'C Cuts at: 1391 2304 2482 2609 2660 2856 2876 3015 3098 Size: 913 178 127 51 196 20 139 83 Cuts at: 3098 3263 1391 Size: 165 4072 Fragments arranged by size: 4072 913 196 178 165 139 127 83 51 20 BbeI G_GCGC'C Cuts at: 2030 2030 Size: 5944 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2898 928 Size: 971 999 3974 Fragments arranged by size: 3974 999 971 BbvCI CC'TCA_GC Cuts at: 2739 2739 Size: 5944 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4135 4169 1903 Size: 34 2198 34 3678 Fragments arranged by size: 3678 2198 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3836 5046 3836 Size: 1210 4734 BclI T'GATC_A Cuts at: 1548 1548 Size: 5944 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2477 4555 136 Size: 107 122 71 631 605 805 2078 1525 Fragments arranged by size: 2078 1525 805 631 605 122 107 71 BglII A'GATC_T Cuts at: 5939 5939 Size: 5944 BlpI GC'TnA_GC Cuts at: 3361 3361 Size: 5944 Bme1580I G_kGCm'C Cuts at: 1790 2045 2482 2508 2876 4005 5161 1790 Size: 255 437 26 368 1129 1156 2573 Fragments arranged by size: 2573 1156 1129 437 368 255 26 BmgBI CAC'GTC Cuts at: 1318 2398 2726 1318 Size: 1080 328 4536 Fragments arranged by size: 4536 1080 328 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2701 2879 3675 Size: 416 53 83 399 24 1284 178 796 Cuts at: 3675 4633 442 Size: 958 1753 Fragments arranged by size: 1753 1284 958 796 416 399 178 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5944 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2480 2480 Size: 5944 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2475 2519 2636 3128 4604 1239 Size: 1236 44 117 492 1476 2579 Fragments arranged by size: 2579 1476 1236 492 117 44 Bpu10I CC'TnA_GC Cuts at: 2739 2739 Size: 5944 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2117 2288 4069 4934 5232 2117 Size: 171 1781 865 298 2829 Fragments arranged by size: 2829 1781 865 298 171 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2525 3162 4607 882 Size: 617 1026 637 1445 2219 Fragments arranged by size: 2219 1445 1026 637 617 BsaAI yAC'GTr Cuts at: 493 1204 3735 493 Size: 711 2531 2702 Fragments arranged by size: 2702 2531 711 BsaBI GATnn'nnATC Cuts at: 5938 5938 Size: 5944 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2027 3751 4133 4768 Size: 53 83 186 936 494 1724 382 635 Cuts at: 4768 275 Size: 1451 Fragments arranged by size: 1724 1451 936 635 494 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2379 2984 4377 4775 5049 5196 5607 2379 Size: 605 1393 398 274 147 411 2716 Fragments arranged by size: 2716 1393 605 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3104 3134 1178 Size: 30 165 30 1701 30 3988 Fragments arranged by size: 3988 1701 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2333 3125 1186 Size: 1147 792 4005 Fragments arranged by size: 4005 1147 792 BseYI C'CCAG_C Cuts at: 2794 5147 5643 2794 Size: 2353 496 3095 Fragments arranged by size: 3095 2353 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2387 2420 2790 1163 Size: 573 651 33 370 4317 Fragments arranged by size: 4317 651 573 370 33 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3394 4155 5183 1540 Size: 275 186 1393 761 1028 2301 Fragments arranged by size: 2301 1393 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2304 2609 2856 3098 4005 4090 5161 2304 Size: 305 247 242 907 85 1071 3087 Fragments arranged by size: 3087 1071 907 305 247 242 85 BsmI GAATG_Cn' Cuts at: 3455 3548 3455 Size: 93 5851 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2304 2482 2508 2609 2660 2856 Size: 399 255 259 178 26 101 51 196 Cuts at: 2856 2876 3015 3098 3263 4005 4090 5161 1391 Size: 20 139 83 165 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 259 255 196 178 165 139 101 85 83 51 26 20 BspEI T'CCGG_A Cuts at: 2379 2984 5607 2379 Size: 605 2623 2716 Fragments arranged by size: 2716 2623 605 BspHI T'CATG_A Cuts at: 2428 3833 5563 2428 Size: 1405 1730 2809 Fragments arranged by size: 2809 1730 1405 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3299 844 Size: 2455 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3831 1394 Size: 429 2008 3507 Fragments arranged by size: 3507 2008 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4439 4613 65 Size: 1183 3191 174 1396 Fragments arranged by size: 3191 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2052 4588 4775 1700 Size: 110 242 2536 187 2869 Fragments arranged by size: 2869 2536 242 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5944 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5944 BssSI C'ACGA_G Cuts at: 3081 4004 5016 3081 Size: 923 1012 4009 Fragments arranged by size: 4009 1012 923 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5944 BstBI TT'CG_AA Cuts at: 4748 4748 Size: 5944 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5944 BstXI CCAn_nnnn'nTGG Cuts at: 2197 2197 Size: 5944 BstYI r'GATC_y Cuts at: 1972 3274 3641 4026 4043 5495 5581 5593 5939 Size: 1302 367 385 17 1452 86 12 346 Cuts at: 5939 1972 Size: 1977 Fragments arranged by size: 1977 1452 1302 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4453 Fragments arranged by size: 4453 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5205 Fragments arranged by size: 5205 683 56 BtsI GCAGTG_nn' Cuts at: 2751 2773 2897 3460 4254 4274 2751 Size: 22 124 563 794 20 4421 Fragments arranged by size: 4421 794 563 124 22 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5944 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2827 2862 529 Size: 35 2263 35 3611 Fragments arranged by size: 3611 2263 35 35 DraI TTT'AAA Cuts at: 3248 3248 Size: 5944 DraIII CAC_nnn'GTG Cuts at: 2359 2359 Size: 5944 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4951 809 Size: 507 3635 1802 Fragments arranged by size: 3635 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2469 2894 3391 Size: 54 1425 50 275 18 639 425 497 Cuts at: 3391 4280 5909 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 639 497 425 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3391 1537 Size: 275 1579 4090 Fragments arranged by size: 4090 1579 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2549 3874 1353 Size: 1196 1325 3423 Fragments arranged by size: 3423 1325 1196 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 2188 4531 4905 5051 387 Size: 1064 138 435 164 2343 374 146 1280 Fragments arranged by size: 2343 1280 1064 435 374 164 146 138 EcoICRI GAG'CTC Cuts at: 2302 2607 2854 3096 2302 Size: 305 247 242 5150 Fragments arranged by size: 5150 305 247 242 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2475 2519 2573 2636 2661 3128 4007 Size: 393 843 44 54 63 25 467 879 Cuts at: 4007 4604 5391 5659 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 879 843 787 597 467 393 268 63 54 44 25 EcoNI CCTnn'n_nnAGG Cuts at: 2102 2102 Size: 5944 EcoO109I rG'GnC_Cy Cuts at: 2258 2479 2258 Size: 221 5723 EcoRI G'AATT_C Cuts at: 3253 3631 3253 Size: 378 5566 FspI TGC'GCA Cuts at: 4450 4450 Size: 5944 HaeII r_GCGC'y Cuts at: 1678 2030 5091 1678 Size: 352 3061 2531 Fragments arranged by size: 3061 2531 352 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2210 2242 3104 3136 4585 Size: 32 135 32 638 32 862 32 1449 Cuts at: 4585 4617 4659 4691 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1449 862 638 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3288 3534 669 Size: 988 281 1350 246 3079 Fragments arranged by size: 3079 1350 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5944 HpaI GTT'AAC Cuts at: 3534 3534 Size: 5944 KasI G'GCGC_C Cuts at: 2026 2026 Size: 5944 KpnI G_GTAC'C Cuts at: 3269 3269 Size: 5944 MfeI C'AATT_G Cuts at: 3543 3543 Size: 5944 MluI A'CGCG_T Cuts at: 5820 5820 Size: 5944 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5058 5242 1305 Size: 3753 184 2007 Fragments arranged by size: 3753 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2471 10 Size: 54 1425 343 639 3483 Fragments arranged by size: 3483 1425 639 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3085 3902 4261 4420 518 Size: 648 1919 817 359 159 2042 Fragments arranged by size: 2042 1919 817 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2144 2332 2409 2801 3360 4037 5185 5430 Size: 538 188 77 392 559 677 1148 245 Cuts at: 5430 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 559 538 392 245 188 77 NaeI GCC'GGC Cuts at: 2054 2054 Size: 5944 NarI GG'CG_CC Cuts at: 2027 2027 Size: 5944 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4453 Fragments arranged by size: 4453 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5944 NgoMIV G'CCGG_C Cuts at: 2052 2052 Size: 5944 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5944 NotI GC'GGCC_GC Cuts at: 3391 3391 Size: 5944 NspI r_CATG'y Cuts at: 1132 1229 3302 4847 1132 Size: 97 2073 1545 2229 Fragments arranged by size: 2229 2073 1545 97 PasI CC'CwG_GG Cuts at: 1348 2825 1348 Size: 1477 4467 PciI A'CATG_T Cuts at: 4843 4843 Size: 5944 PflMI CCAn_nnn'nTGG Cuts at: 1108 2785 1108 Size: 1677 4267 PmeI GTTT'AAAC Cuts at: 3248 3248 Size: 5944 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4013 4045 5526 5558 4013 Size: 32 1481 32 4399 Fragments arranged by size: 4399 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5944 PsiI TTA'TAA Cuts at: 3514 3514 Size: 5944 PspOMI G'GGCC_C Cuts at: 2478 2872 2478 Size: 394 5550 PstI C_TGCA'G Cuts at: 830 2106 2328 2444 2904 3296 830 Size: 1276 222 116 460 392 3478 Fragments arranged by size: 3478 1276 460 392 222 116 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5944 PvuII CAG'CTG Cuts at: 2144 2409 2801 2144 Size: 265 392 5287 Fragments arranged by size: 5287 392 265 SacI G_AGCT'C Cuts at: 2304 2609 2856 3098 2304 Size: 305 247 242 5150 Fragments arranged by size: 5150 305 247 242 SalI G'TCGA_C Cuts at: 1936 3286 1936 Size: 1350 4594 SbfI CC_TGCA'GG Cuts at: 3296 3296 Size: 5944 ScaI AGT'ACT Cuts at: 4192 5904 4192 Size: 1712 4232 SfcI C'TryA_G Cuts at: 826 1045 2102 2324 2440 2900 3292 4427 5108 Size: 219 1057 222 116 460 392 1135 681 Cuts at: 5108 5299 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1057 681 460 392 222 219 191 116 SfiI GGCCn_nnn'nGGCC Cuts at: 2477 2477 Size: 5944 SfoI GGC'GCC Cuts at: 2028 2028 Size: 5944 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5944 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5944 SmaI CCC'GGG Cuts at: 1872 2040 2276 3165 3271 1872 Size: 168 236 889 106 4545 Fragments arranged by size: 4545 889 236 168 106 SmlI C'TyrA_G Cuts at: 820 1017 1943 2132 2303 4048 4949 5211 820 Size: 197 926 189 171 1745 901 262 1553 Fragments arranged by size: 1745 1553 926 901 262 197 189 171 SnaBI TAC'GTA Cuts at: 493 3735 493 Size: 3242 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5944 SphI G_CATG'C Cuts at: 1132 3302 1132 Size: 2170 3774 SspI AAT'ATT Cuts at: 5 52 3868 5 Size: 47 3816 2081 Fragments arranged by size: 3816 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5944 StyI C'CwwG_G Cuts at: 513 1065 2004 2112 3383 513 Size: 552 939 108 1271 3074 Fragments arranged by size: 3074 1271 939 552 108 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2353 3971 3988 4141 4326 4771 325 Size: 1255 773 1618 17 153 185 445 1498 Fragments arranged by size: 1618 1498 1255 773 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4190 5902 96 Size: 274 80 33 51 1443 2213 1712 138 Fragments arranged by size: 2213 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5904 5936 5904 Size: 32 5912 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5944 XbaI T'CTAG_A Cuts at: 3280 3280 Size: 5944 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2786 3037 2786 Size: 251 5693 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5944 XmaI C'CCGG_G Cuts at: 1870 2038 2274 3163 3269 1870 Size: 168 236 889 106 4545 Fragments arranged by size: 4545 889 236 168 106 XmnI GAAnn'nnTTC Cuts at: 4073 4073 Size: 5944 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3752 276 Size: 53 83 186 936 2218 2468 Fragments arranged by size: 2468 2218 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SrfI SwaI