################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AclI AcsI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BalI BamHI BbeI BbsI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV FbaI FseI FspI HindIII HpaI KasI KspI MaeI MamI MfeI MluI MluNI MscI MseI MunI NarI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SbfI ScaI SfaNI SfiI SfoI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 45 GACGTC GACGT^C AccI 292 GTCGAC GT^[AC][GT]AC AccIII 770 TCCGGA T^CCGGA AcyI 45 GACGTC G[GA]^CG[CT]C AocI 339 CCTCAGG CC^T[ACGT]AGG ApaI 578 GGGCCC GGGCC^C AspHI 547 GTGCTC G[AT]GC[AT]^C AvaI 129 CCCGGG C^[CT]CG[GA]G BbrPI 960 CACGTG CAC^GTG BsaHI 45 GACGTC G[GA]^CG[CT]C BsaWI 770 TCCGGA [AT]^CCGG[AT] BseAI 770 TCCGGA T^CCGGA BsiHKAI 547 GTGCTC G[AT]GC[AT]^C BsoBI 129 CCCGGG C^[CT]CG[GA]G BspEI 770 TCCGGA T^CCGGA BsrBI 303 CCGCTC CCGCTC(-3/-3) BsrDI 426 CATTGC GCAATG(2/0) BssHII 25 GCGCGC G^CGCGC BstYI 1055 GGATCT [GA]^GATC[CT] Bsu36I 339 CCTCAGG CC^T[ACGT]AGG DrdI 160 GACCAGAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1005 GAAGAG CTCTTC(1/4) Eco81I 339 CCTCAGG CC^T[ACGT]AGG EcoT22I 562 ATGCAT ATGCA^T Hin1I 45 GACGTC G[GA]^CG[CT]C HincII 292 GTCGAC GT[CT]^[GA]AC HindII 292 GTCGAC GT[CT]^[GA]AC HinfI 501 GAGTC G^A[ACGT]TC Ksp632I 1005 GAAGAG CTCTTC(1/4) MboII 1005 GAAGA GAAGA(8/7) MflI 1055 GGATCT [GA]^GATC[CT] MroI 770 TCCGGA T^CCGGA MslI 406 CACATCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NsiI 562 ATGCAT ATGCA^T NspI 755 GCATGT [GA]CATG^[CT] PleI 501 GAGTC GAGTC(4/5) PmaCI 960 CACGTG CAC^GTG PmlI 960 CACGTG CAC^GTG PstI 101 CTGCAG CTGCA^G SalI 292 GTCGAC G^TCGAC SapI 1005 GAAGAGC GCTCTTC(1/4) SexAI 968 ACCTGGT A^CC[AT]GGT SgrAI 63 CGCCGGTG C[GA]^CCGG[CT]G SmaI 129 CCCGGG CCC^GGG XhoII 1055 GGATCT [GA]^GATC[CT] XmaCI 129 CCCGGG C^CCGGG XmaI 129 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Acc65I 572 GGTACC G^GTACC 965 GGTACC AlwI 151 GATCC GGATC(4/5) 1055 GGATC Asp718I 572 GGTACC G^GTACC 965 GGTACC BanII 578 GGGCCC G[GA]GC[CT]^C 934 GAGCCC BbvCI 1134 CCTCAGC CCTCAGC(-5/-2) 93 GCTGAGG BglI 127 GCCCCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 677 GCCTGCCTGGC BpmI 550 CTCCAG CTGGAG(16/14) 929 CTGGAG BsaAI 661 TACGTG [CT]AC^GT[GA] 960 CACGTG BsaI 262 GAGACC GGTCTC(1/5) 1035 GGTCTC BseRI 625 CTCCTC GAGGAG(10/8) 1038 CTCCTC BsgI 717 CTGCAC GTGCAG(16/14) 992 GTGCAG BsmAI 262 GAGAC GTCTC(1/5) 1036 GTCTC BspMI 103 GCAGGT ACCTGC(4/8) 569 GCAGGT HaeII 35 AGCGCC [GA]GCGC^[CT] 135 GGCGCT HgaI 522 GACGC GACGC(5/10) 167 GCGTC HphI 981 GGTGA GGTGA(8/7) 839 TCACC KpnI 572 GGTACC GGTAC^C 965 GGTACC MaeIII 724 GTGAC ^GT[ACGT]AC 818 GTGAC MspA1I 583 CAGCGG C[AC]G^C[GT]G 714 CCGCTG NaeI 203 GCCGGC GCC^GGC 415 GCCGGC NcoI 257 CCATGG C^CATGG 950 CCATGG NgoMIV 203 GCCGGC G^CCGGC 415 GCCGGC SfcI 101 CTGCAG C^T[GA][CT]AG 1030 CTACAG TaqI 250 TCGA T^CGA 293 TCGA Tsp45I 724 GTGAC ^GT[GC]AC 818 GTGAC TthHB8I 250 TCGA T^CGA 293 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI AvaII BanI BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MaeII MboI MnlI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PpuMI RsaI Sau3AI Sau96I ScrFI SmlI StyI TseI Tsp509I TspRI