(Linear) (Six-base) MAPSORT of: KIEE6354.seq Check: 8139 from: 1 to: 1155 KIEE6354.orf With 182 enzymes: SgfI * March 16, 2015 11:30 .. AatII G_ACGT'C Cuts at: 0 49 1155 Size: 49 1106 AccI GT'mk_AC Cuts at: 0 293 1155 Size: 293 862 Acc65I G'GTAC_C Cuts at: 0 572 965 1155 Size: 572 393 190 Fragments arranged by size: 572 393 190 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 302 1155 Size: 302 853 AleI CACnn'nnGTG Cuts at: 0 410 1155 Size: 410 745 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 679 1155 Size: 679 476 AlwNI CAG_nnn'CTG Cuts at: 0 243 280 1083 1155 Size: 243 37 803 72 Fragments arranged by size: 803 243 72 37 ApaI G_GGCC'C Cuts at: 0 582 1155 Size: 582 573 AvaI C'yCGr_G Cuts at: 0 129 1155 Size: 129 1026 BanI G'GyrC_C Cuts at: 0 11 67 418 572 965 1155 Size: 11 56 351 154 393 190 Fragments arranged by size: 393 351 190 154 56 11 BanII G_rGCy'C Cuts at: 0 582 938 1155 Size: 582 356 217 Fragments arranged by size: 582 356 217 BbvCI CC'TCA_GC Cuts at: 0 94 1135 1155 Size: 94 1041 20 Fragments arranged by size: 1041 94 20 BfrBI ATG'CAT Cuts at: 0 564 1155 Size: 564 591 BglI GCCn_nnn'nGGC Cuts at: 0 133 683 1155 Size: 133 550 472 Fragments arranged by size: 550 472 133 Bme1580I G_kGCm'C Cuts at: 0 72 582 961 1155 Size: 72 510 379 194 Fragments arranged by size: 510 379 194 72 BmrI ACTGGGnnnn_n' Cuts at: 0 268 429 1155 Size: 268 161 726 Fragments arranged by size: 726 268 161 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 535 950 1155 Size: 535 415 205 Fragments arranged by size: 535 415 205 Bpu10I CC'TnA_GC Cuts at: 0 94 1135 1155 Size: 94 1041 20 Fragments arranged by size: 1041 94 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 613 619 631 1155 Size: 613 6 12 524 Fragments arranged by size: 613 524 12 6 BsaI GGTCTCn'nnnn_ Cuts at: 0 256 1041 1155 Size: 256 785 114 Fragments arranged by size: 785 256 114 BsaAI yAC'GTr Cuts at: 0 663 962 1155 Size: 663 299 193 Fragments arranged by size: 663 299 193 BsaHI Gr'CG_yC Cuts at: 0 46 1155 Size: 46 1109 BsaWI w'CCGG_w Cuts at: 0 770 1155 Size: 770 385 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 946 976 1155 Size: 946 30 179 Fragments arranged by size: 946 179 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 616 1029 1155 Size: 616 413 126 Fragments arranged by size: 616 413 126 BseYI C'CCAG_C Cuts at: 0 110 581 731 986 1155 Size: 110 471 150 255 169 Fragments arranged by size: 471 255 169 150 110 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 702 1013 1155 Size: 702 311 142 Fragments arranged by size: 702 311 142 BsiHKAI G_wGCw'C Cuts at: 0 551 1155 Size: 551 604 Bsp1286I G_dGCh'C Cuts at: 0 72 551 582 938 961 1155 Size: 72 479 31 356 23 194 Fragments arranged by size: 479 356 194 72 31 23 BspEI T'CCGG_A Cuts at: 0 770 1155 Size: 770 385 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 94 560 1155 Size: 94 466 595 Fragments arranged by size: 595 466 94 BsrBI CCG'CTC Cuts at: 0 305 1155 Size: 305 850 BsrDI GCAATG_nn' Cuts at: 0 425 1155 Size: 425 730 BsrFI r'CCGG_y Cuts at: 0 64 203 415 1155 Size: 64 139 212 740 Fragments arranged by size: 740 212 139 64 BssHII G'CGCG_C Cuts at: 0 25 1155 Size: 25 1130 BstYI r'GATC_y Cuts at: 0 1055 1155 Size: 1055 100 Bsu36I CC'TnA_GG Cuts at: 0 340 1155 Size: 340 815 BtgI C'CryG_G Cuts at: 0 257 356 505 950 1155 Size: 257 99 149 445 205 Fragments arranged by size: 445 257 205 149 99 BtsI GCAGTG_nn' Cuts at: 0 143 241 456 1126 1155 Size: 143 98 215 670 29 Fragments arranged by size: 670 215 143 98 29 DrdI GACnn_nn'nnGTC Cuts at: 0 166 1155 Size: 166 989 EarI CTCTTCn'nnn_ Cuts at: 0 1000 1155 Size: 1000 155 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 302 535 950 1155 Size: 302 233 415 205 Fragments arranged by size: 415 302 233 205 EcoO109I rG'GnC_Cy Cuts at: 0 330 345 1014 1080 1106 1155 Size: 330 15 669 66 26 49 Fragments arranged by size: 669 330 66 49 26 15 HaeII r_GCGC'y Cuts at: 0 39 139 1155 Size: 39 100 1016 Fragments arranged by size: 1016 100 39 HincII GTy'rAC Cuts at: 0 294 1155 Size: 294 861 KpnI G_GTAC'C Cuts at: 0 576 969 1155 Size: 576 393 186 Fragments arranged by size: 576 393 186 MslI CAynn'nnrTG Cuts at: 0 410 1155 Size: 410 745 MspA1I CmG'CkG Cuts at: 0 585 716 1155 Size: 585 131 439 Fragments arranged by size: 585 439 131 NaeI GCC'GGC Cuts at: 0 205 417 1155 Size: 205 212 738 Fragments arranged by size: 738 212 205 NcoI C'CATG_G Cuts at: 0 257 950 1155 Size: 257 693 205 Fragments arranged by size: 693 257 205 NgoMIV G'CCGG_C Cuts at: 0 203 415 1155 Size: 203 212 740 Fragments arranged by size: 740 212 203 NsiI A_TGCA'T Cuts at: 0 566 1155 Size: 566 589 NspI r_CATG'y Cuts at: 0 759 1155 Size: 759 396 PfoI T'CCnGG_A Cuts at: 0 1102 1155 Size: 1102 53 PmlI CAC'GTG Cuts at: 0 962 1155 Size: 962 193 PpuMI rG'GwC_Cy Cuts at: 0 330 345 1106 1155 Size: 330 15 761 49 Fragments arranged by size: 761 330 49 15 PspOMI G'GGCC_C Cuts at: 0 578 1155 Size: 578 577 PstI C_TGCA'G Cuts at: 0 105 1155 Size: 105 1050 SalI G'TCGA_C Cuts at: 0 292 1155 Size: 292 863 SanDI GG'GwC_CC Cuts at: 0 345 1155 Size: 345 810 SapI GCTCTTCn'nnn_ Cuts at: 0 1000 1155 Size: 1000 155 SexAI A'CCwGG_T Cuts at: 0 968 1155 Size: 968 187 SfcI C'TryA_G Cuts at: 0 101 1030 1155 Size: 101 929 125 Fragments arranged by size: 929 125 101 SgrAI Cr'CCGG_yG Cuts at: 0 64 1155 Size: 64 1091 SmaI CCC'GGG Cuts at: 0 131 1155 Size: 131 1024 SmlI C'TyrA_G Cuts at: 0 628 634 646 1155 Size: 628 6 12 509 Fragments arranged by size: 628 509 12 6 StyI C'CwwG_G Cuts at: 0 182 257 743 938 950 1155 Size: 182 75 486 195 12 205 Fragments arranged by size: 486 205 195 182 75 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 250 429 588 913 1035 1155 Size: 250 179 159 325 122 120 Fragments arranged by size: 325 250 179 159 122 120 TatI w'GTAC_w Cuts at: 0 650 1155 Size: 650 505 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 135 167 946 978 1155 Size: 135 32 779 32 177 Fragments arranged by size: 779 177 135 32 32 XmaI C'CCGG_G Cuts at: 0 129 1155 Size: 129 1026 ZraI GAC'GTC Cuts at: 0 47 1155 Size: 47 1108 Enzymes that do cut: AatII AccI Acc65I AcuI AleI AlfI AlwNI ApaI AvaI BanI BanII BbvCI BfrBI BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII BstYI Bsu36I BtgI BtsI DrdI EarI Eco57MI EcoO109I HaeII HincII KpnI MslI MspA1I NaeI NcoI NgoMIV NsiI NspI PfoI PmlI PpuMI PspOMI PstI SalI SanDI SapI SexAI SfcI SgrAI SmaI SmlI StyI TaqII TatI TstI XmaI ZraI Enzymes that do not cut: AarI AclI AfeI AflII AflIII AgeI AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BcgI BciVI BclI BglII BlpI BmgBI BmtI BplI BsaBI BsiEI BsiWI BsmI BsmBI BspHI BsrGI BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII EaeI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI KasI MfeI MluI MmeI MscI NarI NdeI NheI NotI NruI PacI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII SbfI ScaI SfiI SfoI SgfI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmnI