################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AspEI AspI AviII AvrII BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaHI BsaWI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspMI BsrBI BsrGI BssHII BssSI BstAPI BstEII BstPI BstUI Bsu36I CelII CfoI ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KspI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NgoMIV NotI NruI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmlI SnaBI SpeI Sse8387I SspBI Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### Acc65I 1058 GGTACC G^GTACC AccI 392 GTATAC GT^[AC][GT]AC Alw44I 689 GTGCAC G^TGCAC ApaLI 689 GTGCAC G^TGCAC AseI 961 ATTAAT AT^TAAT AsnI 961 ATTAAT AT^TAAT Asp700I 1511 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1058 GGTACC G^GTACC AspHI 689 GTGCAC G[AT]GC[AT]^C BalI 1237 TGGCCA TGG^CCA BanI 1058 GGTACC G^G[CT][GA]CC BbvI 104 GCAGC GCAGC(8/12) BmyI 689 GTGCAC G[AGT]GC[ACT]^C BsaAI 893 TACGTG [CT]AC^GT[GA] BsaBI 463 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 689 GTGCAC G[AT]GC[AT]^C BsmBI 149 CGTCTC CGTCTC(1/5) BsmI 162 GCATTC GAATGC(1/-1) Bsp1286I 689 GTGCAC G[AGT]GC[ACT]^C BsrFI 1061 ACCGGC [GA]^CCGG[CT] Bst1107I 392 GTATAC GTA^TAC BstF5I 834 GGATG GGATG(2/0) BstXI 330 CCAAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 392 GTATAC GTA^TAC Cfr10I 1061 ACCGGC [GA]^CCGG[CT] DraII 1496 AGGACCC [GA]G^G[ACGT]CC[CT] DraIII 218 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1178 CCGTGG C^C[GA][CT]GG EarI 1393 GAAGAG CTCTTC(1/4) EcoO109I 1496 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRV 467 GATATC GAT^ATC EcoT14I 240 CCTTGG C^C[AT][AT]GG EcoT22I 160 ATGCAT ATGCA^T Fnu4HI 104 GCAGC GC^[ACGT]GC FokI 834 GGATG GGATG(9/13) ItaI 104 GCAGC GC^[ACGT]GC KpnI 1058 GGTACC GGTAC^C Ksp632I 1393 GAAGAG CTCTTC(1/4) MamI 463 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1237 TGGCCA TGG^CCA MscI 1237 TGGCCA TGG^CCA MspA1I 1388 CAGCTG C[AC]G^C[GT]G NdeI 605 CATATG CA^TATG NheI 984 GCTAGC G^CTAGC NsiI 160 ATGCAT ATGCA^T PpuMI 1496 AGGACCC [GA]G^G[AT]CC[CT] PshBI 961 ATTAAT AT^TAAT PstI 443 CTGCAG CTGCA^G PvuII 1388 CAGCTG CAG^CTG ScaI 1094 AGTACT AGT^ACT SfaNI 930 GCATC GCATC(5/9) SmaI 1500 CCCGGG CCC^GGG SphI 1559 GCATGC GCATG^C SspI 1464 AATATT AAT^ATT StuI 139 AGGCCT AGG^CCT StyI 240 CCTTGG C^C[AT][AT]GG SwaI 419 ATTTAAAT ATTT^AAAT TseI 104 GCAGC G^C[AT]GC XcmI 885 CCAAATTCTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1500 CCCGGG C^CCGGG XmaI 1500 CCCGGG C^CCGGG XmnI 1511 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AciI 210 GCGG CCGC(-3/-1) 429 GCGG AflIII 1112 ACATGT A^C[GA][CT]GT 1407 ACATGT AvaI 1004 CCCGAG C^[CT]CG[GA]G 1500 CCCGGG AvaII 1429 GGACC G^G[AT]CC 1497 GGACC BbsI 1154 GAAGAC GAAGAC(2/6) 1550 GAAGAC BcnI 1500 CCCGG CC^[GC]GG 1501 CCGGG BpuAI 1154 GAAGAC GAAGAC(2/6) 1550 GAAGAC BsaI 213 GAGACC GGTCTC(1/5) 524 GGTCTC BseRI 955 CTCCTC GAGGAG(10/8) 1258 GAGGAG BsgI 696 CTGCAC GTGCAG(16/14) 1192 CTGCAC BsmFI 340 GGGAC GGGAC(10/14) 1428 GGGAC BsoBI 1004 CCCGAG C^[CT]CG[GA]G 1500 CCCGGG BspHI 291 TCATGA T^CATGA 851 TCATGA BspLU11I 1112 ACATGT A^CATGT 1407 ACATGT BsrDI 206 CATTGC GCAATG(2/0) 256 GCAATG BsrI 177 CCAGT ACTGG(1/-1) 360 CCAGT BstBI 1418 TTCGAA TT^CGAA 1508 TTCGAA Cfr13I 1429 GGACC G^G[ACGT]CC 1497 GGACC DraI 420 TTTAAA TTT^AAA 732 TTTAAA EaeI 1063 CGGCCA [CT]^GGCC[GA] 1237 TGGCCA HapII 1062 CCGG C^CGG 1501 CCGG HindIII 332 AAGCTT A^AGCTT 589 AAGCTT HpaII 1062 CCGG C^CGG 1501 CCGG HphI 319 GGTGA GGTGA(8/7) 364 TCACC MslI 252 CATGGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 852 CATGAGTATG MspI 1062 CCGG C^CGG 1501 CCGG MwoI 201 GCCCCCATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1390 GCTGAAGAGGC NciI 1500 CCCGG CC^[GC]GG 1501 CCGGG NspV 1418 TTCGAA TT^CGAA 1508 TTCGAA PleI 1327 GACTC GAGTC(4/5) 516 GAGTC RcaI 291 TCATGA T^CATGA 851 TCATGA Sau96I 1429 GGACC G^G[ACGT]CC 1497 GGACC SfcI 368 CTACAG C^T[GA][CT]AG 443 CTGCAG SfuI 1418 TTCGAA TT^CGAA 1508 TTCGAA TspRI 222 CAGTG CA[GC]TG[ACGT][ACGT]^ 437 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI BfaI BsaJI BsiYI BslI BsmAI BssKI BstNI BstYI Cac8I DdeI DpnII EcoRII HaeIII HinfI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MvaI NdeII NlaIII NlaIV NspI RsaI Sau3AI ScrFI TaqI TfiI Tru9I Tsp45I Tsp509I TthHB8I XhoII