(Circular) (Six-base) MAPSORT of: pFN21AB9420.seq Check: 1150 from: 1 to: 6274 pFN21AB9420 6274 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4084 278 Size: 53 83 186 936 2548 2468 Fragments arranged by size: 2548 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2398 3617 1937 Size: 461 1219 4594 Fragments arranged by size: 4594 1219 461 Acc65I G'GTAC_C Cuts at: 3063 3595 3063 Size: 532 5742 AclI AA'CG_TT Cuts at: 1563 4401 4774 1563 Size: 2838 373 3063 Fragments arranged by size: 3063 2838 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3350 3357 3417 4337 5721 5989 1632 Size: 1718 7 60 920 1384 268 1917 Fragments arranged by size: 1917 1718 1384 920 268 60 7 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6077 AflIII A'CryG_T Cuts at: 1203 3117 3412 5173 6150 1203 Size: 1914 295 1761 977 1327 Fragments arranged by size: 1914 1761 1327 977 295 AgeI A'CCGG_T Cuts at: 5105 5105 Size: 6274 AhdI GACnn_n'nnGTC Cuts at: 5003 5003 Size: 6274 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6274 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6274 AlwNI CAG_nnn'CTG Cuts at: 4017 5589 4017 Size: 1572 4702 ApaLI G'TGCA_C Cuts at: 2694 4331 5487 2694 Size: 1637 1156 3481 Fragments arranged by size: 3481 1637 1156 ApoI r'AATT_y Cuts at: 1493 2012 2650 2892 3359 3517 3583 3809 3961 Size: 519 638 242 467 158 66 226 152 Cuts at: 3961 6070 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 638 519 467 242 226 158 152 66 AseI AT'TA_AT Cuts at: 160 1032 2967 4828 160 Size: 872 1935 1861 1606 Fragments arranged by size: 1935 1861 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6274 AvaI C'yCGr_G Cuts at: 1870 1943 3009 3505 3599 1870 Size: 73 1066 496 94 4545 Fragments arranged by size: 4545 1066 496 94 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3047 3080 3047 Size: 33 6241 BamHI G'GATC_C Cuts at: 3604 3971 3604 Size: 367 5907 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3063 3595 5050 618 Size: 325 217 627 1276 532 1455 1842 Fragments arranged by size: 1842 1455 1276 627 532 325 217 BanII G_rGCy'C Cuts at: 1391 3593 1391 Size: 2202 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3166 3562 928 Size: 971 1267 396 3640 Fragments arranged by size: 3640 1267 971 396 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4465 4499 1903 Size: 34 2528 34 3678 Fragments arranged by size: 3678 2528 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4166 5376 4166 Size: 1210 5064 BclI T'GATC_A Cuts at: 1548 1548 Size: 6274 BfrBI ATG'CAT Cuts at: 2167 2167 Size: 6274 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4885 136 Size: 107 122 71 631 605 3213 1525 Fragments arranged by size: 3213 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6269 6269 Size: 6274 BlpI GC'TnA_GC Cuts at: 3691 3691 Size: 6274 Bme1580I G_kGCm'C Cuts at: 1790 2698 4335 5491 1790 Size: 908 1637 1156 2573 Fragments arranged by size: 2573 1637 1156 908 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6274 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4005 4963 442 Size: 416 53 83 399 24 2588 958 1753 Fragments arranged by size: 2588 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2993 1056 Size: 1937 4337 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4934 1239 Size: 3695 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4399 5264 5562 4399 Size: 865 298 5111 Fragments arranged by size: 5111 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2212 2535 4937 882 Size: 617 713 323 2402 2219 Fragments arranged by size: 2402 2219 713 617 323 BsaAI yAC'GTr Cuts at: 493 1204 2900 4065 493 Size: 711 1696 1165 2702 Fragments arranged by size: 2702 1696 1165 711 BsaBI GATnn'nnATC Cuts at: 2472 6268 2472 Size: 3796 2478 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4081 4463 5098 275 Size: 53 83 186 936 2548 382 635 1451 Fragments arranged by size: 2548 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4707 5105 5379 5526 5937 4707 Size: 398 274 147 411 5044 Fragments arranged by size: 5044 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2943 2973 1178 Size: 30 165 30 1540 30 4479 Fragments arranged by size: 4479 1540 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2951 3278 1186 Size: 1765 327 4182 Fragments arranged by size: 4182 1765 327 BseYI C'CCAG_C Cuts at: 5477 5973 5477 Size: 496 5778 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2686 3182 1163 Size: 573 950 496 4255 Fragments arranged by size: 4255 950 573 496 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3724 4485 5513 1540 Size: 275 186 1723 761 1028 2301 Fragments arranged by size: 2301 1723 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2698 4335 4420 5491 2698 Size: 1637 85 1071 3481 Fragments arranged by size: 3481 1637 1071 85 BsmI GAATG_Cn' Cuts at: 2167 3785 3878 2167 Size: 1618 93 4563 Fragments arranged by size: 4563 1618 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2160 2160 Size: 6274 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2698 3593 4335 4420 5491 1391 Size: 399 908 895 742 85 1071 2174 Fragments arranged by size: 2174 1071 908 895 742 399 85 BspEI T'CCGG_A Cuts at: 5937 5937 Size: 6274 BspHI T'CATG_A Cuts at: 2296 2856 4163 5893 2296 Size: 560 1307 1730 2677 Fragments arranged by size: 2677 1730 1307 560 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3629 844 Size: 2785 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4161 1394 Size: 429 2338 3507 Fragments arranged by size: 3507 2338 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2210 2268 4769 4943 65 Size: 1183 962 58 2501 174 1396 Fragments arranged by size: 2501 1396 1183 962 174 58 BsrFI r'CCGG_y Cuts at: 1700 1810 3066 4918 5105 1700 Size: 110 1256 1852 187 2869 Fragments arranged by size: 2869 1852 1256 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6274 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6274 BssSI C'ACGA_G Cuts at: 4334 5346 4334 Size: 1012 5262 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6274 BstBI TT'CG_AA Cuts at: 3424 3514 5078 3424 Size: 90 1564 4620 Fragments arranged by size: 4620 1564 90 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6274 BstXI CCAn_nnnn'nTGG Cuts at: 2342 2342 Size: 6274 BstYI r'GATC_y Cuts at: 1972 2192 2409 2670 3604 3971 4356 4373 5825 Size: 220 217 261 934 367 385 17 1452 Cuts at: 5825 5911 5923 6269 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 934 385 367 346 261 220 217 86 17 12 BstZ17I GTA'TAC Cuts at: 2399 2399 Size: 6274 BtgI C'CryG_G Cuts at: 513 1065 3183 513 Size: 552 2118 3604 Fragments arranged by size: 3604 2118 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5535 Fragments arranged by size: 5535 683 56 BtsI GCAGTG_nn' Cuts at: 2441 3790 4584 4604 2441 Size: 1349 794 20 4111 Fragments arranged by size: 4111 1349 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6274 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6239 DraI TTT'AAA Cuts at: 2427 2739 3578 2427 Size: 312 839 5123 Fragments arranged by size: 5123 839 312 DraIII CAC_nnn'GTG Cuts at: 2228 2228 Size: 6274 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5281 809 Size: 507 3965 1802 Fragments arranged by size: 3965 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3068 3242 3721 Size: 54 1425 50 275 18 1238 174 479 Cuts at: 3721 4610 6239 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1238 889 479 275 174 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3721 1537 Size: 275 1909 4090 Fragments arranged by size: 4090 1909 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3393 4204 1353 Size: 2040 811 3423 Fragments arranged by size: 3423 2040 811 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4861 5235 5381 387 Size: 1064 138 3272 374 146 1280 Fragments arranged by size: 3272 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3350 3357 3417 4337 4934 5721 5989 Size: 393 1718 7 60 920 597 787 268 Cuts at: 5989 1239 Size: 1524 Fragments arranged by size: 1718 1524 920 787 597 393 268 60 7 EcoO109I rG'GnC_Cy Cuts at: 3502 3502 Size: 6274 EcoRI G'AATT_C Cuts at: 3583 3961 3583 Size: 378 5896 EcoRV GAT'ATC Cuts at: 2474 2474 Size: 6274 FspI TGC'GCA Cuts at: 4780 4780 Size: 6274 HaeII r_GCGC'y Cuts at: 1678 5421 1678 Size: 3743 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2482 2514 4915 4947 4989 Size: 32 135 32 910 32 2401 32 42 Cuts at: 4989 5021 1373 Size: 32 2626 Fragments arranged by size: 2626 2401 910 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3618 3864 669 Size: 988 281 1680 246 3079 Fragments arranged by size: 3079 1680 988 281 246 HindIII A'AGCT_T Cuts at: 748 2337 2594 748 Size: 1589 257 4428 Fragments arranged by size: 4428 1589 257 HpaI GTT'AAC Cuts at: 3864 3864 Size: 6274 KpnI G_GTAC'C Cuts at: 3067 3599 3067 Size: 532 5742 MfeI C'AATT_G Cuts at: 3873 3873 Size: 6274 MluI A'CGCG_T Cuts at: 6150 6150 Size: 6274 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2174 3235 5388 5572 1305 Size: 869 1061 2153 184 2007 Fragments arranged by size: 2153 2007 1061 869 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3244 10 Size: 54 1425 343 1412 3040 Fragments arranged by size: 3040 1425 1412 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2261 2861 4232 4591 4750 518 Size: 648 1095 600 1371 359 159 2042 Fragments arranged by size: 2042 1371 1095 648 600 359 159 MspA1I CmG'CkG Cuts at: 1606 3395 3690 4367 5515 5760 1606 Size: 1789 295 677 1148 245 2120 Fragments arranged by size: 2120 1789 1148 677 295 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5722 NdeI CA'TA_TG Cuts at: 387 2611 387 Size: 2224 4050 NheI G'CTAG_C Cuts at: 1052 2989 1052 Size: 1937 4337 NotI GC'GGCC_GC Cuts at: 3721 3721 Size: 6274 NsiI A_TGCA'T Cuts at: 2169 2169 Size: 6274 NspI r_CATG'y Cuts at: 1132 1229 2747 3121 3416 3449 3568 3632 5177 Size: 97 1518 374 295 33 119 64 1545 Cuts at: 5177 1132 Size: 2229 Fragments arranged by size: 2229 1545 1518 374 295 119 97 64 33 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6274 PciI A'CATG_T Cuts at: 3117 3412 5173 3117 Size: 295 1761 4218 Fragments arranged by size: 4218 1761 295 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6274 PfoI T'CCnGG_A Cuts at: 3172 3172 Size: 6274 PmeI GTTT'AAAC Cuts at: 3578 3578 Size: 6274 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3214 3246 4343 4375 5856 5888 3214 Size: 32 1097 32 1481 32 3600 Fragments arranged by size: 3600 1481 1097 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3502 3502 Size: 6274 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6274 PsiI TTA'TAA Cuts at: 3844 3844 Size: 6274 PstI C_TGCA'G Cuts at: 830 2452 3626 830 Size: 1622 1174 3478 Fragments arranged by size: 3478 1622 1174 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6274 PvuII CAG'CTG Cuts at: 3395 3395 Size: 6274 SalI G'TCGA_C Cuts at: 1936 3616 1936 Size: 1680 4594 SbfI CC_TGCA'GG Cuts at: 3626 3626 Size: 6274 ScaI AGT'ACT Cuts at: 3101 4522 6234 3101 Size: 1421 1712 3141 Fragments arranged by size: 3141 1712 1421 SfcI C'TryA_G Cuts at: 826 1045 2373 2448 3622 4757 5438 5629 826 Size: 219 1328 75 1174 1135 681 191 1471 Fragments arranged by size: 1471 1328 1174 1135 681 219 191 75 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6274 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6274 SmaI CCC'GGG Cuts at: 1872 3507 3601 1872 Size: 1635 94 4545 Fragments arranged by size: 4545 1635 94 SmlI C'TyrA_G Cuts at: 820 1017 1943 4378 5279 5541 820 Size: 197 926 2435 901 262 1553 Fragments arranged by size: 2435 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4065 493 Size: 3572 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6274 SphI G_CATG'C Cuts at: 1132 3568 3632 1132 Size: 2436 64 3774 Fragments arranged by size: 3774 2436 64 SspI AAT'ATT Cuts at: 5 52 3471 4198 5 Size: 47 3419 727 2081 Fragments arranged by size: 3419 2081 727 47 StuI AGG'CCT Cuts at: 1517 2146 1517 Size: 629 5645 StyI C'CwwG_G Cuts at: 513 1065 2245 3713 513 Size: 552 1180 1468 3074 Fragments arranged by size: 3074 1468 1180 552 SwaI ATTT'AAAT Cuts at: 2427 2427 Size: 6274 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3337 4301 4318 4471 4656 5101 325 Size: 1255 1757 964 17 153 185 445 1498 Fragments arranged by size: 1757 1498 1255 964 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2184 2832 3099 Size: 274 80 33 51 1443 207 648 267 Cuts at: 3099 3417 4520 6232 96 Size: 318 1103 1712 138 Fragments arranged by size: 1712 1443 1103 648 318 274 267 207 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2943 2975 3168 3200 6234 6266 2943 Size: 32 193 32 3034 32 2951 Fragments arranged by size: 3034 2951 193 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6274 XbaI T'CTAG_A Cuts at: 3610 3610 Size: 6274 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2897 2897 Size: 6274 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6274 XmaI C'CCGG_G Cuts at: 1870 3505 3599 1870 Size: 1635 94 4545 Fragments arranged by size: 4545 1635 94 XmnI GAAnn'nnTTC Cuts at: 3520 4403 3520 Size: 883 5391 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4082 276 Size: 53 83 186 936 2548 2468 Fragments arranged by size: 2548 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI Bsu36I EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI