Restriction map for
pFN21AE1387
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AhdI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AviII BbeI BbrPI BbvCI BcgI
BlpI Bpu1102I BsaAI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I CelII ClaI
CpoI DraII DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoO109I EcoRV
EcoT22I FseI FspI HaeII HindIII
HpaI KasI KpnI KspI MluI
MroI MspA1I NarI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SapI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccII 2729 CGCG CG^CG
Asp700I 582 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1827 CCTAGG C^CTAGG
BalI 2592 TGGCCA TGG^CCA
BciVI 2926 GTATCC GTATCC(6/5)
BclI 2667 TGATCA T^GATCA
BglI 1158 GCCTTGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1558 AGATCT A^GATCT
BlnI 1827 CCTAGG C^CTAGG
BsaI 2096 GAGACC GGTCTC(1/5)
BsaWI 854 TCCGGT [AT]^CCGG[AT]
BsiEI 2725 CGACCG CG[GA][CT]^CG
BspMI 2337 ACCTGC ACCTGC(4/8)
BsrFI 2528 GCCGGC [GA]^CCGG[CT]
BstUI 2729 CGCG CG^CG
BstXI 1259 CCATAATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 2730 GCGC GCG^C
Cfr10I 2528 GCCGGC [GA]^CCGG[CT]
DrdI 1138 GACAAAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 2667 TGATCA T^GATCA
HhaI 2730 GCGC GCG^C
HinP1I 2730 GCGC G^CGC
HincII 1820 GTCGAC GT[CT]^[GA]AC
HindII 1820 GTCGAC GT[CT]^[GA]AC
MfeI 656 CAATTG C^AATTG
MluNI 2592 TGGCCA TGG^CCA
MscI 2592 TGGCCA TGG^CCA
MunI 656 CAATTG C^AATTG
MvnI 2729 CGCG CG^CG
NaeI 2528 GCCGGC GCC^GGC
NdeI 794 CATATG CA^TATG
NgoMIV 2528 GCCGGC G^CCGGC
SalI 1820 GTCGAC G^TCGAC
ScaI 314 AGTACT AGT^ACT
SexAI 2625 ACCAGGT A^CC[AT]GGT
SfcI 817 CTACAG C^T[GA][CT]AG
SphI 1328 GCATGC GCATG^C
StuI 2344 AGGCCT AGG^CCT
XcmI 615 CCAGATTAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 582 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccI 1715 GTAGAC GT^[AC][GT]AC
1820 GTCGAC
AcyI 1928 GACGCC G[GA]^CG[CT]C
2391 GACGCC
AflII 1650 CTTAAG C^TTAAG
2277 CTTAAG
Alw44I 1176 GTGCAC G^TGCAC
1407 GTGCAC
AlwNI 527 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
2312 CAGCCTCTG
AocI 2537 CCTCAGG CC^T[ACGT]AGG
2773 CCTGAGG
ApaLI 1176 GTGCAC G^TGCAC
1407 GTGCAC
AspHI 1176 GTGCAC G[AT]GC[AT]^C
1407 GTGCAC
AvaI 1784 CTCGGG C^[CT]CG[GA]G
2354 CTCGGG
BamHI 1269 GGATCC G^GATCC
1444 GGATCC
BanI 1778 GGTGCC G^G[CT][GA]CC
2700 GGCACC
BbsI 141 GAAGAC GAAGAC(2/6)
1593 GTCTTC
BfrI 1650 CTTAAG C^TTAAG
2277 CTTAAG
BpuAI 141 GAAGAC GAAGAC(2/6)
1593 GTCTTC
BsaHI 1928 GACGCC G[GA]^CG[CT]C
2391 GACGCC
BsgI 2738 CTGCAC GTGCAG(16/14)
1127 GTGCAG
BsiHKAI 1176 GTGCAC G[AT]GC[AT]^C
1407 GTGCAC
BsoBI 1784 CTCGGG C^[CT]CG[GA]G
2354 CTCGGG
BspHI 603 TCATGA T^CATGA
1047 TCATGA
BsrDI 637 GCAATG GCAATG(2/0)
671 GCAATG
BstEII 1972 GGTCACC G^GT[ACGT]ACC
2621 GGTTACC
BstPI 1972 GGTCACC G^GT[ACGT]ACC
2621 GGTTACC
Bsu36I 2537 CCTCAGG CC^T[ACGT]AGG
2773 CCTGAGG
EaeI 2530 CGGCCA [CT]^GGCC[GA]
2592 TGGCCA
EarI 2839 GAAGAG CTCTTC(1/4)
2847 GAAGAG
Eco81I 2537 CCTCAGG CC^T[ACGT]AGG
2773 CCTGAGG
EcoO65I 1972 GGTCACC G^GT[ACGT]ACC
2621 GGTTACC
EcoRI 538 GAATTC G^AATTC
2157 GAATTC
FauI 1755 CCCGC CCCGC(4/6)
2611 CCCGC
HgaI 1928 GACGC GACGC(5/10)
2391 GACGC
Hin1I 1928 GACGCC G[GA]^CG[CT]C
2391 GACGCC
Ksp632I 2839 GAAGAG CTCTTC(1/4)
2847 GAAGAG
MaeII 1397 ACGT A^CGT
2934 ACGT
MslI 1260 CATAATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1329 CATGCCGATG
NcoI 577 CCATGG C^CATGG
2766 CCATGG
NspI 455 ACATGC [GA]CATG^[CT]
1328 GCATGC
RcaI 603 TCATGA T^CATGA
1047 TCATGA
SmlI 1650 CTTAAG C^T[CT][GA]AG
2277 CTTAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanII BbvI BcnI
BfaI BmyI BpmI BsaBI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DsaI EcoNI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeIII MamI MboI MboII MflI
MnlI MseI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII