(Circular) (Six-base) MAPSORT of: pFN21AE1387.seq Check: 2524 from: 1 to: 7651 pFN21AE1387 7651 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4351 4351 Size: 7651 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5461 278 Size: 53 83 186 936 3925 2468 Fragments arranged by size: 3925 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3721 3826 4994 1937 Size: 1784 105 1168 4594 Fragments arranged by size: 4594 1784 1168 105 Acc65I G'GTAC_C Cuts at: 4972 4972 Size: 7651 AclI AA'CG_TT Cuts at: 1563 5778 6151 1563 Size: 4215 373 3063 Fragments arranged by size: 4215 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2183 2437 4239 4554 5714 7098 7366 1632 Size: 551 254 1802 315 1160 1384 268 1917 Fragments arranged by size: 1917 1802 1384 1160 551 315 268 254 AflII C'TTAA_G Cuts at: 820 1017 3655 4282 820 Size: 197 2638 627 4189 Fragments arranged by size: 4189 2638 627 197 AflIII A'CryG_T Cuts at: 1203 6550 7527 1203 Size: 5347 977 1327 Fragments arranged by size: 5347 1327 977 AgeI A'CCGG_T Cuts at: 6482 6482 Size: 7651 AhdI GACnn_n'nnGTC Cuts at: 6380 6380 Size: 7651 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7651 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3356 1184 Size: 2172 5479 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4231 4263 4231 Size: 32 7619 AlwNI CAG_nnn'CTG Cuts at: 2537 4322 5394 6966 2537 Size: 1785 1072 1572 3222 Fragments arranged by size: 3222 1785 1572 1072 ApaLI G'TGCA_C Cuts at: 3181 3412 5708 6864 3181 Size: 231 2296 1156 3968 Fragments arranged by size: 3968 2296 1156 231 ApoI r'AATT_y Cuts at: 1493 2067 2543 2699 2755 4078 4162 4752 4910 Size: 574 476 156 56 1323 84 590 158 Cuts at: 4910 4960 5186 5338 7447 1493 Size: 50 226 152 2109 1697 Fragments arranged by size: 2109 1697 1323 590 574 476 226 158 156 152 84 56 50 AseI AT'TA_AT Cuts at: 160 1032 6205 160 Size: 872 5173 1606 Fragments arranged by size: 5173 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7651 AvaI C'yCGr_G Cuts at: 1870 1943 3789 4359 4976 1870 Size: 73 1846 570 617 4545 Fragments arranged by size: 4545 1846 617 570 73 AvrII C'CTAG_G Cuts at: 3832 3832 Size: 7651 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2503 2536 2562 2595 2503 Size: 33 26 33 7559 Fragments arranged by size: 7559 33 33 26 BamHI G'GATC_C Cuts at: 3274 3449 4981 5348 3274 Size: 175 1532 367 5577 Fragments arranged by size: 5577 1532 367 175 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3783 4705 4972 6427 618 Size: 325 217 627 1996 922 267 1455 1842 Fragments arranged by size: 1996 1842 1455 922 627 325 267 217 BanII G_rGCy'C Cuts at: 1391 2738 3107 3264 3845 3905 4970 1391 Size: 1347 369 157 581 60 1065 4072 Fragments arranged by size: 4072 1347 1065 581 369 157 60 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2153 3591 928 Size: 971 254 1438 4988 Fragments arranged by size: 4988 1438 971 254 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5842 5876 1903 Size: 34 3905 34 3678 Fragments arranged by size: 3905 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4942 5543 6753 4942 Size: 601 1210 5840 Fragments arranged by size: 5840 1210 601 BclI T'GATC_A Cuts at: 1548 4672 1548 Size: 3124 4527 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3169 6262 136 Size: 107 122 71 631 605 1497 3093 1525 Fragments arranged by size: 3093 1525 1497 631 605 122 107 71 BglII A'GATC_T Cuts at: 3563 7646 3563 Size: 4083 3568 BlpI GC'TnA_GC Cuts at: 5068 5068 Size: 7651 Bme1580I G_kGCm'C Cuts at: 1790 3185 3416 3788 5712 6868 1790 Size: 1395 231 372 1924 1156 2573 Fragments arranged by size: 2573 1924 1395 1156 372 231 BmgBI CAC'GTC Cuts at: 1318 4940 1318 Size: 3622 4029 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2916 3905 4100 Size: 416 53 83 399 24 1499 989 195 Cuts at: 4100 5382 6340 442 Size: 1282 958 1753 Fragments arranged by size: 1753 1499 1282 989 958 416 399 195 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7651 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2569 3286 3479 4014 4510 4911 6311 1239 Size: 1330 717 193 535 496 401 1400 2579 Fragments arranged by size: 2579 1400 1330 717 535 496 401 193 Bpu10I CC'TnA_GC Cuts at: 2112 3960 2112 Size: 1848 5803 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5776 6641 6939 5776 Size: 865 298 6488 Fragments arranged by size: 6488 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4095 6314 882 Size: 617 2596 2219 2219 Fragments arranged by size: 2596 2219 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5442 493 Size: 711 4238 2702 Fragments arranged by size: 4238 2702 711 BsaBI GATnn'nnATC Cuts at: 2247 3363 4410 7645 2247 Size: 1116 1047 3235 2253 Fragments arranged by size: 3235 2253 1116 1047 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3934 4397 5458 5840 Size: 53 83 186 936 2401 463 1061 382 Cuts at: 5840 6475 275 Size: 635 1451 Fragments arranged by size: 2401 1451 1061 936 635 463 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2859 6084 6482 6756 6903 7314 2859 Size: 3225 398 274 147 411 3196 Fragments arranged by size: 3225 3196 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2384 2414 3948 3978 1178 Size: 30 165 30 981 30 1534 30 4851 Fragments arranged by size: 4851 1534 981 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2058 3022 3386 3519 3639 3657 4011 4014 Size: 872 964 364 133 120 18 354 3 Cuts at: 4014 4017 4034 4914 1186 Size: 3 17 880 3923 Fragments arranged by size: 3923 964 880 872 364 354 133 120 18 17 3 3 BseYI C'CCAG_C Cuts at: 3637 6854 7350 3637 Size: 3217 496 3938 Fragments arranged by size: 3938 3217 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3153 4728 1163 Size: 573 1417 1575 4086 Fragments arranged by size: 4086 1575 1417 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4733 5101 5862 6890 1540 Size: 275 186 2732 368 761 1028 2301 Fragments arranged by size: 2732 2301 1028 761 368 275 186 BsiHKAI G_wGCw'C Cuts at: 3185 3416 5712 5797 6868 3185 Size: 231 2296 85 1071 3968 Fragments arranged by size: 3968 2296 1071 231 85 BsmI GAATG_Cn' Cuts at: 5162 5255 5162 Size: 93 7558 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2738 3107 3185 3264 3416 3788 3845 Size: 399 948 369 78 79 152 372 57 Cuts at: 3845 3905 4970 5712 5797 6868 1391 Size: 60 1065 742 85 1071 2174 Fragments arranged by size: 2174 1071 1065 948 742 399 372 369 152 85 79 78 60 57 BspEI T'CCGG_A Cuts at: 7314 7314 Size: 7651 BspHI T'CATG_A Cuts at: 2608 3052 5540 7270 2608 Size: 444 2488 1730 2989 Fragments arranged by size: 2989 2488 1730 444 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4351 5006 844 Size: 3507 655 3489 Fragments arranged by size: 3507 3489 655 BsrBI CCG'CTC Cuts at: 1394 1823 5538 1394 Size: 429 3715 3507 Fragments arranged by size: 3715 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2649 2683 6146 6320 65 Size: 1183 1401 34 3463 174 1396 Fragments arranged by size: 3463 1401 1396 1183 174 34 BsrFI r'CCGG_y Cuts at: 1700 1810 4533 6295 6482 1700 Size: 110 2723 1762 187 2869 Fragments arranged by size: 2869 2723 1762 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7651 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7651 BssSI C'ACGA_G Cuts at: 5711 6723 5711 Size: 1012 6639 BstAPI GCAn_nnn'nTGC Cuts at: 794 2537 3189 4811 794 Size: 1743 652 1622 3634 Fragments arranged by size: 3634 1743 1622 652 BstBI TT'CG_AA Cuts at: 6455 6455 Size: 7651 BstEII G'GTnAC_C Cuts at: 1184 3977 4626 1184 Size: 2793 649 4209 Fragments arranged by size: 4209 2793 649 BstXI CCAn_nnnn'nTGG Cuts at: 3271 3271 Size: 7651 BstYI r'GATC_y Cuts at: 1972 3274 3449 3498 3538 3563 3661 4552 4573 Size: 1302 175 49 40 25 98 891 21 Cuts at: 4573 4981 5348 5733 5750 7202 7288 7300 7646 Size: 408 367 385 17 1452 86 12 346 Cuts at: 7646 1972 Size: 1977 Fragments arranged by size: 1977 1452 1302 891 408 385 367 346 175 98 86 49 40 25 21 17 12 Bsu36I CC'TnA_GG Cuts at: 4543 4779 4543 Size: 236 7415 BtgI C'CryG_G Cuts at: 513 1065 2004 2139 2582 4771 513 Size: 552 939 135 443 2189 3393 Fragments arranged by size: 3393 2189 939 552 443 135 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6912 Fragments arranged by size: 6912 683 56 BtsI GCAGTG_nn' Cuts at: 5167 5961 5981 5167 Size: 794 20 6837 Fragments arranged by size: 6837 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7651 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7616 DraI TTT'AAA Cuts at: 2326 2380 4296 4955 2326 Size: 54 1916 659 5022 Fragments arranged by size: 5022 1916 659 54 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3149 6658 809 Size: 507 1833 3509 1802 Fragments arranged by size: 3509 1833 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4535 4597 5098 Size: 54 1425 50 275 18 2705 62 501 Cuts at: 5098 5987 7616 8 Size: 889 1629 43 Fragments arranged by size: 2705 1629 1425 889 501 275 62 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5098 1537 Size: 275 3286 4090 Fragments arranged by size: 4090 3286 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4839 4847 5581 1353 Size: 3486 8 734 3423 Fragments arranged by size: 3486 3423 734 8 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6238 6612 6758 387 Size: 1064 138 4649 374 146 1280 Fragments arranged by size: 4649 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2183 2437 2569 3286 3479 4014 4239 Size: 393 551 254 132 717 193 535 225 Cuts at: 4239 4510 4554 4911 5714 6311 7098 7366 1239 Size: 271 44 357 803 597 787 268 1524 Fragments arranged by size: 1524 803 787 717 597 551 535 393 357 271 268 254 225 193 132 44 EcoNI CCTnn'n_nnAGG Cuts at: 2609 3836 3951 4428 2609 Size: 1227 115 477 5832 Fragments arranged by size: 5832 1227 477 115 EcoRI G'AATT_C Cuts at: 2543 4162 4960 5338 2543 Size: 1619 798 378 4856 Fragments arranged by size: 4856 1619 798 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3765 4876 3765 Size: 1111 6540 FspI TGC'GCA Cuts at: 6157 6157 Size: 7651 HaeII r_GCGC'y Cuts at: 1678 6798 1678 Size: 5120 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2415 2447 2920 2952 3001 Size: 32 135 32 843 32 473 32 49 Cuts at: 3001 3033 3284 3316 3373 3405 3490 3522 3813 Size: 32 251 32 57 32 85 32 291 Cuts at: 3813 3845 3948 3980 4250 4282 4304 4336 6292 Size: 32 103 32 270 32 22 32 1956 Cuts at: 6292 6324 6366 6398 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1956 843 473 291 270 251 135 103 85 57 49 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 22 HincII GTy'rAC Cuts at: 669 1657 1938 3827 4995 5241 669 Size: 988 281 1889 1168 246 3079 Fragments arranged by size: 3079 1889 1168 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7651 HpaI GTT'AAC Cuts at: 5241 5241 Size: 7651 KpnI G_GTAC'C Cuts at: 4976 4976 Size: 7651 MfeI C'AATT_G Cuts at: 2661 5250 2661 Size: 2589 5062 MluI A'CGCG_T Cuts at: 7527 7527 Size: 7651 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2083 3192 4379 6765 6949 1305 Size: 778 1109 1187 2386 184 2007 Fragments arranged by size: 2386 2007 1187 1109 778 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4599 10 Size: 54 1425 343 2767 3062 Fragments arranged by size: 3062 2767 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3269 3338 5609 5968 6127 518 Size: 648 2103 69 2271 359 159 2042 Fragments arranged by size: 2271 2103 2042 648 359 159 69 MspA1I CmG'CkG Cuts at: 1606 5067 5744 6892 7137 1606 Size: 3461 677 1148 245 2120 Fragments arranged by size: 3461 2120 1148 677 245 NaeI GCC'GGC Cuts at: 4535 4535 Size: 7651 NcoI C'CATG_G Cuts at: 513 1065 2004 2582 4771 513 Size: 552 939 578 2189 3393 Fragments arranged by size: 3393 2189 939 578 552 NdeI CA'TA_TG Cuts at: 387 2800 387 Size: 2413 5238 NgoMIV G'CCGG_C Cuts at: 4533 4533 Size: 7651 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7651 NotI GC'GGCC_GC Cuts at: 5098 5098 Size: 7651 NspI r_CATG'y Cuts at: 1132 1229 2464 3337 5009 6554 1132 Size: 97 1235 873 1672 1545 2229 Fragments arranged by size: 2229 1672 1545 1235 873 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7651 PciI A'CATG_T Cuts at: 6550 6550 Size: 7651 PflMI CCAn_nnn'nTGG Cuts at: 1108 3736 3854 4376 1108 Size: 2628 118 522 4383 Fragments arranged by size: 4383 2628 522 118 PmeI GTTT'AAAC Cuts at: 4955 4955 Size: 7651 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5720 5752 7233 7265 5720 Size: 32 1481 32 6106 Fragments arranged by size: 6106 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7651 PsiI TTA'TAA Cuts at: 3015 5221 3015 Size: 2206 5445 PstI C_TGCA'G Cuts at: 830 5003 830 Size: 4173 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7651 SalI G'TCGA_C Cuts at: 1936 3825 4993 1936 Size: 1889 1168 4594 Fragments arranged by size: 4594 1889 1168 SbfI CC_TGCA'GG Cuts at: 5003 5003 Size: 7651 ScaI AGT'ACT Cuts at: 2321 5899 7611 2321 Size: 3578 1712 2361 Fragments arranged by size: 3578 2361 1712 SexAI A'CCwGG_T Cuts at: 4630 4630 Size: 7651 SfcI C'TryA_G Cuts at: 826 1045 2822 4999 6134 6815 7006 826 Size: 219 1777 2177 1135 681 191 1471 Fragments arranged by size: 2177 1777 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7651 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7651 SmaI CCC'GGG Cuts at: 1872 4978 1872 Size: 3106 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3655 4282 5755 6656 6918 820 Size: 197 926 1712 627 1473 901 262 1553 Fragments arranged by size: 1712 1553 1473 926 901 627 262 197 SnaBI TAC'GTA Cuts at: 493 5442 493 Size: 4949 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7651 SphI G_CATG'C Cuts at: 1132 3337 5009 1132 Size: 2205 1672 3774 Fragments arranged by size: 3774 2205 1672 SspI AAT'ATT Cuts at: 5 52 5575 5 Size: 47 5523 2081 Fragments arranged by size: 5523 2081 47 StuI AGG'CCT Cuts at: 1517 4351 1517 Size: 2834 4817 StyI C'CwwG_G Cuts at: 513 1065 2004 2582 2729 3832 3982 4771 5090 Size: 552 939 578 147 1103 150 789 319 Cuts at: 5090 513 Size: 3074 Fragments arranged by size: 3074 1103 939 789 578 552 319 150 147 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4194 5678 5695 5848 6033 6478 325 Size: 1255 2614 1484 17 153 185 445 1498 Fragments arranged by size: 2614 1498 1484 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2319 4061 5897 Size: 274 80 33 51 1443 342 1742 1836 Cuts at: 5897 7609 96 Size: 1712 138 Fragments arranged by size: 1836 1742 1712 1443 342 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3483 3515 4139 4171 7611 7643 3483 Size: 32 624 32 3440 32 3491 Fragments arranged by size: 3491 3440 624 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7651 XbaI T'CTAG_A Cuts at: 4987 4987 Size: 7651 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2627 2627 Size: 7651 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7651 XmaI C'CCGG_G Cuts at: 1870 4976 1870 Size: 3106 4545 XmnI GAAnn'nnTTC Cuts at: 2591 5780 2591 Size: 3189 4462 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5459 276 Size: 53 83 186 936 3925 2468 Fragments arranged by size: 3925 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI