(Linear) (Six-base) MAPSORT of: KIEE1387.seq Check: 8009 from: 1 to: 2946 KIEE1387.orf With 182 enzymes: SgfI * July 15, 2010 18:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2346 2946 Size: 2346 600 AccI GT'mk_AC Cuts at: 0 1716 1821 2946 Size: 1716 105 1125 Fragments arranged by size: 1716 1125 105 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 178 432 2234 2549 2946 Size: 178 254 1802 315 397 Fragments arranged by size: 1802 397 315 254 178 AflII C'TTAA_G Cuts at: 0 1650 2277 2946 Size: 1650 627 669 Fragments arranged by size: 1650 669 627 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1351 2946 Size: 1351 1595 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2226 2258 2946 Size: 2226 32 688 Fragments arranged by size: 2226 688 32 AlwNI CAG_nnn'CTG Cuts at: 0 532 2317 2946 Size: 532 1785 629 Fragments arranged by size: 1785 629 532 ApaLI G'TGCA_C Cuts at: 0 1176 1407 2946 Size: 1176 231 1539 Fragments arranged by size: 1539 1176 231 ApoI r'AATT_y Cuts at: 0 62 538 694 750 2073 2157 2747 2905 Size: 62 476 156 56 1323 84 590 158 Cuts at: 2905 2946 Size: 41 Fragments arranged by size: 1323 590 476 158 156 84 62 56 41 AvaI C'yCGr_G Cuts at: 0 1784 2354 2946 Size: 1784 570 592 Fragments arranged by size: 1784 592 570 AvrII C'CTAG_G Cuts at: 0 1827 2946 Size: 1827 1119 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 498 531 557 590 2946 Size: 498 33 26 33 2356 Fragments arranged by size: 2356 498 33 33 26 BamHI G'GATC_C Cuts at: 0 1269 1444 2946 Size: 1269 175 1502 Fragments arranged by size: 1502 1269 175 BanI G'GyrC_C Cuts at: 0 1778 2700 2946 Size: 1778 922 246 Fragments arranged by size: 1778 922 246 BanII G_rGCy'C Cuts at: 0 733 1102 1259 1840 1900 2946 Size: 733 369 157 581 60 1046 Fragments arranged by size: 1046 733 581 369 157 60 BbsI GAAGACnn'nnnn_ Cuts at: 0 148 1586 2946 Size: 148 1438 1360 Fragments arranged by size: 1438 1360 148 BciVI GTATCCnnnnn_n' Cuts at: 0 2937 2946 Size: 2937 9 BclI T'GATC_A Cuts at: 0 2667 2946 Size: 2667 279 BglI GCCn_nnn'nGGC Cuts at: 0 1164 2946 Size: 1164 1782 BglII A'GATC_T Cuts at: 0 1558 2946 Size: 1558 1388 Bme1580I G_kGCm'C Cuts at: 0 1180 1411 1783 2946 Size: 1180 231 372 1163 Fragments arranged by size: 1180 1163 372 231 BmgBI CAC'GTC Cuts at: 0 2935 2946 Size: 2935 11 BmrI ACTGGGnnnn_n' Cuts at: 0 911 1900 2095 2946 Size: 911 989 195 851 Fragments arranged by size: 989 911 851 195 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 564 1281 1474 2009 2505 2906 2946 Size: 564 717 193 535 496 401 40 Fragments arranged by size: 717 564 535 496 401 193 40 Bpu10I CC'TnA_GC Cuts at: 0 107 1955 2946 Size: 107 1848 991 Fragments arranged by size: 1848 991 107 BsaI GGTCTCn'nnnn_ Cuts at: 0 2090 2946 Size: 2090 856 BsaBI GATnn'nnATC Cuts at: 0 242 1358 2405 2946 Size: 242 1116 1047 541 Fragments arranged by size: 1116 1047 541 242 BsaHI Gr'CG_yC Cuts at: 0 1929 2392 2946 Size: 1929 463 554 Fragments arranged by size: 1929 554 463 BsaWI w'CCGG_w Cuts at: 0 854 2946 Size: 854 2092 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 379 409 1943 1973 2946 Size: 379 30 1534 30 973 Fragments arranged by size: 1534 973 379 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 53 1017 1381 1514 1634 1652 2006 2009 Size: 53 964 364 133 120 18 354 3 Cuts at: 2009 2012 2029 2909 2946 Size: 3 17 880 37 Fragments arranged by size: 964 880 364 354 133 120 53 37 18 17 3 3 BseYI C'CCAG_C Cuts at: 0 1632 2946 Size: 1632 1314 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1148 2723 2946 Size: 1148 1575 223 Fragments arranged by size: 1575 1148 223 BsiEI CG_ry'CG Cuts at: 0 2728 2946 Size: 2728 218 BsiHKAI G_wGCw'C Cuts at: 0 1180 1411 2946 Size: 1180 231 1535 Fragments arranged by size: 1535 1180 231 Bsp1286I G_dGCh'C Cuts at: 0 733 1102 1180 1259 1411 1783 1840 1900 Size: 733 369 78 79 152 372 57 60 Cuts at: 1900 2946 Size: 1046 Fragments arranged by size: 1046 733 372 369 152 79 78 60 57 BspHI T'CATG_A Cuts at: 0 603 1047 2946 Size: 603 444 1899 Fragments arranged by size: 1899 603 444 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2346 2946 Size: 2346 600 BsrDI GCAATG_nn' Cuts at: 0 644 678 2946 Size: 644 34 2268 Fragments arranged by size: 2268 644 34 BsrFI r'CCGG_y Cuts at: 0 2528 2946 Size: 2528 418 BstAPI GCAn_nnn'nTGC Cuts at: 0 532 1184 2806 2946 Size: 532 652 1622 140 Fragments arranged by size: 1622 652 532 140 BstEII G'GTnAC_C Cuts at: 0 1972 2621 2946 Size: 1972 649 325 Fragments arranged by size: 1972 649 325 BstXI CCAn_nnnn'nTGG Cuts at: 0 1266 2946 Size: 1266 1680 BstYI r'GATC_y Cuts at: 0 1269 1444 1493 1533 1558 1656 2547 2568 Size: 1269 175 49 40 25 98 891 21 Cuts at: 2568 2946 Size: 378 Fragments arranged by size: 1269 891 378 175 98 49 40 25 21 Bsu36I CC'TnA_GG Cuts at: 0 2538 2774 2946 Size: 2538 236 172 Fragments arranged by size: 2538 236 172 BtgI C'CryG_G Cuts at: 0 134 577 2766 2946 Size: 134 443 2189 180 Fragments arranged by size: 2189 443 180 134 DraI TTT'AAA Cuts at: 0 321 375 2291 2946 Size: 321 54 1916 655 Fragments arranged by size: 1916 655 321 54 DrdI GACnn_nn'nnGTC Cuts at: 0 1144 2946 Size: 1144 1802 EaeI y'GGCC_r Cuts at: 0 2530 2592 2946 Size: 2530 62 354 Fragments arranged by size: 2530 354 62 EarI CTCTTCn'nnn_ Cuts at: 0 2834 2842 2946 Size: 2834 8 104 Fragments arranged by size: 2834 104 8 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 178 432 564 1281 1474 2009 2234 2505 Size: 178 254 132 717 193 535 225 271 Cuts at: 2505 2549 2906 2946 Size: 44 357 40 Fragments arranged by size: 717 535 357 271 254 225 193 178 132 44 40 EcoNI CCTnn'n_nnAGG Cuts at: 0 604 1831 1946 2423 2946 Size: 604 1227 115 477 523 Fragments arranged by size: 1227 604 523 477 115 EcoRI G'AATT_C Cuts at: 0 538 2157 2946 Size: 538 1619 789 Fragments arranged by size: 1619 789 538 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1760 2871 2946 Size: 1760 1111 75 Fragments arranged by size: 1760 1111 75 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 410 442 915 947 996 1028 1279 1311 Size: 410 32 473 32 49 32 251 32 Cuts at: 1311 1368 1400 1485 1517 1808 1840 1943 1975 Size: 57 32 85 32 291 32 103 32 Cuts at: 1975 2245 2277 2299 2331 2946 Size: 270 32 22 32 615 Fragments arranged by size: 615 473 410 291 270 251 103 85 57 49 32 32 32 32 32 32 32 32 32 32 22 HincII GTy'rAC Cuts at: 0 1822 2946 Size: 1822 1124 MfeI C'AATT_G Cuts at: 0 656 2946 Size: 656 2290 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 78 1187 2374 2946 Size: 78 1109 1187 572 Fragments arranged by size: 1187 1109 572 78 MscI TGG'CCA Cuts at: 0 2594 2946 Size: 2594 352 MslI CAynn'nnrTG Cuts at: 0 1264 1333 2946 Size: 1264 69 1613 Fragments arranged by size: 1613 1264 69 NaeI GCC'GGC Cuts at: 0 2530 2946 Size: 2530 416 NcoI C'CATG_G Cuts at: 0 577 2766 2946 Size: 577 2189 180 Fragments arranged by size: 2189 577 180 NdeI CA'TA_TG Cuts at: 0 795 2946 Size: 795 2151 NgoMIV G'CCGG_C Cuts at: 0 2528 2946 Size: 2528 418 NspI r_CATG'y Cuts at: 0 459 1332 2946 Size: 459 873 1614 Fragments arranged by size: 1614 873 459 PflMI CCAn_nnn'nTGG Cuts at: 0 1731 1849 2371 2946 Size: 1731 118 522 575 Fragments arranged by size: 1731 575 522 118 PsiI TTA'TAA Cuts at: 0 1010 2946 Size: 1010 1936 SalI G'TCGA_C Cuts at: 0 1820 2946 Size: 1820 1126 ScaI AGT'ACT Cuts at: 0 316 2946 Size: 316 2630 SexAI A'CCwGG_T Cuts at: 0 2625 2946 Size: 2625 321 SfcI C'TryA_G Cuts at: 0 817 2946 Size: 817 2129 SmlI C'TyrA_G Cuts at: 0 1650 2277 2946 Size: 1650 627 669 Fragments arranged by size: 1650 669 627 SphI G_CATG'C Cuts at: 0 1332 2946 Size: 1332 1614 StuI AGG'CCT Cuts at: 0 2346 2946 Size: 2346 600 StyI C'CwwG_G Cuts at: 0 577 724 1827 1977 2766 2946 Size: 577 147 1103 150 789 180 Fragments arranged by size: 1103 789 577 180 150 147 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2189 2946 Size: 2189 757 TatI w'GTAC_w Cuts at: 0 314 2056 2946 Size: 314 1742 890 Fragments arranged by size: 1742 890 314 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1478 1510 2134 2166 2946 Size: 1478 32 624 32 780 Fragments arranged by size: 1478 780 624 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 622 2946 Size: 622 2324 XmnI GAAnn'nnTTC Cuts at: 0 586 2946 Size: 586 2360 Enzymes that do cut: AarI AccI AcuI AflII AlfI AloI AlwNI ApaLI ApoI AvaI AvrII BaeI BamHI BanI BanII BbsI BciVI BclI BglI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI DraI DrdI EaeI EarI Eco57MI EcoNI EcoRI FalI Hin4I HincII MfeI MmeI MscI MslI NaeI NcoI NdeI NgoMIV NspI PflMI PsiI SalI ScaI SexAI SfcI SmlI SphI StuI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflIII AgeI AhdI AleI ApaI AscI AseI AsiSI BbeI BbvCI BcgI BfrBI BlpI BmtI BplI BpuEI BsaAI BsiWI BsmI BsmBI BspEI BsrBI BsrGI BssHII BssSI BstBI BstZ17I BtgZI BtsI ClaI CspCI DraIII EagI EciI EcoICRI EcoO109I EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MluI MspA1I NarI NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmaI ZraI