(Circular) (Six-base) MAPSORT of: pFN21AB6451.seq Check: 3954 from: 1 to: 5659 pFN21AB6451 5659 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3469 278 Size: 53 83 186 936 1933 2468 Fragments arranged by size: 2468 1933 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2644 3002 1937 Size: 707 358 4594 Fragments arranged by size: 4594 707 358 Acc65I G'GTAC_C Cuts at: 2251 2980 2251 Size: 729 4930 AclI AA'CG_TT Cuts at: 1563 3786 4159 1563 Size: 2223 373 3063 Fragments arranged by size: 3063 2223 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2558 3722 5106 5374 1632 Size: 926 1164 1384 268 1917 Fragments arranged by size: 1917 1384 1164 926 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5462 AflIII A'CryG_T Cuts at: 1203 4558 5535 1203 Size: 3355 977 1327 Fragments arranged by size: 3355 1327 977 AgeI A'CCGG_T Cuts at: 4490 4490 Size: 5659 AhdI GACnn_n'nnGTC Cuts at: 4388 4388 Size: 5659 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5659 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5659 AlwNI CAG_nnn'CTG Cuts at: 2171 2329 2825 3402 4974 2171 Size: 158 496 577 1572 2856 Fragments arranged by size: 2856 1572 577 496 158 ApaI G_GGCC'C Cuts at: 2130 2130 Size: 5659 ApaLI G'TGCA_C Cuts at: 3716 4872 3716 Size: 1156 4503 ApoI r'AATT_y Cuts at: 1493 2778 2799 2968 3194 3346 5455 1493 Size: 1285 21 169 226 152 2109 1697 Fragments arranged by size: 2109 1697 1285 226 169 152 21 AseI AT'TA_AT Cuts at: 160 1032 4213 160 Size: 872 3181 1606 Fragments arranged by size: 3181 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 AvaI C'yCGr_G Cuts at: 1870 1943 2984 1870 Size: 73 1041 4545 Fragments arranged by size: 4545 1041 73 BamHI G'GATC_C Cuts at: 2989 3356 2989 Size: 367 5292 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2251 2634 2888 2980 4435 Size: 325 217 627 464 383 254 92 1455 Cuts at: 4435 618 Size: 1842 Fragments arranged by size: 1842 1455 627 464 383 325 254 217 92 BanII G_rGCy'C Cuts at: 1391 2130 2978 1391 Size: 739 848 4072 Fragments arranged by size: 4072 848 739 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4688 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3850 3884 1903 Size: 34 1913 34 3678 Fragments arranged by size: 3678 1913 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2076 2088 3551 4761 2076 Size: 12 1463 1210 2974 Fragments arranged by size: 2974 1463 1210 12 BclI T'GATC_A Cuts at: 1548 1548 Size: 5659 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2181 4270 136 Size: 107 122 71 631 605 509 2089 1525 Fragments arranged by size: 2089 1525 631 605 509 122 107 71 BglII A'GATC_T Cuts at: 5654 5654 Size: 5659 BlpI GC'TnA_GC Cuts at: 3076 3076 Size: 5659 Bme1580I G_kGCm'C Cuts at: 1790 2130 3720 4876 1790 Size: 340 1590 1156 2573 Fragments arranged by size: 2573 1590 1156 340 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5659 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3390 4348 442 Size: 416 53 83 399 24 1973 958 1753 Fragments arranged by size: 1973 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5659 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2088 2549 4319 1239 Size: 849 461 1770 2579 Fragments arranged by size: 2579 1770 849 461 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2509 3784 4649 4947 2509 Size: 1275 865 298 3221 Fragments arranged by size: 3221 1275 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2527 4322 882 Size: 617 1028 1795 2219 Fragments arranged by size: 2219 1795 1028 617 BsaAI yAC'GTr Cuts at: 493 1204 3450 493 Size: 711 2246 2702 Fragments arranged by size: 2702 2246 711 BsaBI GATnn'nnATC Cuts at: 2585 5653 2585 Size: 3068 2591 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3466 3848 4483 275 Size: 53 83 186 936 1933 382 635 1451 Fragments arranged by size: 1933 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4092 4490 4764 4911 5322 4092 Size: 398 274 147 411 4429 Fragments arranged by size: 4429 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2943 2973 1178 Size: 30 165 30 1540 30 3864 Fragments arranged by size: 3864 1540 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2061 2586 1186 Size: 875 525 4259 Fragments arranged by size: 4259 875 525 BseYI C'CCAG_C Cuts at: 2176 4862 5358 2176 Size: 2686 496 2477 Fragments arranged by size: 2686 2477 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2734 1163 Size: 573 998 4088 Fragments arranged by size: 4088 998 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3109 3870 4898 1540 Size: 275 186 1108 761 1028 2301 Fragments arranged by size: 2301 1108 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3720 3805 4876 3720 Size: 85 1071 4503 Fragments arranged by size: 4503 1071 85 BsmI GAATG_Cn' Cuts at: 2035 2090 3170 3263 2035 Size: 55 1080 93 4431 Fragments arranged by size: 4431 1080 93 55 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2130 2978 3720 3805 4876 1391 Size: 399 340 848 742 85 1071 2174 Fragments arranged by size: 2174 1071 848 742 399 340 85 BspEI T'CCGG_A Cuts at: 5322 5322 Size: 5659 BspHI T'CATG_A Cuts at: 3548 5278 3548 Size: 1730 3929 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3014 844 Size: 2170 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3546 1394 Size: 429 1723 3507 Fragments arranged by size: 3507 1723 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4154 4328 65 Size: 1183 2906 174 1396 Fragments arranged by size: 2906 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2156 4303 4490 1700 Size: 110 346 2147 187 2869 Fragments arranged by size: 2869 2147 346 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5659 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5659 BssSI C'ACGA_G Cuts at: 3719 4731 3719 Size: 1012 4647 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5659 BstBI TT'CG_AA Cuts at: 4463 4463 Size: 5659 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5659 BstYI r'GATC_y Cuts at: 1972 2989 3356 3741 3758 5210 5296 5308 5654 Size: 1017 367 385 17 1452 86 12 346 Cuts at: 5654 1972 Size: 1977 Fragments arranged by size: 1977 1452 1017 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2255 513 Size: 552 939 251 3917 Fragments arranged by size: 3917 939 552 251 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4920 Fragments arranged by size: 4920 683 56 BtsI GCAGTG_nn' Cuts at: 3175 3969 3989 3175 Size: 794 20 4845 Fragments arranged by size: 4845 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5659 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5624 DraI TTT'AAA Cuts at: 2963 2963 Size: 5659 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4666 809 Size: 507 3350 1802 Fragments arranged by size: 3350 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2158 3106 3995 Size: 54 1425 50 275 18 328 948 889 Cuts at: 3995 5624 8 Size: 1629 43 Fragments arranged by size: 1629 1425 948 889 328 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3106 1537 Size: 275 1294 4090 Fragments arranged by size: 4090 1294 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3589 1353 Size: 2236 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4246 4620 4766 387 Size: 1064 138 2657 374 146 1280 Fragments arranged by size: 2657 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2088 2549 2558 3722 4319 5106 5374 Size: 393 456 461 9 1164 597 787 268 Cuts at: 5374 1239 Size: 1524 Fragments arranged by size: 1524 1164 787 597 461 456 393 268 9 EcoO109I rG'GnC_Cy Cuts at: 2099 2127 2168 2099 Size: 28 41 5590 Fragments arranged by size: 5590 41 28 EcoRI G'AATT_C Cuts at: 2968 3346 2968 Size: 378 5281 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2422 2422 Size: 5659 FspI TGC'GCA Cuts at: 4165 4165 Size: 5659 HaeII r_GCGC'y Cuts at: 1678 2270 4806 1678 Size: 592 2536 2531 Fragments arranged by size: 2536 2531 592 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2732 2734 2764 2766 4300 Size: 32 135 32 1160 2 30 2 1534 Cuts at: 4300 4332 4374 4406 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1534 1160 135 42 32 32 32 32 30 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 3003 3249 669 Size: 988 281 1065 246 3079 Fragments arranged by size: 3079 1065 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5659 HpaI GTT'AAC Cuts at: 3249 3249 Size: 5659 KpnI G_GTAC'C Cuts at: 2255 2984 2255 Size: 729 4930 MfeI C'AATT_G Cuts at: 3258 3258 Size: 5659 MluI A'CGCG_T Cuts at: 5535 5535 Size: 5659 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2218 4773 4957 1305 Size: 913 2555 184 2007 Fragments arranged by size: 2555 2007 913 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3837 Fragments arranged by size: 3837 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2542 3617 3976 4135 518 Size: 648 1376 1075 359 159 2042 Fragments arranged by size: 2042 1376 1075 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2180 2432 2553 2718 3075 3752 4900 5145 Size: 574 252 121 165 357 677 1148 245 Cuts at: 5145 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 574 357 252 245 165 121 NaeI GCC'GGC Cuts at: 2158 2158 Size: 5659 NcoI C'CATG_G Cuts at: 513 1065 2004 2255 513 Size: 552 939 251 3917 Fragments arranged by size: 3917 939 552 251 NdeI CA'TA_TG Cuts at: 387 387 Size: 5659 NgoMIV G'CCGG_C Cuts at: 2156 2156 Size: 5659 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5659 NotI GC'GGCC_GC Cuts at: 3106 3106 Size: 5659 NspI r_CATG'y Cuts at: 1132 1229 3017 4562 1132 Size: 97 1788 1545 2229 Fragments arranged by size: 2229 1788 1545 97 PasI CC'CwG_GG Cuts at: 1348 2123 1348 Size: 775 4884 PciI A'CATG_T Cuts at: 4558 4558 Size: 5659 PflMI CCAn_nnn'nTGG Cuts at: 1108 2790 1108 Size: 1682 3977 PfoI T'CCnGG_A Cuts at: 2104 2104 Size: 5659 PmeI GTTT'AAAC Cuts at: 2963 2963 Size: 5659 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3728 3760 5241 5273 3728 Size: 32 1481 32 4114 Fragments arranged by size: 4114 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2099 2168 2099 Size: 69 5590 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5659 PsiI TTA'TAA Cuts at: 3229 3229 Size: 5659 PspOMI G'GGCC_C Cuts at: 2126 2126 Size: 5659 PstI C_TGCA'G Cuts at: 830 2248 3011 830 Size: 1418 763 3478 Fragments arranged by size: 3478 1418 763 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5659 PvuII CAG'CTG Cuts at: 2180 2553 2718 2180 Size: 373 165 5121 Fragments arranged by size: 5121 373 165 SalI G'TCGA_C Cuts at: 1936 3001 1936 Size: 1065 4594 SbfI CC_TGCA'GG Cuts at: 3011 3011 Size: 5659 ScaI AGT'ACT Cuts at: 3907 5619 3907 Size: 1712 3947 SfcI C'TryA_G Cuts at: 826 1045 2244 3007 4142 4823 5014 826 Size: 219 1199 763 1135 681 191 1471 Fragments arranged by size: 1471 1199 1135 763 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5659 SmaI CCC'GGG Cuts at: 1872 2986 1872 Size: 1114 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2488 3763 4664 4926 820 Size: 197 926 545 1275 901 262 1553 Fragments arranged by size: 1553 1275 926 901 545 262 197 SnaBI TAC'GTA Cuts at: 493 3450 493 Size: 2957 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5659 SphI G_CATG'C Cuts at: 1132 3017 1132 Size: 1885 3774 SspI AAT'ATT Cuts at: 5 52 2306 3583 5 Size: 47 2254 1277 2081 Fragments arranged by size: 2254 2081 1277 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5659 StyI C'CwwG_G Cuts at: 513 1065 2004 2095 2255 3098 513 Size: 552 939 91 160 843 3074 Fragments arranged by size: 3074 939 843 552 160 91 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2652 3686 3703 3856 4041 4486 325 Size: 1255 1072 1034 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1072 1034 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2683 3905 5617 Size: 274 80 33 51 1443 706 1222 1712 Cuts at: 5617 96 Size: 138 Fragments arranged by size: 1712 1443 1222 706 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5619 5651 5619 Size: 32 5627 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5659 XbaI T'CTAG_A Cuts at: 2474 2995 2474 Size: 521 5138 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2168 2597 2168 Size: 429 5230 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5659 XmaI C'CCGG_G Cuts at: 1870 2984 1870 Size: 1114 4545 XmnI GAAnn'nnTTC Cuts at: 3788 3788 Size: 5659 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3467 276 Size: 53 83 186 936 1933 2468 Fragments arranged by size: 2468 1933 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI