(Circular) (Six-base) MAPSORT of: pFN21AE1046.seq Check: 3200 from: 1 to: 5140 pFN21AE1046 5140 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2950 278 Size: 53 83 186 936 1414 2468 Fragments arranged by size: 2468 1414 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2483 1937 Size: 546 4594 Acc65I G'GTAC_C Cuts at: 2461 2461 Size: 5140 AclI AA'CG_TT Cuts at: 1563 3267 3640 1563 Size: 1704 373 3063 Fragments arranged by size: 3063 1704 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3203 4587 4855 1632 Size: 1571 1384 268 1917 Fragments arranged by size: 1917 1571 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4943 AflIII A'CryG_T Cuts at: 1203 4039 5016 1203 Size: 2836 977 1327 Fragments arranged by size: 2836 1327 977 AgeI A'CCGG_T Cuts at: 3971 3971 Size: 5140 AhdI GACnn_n'nnGTC Cuts at: 3869 3869 Size: 5140 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5140 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5140 AlwNI CAG_nnn'CTG Cuts at: 2883 4455 2883 Size: 1572 3568 ApaLI G'TGCA_C Cuts at: 3197 4353 3197 Size: 1156 3984 ApoI r'AATT_y Cuts at: 1493 2449 2675 2827 4936 1493 Size: 956 226 152 2109 1697 Fragments arranged by size: 2109 1697 956 226 152 AseI AT'TA_AT Cuts at: 160 1032 3694 160 Size: 872 2662 1606 Fragments arranged by size: 2662 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5140 AvaI C'yCGr_G Cuts at: 1870 1943 2430 2465 1870 Size: 73 487 35 4545 Fragments arranged by size: 4545 487 73 35 BamHI G'GATC_C Cuts at: 2470 2837 2470 Size: 367 4773 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2067 2461 3916 618 Size: 325 217 627 280 394 1455 1842 Fragments arranged by size: 1842 1455 627 394 325 280 217 BanII G_rGCy'C Cuts at: 1391 2379 2459 1391 Size: 988 80 4072 Fragments arranged by size: 4072 988 80 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4169 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3331 3365 1903 Size: 34 1394 34 3678 Fragments arranged by size: 3678 1394 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3032 4242 3032 Size: 1210 3930 BclI T'GATC_A Cuts at: 1548 1548 Size: 5140 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2355 3751 136 Size: 107 122 71 631 605 683 1396 1525 Fragments arranged by size: 1525 1396 683 631 605 122 107 71 BglII A'GATC_T Cuts at: 5135 5135 Size: 5140 BlpI GC'TnA_GC Cuts at: 2557 2557 Size: 5140 Bme1580I G_kGCm'C Cuts at: 1790 2351 3201 4357 1790 Size: 561 850 1156 2573 Fragments arranged by size: 2573 1156 850 561 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5140 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2247 2871 3829 Size: 416 53 83 399 24 830 624 958 Cuts at: 3829 442 Size: 1753 Fragments arranged by size: 1753 958 830 624 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5140 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3800 1239 Size: 2561 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3265 4130 4428 3265 Size: 865 298 3977 Fragments arranged by size: 3977 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3803 882 Size: 617 2304 2219 Fragments arranged by size: 2304 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2931 493 Size: 711 1727 2702 Fragments arranged by size: 2702 1727 711 BsaBI GATnn'nnATC Cuts at: 5134 5134 Size: 5140 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2231 2947 3329 3964 Size: 53 83 186 936 698 716 382 635 Cuts at: 3964 275 Size: 1451 Fragments arranged by size: 1451 936 716 698 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2034 3573 3971 4245 4392 4803 2034 Size: 1539 398 274 147 411 2371 Fragments arranged by size: 2371 1539 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2090 2120 1178 Size: 30 165 30 687 30 4198 Fragments arranged by size: 4198 687 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2114 2117 1186 Size: 928 3 4209 Fragments arranged by size: 4209 928 3 BseYI C'CCAG_C Cuts at: 2353 4343 4839 2353 Size: 1990 496 2654 Fragments arranged by size: 2654 1990 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2036 2279 1163 Size: 573 300 243 4024 Fragments arranged by size: 4024 573 300 243 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2590 3351 4379 1540 Size: 275 186 589 761 1028 2301 Fragments arranged by size: 2301 1028 761 589 275 186 BsiHKAI G_wGCw'C Cuts at: 2379 3201 3286 4357 2379 Size: 822 85 1071 3162 Fragments arranged by size: 3162 1071 822 85 BsmI GAATG_Cn' Cuts at: 2651 2744 2651 Size: 93 5047 BsmBI CGTCTCn'nnnn_ Cuts at: 2222 2222 Size: 5140 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2351 2379 2459 3201 3286 4357 1391 Size: 399 561 28 80 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 561 399 85 80 28 BspEI T'CCGG_A Cuts at: 4803 4803 Size: 5140 BspHI T'CATG_A Cuts at: 2253 3029 4759 2253 Size: 776 1730 2634 Fragments arranged by size: 2634 1730 776 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2495 844 Size: 1651 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2142 3027 1394 Size: 429 319 885 3507 Fragments arranged by size: 3507 885 429 319 BsrDI GCAATG_nn' Cuts at: 65 1248 3635 3809 65 Size: 1183 2387 174 1396 Fragments arranged by size: 2387 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3784 3971 1700 Size: 110 1974 187 2869 Fragments arranged by size: 2869 1974 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5140 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5140 BssSI C'ACGA_G Cuts at: 3200 4212 3200 Size: 1012 4128 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5140 BstBI TT'CG_AA Cuts at: 3944 3944 Size: 5140 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5140 BstYI r'GATC_y Cuts at: 1972 2470 2837 3222 3239 4691 4777 4789 5135 Size: 498 367 385 17 1452 86 12 346 Cuts at: 5135 1972 Size: 1977 Fragments arranged by size: 1977 1452 498 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4588 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4401 Fragments arranged by size: 4401 683 56 BtsI GCAGTG_nn' Cuts at: 2656 3450 3470 2656 Size: 794 20 4326 Fragments arranged by size: 4326 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5140 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5105 DraI TTT'AAA Cuts at: 2444 2444 Size: 5140 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4147 809 Size: 507 2831 1802 Fragments arranged by size: 2831 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2587 3476 5105 Size: 54 1425 50 275 18 757 889 1629 Cuts at: 5105 8 Size: 43 Fragments arranged by size: 1629 1425 889 757 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2587 1537 Size: 275 775 4090 Fragments arranged by size: 4090 775 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2135 3070 1353 Size: 782 935 3423 Fragments arranged by size: 3423 935 782 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2416 3727 4101 4247 387 Size: 1064 138 827 1311 374 146 1280 Fragments arranged by size: 1311 1280 1064 827 374 146 138 EcoICRI GAG'CTC Cuts at: 2377 2377 Size: 5140 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3203 3800 4587 4855 1239 Size: 393 1571 597 787 268 1524 Fragments arranged by size: 1571 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2276 2276 Size: 5140 EcoRI G'AATT_C Cuts at: 2449 2827 2449 Size: 378 4762 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2200 2200 Size: 5140 FspI TGC'GCA Cuts at: 3646 3646 Size: 5140 HaeII r_GCGC'y Cuts at: 1678 4287 1678 Size: 2609 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3781 3813 3855 3887 1373 Size: 32 135 32 2209 32 42 32 2626 Fragments arranged by size: 2626 2209 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2484 2730 669 Size: 988 281 546 246 3079 Fragments arranged by size: 3079 988 546 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5140 HpaI GTT'AAC Cuts at: 2730 2730 Size: 5140 KpnI G_GTAC'C Cuts at: 2465 2465 Size: 5140 MfeI C'AATT_G Cuts at: 2739 2739 Size: 5140 MluI A'CGCG_T Cuts at: 5016 5016 Size: 5140 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4254 4438 1305 Size: 2949 184 2007 Fragments arranged by size: 2949 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3318 Fragments arranged by size: 3318 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3098 3457 3616 518 Size: 648 1932 359 159 2042 Fragments arranged by size: 2042 1932 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2353 2556 3233 4381 4626 1606 Size: 747 203 677 1148 245 2120 Fragments arranged by size: 2120 1148 747 677 245 203 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4588 NdeI CA'TA_TG Cuts at: 387 387 Size: 5140 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5140 NotI GC'GGCC_GC Cuts at: 2587 2587 Size: 5140 NspI r_CATG'y Cuts at: 1132 1229 2498 4043 1132 Size: 97 1269 1545 2229 Fragments arranged by size: 2229 1545 1269 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5140 PciI A'CATG_T Cuts at: 4039 4039 Size: 5140 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5140 PmeI GTTT'AAAC Cuts at: 2444 2444 Size: 5140 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3209 3241 4722 4754 3209 Size: 32 1481 32 3595 Fragments arranged by size: 3595 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2276 2276 Size: 5140 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5140 PsiI TTA'TAA Cuts at: 2710 2710 Size: 5140 PstI C_TGCA'G Cuts at: 830 2492 830 Size: 1662 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5140 SacI G_AGCT'C Cuts at: 2379 2379 Size: 5140 SalI G'TCGA_C Cuts at: 1936 2482 1936 Size: 546 4594 SanDI GG'GwC_CC Cuts at: 2276 2276 Size: 5140 SapI GCTCTTCn'nnn_ Cuts at: 2135 2135 Size: 5140 SbfI CC_TGCA'GG Cuts at: 2492 2492 Size: 5140 ScaI AGT'ACT Cuts at: 3388 5100 3388 Size: 1712 3428 SfcI C'TryA_G Cuts at: 826 1045 2056 2305 2488 3623 4304 4495 826 Size: 219 1011 249 183 1135 681 191 1471 Fragments arranged by size: 1471 1135 1011 681 249 219 191 183 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5140 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5140 SmaI CCC'GGG Cuts at: 1872 2432 2467 1872 Size: 560 35 4545 Fragments arranged by size: 4545 560 35 SmlI C'TyrA_G Cuts at: 820 1017 1943 3244 4145 4407 820 Size: 197 926 1301 901 262 1553 Fragments arranged by size: 1553 1301 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2931 493 Size: 2438 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5140 SphI G_CATG'C Cuts at: 1132 2498 1132 Size: 1366 3774 SrfI GCCC'GGGC Cuts at: 2432 2432 Size: 5140 SspI AAT'ATT Cuts at: 5 52 3064 5 Size: 47 3012 2081 Fragments arranged by size: 3012 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5140 StyI C'CwwG_G Cuts at: 513 1065 2094 2579 513 Size: 552 1029 485 3074 Fragments arranged by size: 3074 1029 552 485 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3167 3184 3337 3522 3967 325 Size: 1255 1587 17 153 185 445 1498 Fragments arranged by size: 1587 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3386 5098 96 Size: 274 80 33 51 1443 1409 1712 138 Fragments arranged by size: 1712 1443 1409 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5100 5132 5100 Size: 32 5108 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5140 XbaI T'CTAG_A Cuts at: 2476 2476 Size: 5140 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5140 XmaI C'CCGG_G Cuts at: 1870 2430 2465 1870 Size: 560 35 4545 Fragments arranged by size: 4545 560 35 XmnI GAAnn'nnTTC Cuts at: 3269 3269 Size: 5140 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2948 276 Size: 53 83 186 936 1414 2468 Fragments arranged by size: 2468 1414 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SexAI SfiI SfoI SwaI XcmI