(Circular) (Six-base) MAPSORT of: pFN21AB7996.seq Check: 9081 from: 1 to: 6115 pFN21AB7996 6115 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2491 2713 2734 2743 2491 Size: 222 21 9 5863 Fragments arranged by size: 5863 222 21 9 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3925 278 Size: 53 83 186 936 2389 2468 Fragments arranged by size: 2468 2389 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3458 1937 Size: 1521 4594 Acc65I G'GTAC_C Cuts at: 3436 3436 Size: 6115 AclI AA'CG_TT Cuts at: 1563 4242 4615 1563 Size: 2679 373 3063 Fragments arranged by size: 3063 2679 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2023 4178 5562 5830 1632 Size: 391 2155 1384 268 1917 Fragments arranged by size: 2155 1917 1384 391 268 AfeI AGC'GCT Cuts at: 2796 2796 Size: 6115 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5918 AflIII A'CryG_T Cuts at: 1203 5014 5991 1203 Size: 3811 977 1327 Fragments arranged by size: 3811 1327 977 AgeI A'CCGG_T Cuts at: 4946 4946 Size: 6115 AhdI GACnn_n'nnGTC Cuts at: 4844 4844 Size: 6115 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6115 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2153 1184 Size: 969 5146 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3026 3058 3026 Size: 32 6083 AlwNI CAG_nnn'CTG Cuts at: 2305 2446 3858 5430 2305 Size: 141 1412 1572 2990 Fragments arranged by size: 2990 1572 1412 141 ApaLI G'TGCA_C Cuts at: 4172 5328 4172 Size: 1156 4959 ApoI r'AATT_y Cuts at: 1493 3424 3650 3802 5911 1493 Size: 1931 226 152 2109 1697 Fragments arranged by size: 2109 1931 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4669 160 Size: 872 3637 1606 Fragments arranged by size: 3637 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6115 AvaI C'yCGr_G Cuts at: 1870 1943 2090 2155 2193 2400 2780 3086 3440 Size: 73 147 65 38 207 380 306 354 Cuts at: 3440 1870 Size: 4545 Fragments arranged by size: 4545 380 354 306 207 147 73 65 38 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2044 2077 2044 Size: 33 6082 BamHI G'GATC_C Cuts at: 3445 3812 3445 Size: 367 5748 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2207 3126 3436 4891 618 Size: 325 217 627 420 919 310 1455 1842 Fragments arranged by size: 1842 1455 919 627 420 325 310 217 BanII G_rGCy'C Cuts at: 1391 2147 2162 2316 3150 3434 1391 Size: 756 15 154 834 284 4072 Fragments arranged by size: 4072 834 756 284 154 15 BbeI G_GCGC'C Cuts at: 2211 2211 Size: 6115 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3317 3390 928 Size: 971 1418 73 3653 Fragments arranged by size: 3653 1418 971 73 BbvCI CC'TCA_GC Cuts at: 2798 2798 Size: 6115 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2824 2858 4306 4340 1903 Size: 34 887 34 1448 34 3678 Fragments arranged by size: 3678 1448 887 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4007 5217 4007 Size: 1210 4905 BclI T'GATC_A Cuts at: 1548 3321 1548 Size: 1773 4342 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2142 3053 4726 Size: 107 122 71 631 605 470 911 1673 Cuts at: 4726 136 Size: 1525 Fragments arranged by size: 1673 1525 911 631 605 470 122 107 71 BglII A'GATC_T Cuts at: 6110 6110 Size: 6115 BlpI GC'TnA_GC Cuts at: 2390 3532 2390 Size: 1142 4973 Bme1580I G_kGCm'C Cuts at: 1790 3234 4176 5332 1790 Size: 1444 942 1156 2573 Fragments arranged by size: 2573 1444 1156 942 BmgBI CAC'GTC Cuts at: 1318 2614 1318 Size: 1296 4819 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3846 4804 442 Size: 416 53 83 399 24 2429 958 1753 Fragments arranged by size: 2429 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6115 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2110 2378 2489 3053 3071 3224 4775 1239 Size: 871 268 111 564 18 153 1551 2579 Fragments arranged by size: 2579 1551 871 564 268 153 111 18 Bpu10I CC'TnA_GC Cuts at: 2798 3014 2798 Size: 216 5899 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2525 2672 4240 5105 5403 2525 Size: 147 1568 865 298 3237 Fragments arranged by size: 3237 1568 865 298 147 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2709 3329 4778 882 Size: 617 1210 620 1449 2219 Fragments arranged by size: 2219 1449 1210 620 617 BsaAI yAC'GTr Cuts at: 493 1204 3906 493 Size: 711 2702 2702 Fragments arranged by size: 2702 2702 711 BsaBI GATnn'nnATC Cuts at: 6109 6109 Size: 6115 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2208 2953 3922 4304 Size: 53 83 186 936 675 745 969 382 Cuts at: 4304 4939 275 Size: 635 1451 Fragments arranged by size: 1451 969 936 745 675 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2901 4548 4946 5220 5367 5778 2901 Size: 1647 398 274 147 411 3238 Fragments arranged by size: 3238 1647 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2846 2876 3026 3056 1178 Size: 30 165 30 1443 30 150 30 4237 Fragments arranged by size: 4237 1443 165 150 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2152 2570 2573 2717 2720 2870 3050 3104 Size: 966 418 3 144 3 150 180 54 Cuts at: 3104 3107 3128 3140 3227 1186 Size: 3 21 12 87 4074 Fragments arranged by size: 4074 966 418 180 150 144 87 54 21 12 3 3 3 BseYI C'CCAG_C Cuts at: 2140 5318 5814 2140 Size: 3178 496 2441 Fragments arranged by size: 3178 2441 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2519 2681 2768 1163 Size: 573 783 162 87 4510 Fragments arranged by size: 4510 783 573 162 87 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2323 3565 4326 5354 1540 Size: 275 186 322 1242 761 1028 2301 Fragments arranged by size: 2301 1242 1028 761 322 275 186 BsiHKAI G_wGCw'C Cuts at: 2162 2316 2589 2601 3150 4176 4261 5332 2162 Size: 154 273 12 549 1026 85 1071 2945 Fragments arranged by size: 2945 1071 1026 549 273 154 85 12 BsmI GAATG_Cn' Cuts at: 3626 3719 3626 Size: 93 6022 BsmBI CGTCTCn'nnnn_ Cuts at: 2555 2555 Size: 6115 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2147 2162 2316 2589 2601 3150 3234 Size: 399 357 15 154 273 12 549 84 Cuts at: 3234 3434 4176 4261 5332 1391 Size: 200 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 549 399 357 273 200 154 85 84 15 12 BspEI T'CCGG_A Cuts at: 5778 5778 Size: 6115 BspHI T'CATG_A Cuts at: 4004 5734 4004 Size: 1730 4385 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2491 2713 2727 2734 2743 2850 3470 844 Size: 1647 222 14 7 9 107 620 3489 Fragments arranged by size: 3489 1647 620 222 107 14 9 7 BsrBI CCG'CTC Cuts at: 1394 1823 2198 2204 4002 1394 Size: 429 375 6 1798 3507 Fragments arranged by size: 3507 1798 429 375 6 BsrDI GCAATG_nn' Cuts at: 65 1248 4610 4784 65 Size: 1183 3362 174 1396 Fragments arranged by size: 3362 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2033 2413 4759 4946 1700 Size: 110 223 380 2346 187 2869 Fragments arranged by size: 2869 2346 380 223 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6115 BssHII G'CGCG_C Cuts at: 1925 2417 2419 2811 1925 Size: 492 2 392 5229 Fragments arranged by size: 5229 492 392 2 BssSI C'ACGA_G Cuts at: 2594 2699 2909 4175 5187 2594 Size: 105 210 1266 1012 3522 Fragments arranged by size: 3522 1266 1012 210 105 BstAPI GCAn_nnn'nTGC Cuts at: 794 2305 2446 794 Size: 1511 141 4463 Fragments arranged by size: 4463 1511 141 BstBI TT'CG_AA Cuts at: 4919 4919 Size: 6115 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6115 BstYI r'GATC_y Cuts at: 1972 2958 3222 3445 3812 4197 4214 5666 5752 Size: 986 264 223 367 385 17 1452 86 Cuts at: 5752 5764 6110 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 986 385 367 346 264 223 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3131 3276 3131 Size: 145 5970 BtgI C'CryG_G Cuts at: 513 1065 2247 2769 513 Size: 552 1182 522 3859 Fragments arranged by size: 3859 1182 552 522 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2812 487 Size: 683 56 1586 3790 Fragments arranged by size: 3790 1586 683 56 BtsI GCAGTG_nn' Cuts at: 3388 3631 4425 4445 3388 Size: 243 794 20 5058 Fragments arranged by size: 5058 794 243 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6115 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6080 DraI TTT'AAA Cuts at: 3419 3419 Size: 6115 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5122 809 Size: 507 3806 1802 Fragments arranged by size: 3806 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2250 2277 3562 Size: 54 1425 50 275 18 420 27 1285 Cuts at: 3562 4451 6080 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1285 889 420 275 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 3562 1537 Size: 275 1750 4090 Fragments arranged by size: 4090 1750 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2606 4045 1353 Size: 1253 1439 3423 Fragments arranged by size: 3423 1439 1253 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2537 2867 4702 5076 5222 387 Size: 1064 138 948 330 1835 374 146 1280 Fragments arranged by size: 1835 1280 1064 948 374 330 146 138 EcoICRI GAG'CTC Cuts at: 2160 2314 3148 2160 Size: 154 834 5127 Fragments arranged by size: 5127 834 154 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2023 2110 2378 2489 3053 3071 3224 Size: 393 391 87 268 111 564 18 153 Cuts at: 3224 4178 4775 5562 5830 1239 Size: 954 597 787 268 1524 Fragments arranged by size: 1524 954 787 597 564 393 391 268 268 153 111 87 18 EcoNI CCTnn'n_nnAGG Cuts at: 2264 2846 2921 2264 Size: 582 75 5458 Fragments arranged by size: 5458 582 75 EcoO109I rG'GnC_Cy Cuts at: 2094 2257 2527 2914 2094 Size: 163 270 387 5295 Fragments arranged by size: 5295 387 270 163 EcoRI G'AATT_C Cuts at: 3424 3802 3424 Size: 378 5737 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2368 2368 Size: 6115 FspI TGC'GCA Cuts at: 4621 4621 Size: 6115 HaeII r_GCGC'y Cuts at: 1678 2211 2236 2540 2798 3011 3078 5262 1678 Size: 533 25 304 258 213 67 2184 2531 Fragments arranged by size: 2531 2184 533 304 258 213 67 25 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2647 2679 2943 2950 2975 Size: 32 135 32 1075 32 264 7 25 Cuts at: 2975 2982 4756 4788 4830 4862 1373 Size: 7 1774 32 42 32 2626 Fragments arranged by size: 2626 1774 1075 264 135 42 32 32 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 3459 3705 669 Size: 988 281 1521 246 3079 Fragments arranged by size: 3079 1521 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6115 HpaI GTT'AAC Cuts at: 3705 3705 Size: 6115 KasI G'GCGC_C Cuts at: 2207 2207 Size: 6115 KpnI G_GTAC'C Cuts at: 3440 3440 Size: 6115 MfeI C'AATT_G Cuts at: 3714 3714 Size: 6115 MluI A'CGCG_T Cuts at: 5991 5991 Size: 6115 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2532 2913 5229 5413 1305 Size: 1227 381 2316 184 2007 Fragments arranged by size: 2316 2007 1227 381 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2279 10 Size: 54 1425 343 447 3846 Fragments arranged by size: 3846 1425 447 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4073 4432 4591 518 Size: 648 2907 359 159 2042 Fragments arranged by size: 2907 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2431 3531 4208 5356 5601 1606 Size: 825 1100 677 1148 245 2120 Fragments arranged by size: 2120 1148 1100 825 677 245 NaeI GCC'GGC Cuts at: 2035 2415 2035 Size: 380 5735 NarI GG'CG_CC Cuts at: 2208 2208 Size: 6115 NcoI C'CATG_G Cuts at: 513 1065 2247 513 Size: 552 1182 4381 Fragments arranged by size: 4381 1182 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6115 NgoMIV G'CCGG_C Cuts at: 2033 2413 2033 Size: 380 5735 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6115 NotI GC'GGCC_GC Cuts at: 3562 3562 Size: 6115 NspI r_CATG'y Cuts at: 1132 1229 3473 5018 1132 Size: 97 2244 1545 2229 Fragments arranged by size: 2244 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6115 PciI A'CATG_T Cuts at: 5014 5014 Size: 6115 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6115 PmeI GTTT'AAAC Cuts at: 3419 3419 Size: 6115 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2666 2698 3026 3058 4184 4216 5697 5729 2666 Size: 32 328 32 1126 32 1481 32 3052 Fragments arranged by size: 3052 1481 1126 328 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2527 2527 Size: 6115 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6115 PsiI TTA'TAA Cuts at: 3685 3685 Size: 6115 PspXI vC'TCGA_Gb Cuts at: 3086 3086 Size: 6115 PstI C_TGCA'G Cuts at: 830 2310 2724 2847 3467 830 Size: 1480 414 123 620 3478 Fragments arranged by size: 3478 1480 620 414 123 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6115 PvuII CAG'CTG Cuts at: 2431 2431 Size: 6115 SacI G_AGCT'C Cuts at: 2162 2316 3150 2162 Size: 154 834 5127 Fragments arranged by size: 5127 834 154 SalI G'TCGA_C Cuts at: 1936 3457 1936 Size: 1521 4594 SapI GCTCTTCn'nnn_ Cuts at: 2606 2606 Size: 6115 SbfI CC_TGCA'GG Cuts at: 2724 2847 3467 2724 Size: 123 620 5372 Fragments arranged by size: 5372 620 123 ScaI AGT'ACT Cuts at: 4363 6075 4363 Size: 1712 4403 SfcI C'TryA_G Cuts at: 826 1045 2306 2720 2843 3463 4598 5279 5470 Size: 219 1261 414 123 620 1135 681 191 Cuts at: 5470 826 Size: 1471 Fragments arranged by size: 1471 1261 1135 681 620 414 219 191 123 SfoI GGC'GCC Cuts at: 2209 2209 Size: 6115 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6115 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6115 SmaI CCC'GGG Cuts at: 1872 2092 2402 3442 1872 Size: 220 310 1040 4545 Fragments arranged by size: 4545 1040 310 220 SmlI C'TyrA_G Cuts at: 820 1017 1943 2540 2687 3086 4219 5120 5382 Size: 197 926 597 147 399 1133 901 262 Cuts at: 5382 820 Size: 1553 Fragments arranged by size: 1553 1133 926 901 597 399 262 197 147 SnaBI TAC'GTA Cuts at: 493 3906 493 Size: 3413 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6115 SphI G_CATG'C Cuts at: 1132 3473 1132 Size: 2341 3774 SspI AAT'ATT Cuts at: 5 52 4039 5 Size: 47 3987 2081 Fragments arranged by size: 3987 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6115 StyI C'CwwG_G Cuts at: 513 1065 2247 3554 513 Size: 552 1182 1307 3074 Fragments arranged by size: 3074 1307 1182 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2057 4142 4159 4312 4497 4942 325 Size: 1255 477 2085 17 153 185 445 1498 Fragments arranged by size: 2085 1498 1255 477 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4361 6073 96 Size: 274 80 33 51 1443 2384 1712 138 Fragments arranged by size: 2384 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6075 6107 6075 Size: 32 6083 Tth111I GACn'n_nGTC Cuts at: 1316 3361 1316 Size: 2045 4070 XbaI T'CTAG_A Cuts at: 3451 3451 Size: 6115 XhoI C'TCGA_G Cuts at: 1943 3086 1943 Size: 1143 4972 XmaI C'CCGG_G Cuts at: 1870 2090 2400 3440 1870 Size: 220 310 1040 4545 Fragments arranged by size: 4545 1040 310 220 XmnI GAAnn'nnTTC Cuts at: 4244 4244 Size: 6115 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3923 276 Size: 53 83 186 936 2389 2468 Fragments arranged by size: 2468 2389 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI