Restriction map for
pFN21AE6340
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BcnI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrFI BsrGI
BssHII BssKI BssSI Bst1107I BstAPI
BstBI BstEII BstNI BstPI BstUI
BstXI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRII EcoRV EcoT22I FauI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KspI MaeII
MaeIII MamI MfeI MluI MroI
MspI MunI MvaI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI ScaI ScrFI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tsp45I
Tth111I Van91I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 405 GGTACC G^GTACC
AlwNI 44 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 609 CCTGAGG CC^T[ACGT]AGG
Asp700I 250 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 405 GGTACC G^GTACC
AspHI 842 GTGCTC G[AT]GC[AT]^C
BalI 596 TGGCCA TGG^CCA
BanI 405 GGTACC G^G[CT][GA]CC
BanII 344 GAGCCC G[GA]GC[CT]^C
BclI 867 TGATCA T^GATCA
BglII 795 AGATCT A^GATCT
BpmI 493 CTGGAG CTGGAG(16/14)
BsaJI 128 CCATGG C^C[ACGT][ACGT]GG
BseRI 400 GAGGAG GAGGAG(10/8)
BsiHKAI 842 GTGCTC G[AT]GC[AT]^C
BspHI 111 TCATGA T^CATGA
BsrDI 416 CATTGC GCAATG(2/0)
BsrI 149 CCAGT ACTGG(1/-1)
BstYI 795 AGATCT [GA]^GATC[CT]
Bsu36I 609 CCTGAGG CC^T[ACGT]AGG
DraI 562 TTTAAA TTT^AAA
DraII 478 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 48 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 128 CCATGG C^C[GA][CT]GG
EaeI 596 TGGCCA [CT]^GGCC[GA]
EarI 370 CTCTTC CTCTTC(1/4)
Eco81I 609 CCTGAGG CC^T[ACGT]AGG
EcoO109I 478 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 467 GAATTC G^AATTC
EcoT14I 128 CCATGG C^C[AT][AT]GG
FbaI 867 TGATCA T^GATCA
HphI 861 TCACC GGTGA(8/7)
KpnI 405 GGTACC GGTAC^C
Ksp632I 370 CTCTTC CTCTTC(1/4)
MflI 795 AGATCT [GA]^GATC[CT]
MluNI 596 TGGCCA TGG^CCA
MscI 596 TGGCCA TGG^CCA
MslI 24 CATGTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 355 CAGCTG C[AC]G^C[GT]G
MwoI 598 GCCAAGAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 128 CCATGG C^CATGG
PleI 569 GACTC GAGTC(4/5)
PpuMI 478 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 355 CAGCTG CAG^CTG
RcaI 111 TCATGA T^CATGA
SapI 369 GCTCTTC GCTCTTC(1/4)
StyI 128 CCATGG C^C[AT][AT]GG
TaqI 119 TCGA T^CGA
TthHB8I 119 TCGA T^CGA
XhoII 795 AGATCT [GA]^GATC[CT]
XmnI 250 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 85 GTAC GT^AC
406 GTAC
AvaII 479 GGACC G^G[AT]CC
955 GGACC
BmyI 344 GAGCCC G[AGT]GC[ACT]^C
842 GTGCTC
BsaI 184 GGTCTC GGTCTC(1/5)
757 GGTCTC
BsiYI 763 CCATCAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
972 CCTCCGTCTGG
BslI 763 CCATCAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
972 CCTCCGTCTGG
BsmI 276 GAATGC GAATGC(1/-1)
603 GAATGC
Bsp1286I 344 GAGCCC G[AGT]GC[ACT]^C
842 GTGCTC
BspMI 1024 ACCTGC ACCTGC(4/8)
72 GCAGGT
BstF5I 917 CATCC GGATG(2/0)
981 GGATG
Cfr13I 479 GGACC G^G[ACGT]CC
955 GGACC
DpnII 796 GATC ^GATC
868 GATC
FokI 917 CATCC GGATG(9/13)
981 GGATG
HaeIII 597 GGCC GG^CC
717 GGCC
MboI 796 GATC ^GATC
868 GATC
NdeII 796 GATC ^GATC
868 GATC
RsaI 85 GTAC GT^AC
406 GTAC
Sau3AI 796 GATC ^GATC
868 GATC
Sau96I 479 GGACC G^G[ACGT]CC
955 GGACC
SmlI 473 CTCAAG C^T[CT][GA]AG
571 CTCAAG
TspRI 48 CACTG CA[GC]TG[ACGT][ACGT]^
840 CAGTG
XbaI 58 TCTAGA T^CTAGA
792 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AcsI AluI ApoI BbvI BfaI
BsmAI BsmFI Cac8I DdeI Fnu4HI
HinfI ItaI MaeI MboII MnlI
MseI NlaIII NlaIV PstI SfcI
TfiI Tru9I TseI Tsp509I