(Circular) (Six-base) MAPSORT of: pFN21AE6340.seq Check: 6570 from: 1 to: 5761 pFN21AE6340 5761 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3571 278 Size: 53 83 186 936 2035 2468 Fragments arranged by size: 2468 2035 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3104 1937 Size: 1167 4594 Acc65I G'GTAC_C Cuts at: 2410 3082 2410 Size: 672 5089 AclI AA'CG_TT Cuts at: 1563 3888 4261 1563 Size: 2325 373 3063 Fragments arranged by size: 3063 2325 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2384 2935 3019 3824 5208 5476 1632 Size: 752 551 84 805 1384 268 1917 Fragments arranged by size: 1917 1384 805 752 551 268 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5564 AflIII A'CryG_T Cuts at: 1203 4660 5637 1203 Size: 3457 977 1327 Fragments arranged by size: 3457 1327 977 AgeI A'CCGG_T Cuts at: 4592 4592 Size: 5761 AhdI GACnn_n'nnGTC Cuts at: 4490 4490 Size: 5761 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5761 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5761 AlwNI CAG_nnn'CTG Cuts at: 2054 3504 5076 2054 Size: 1450 1572 2739 Fragments arranged by size: 2739 1572 1450 ApaLI G'TGCA_C Cuts at: 3818 4974 3818 Size: 1156 4605 ApoI r'AATT_y Cuts at: 1493 2199 2220 2472 3070 3296 3448 5557 1493 Size: 706 21 252 598 226 152 2109 1697 Fragments arranged by size: 2109 1697 706 598 252 226 152 21 AseI AT'TA_AT Cuts at: 160 1032 4315 160 Size: 872 3283 1606 Fragments arranged by size: 3283 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 AvaI C'yCGr_G Cuts at: 1870 1943 3086 1870 Size: 73 1143 4545 Fragments arranged by size: 4545 1143 73 BamHI G'GATC_C Cuts at: 3091 3458 3091 Size: 367 5394 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2410 3082 4537 618 Size: 325 217 627 623 672 1455 1842 Fragments arranged by size: 1842 1455 672 627 623 325 217 BanII G_rGCy'C Cuts at: 1391 2353 3080 1391 Size: 962 727 4072 Fragments arranged by size: 4072 962 727 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4790 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3952 3986 1903 Size: 34 2015 34 3678 Fragments arranged by size: 3678 2015 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3653 4863 3653 Size: 1210 4551 BclI T'GATC_A Cuts at: 1548 2872 1548 Size: 1324 4437 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4372 136 Size: 107 122 71 631 605 2700 1525 Fragments arranged by size: 2700 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2800 5756 2800 Size: 2956 2805 BlpI GC'TnA_GC Cuts at: 3178 3178 Size: 5761 Bme1580I G_kGCm'C Cuts at: 1790 3822 4978 1790 Size: 2032 1156 2573 Fragments arranged by size: 2573 2032 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5761 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2148 3492 4450 Size: 416 53 83 399 24 731 1344 958 Cuts at: 4450 442 Size: 1753 Fragments arranged by size: 1753 1344 958 731 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5761 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2519 4421 1239 Size: 1280 1902 2579 Fragments arranged by size: 2579 1902 1280 Bpu10I CC'TnA_GC Cuts at: 2354 2354 Size: 5761 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2463 2561 3886 4751 5049 2463 Size: 98 1325 865 298 3175 Fragments arranged by size: 3175 1325 865 298 98 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2195 2768 4424 882 Size: 617 696 573 1656 2219 Fragments arranged by size: 2219 1656 696 617 573 BsaAI yAC'GTr Cuts at: 493 1204 3552 493 Size: 711 2348 2702 Fragments arranged by size: 2702 2348 711 BsaBI GATnn'nnATC Cuts at: 5755 5755 Size: 5761 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3568 3950 4585 275 Size: 53 83 186 936 2035 382 635 1451 Fragments arranged by size: 2035 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4194 4592 4866 5013 5424 4194 Size: 398 274 147 411 4531 Fragments arranged by size: 4531 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5536 Fragments arranged by size: 5536 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2420 1186 Size: 1234 4527 BseYI C'CCAG_C Cuts at: 2289 2915 4964 5460 2289 Size: 626 2049 496 2590 Fragments arranged by size: 2590 2049 626 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5188 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3211 3972 5000 1540 Size: 275 186 1210 761 1028 2301 Fragments arranged by size: 2301 1210 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2851 3822 3907 4978 2851 Size: 971 85 1071 3634 Fragments arranged by size: 3634 1071 971 85 BsmI GAATG_Cn' Cuts at: 2287 2614 3272 3365 2287 Size: 327 658 93 4683 Fragments arranged by size: 4683 658 327 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2353 2851 3080 3822 3907 4978 1391 Size: 399 563 498 229 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 563 498 399 229 85 BspEI T'CCGG_A Cuts at: 5424 5424 Size: 5761 BspHI T'CATG_A Cuts at: 2116 3650 5380 2116 Size: 1534 1730 2497 Fragments arranged by size: 2497 1730 1534 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2068 3038 3116 844 Size: 1224 970 78 3489 Fragments arranged by size: 3489 1224 970 78 BsrBI CCG'CTC Cuts at: 1394 1823 3648 1394 Size: 429 1825 3507 Fragments arranged by size: 3507 1825 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2420 4256 4430 65 Size: 1183 1172 1836 174 1396 Fragments arranged by size: 1836 1396 1183 1172 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4405 4592 1700 Size: 110 2595 187 2869 Fragments arranged by size: 2869 2595 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5761 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5761 BssSI C'ACGA_G Cuts at: 3821 4833 3821 Size: 1012 4749 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5761 BstBI TT'CG_AA Cuts at: 4565 4565 Size: 5761 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5761 BstYI r'GATC_y Cuts at: 1972 2800 3091 3458 3843 3860 5312 5398 5410 Size: 828 291 367 385 17 1452 86 12 Cuts at: 5410 5756 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 828 385 367 346 291 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2615 2615 Size: 5761 BtgI C'CryG_G Cuts at: 513 1065 2004 2133 513 Size: 552 939 129 4141 Fragments arranged by size: 4141 939 552 129 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5022 Fragments arranged by size: 5022 683 56 BtsI GCAGTG_nn' Cuts at: 3277 4071 4091 3277 Size: 794 20 4947 Fragments arranged by size: 4947 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5761 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5726 DraI TTT'AAA Cuts at: 2569 3065 2569 Size: 496 5265 DraIII CAC_nnn'GTG Cuts at: 2058 2058 Size: 5761 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4768 809 Size: 507 3452 1802 Fragments arranged by size: 3452 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2601 3208 4097 Size: 54 1425 50 275 18 771 607 889 Cuts at: 4097 5726 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 771 607 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3208 1537 Size: 275 1396 4090 Fragments arranged by size: 4090 1396 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2381 3691 1353 Size: 1028 1310 3423 Fragments arranged by size: 3423 1310 1028 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4348 4722 4868 387 Size: 1064 138 2759 374 146 1280 Fragments arranged by size: 2759 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2384 2519 2935 3019 3824 4421 5208 Size: 393 752 135 416 84 805 597 787 Cuts at: 5208 5476 1239 Size: 268 1524 Fragments arranged by size: 1524 805 787 752 597 416 393 268 135 84 EcoO109I rG'GnC_Cy Cuts at: 2484 2484 Size: 5761 EcoRI G'AATT_C Cuts at: 2472 3070 3448 2472 Size: 598 378 4785 Fragments arranged by size: 4785 598 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2392 2580 2392 Size: 188 5573 FspI TGC'GCA Cuts at: 4267 4267 Size: 5761 HaeII r_GCGC'y Cuts at: 1678 4908 1678 Size: 3230 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2722 2754 2774 2806 4402 Size: 32 135 32 1150 32 20 32 1596 Cuts at: 4402 4434 4476 4508 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1596 1150 135 42 32 32 32 32 32 32 20 HincII GTy'rAC Cuts at: 669 1657 1938 3105 3351 669 Size: 988 281 1167 246 3079 Fragments arranged by size: 3079 1167 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5761 HpaI GTT'AAC Cuts at: 3351 3351 Size: 5761 KpnI G_GTAC'C Cuts at: 2414 3086 2414 Size: 672 5089 MfeI C'AATT_G Cuts at: 3360 3360 Size: 5761 MluI A'CGCG_T Cuts at: 5637 5637 Size: 5761 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4875 5059 1305 Size: 3570 184 2007 Fragments arranged by size: 3570 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2603 10 Size: 54 1425 343 771 3168 Fragments arranged by size: 3168 1425 771 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2033 3719 4078 4237 518 Size: 648 867 1686 359 159 2042 Fragments arranged by size: 2042 1686 867 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2362 3177 3854 5002 5247 1606 Size: 756 815 677 1148 245 2120 Fragments arranged by size: 2120 1148 815 756 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2133 513 Size: 552 939 129 4141 Fragments arranged by size: 4141 939 552 129 NdeI CA'TA_TG Cuts at: 387 387 Size: 5761 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5761 NotI GC'GGCC_GC Cuts at: 3208 3208 Size: 5761 NspI r_CATG'y Cuts at: 1132 1229 3119 4664 1132 Size: 97 1890 1545 2229 Fragments arranged by size: 2229 1890 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5761 PciI A'CATG_T Cuts at: 4660 4660 Size: 5761 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5761 PmeI GTTT'AAAC Cuts at: 3065 3065 Size: 5761 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3830 3862 5343 5375 3830 Size: 32 1481 32 4216 Fragments arranged by size: 4216 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2484 2484 Size: 5761 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5761 PsiI TTA'TAA Cuts at: 3331 3331 Size: 5761 PstI C_TGCA'G Cuts at: 830 2859 2938 3048 3113 830 Size: 2029 79 110 65 3478 Fragments arranged by size: 3478 2029 110 79 65 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5761 PvuII CAG'CTG Cuts at: 2362 2362 Size: 5761 SalI G'TCGA_C Cuts at: 1936 3103 1936 Size: 1167 4594 SapI GCTCTTCn'nnn_ Cuts at: 2381 2381 Size: 5761 SbfI CC_TGCA'GG Cuts at: 3113 3113 Size: 5761 ScaI AGT'ACT Cuts at: 4009 5721 4009 Size: 1712 4049 SfcI C'TryA_G Cuts at: 826 1045 2522 2855 2934 3044 3109 4244 4925 Size: 219 1477 333 79 110 65 1135 681 Cuts at: 4925 5116 826 Size: 191 1471 Fragments arranged by size: 1477 1471 1135 681 333 219 191 110 79 65 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5761 SmaI CCC'GGG Cuts at: 1872 3088 1872 Size: 1216 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2478 2576 3865 4766 5028 820 Size: 197 926 535 98 1289 901 262 1553 Fragments arranged by size: 1553 1289 926 901 535 262 197 98 SnaBI TAC'GTA Cuts at: 493 3552 493 Size: 3059 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5761 SphI G_CATG'C Cuts at: 1132 3119 1132 Size: 1987 3774 SspI AAT'ATT Cuts at: 5 52 3685 5 Size: 47 3633 2081 Fragments arranged by size: 3633 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5761 StyI C'CwwG_G Cuts at: 513 1065 2004 2133 3200 513 Size: 552 939 129 1067 3074 Fragments arranged by size: 3074 1067 939 552 129 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2023 2167 2303 3788 3805 3958 4143 Size: 1255 443 144 136 1485 17 153 185 Cuts at: 4143 4588 325 Size: 445 1498 Fragments arranged by size: 1498 1485 1255 445 443 185 153 144 136 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2089 4007 5719 Size: 274 80 33 51 1443 112 1918 1712 Cuts at: 5719 96 Size: 138 Fragments arranged by size: 1918 1712 1443 274 138 112 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2818 2850 5721 5753 2818 Size: 32 2871 32 2826 Fragments arranged by size: 2871 2826 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5761 XbaI T'CTAG_A Cuts at: 2063 2797 3097 2063 Size: 734 300 4727 Fragments arranged by size: 4727 734 300 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5761 XmaI C'CCGG_G Cuts at: 1870 3086 1870 Size: 1216 4545 XmnI GAAnn'nnTTC Cuts at: 2259 3890 2259 Size: 1631 4130 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3569 276 Size: 53 83 186 936 2035 2468 Fragments arranged by size: 2468 2035 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI