(Linear) (Six-base) MAPSORT of: KIEE6340.seq Check: 7180 from: 1 to: 1056 KIEE6340.orf With 182 enzymes: SgfI * March 16, 2015 11:30 .. Acc65I G'GTAC_C Cuts at: 0 405 1056 Size: 405 651 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 379 930 1014 1056 Size: 379 551 84 42 Fragments arranged by size: 551 379 84 42 AlwNI CAG_nnn'CTG Cuts at: 0 49 1056 Size: 49 1007 ApoI r'AATT_y Cuts at: 0 194 215 467 1056 Size: 194 21 252 589 Fragments arranged by size: 589 252 194 21 BanI G'GyrC_C Cuts at: 0 405 1056 Size: 405 651 BanII G_rGCy'C Cuts at: 0 348 1056 Size: 348 708 BclI T'GATC_A Cuts at: 0 867 1056 Size: 867 189 BglII A'GATC_T Cuts at: 0 795 1056 Size: 795 261 BmrI ACTGGGnnnn_n' Cuts at: 0 143 1056 Size: 143 913 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 514 1056 Size: 514 542 Bpu10I CC'TnA_GC Cuts at: 0 349 1056 Size: 349 707 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 458 556 1056 Size: 458 98 500 Fragments arranged by size: 500 458 98 BsaI GGTCTCn'nnnn_ Cuts at: 0 190 763 1056 Size: 190 573 293 Fragments arranged by size: 573 293 190 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 415 1056 Size: 415 641 BseYI C'CCAG_C Cuts at: 0 284 910 1056 Size: 284 626 146 Fragments arranged by size: 626 284 146 BsiHKAI G_wGCw'C Cuts at: 0 846 1056 Size: 846 210 BsmI GAATG_Cn' Cuts at: 0 282 609 1056 Size: 282 327 447 Fragments arranged by size: 447 327 282 Bsp1286I G_dGCh'C Cuts at: 0 348 846 1056 Size: 348 498 210 Fragments arranged by size: 498 348 210 BspHI T'CATG_A Cuts at: 0 111 1056 Size: 111 945 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 63 1033 1056 Size: 63 970 23 Fragments arranged by size: 970 63 23 BsrDI GCAATG_nn' Cuts at: 0 415 1056 Size: 415 641 BstYI r'GATC_y Cuts at: 0 795 1056 Size: 795 261 Bsu36I CC'TnA_GG Cuts at: 0 610 1056 Size: 610 446 BtgI C'CryG_G Cuts at: 0 128 1056 Size: 128 928 DraI TTT'AAA Cuts at: 0 564 1056 Size: 564 492 DraIII CAC_nnn'GTG Cuts at: 0 53 1056 Size: 53 1003 EaeI y'GGCC_r Cuts at: 0 596 1056 Size: 596 460 EarI CTCTTCn'nnn_ Cuts at: 0 376 1056 Size: 376 680 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 379 514 930 1014 1056 Size: 379 135 416 84 42 Fragments arranged by size: 416 379 135 84 42 EcoO109I rG'GnC_Cy Cuts at: 0 479 1056 Size: 479 577 EcoRI G'AATT_C Cuts at: 0 467 1056 Size: 467 589 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 387 575 1056 Size: 387 188 481 Fragments arranged by size: 481 387 188 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 717 749 769 801 1056 Size: 717 32 20 32 255 Fragments arranged by size: 717 255 32 32 20 KpnI G_GTAC'C Cuts at: 0 409 1056 Size: 409 647 MscI TGG'CCA Cuts at: 0 598 1056 Size: 598 458 MslI CAynn'nnrTG Cuts at: 0 28 1056 Size: 28 1028 MspA1I CmG'CkG Cuts at: 0 357 1056 Size: 357 699 NcoI C'CATG_G Cuts at: 0 128 1056 Size: 128 928 PpuMI rG'GwC_Cy Cuts at: 0 479 1056 Size: 479 577 PstI C_TGCA'G Cuts at: 0 854 933 1043 1056 Size: 854 79 110 13 Fragments arranged by size: 854 110 79 13 PvuII CAG'CTG Cuts at: 0 357 1056 Size: 357 699 SapI GCTCTTCn'nnn_ Cuts at: 0 376 1056 Size: 376 680 SfcI C'TryA_G Cuts at: 0 517 850 929 1039 1056 Size: 517 333 79 110 17 Fragments arranged by size: 517 333 110 79 17 SmlI C'TyrA_G Cuts at: 0 473 571 1056 Size: 473 98 485 Fragments arranged by size: 485 473 98 StyI C'CwwG_G Cuts at: 0 128 1056 Size: 128 928 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 18 162 298 1056 Size: 18 144 136 758 Fragments arranged by size: 758 144 136 18 TatI w'GTAC_w Cuts at: 0 84 1056 Size: 84 972 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 813 845 1056 Size: 813 32 211 Fragments arranged by size: 813 211 32 XbaI T'CTAG_A Cuts at: 0 58 792 1056 Size: 58 734 264 Fragments arranged by size: 734 264 58 XmnI GAAnn'nnTTC Cuts at: 0 254 1056 Size: 254 802 Enzymes that do cut: Acc65I AcuI AlwNI ApoI BanI BanII BclI BglII BmrI BpmI Bpu10I BpuEI BsaI BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BstYI Bsu36I BtgI DraI DraIII EaeI EarI Eco57MI EcoO109I EcoRI FalI Hin4I KpnI MscI MslI MspA1I NcoI PpuMI PstI PvuII SapI SfcI SmlI StyI TaqII TatI TstI XbaI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BfrBI BglI BlpI Bme1580I BmgBI BmtI BplI BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI DrdI EagI EciI EcoICRI EcoNI EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XcmI XhoI XmaI ZraI