################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccIII AcsI AcyI AflII AflIII AgeI AhdI AlwNI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BanI BanII BbeI BbrPI BcgI BclI BfrI BglI BlnI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoT22I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI TfiI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AccI 817 GTCTAC GT^[AC][GT]AC AccII 879 CGCG CG^CG AciI 950 CCGC CCGC(-3/-1) AclI 584 AACGTT AA^CGTT Alw44I 936 GTGCAC G^TGCAC AocI 783 CCTGAGG CC^T[ACGT]AGG ApaLI 936 GTGCAC G^TGCAC AspHI 936 GTGCAC G[AT]GC[AT]^C AvaI 146 CCCGAG C^[CT]CG[GA]G AvaII 138 GGACC G^G[AT]CC BbsI 75 GTCTTC GAAGAC(2/6) BbvCI 918 CCTCAGC CCTCAGC(-5/-2) BciVI 576 GGATAC GTATCC(6/5) BcnI 873 CCCGG CC^[GC]GG BfaI 167 CTAG C^TAG BglII 1246 AGATCT A^GATCT BpmI 601 CTGGAG CTGGAG(16/14) BpuAI 75 GTCTTC GAAGAC(2/6) BsaI 1204 GAGACC GGTCTC(1/5) BsiHKAI 936 GTGCAC G[AT]GC[AT]^C BsmFI 1168 GGGAC GGGAC(10/14) BsmI 230 GCATTC GAATGC(1/-1) BsoBI 146 CCCGAG C^[CT]CG[GA]G BspHI 459 TCATGA T^CATGA BstF5I 480 CATCC GGATG(2/0) BstUI 879 CGCG CG^CG Bsu36I 783 CCTGAGG CC^T[ACGT]AGG Cfr13I 138 GGACC G^G[ACGT]CC DraII 137 AGGACCT [GA]G^G[ACGT]CC[CT] DsaI 572 CCATGG C^C[GA][CT]GG Eco81I 783 CCTGAGG CC^T[ACGT]AGG EcoO109I 137 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRV 694 GATATC GAT^ATC EcoT14I 572 CCATGG C^C[AT][AT]GG FokI 480 CATCC GGATG(9/13) HaeIII 927 GGCC GG^CC HapII 874 CCGG C^CGG HgaI 877 GACGC GACGC(5/10) HindIII 1272 AAGCTT A^AGCTT HinfI 1170 GACTC G^A[ACGT]TC HpaII 874 CCGG C^CGG MaeI 167 CTAG C^TAG MaeII 585 ACGT A^CGT MslI 759 CACATGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 950 CCGCTG C[AC]G^C[GT]G MspI 874 CCGG C^CGG MvnI 879 CGCG CG^CG NciI 873 CCCGG CC^[GC]GG NcoI 572 CCATGG C^CATGG NspI 760 ACATGC [GA]CATG^[CT] PleI 1170 GACTC GAGTC(4/5) PpuMI 137 AGGACCT [GA]G^G[AT]CC[CT] Psp1406I 584 AACGTT AA^CGTT RcaI 459 TCATGA T^CATGA Sau96I 138 GGACC G^G[ACGT]CC SfcI 300 CTATAG C^T[GA][CT]AG StyI 572 CCATGG C^C[AT][AT]GG Tsp45I 466 GTGAC ^GT[GC]AC XcmI 890 CCAGAGTGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### BamHI 274 GGATCC G^GATCC 390 GGATCC BbvI 1405 GCAGC GCAGC(8/12) 886 GCTGC BlpI 952 GCTGAGC GC^T[ACGT]AGC 1108 GCTGAGC BmyI 895 GTGCCC G[AGT]GC[ACT]^C 936 GTGCAC Bpu1102I 952 GCTGAGC GC^T[ACGT]AGC 1108 GCTGAGC BseRI 484 CTCCTC GAGGAG(10/8) 786 GAGGAG BsmAI 321 GAGAC GTCTC(1/5) 1204 GAGAC Bsp1286I 895 GTGCCC G[AGT]GC[ACT]^C 936 GTGCAC Bsp1407I 401 TGTACA T^GTACA 727 TGTACA BsrGI 401 TGTACA T^GTACA 727 TGTACA BsrI 600 ACTGG ACTGG(1/-1) 356 CCAGT Cac8I 1003 GCTTGC GC[ACGT]^[ACGT]GC 1113 GCAAGC CelII 952 GCTGAGC GC^T[ACGT]AGC 1108 GCTGAGC HpaI 307 GTTAAC GTT^AAC 1330 GTTAAC NlaIV 274 GGATCC GG[ACGT]^[ACGT]CC 390 GGATCC SspBI 401 TGTACA T^GTACA 727 TGTACA TseI 886 GCTGC G^C[AT]GC 1405 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AfaI AluI AlwI BsaJI BsiYI BslI BspMI BssKI BstNI BstYI DdeI DpnII EcoRII Fnu4HI HincII HindII HphI ItaI MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII RsaI Sau3AI ScrFI SfaNI Tru9I Tsp509I TspRI XhoII