(Circular) (Six-base) MAPSORT of: pFN21AE1438.seq Check: 1324 from: 1 to: 5590 pFN21AE1438 5590 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2671 2671 Size: 5590 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2619 3400 278 Size: 53 83 186 936 1083 781 2468 Fragments arranged by size: 2468 1083 936 781 186 83 53 AccI GT'mk_AC Cuts at: 1937 2933 1937 Size: 996 4594 Acc65I G'GTAC_C Cuts at: 2911 2911 Size: 5590 AclI AA'CG_TT Cuts at: 1563 3717 4090 1563 Size: 2154 373 3063 Fragments arranged by size: 3063 2154 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2227 2300 3653 5037 5305 1632 Size: 595 73 1353 1384 268 1917 Fragments arranged by size: 1917 1384 1353 595 268 73 AfeI AGC'GCT Cuts at: 2300 2300 Size: 5590 AflII C'TTAA_G Cuts at: 820 1017 2291 820 Size: 197 1274 4119 Fragments arranged by size: 4119 1274 197 AflIII A'CryG_T Cuts at: 1203 4489 5466 1203 Size: 3286 977 1327 Fragments arranged by size: 3286 1327 977 AgeI A'CCGG_T Cuts at: 4421 4421 Size: 5590 AhdI GACnn_n'nnGTC Cuts at: 4319 4319 Size: 5590 AleI CACnn'nnGTG Cuts at: 1166 2210 1166 Size: 1044 4546 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5590 AlwNI CAG_nnn'CTG Cuts at: 3333 4905 3333 Size: 1572 4018 ApaLI G'TGCA_C Cuts at: 2568 3647 4803 2568 Size: 1079 1156 3355 Fragments arranged by size: 3355 1156 1079 ApoI r'AATT_y Cuts at: 1493 2899 3125 3277 5386 1493 Size: 1406 226 152 2109 1697 Fragments arranged by size: 2109 1697 1406 226 152 AseI AT'TA_AT Cuts at: 160 1032 4144 160 Size: 872 3112 1606 Fragments arranged by size: 3112 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5590 AvaI C'yCGr_G Cuts at: 1870 1943 2915 1870 Size: 73 972 4545 Fragments arranged by size: 4545 972 73 BamHI G'GATC_C Cuts at: 2920 3287 2920 Size: 367 5223 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2683 2911 4366 618 Size: 325 217 627 896 228 1455 1842 Fragments arranged by size: 1842 1455 896 627 325 228 217 BanII G_rGCy'C Cuts at: 1391 2058 2322 2909 1391 Size: 667 264 587 4072 Fragments arranged by size: 4072 667 587 264 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4619 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2485 2519 3781 3815 1903 Size: 34 548 34 1262 34 3678 Fragments arranged by size: 3678 1262 548 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3482 4692 3482 Size: 1210 4380 BclI T'GATC_A Cuts at: 1548 2530 1548 Size: 982 4608 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2172 2703 4201 Size: 107 122 71 631 605 500 531 1498 Cuts at: 4201 136 Size: 1525 Fragments arranged by size: 1525 1498 631 605 531 500 122 107 71 BglII A'GATC_T Cuts at: 5585 5585 Size: 5590 BlpI GC'TnA_GC Cuts at: 3007 3007 Size: 5590 Bme1580I G_kGCm'C Cuts at: 1790 2382 2572 3651 4807 1790 Size: 592 190 1079 1156 2573 Fragments arranged by size: 2573 1156 1079 592 190 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5590 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2217 2377 3321 Size: 416 53 83 399 24 800 160 944 Cuts at: 3321 4279 442 Size: 958 1753 Fragments arranged by size: 1753 958 944 800 416 399 160 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5590 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4250 1239 Size: 3011 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2737 2861 3715 4580 4878 2737 Size: 124 854 865 298 3449 Fragments arranged by size: 3449 865 854 298 124 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4253 882 Size: 617 2754 2219 Fragments arranged by size: 2754 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2872 3381 493 Size: 711 1668 509 2702 Fragments arranged by size: 2702 1668 711 509 BsaBI GATnn'nnATC Cuts at: 5584 5584 Size: 5590 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2616 3397 3779 4414 Size: 53 83 186 936 1083 781 382 635 Cuts at: 4414 275 Size: 1451 Fragments arranged by size: 1451 1083 936 781 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2022 4023 4421 4695 4842 5253 2022 Size: 2001 398 274 147 411 2359 Fragments arranged by size: 2359 2001 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5365 Fragments arranged by size: 5365 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5590 BseYI C'CCAG_C Cuts at: 4793 5289 4793 Size: 496 5094 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2699 1163 Size: 573 963 4054 Fragments arranged by size: 4054 963 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2049 3040 3801 4829 1540 Size: 275 186 48 991 761 1028 2301 Fragments arranged by size: 2301 1028 991 761 275 186 48 BsiHKAI G_wGCw'C Cuts at: 2190 2472 2572 2877 3651 3736 4807 2190 Size: 282 100 305 774 85 1071 2973 Fragments arranged by size: 2973 1071 774 305 282 100 85 BsmI GAATG_Cn' Cuts at: 3101 3194 3101 Size: 93 5497 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2058 2190 2322 2382 2472 2572 2877 Size: 399 268 132 132 60 90 100 305 Cuts at: 2877 2909 3651 3736 4807 1391 Size: 32 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 305 268 132 132 100 90 85 60 32 BspEI T'CCGG_A Cuts at: 5253 5253 Size: 5590 BspHI T'CATG_A Cuts at: 3479 5209 3479 Size: 1730 3860 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2671 2945 844 Size: 1827 274 3489 Fragments arranged by size: 3489 1827 274 BsrBI CCG'CTC Cuts at: 1394 1823 2031 3477 1394 Size: 429 208 1446 3507 Fragments arranged by size: 3507 1446 429 208 BsrDI GCAATG_nn' Cuts at: 65 1248 2490 4085 4259 65 Size: 1183 1242 1595 174 1396 Fragments arranged by size: 1595 1396 1242 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2307 2472 4234 4421 1700 Size: 110 497 165 1762 187 2869 Fragments arranged by size: 2869 1762 497 187 165 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5590 BssHII G'CGCG_C Cuts at: 1925 2087 1925 Size: 162 5428 BssSI C'ACGA_G Cuts at: 2315 3650 4662 2315 Size: 1335 1012 3243 Fragments arranged by size: 3243 1335 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5590 BstBI TT'CG_AA Cuts at: 4394 4394 Size: 5590 BstEII G'GTnAC_C Cuts at: 1184 2659 1184 Size: 1475 4115 BstXI CCAn_nnnn'nTGG Cuts at: 2227 2227 Size: 5590 BstYI r'GATC_y Cuts at: 1972 2920 3287 3672 3689 5141 5227 5239 5585 Size: 948 367 385 17 1452 86 12 346 Cuts at: 5585 1972 Size: 1977 Fragments arranged by size: 1977 1452 948 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4099 Fragments arranged by size: 4099 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2519 487 Size: 683 56 1293 3558 Fragments arranged by size: 3558 1293 683 56 BtsI GCAGTG_nn' Cuts at: 3106 3900 3920 3106 Size: 794 20 4776 Fragments arranged by size: 4776 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5590 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5555 DraI TTT'AAA Cuts at: 2894 2894 Size: 5590 DraIII CAC_nnn'GTG Cuts at: 2659 2659 Size: 5590 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2336 4597 809 Size: 507 1020 2261 1802 Fragments arranged by size: 2261 1802 1020 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2695 3037 Size: 54 1425 50 275 18 216 649 342 Cuts at: 3037 3926 5555 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 649 342 275 216 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2046 3037 1537 Size: 275 234 991 4090 Fragments arranged by size: 4090 991 275 234 EarI CTCTTCn'nnn_ Cuts at: 1353 2229 3520 1353 Size: 876 1291 3423 Fragments arranged by size: 3423 1291 876 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4177 4551 4697 387 Size: 1064 138 2588 374 146 1280 Fragments arranged by size: 2588 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2227 2300 3653 4250 5037 5305 1239 Size: 393 595 73 1353 597 787 268 1524 Fragments arranged by size: 1524 1353 787 597 595 393 268 73 EcoO109I rG'GnC_Cy Cuts at: 2515 2515 Size: 5590 EcoRI G'AATT_C Cuts at: 2899 3277 2899 Size: 378 5212 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2620 2620 Size: 5590 FspI TGC'GCA Cuts at: 4096 4096 Size: 5590 HaeII r_GCGC'y Cuts at: 1678 2302 2483 4737 1678 Size: 624 181 2254 2531 Fragments arranged by size: 2531 2254 624 181 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4231 4263 4305 4337 1373 Size: 32 135 32 2659 32 42 32 2626 Fragments arranged by size: 2659 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2934 3180 669 Size: 988 281 996 246 3079 Fragments arranged by size: 3079 996 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5590 HpaI GTT'AAC Cuts at: 3180 3180 Size: 5590 KpnI G_GTAC'C Cuts at: 2915 2915 Size: 5590 MfeI C'AATT_G Cuts at: 3189 3189 Size: 5590 MluI A'CGCG_T Cuts at: 5466 5466 Size: 5590 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4704 4888 1305 Size: 3399 184 2007 Fragments arranged by size: 3399 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2697 10 Size: 54 1425 343 865 2903 Fragments arranged by size: 2903 1425 865 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2210 2870 3548 3907 4066 518 Size: 648 1044 660 678 359 159 2042 Fragments arranged by size: 2042 1044 678 660 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2101 2182 3006 3683 4831 5076 1606 Size: 495 81 824 677 1148 245 2120 Fragments arranged by size: 2120 1148 824 677 495 245 81 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4099 Fragments arranged by size: 4099 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5590 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5590 NotI GC'GGCC_GC Cuts at: 3037 3037 Size: 5590 NspI r_CATG'y Cuts at: 1132 1229 2948 4493 1132 Size: 97 1719 1545 2229 Fragments arranged by size: 2229 1719 1545 97 PasI CC'CwG_GG Cuts at: 1348 2035 2576 1348 Size: 687 541 4362 Fragments arranged by size: 4362 687 541 PciI A'CATG_T Cuts at: 4489 4489 Size: 5590 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5590 PfoI T'CCnGG_A Cuts at: 2058 2058 Size: 5590 PmeI GTTT'AAAC Cuts at: 2894 2894 Size: 5590 PmlI CAC'GTG Cuts at: 2872 2872 Size: 5590 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3659 3691 5172 5204 3659 Size: 32 1481 32 4045 Fragments arranged by size: 4045 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5590 PsiI TTA'TAA Cuts at: 3160 3160 Size: 5590 PstI C_TGCA'G Cuts at: 830 2942 830 Size: 2112 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5590 PvuII CAG'CTG Cuts at: 2101 2101 Size: 5590 SalI G'TCGA_C Cuts at: 1936 2932 1936 Size: 996 4594 SbfI CC_TGCA'GG Cuts at: 2942 2942 Size: 5590 ScaI AGT'ACT Cuts at: 3838 5550 3838 Size: 1712 3878 SfcI C'TryA_G Cuts at: 826 1045 2938 4073 4754 4945 826 Size: 219 1893 1135 681 191 1471 Fragments arranged by size: 1893 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5590 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5590 SmaI CCC'GGG Cuts at: 1872 2917 1872 Size: 1045 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2291 2716 2876 3694 4595 4857 Size: 197 926 348 425 160 818 901 262 Cuts at: 4857 820 Size: 1553 Fragments arranged by size: 1553 926 901 818 425 348 262 197 160 SnaBI TAC'GTA Cuts at: 493 3381 493 Size: 2888 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5590 SphI G_CATG'C Cuts at: 1132 2948 1132 Size: 1816 3774 SspI AAT'ATT Cuts at: 5 52 3514 5 Size: 47 3462 2081 Fragments arranged by size: 3462 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5590 StyI C'CwwG_G Cuts at: 513 1065 2004 2419 2610 3029 513 Size: 552 939 415 191 419 3074 Fragments arranged by size: 3074 939 552 419 415 191 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3617 3634 3787 3972 4417 325 Size: 1255 2037 17 153 185 445 1498 Fragments arranged by size: 2037 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2136 2558 3836 Size: 274 80 33 51 1443 159 422 1278 Cuts at: 3836 5548 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1278 422 274 159 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5550 5582 5550 Size: 32 5558 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5590 XbaI T'CTAG_A Cuts at: 2926 2926 Size: 5590 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2227 2227 Size: 5590 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5590 XmaI C'CCGG_G Cuts at: 1870 2915 1870 Size: 1045 4545 XmnI GAAnn'nnTTC Cuts at: 3719 3719 Size: 5590 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2617 3398 276 Size: 53 83 186 936 1083 781 2468 Fragments arranged by size: 2468 1083 936 781 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI