Restriction map for
pFN21AB5232
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII AccI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI AspEI AspI AviII
AvrII BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BpuAI BsaAI BsaI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Cfr10I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI HincII HindII HindIII
HpaI KspI MfeI MflI MluI
MslI MunI NaeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 6 CGCG CG^CG
AccIII 1266 TCCGGA T^CCGGA
AclI 1135 AACGTT AA^CGTT
AlwI 1200 GATCC GGATC(4/5)
AocI 1498 CCTCAGG CC^T[ACGT]AGG
ApaI 136 GGGCCC GGGCC^C
Asp700I 469 GAACGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1053 GAGCAC G[AT]GC[AT]^C
AvaI 1064 CCCGGG C^[CT]CG[GA]G
BfaI 643 CTAG C^TAG
BglI 31 GCCCTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1434 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1434 GCTGAGC GC^T[ACGT]AGC
BsaBI 802 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1266 TCCGGA [AT]^CCGG[AT]
BseAI 1266 TCCGGA T^CCGGA
BsiHKAI 1053 GAGCAC G[AT]GC[AT]^C
BsmFI 1229 GGGAC GGGAC(10/14)
BsmI 473 GCATTC GAATGC(1/-1)
BsoBI 1064 CCCGGG C^[CT]CG[GA]G
BspEI 1266 TCCGGA T^CCGGA
BspMI 1181 ACCTGC ACCTGC(4/8)
BstUI 6 CGCG CG^CG
BstXI 315 CCAAAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1498 CCTCAGG CC^T[ACGT]AGG
CelII 1434 GCTGAGC GC^T[ACGT]AGC
DraIII 1236 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 700 CCATGG C^C[GA][CT]GG
Eco81I 1498 CCTCAGG CC^T[ACGT]AGG
EcoNI 1182 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MaeI 643 CTAG C^TAG
MaeII 1136 ACGT A^CGT
MamI 802 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1266 TCCGGA T^CCGGA
MvnI 6 CGCG CG^CG
MwoI 31 GCCCTCTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 700 CCATGG C^CATGG
NdeI 778 CATATG CA^TATG
PpuMI 1229 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 1135 AACGTT AA^CGTT
SapI 456 GAAGAGC GCTCTTC(1/4)
SfaNI 835 GATGC GCATC(5/9)
SmaI 1064 CCCGGG CCC^GGG
SphI 1339 GCATGC GCATG^C
TaqI 182 TCGA T^CGA
TfiI 807 GAATC G^A[AT]TC
TthHB8I 182 TCGA T^CGA
XmaCI 1064 CCCGGG C^CCGGG
XmaI 1064 CCCGGG C^CCGGG
XmnI 469 GAACGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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Acc65I 1175 GGTACC G^GTACC
1192 GGTACC
AcsI 712 AAATTC [GA]^AATT[CT]
1402 AAATTT
AcyI 113 GGCGCC G[GA]^CG[CT]C
143 GGCGCC
ApoI 712 AAATTC [GA]^AATT[CT]
1402 AAATTT
Asp718I 1175 GGTACC G^GTACC
1192 GGTACC
BbeI 113 GGCGCC GGCGC^C
143 GGCGCC
BpmI 196 CTGGAG CTGGAG(16/14)
1279 CTGGAG
BsaHI 113 GGCGCC G[GA]^CG[CT]C
143 GGCGCC
BseRI 481 GAGGAG GAGGAG(10/8)
514 GAGGAG
BsgI 1222 CTGCAC GTGCAG(16/14)
1105 GTGCAG
BsmAI 824 GAGAC GTCTC(1/5)
940 GAGAC
DpnII 549 GATC ^GATC
1200 GATC
FauI 129 CCCGC CCCGC(4/6)
1335 GCGGG
Hin1I 113 GGCGCC G[GA]^CG[CT]C
143 GGCGCC
KasI 113 GGCGCC G^GCGCC
143 GGCGCC
KpnI 1175 GGTACC GGTAC^C
1192 GGTACC
MboI 549 GATC ^GATC
1200 GATC
MspA1I 205 CAGCTG C[AC]G^C[GT]G
628 CAGCTG
NarI 113 GGCGCC GG^CGCC
143 GGCGCC
NdeII 549 GATC ^GATC
1200 GATC
NspI 676 ACATGC [GA]CATG^[CT]
1339 GCATGC
PflMI 1235 CCACAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1272 CCATTTTCTGG
PleI 57 GACTC GAGTC(4/5)
1069 GAGTC
PvuII 205 CAGCTG CAG^CTG
628 CAGCTG
Sau3AI 549 GATC ^GATC
1200 GATC
SfoI 113 GGCGCC GGC^GCC
143 GGCGCC
Van91I 1235 CCACAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1272 CCATTTTCTGG
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# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BalI BanI BanII BbvI BcnI
BmyI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DraII EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeIII MboII MluNI MnlI
MscI MseI MspI MvaI NciI
NlaIII NlaIV PstI RsaI Sau96I
ScrFI SfcI StyI Tru9I TseI
Tsp45I Tsp509I TspRI