(Circular) (Six-base) MAPSORT of: pFN21AB5232.seq Check: 8311 from: 1 to: 6211 pFN21AB5232 6211 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3195 3195 Size: 6211 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4021 278 Size: 53 83 186 936 2485 2468 Fragments arranged by size: 2485 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3554 1937 Size: 1617 4594 Acc65I G'GTAC_C Cuts at: 3180 3197 3532 3180 Size: 17 335 5859 Fragments arranged by size: 5859 335 17 AclI AA'CG_TT Cuts at: 1563 3141 4338 4711 1563 Size: 1578 1197 373 3063 Fragments arranged by size: 3063 1578 1197 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2526 2660 2731 3400 3467 4274 5658 5926 Size: 894 134 71 669 67 807 1384 268 Cuts at: 5926 1632 Size: 1917 Fragments arranged by size: 1917 1384 894 807 669 268 134 71 67 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6014 AflIII A'CryG_T Cuts at: 1203 5110 6087 1203 Size: 3907 977 1327 Fragments arranged by size: 3907 1327 977 AgeI A'CCGG_T Cuts at: 5042 5042 Size: 6211 AhdI GACnn_n'nnGTC Cuts at: 4940 4940 Size: 6211 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6211 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6211 AlwNI CAG_nnn'CTG Cuts at: 2635 2638 2754 3954 5526 2635 Size: 3 116 1200 1572 3320 Fragments arranged by size: 3320 1572 1200 116 3 ApaI G_GGCC'C Cuts at: 2145 2145 Size: 6211 ApaLI G'TGCA_C Cuts at: 4268 5424 4268 Size: 1156 5055 ApoI r'AATT_y Cuts at: 1493 2717 3407 3520 3746 3898 6007 1493 Size: 1224 690 113 226 152 2109 1697 Fragments arranged by size: 2109 1697 1224 690 226 152 113 AseI AT'TA_AT Cuts at: 160 1032 4765 160 Size: 872 3733 1606 Fragments arranged by size: 3733 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6211 AvaI C'yCGr_G Cuts at: 1870 1943 3069 3536 1870 Size: 73 1126 467 4545 Fragments arranged by size: 4545 1126 467 73 BamHI G'GATC_C Cuts at: 3541 3908 3541 Size: 367 5844 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2118 2148 3180 3197 3532 Size: 325 217 627 331 30 1032 17 335 Cuts at: 3532 4987 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1032 627 335 331 325 217 30 17 BanII G_rGCy'C Cuts at: 1391 2024 2145 2974 3530 1391 Size: 633 121 829 556 4072 Fragments arranged by size: 4072 829 633 556 121 BbeI G_GCGC'C Cuts at: 2122 2152 2122 Size: 30 6181 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5240 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4402 4436 1903 Size: 34 50 34 2381 34 3678 Fragments arranged by size: 3678 2381 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4103 5313 4103 Size: 1210 5001 BclI T'GATC_A Cuts at: 1548 1548 Size: 6211 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2042 4822 136 Size: 107 122 71 631 605 370 2780 1525 Fragments arranged by size: 2780 1525 631 605 370 122 107 71 BglII A'GATC_T Cuts at: 6206 6206 Size: 6211 BlpI GC'TnA_GC Cuts at: 3440 3628 3440 Size: 188 6023 Bme1580I G_kGCm'C Cuts at: 1790 2078 2145 4272 5428 1790 Size: 288 67 2127 1156 2573 Fragments arranged by size: 2573 2127 1156 288 67 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6211 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2081 3470 3942 Size: 416 53 83 399 24 664 1389 472 Cuts at: 3942 4900 442 Size: 958 1753 Fragments arranged by size: 1753 1389 958 664 472 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6211 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2222 3305 4871 1239 Size: 983 1083 1566 2579 Fragments arranged by size: 2579 1566 1083 983 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4336 5201 5499 4336 Size: 865 298 5048 Fragments arranged by size: 5048 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4874 882 Size: 617 3375 2219 Fragments arranged by size: 3375 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4002 493 Size: 711 2798 2702 Fragments arranged by size: 2798 2702 711 BsaBI GATnn'nnATC Cuts at: 2811 6205 2811 Size: 3394 2817 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2119 2149 4018 4400 Size: 53 83 186 936 586 30 1869 382 Cuts at: 4400 5035 275 Size: 635 1451 Fragments arranged by size: 1869 1451 936 635 586 382 186 83 53 30 BsaWI w'CCGG_w Cuts at: 3271 4644 5042 5316 5463 5874 3271 Size: 1373 398 274 147 411 3608 Fragments arranged by size: 3608 1373 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5986 Fragments arranged by size: 5986 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2501 2534 1186 Size: 1315 33 4863 Fragments arranged by size: 4863 1315 33 BseYI C'CCAG_C Cuts at: 3176 5414 5910 3176 Size: 2238 496 3477 Fragments arranged by size: 3477 2238 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3131 3212 1163 Size: 573 1395 81 4162 Fragments arranged by size: 4162 1395 573 81 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3661 4422 5450 1540 Size: 275 186 1660 761 1028 2301 Fragments arranged by size: 2301 1660 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3062 4272 4357 5428 3062 Size: 1210 85 1071 3845 Fragments arranged by size: 3845 1210 1071 85 BsmI GAATG_Cn' Cuts at: 2478 3722 3815 2478 Size: 1244 93 4874 Fragments arranged by size: 4874 1244 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2078 2145 2974 3062 3530 4272 Size: 399 234 54 67 829 88 468 742 Cuts at: 4272 4357 5428 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 829 742 468 399 234 88 85 67 54 BspEI T'CCGG_A Cuts at: 3271 5874 3271 Size: 2603 3608 BspHI T'CATG_A Cuts at: 4100 5830 4100 Size: 1730 4481 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3195 3566 844 Size: 2351 371 3489 Fragments arranged by size: 3489 2351 371 BsrBI CCG'CTC Cuts at: 1394 1823 4098 1394 Size: 429 2275 3507 Fragments arranged by size: 3507 2275 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4706 4880 65 Size: 1183 3458 174 1396 Fragments arranged by size: 3458 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4855 5042 1700 Size: 110 3045 187 2869 Fragments arranged by size: 3045 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6211 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6211 BssSI C'ACGA_G Cuts at: 4271 5283 4271 Size: 1012 5199 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6211 BstBI TT'CG_AA Cuts at: 5015 5015 Size: 6211 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6211 BstXI CCAn_nnnn'nTGG Cuts at: 2327 2327 Size: 6211 BstYI r'GATC_y Cuts at: 1972 3541 3908 4293 4310 5762 5848 5860 6206 Size: 1569 367 385 17 1452 86 12 346 Cuts at: 6206 1972 Size: 1977 Fragments arranged by size: 1977 1569 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3504 3504 Size: 6211 BtgI C'CryG_G Cuts at: 513 1065 2705 513 Size: 552 1640 4019 Fragments arranged by size: 4019 1640 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2030 2980 487 Size: 683 56 804 950 3718 Fragments arranged by size: 3718 950 804 683 56 BtsI GCAGTG_nn' Cuts at: 2052 3727 4521 4541 2052 Size: 1675 794 20 3722 Fragments arranged by size: 3722 1675 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6211 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6176 DraI TTT'AAA Cuts at: 3515 3515 Size: 6211 DraIII CAC_nnn'GTG Cuts at: 3246 3246 Size: 6211 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5218 809 Size: 507 3902 1802 Fragments arranged by size: 3902 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2395 3040 3363 Size: 54 1425 50 275 18 565 645 323 Cuts at: 3363 3658 4547 6176 8 Size: 295 889 1629 43 Fragments arranged by size: 1629 1425 889 645 565 323 295 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3658 1537 Size: 275 1846 4090 Fragments arranged by size: 4090 1846 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2456 2750 2933 4141 1353 Size: 1103 294 183 1208 3423 Fragments arranged by size: 3423 1208 1103 294 183 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4798 5172 5318 387 Size: 1064 138 3209 374 146 1280 Fragments arranged by size: 3209 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2222 2526 2660 2731 3305 3400 3467 Size: 393 590 304 134 71 574 95 67 Cuts at: 3467 4274 4871 5658 5926 1239 Size: 807 597 787 268 1524 Fragments arranged by size: 1524 807 787 597 590 574 393 304 268 134 95 71 67 EcoNI CCTnn'n_nnAGG Cuts at: 3191 3191 Size: 6211 EcoO109I rG'GnC_Cy Cuts at: 2141 2142 3235 3420 2141 Size: 1 1093 185 4932 Fragments arranged by size: 4932 1093 185 1 EcoRI G'AATT_C Cuts at: 3520 3898 3520 Size: 378 5833 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2321 2482 2321 Size: 161 6050 FspI TGC'GCA Cuts at: 4717 4717 Size: 6211 HaeII r_GCGC'y Cuts at: 1678 2122 2152 3227 5358 1678 Size: 444 30 1075 2131 2531 Fragments arranged by size: 2531 2131 1075 444 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2008 2040 2346 2378 4852 Size: 32 135 32 436 32 306 32 2474 Cuts at: 4852 4884 4926 4958 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2474 436 306 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3555 3801 669 Size: 988 281 1617 246 3079 Fragments arranged by size: 3079 1617 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6211 HpaI GTT'AAC Cuts at: 3801 3801 Size: 6211 KasI G'GCGC_C Cuts at: 2118 2148 2118 Size: 30 6181 KpnI G_GTAC'C Cuts at: 3184 3201 3536 3184 Size: 17 335 5859 Fragments arranged by size: 5859 335 17 MfeI C'AATT_G Cuts at: 3810 3810 Size: 6211 MluI A'CGCG_T Cuts at: 6087 6087 Size: 6211 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5325 5509 1305 Size: 4020 184 2007 Fragments arranged by size: 4020 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2397 3042 3365 10 Size: 54 1425 343 565 645 323 2856 Fragments arranged by size: 2856 1425 645 565 343 323 54 MslI CAynn'nnrTG Cuts at: 518 1166 4169 4528 4687 518 Size: 648 3003 359 159 2042 Fragments arranged by size: 3003 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2212 2635 3627 4304 5452 5697 1606 Size: 606 423 992 677 1148 245 2120 Fragments arranged by size: 2120 1148 992 677 606 423 245 NarI GG'CG_CC Cuts at: 2119 2149 2119 Size: 30 6181 NcoI C'CATG_G Cuts at: 513 1065 2705 513 Size: 552 1640 4019 Fragments arranged by size: 4019 1640 552 NdeI CA'TA_TG Cuts at: 387 2784 387 Size: 2397 3814 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6211 NotI GC'GGCC_GC Cuts at: 3658 3658 Size: 6211 NspI r_CATG'y Cuts at: 1132 1229 2685 3348 3569 5114 1132 Size: 97 1456 663 221 1545 2229 Fragments arranged by size: 2229 1545 1456 663 221 97 PasI CC'CwG_GG Cuts at: 1348 3147 1348 Size: 1799 4412 PciI A'CATG_T Cuts at: 5110 5110 Size: 6211 PflMI CCAn_nnn'nTGG Cuts at: 1108 3246 3283 1108 Size: 2138 37 4036 Fragments arranged by size: 4036 2138 37 PmeI GTTT'AAAC Cuts at: 3515 3515 Size: 6211 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4280 4312 5793 5825 4280 Size: 32 1481 32 4666 Fragments arranged by size: 4666 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3235 3235 Size: 6211 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6211 PsiI TTA'TAA Cuts at: 3781 3781 Size: 6211 PspOMI G'GGCC_C Cuts at: 2141 2141 Size: 6211 PstI C_TGCA'G Cuts at: 830 2269 2821 2915 3563 830 Size: 1439 552 94 648 3478 Fragments arranged by size: 3478 1439 648 552 94 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6211 PvuII CAG'CTG Cuts at: 2212 2635 2212 Size: 423 5788 SalI G'TCGA_C Cuts at: 1936 3553 1936 Size: 1617 4594 SanDI GG'GwC_CC Cuts at: 3235 3235 Size: 6211 SapI GCTCTTCn'nnn_ Cuts at: 2456 2456 Size: 6211 SbfI CC_TGCA'GG Cuts at: 3563 3563 Size: 6211 ScaI AGT'ACT Cuts at: 4459 6171 4459 Size: 1712 4499 SfcI C'TryA_G Cuts at: 826 1045 2265 2817 2911 3559 4694 5375 5566 Size: 219 1220 552 94 648 1135 681 191 Cuts at: 5566 826 Size: 1471 Fragments arranged by size: 1471 1220 1135 681 648 552 219 191 94 SfoI GGC'GCC Cuts at: 2120 2150 2120 Size: 30 6181 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6211 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6211 SmaI CCC'GGG Cuts at: 1872 3071 3538 1872 Size: 1199 467 4545 Fragments arranged by size: 4545 1199 467 SmlI C'TyrA_G Cuts at: 820 1017 1943 4315 5216 5478 820 Size: 197 926 2372 901 262 1553 Fragments arranged by size: 2372 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4002 493 Size: 3509 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6211 SphI G_CATG'C Cuts at: 1132 3348 3569 1132 Size: 2216 221 3774 Fragments arranged by size: 3774 2216 221 SspI AAT'ATT Cuts at: 5 52 4135 5 Size: 47 4083 2081 Fragments arranged by size: 4083 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6211 StyI C'CwwG_G Cuts at: 513 1065 2161 2705 3192 3650 513 Size: 552 1096 544 487 458 3074 Fragments arranged by size: 3074 1096 552 544 487 458 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4238 4255 4408 4593 5038 325 Size: 1255 2658 17 153 185 445 1498 Fragments arranged by size: 2658 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3013 4457 6169 Size: 274 80 33 51 1443 1036 1444 1712 Cuts at: 6169 96 Size: 138 Fragments arranged by size: 1712 1444 1443 1036 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2860 2892 3253 3285 6171 6203 2860 Size: 32 361 32 2886 32 2868 Fragments arranged by size: 2886 2868 361 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6211 XbaI T'CTAG_A Cuts at: 3547 3547 Size: 6211 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6211 XmaI C'CCGG_G Cuts at: 1870 3069 3536 1870 Size: 1199 467 4545 Fragments arranged by size: 4545 1199 467 XmnI GAAnn'nnTTC Cuts at: 2478 4340 2478 Size: 1862 4349 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4019 276 Size: 53 83 186 936 2485 2468 Fragments arranged by size: 2485 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SexAI SfiI SrfI SwaI XcmI