Restriction map for
pFN21AB0487
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI AscI
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BamHI BanI BbeI
BbvCI BcgI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaHI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BsrI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco52I Eco81I EcoO109I EcoO65I EcoT14I
EcoT22I FauI FbaI FseI FspI
HapII HgaI Hin1I HincII HindII
HpaI HpaII KasI KpnI KspI
MflI MluI MroI MspI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI StuI StyI SwaI TspRI
Tth111I Van91I XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 472 GTAGAC GT^[AC][GT]AC
AciI 256 CCGC CCGC(-3/-1)
AfaI 1520 GTAC GT^AC
AlwI 1285 GATCC GGATC(4/5)
AlwNI 263 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 460 AGCGCT AGC^GCT
AseI 375 ATTAAT AT^TAAT
AsnI 375 ATTAAT AT^TAAT
AspHI 884 GAGCAC G[AT]GC[AT]^C
BanII 980 GAGCCC G[GA]GC[CT]^C
BbrPI 395 CACGTG CAC^GTG
BpmI 101 CTCCAG CTGGAG(16/14)
BsaAI 395 CACGTG [CT]AC^GT[GA]
BsaBI 783 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1015 GGTCTC GGTCTC(1/5)
BsiHKAI 884 GAGCAC G[AT]GC[AT]^C
BsmFI 1052 GTCCC GGGAC(10/14)
BspMI 904 GCAGGT ACCTGC(4/8)
BssSI 1036 CTCGTG CACGAG(-5/-1)
CfoI 461 GCGC GCG^C
Cfr13I 1041 GGGCC G^G[ACGT]CC
DpnII 1285 GATC ^GATC
DraI 915 TTTAAA TTT^AAA
EarI 1408 GAAGAG CTCTTC(1/4)
Eco47III 460 AGCGCT AGC^GCT
EcoNI 574 CCTGAATTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 353 GAATTC G^AATTC
EcoRV 717 GATATC GAT^ATC
HaeII 460 AGCGCT [GA]GCGC^[CT]
HhaI 461 GCGC GCG^C
HinP1I 461 GCGC G^CGC
HindIII 661 AAGCTT A^AGCTT
Ksp632I 1408 GAAGAG CTCTTC(1/4)
MamI 783 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 1285 GATC ^GATC
MfeI 1532 CAATTG C^AATTG
MunI 1532 CAATTG C^AATTG
NdeII 1285 GATC ^GATC
NspI 930 GCATGC [GA]CATG^[CT]
PleI 1297 GACTC GAGTC(4/5)
PmaCI 395 CACGTG CAC^GTG
PmlI 395 CACGTG CAC^GTG
PshBI 375 ATTAAT AT^TAAT
PstI 710 CTGCAG CTGCA^G
RsaI 1520 GTAC GT^AC
Sau3AI 1285 GATC ^GATC
Sau96I 1041 GGGCC G^G[ACGT]CC
SfcI 710 CTGCAG C^T[GA][CT]AG
SphI 930 GCATGC GCATG^C
SspI 757 AATATT AAT^ATT
Tsp45I 1295 GTGAC ^GT[GC]AC
XbaI 991 TCTAGA T^CTAGA
XcmI 1091 CCAAAACCGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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Asp700I 353 GAATTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1067 GAAAGATTTC
BalI 839 TGGCCA TGG^CCA
923 TGGCCA
BbsI 1195 GAAGAC GAAGAC(2/6)
1275 GAAGAC
BmyI 884 GAGCAC G[AGT]GC[ACT]^C
980 GAGCCC
BpuAI 1195 GAAGAC GAAGAC(2/6)
1275 GAAGAC
BsiYI 574 CCTGAATTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1006 CCTAAGTATGG
BslI 574 CCTGAATTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1006 CCTAAGTATGG
BsmAI 438 GTCTC GTCTC(1/5)
1016 GTCTC
BsmI 1079 GAATGC GAATGC(1/-1)
175 GCATTC
Bsp1286I 884 GAGCAC G[AGT]GC[ACT]^C
980 GAGCCC
BsrDI 843 CATTGC GCAATG(2/0)
1144 GCAATG
BstF5I 369 GGATG GGATG(2/0)
1353 GGATG
EaeI 839 TGGCCA [CT]^GGCC[GA]
923 TGGCCA
FokI 369 GGATG GGATG(9/13)
1353 GGATG
MaeII 19 ACGT A^CGT
396 ACGT
MluNI 839 TGGCCA TGG^CCA
923 TGGCCA
MscI 839 TGGCCA TGG^CCA
923 TGGCCA
MslI 774 CACCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1261 CACAAAAGTG
PvuII 211 CAGCTG CAG^CTG
707 CAGCTG
XmnI 353 GAATTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1067 GAAAGATTTC
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AcsI AluI ApoI BbvI BciVI
BfaI BsaJI BssKI BstNI Cac8I
DdeI EcoRII Fnu4HI HaeIII HinfI
HphI ItaI MaeI MaeIII MboII
MnlI MseI MspA1I MvaI MwoI
NlaIII ScrFI SfaNI TaqI TfiI
Tru9I TseI Tsp509I TthHB8I