################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BsiWI BsmAI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI Eam1105I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI KspI MamI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tsp45I Tth111I TthHB8I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AcsI 179 AAATTT [GA]^AATT[CT] AlwI 390 GATCC GGATC(4/5) Aor51HI 704 AGCGCT AGC^GCT ApoI 179 AAATTT [GA]^AATT[CT] AspHI 199 GAGCTC G[AT]GC[AT]^C AvaI 46 CCCGGG C^[CT]CG[GA]G BalI 449 TGGCCA TGG^CCA BanI 238 GGCACC G^G[CT][GA]CC BsaWI 267 ACCGGA [AT]^CCGG[AT] BseRI 433 GAGGAG GAGGAG(10/8) BsgI 96 GTGCAG GTGCAG(16/14) BsiHKAI 199 GAGCTC G[AT]GC[AT]^C BsmFI 491 GGGAC GGGAC(10/14) BsmI 73 GAATGC GAATGC(1/-1) BsoBI 46 CCCGGG C^[CT]CG[GA]G BsrI 594 ACTGG ACTGG(1/-1) BstYI 389 AGATCC [GA]^GATC[CT] DpnII 390 GATC ^GATC DsaI 356 CCATGG C^C[GA][CT]GG EagI 119 CGGCCG C^GGCCG EclXI 119 CGGCCG C^GGCCG Eco47III 704 AGCGCT AGC^GCT Eco52I 119 CGGCCG C^GGCCG EcoT14I 356 CCATGG C^C[AT][AT]GG FauI 66 GCGGG CCCGC(4/6) HaeII 704 AGCGCT [GA]GCGC^[CT] HinfI 442 GACTC G^A[ACGT]TC MaeII 94 ACGT A^CGT MaeIII 746 GTTAC ^GT[ACGT]AC MboI 390 GATC ^GATC MflI 389 AGATCC [GA]^GATC[CT] MluNI 449 TGGCCA TGG^CCA MscI 449 TGGCCA TGG^CCA MseI 577 TTAA T^TAA MslI 514 CACCAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 208 CAGCTG C[AC]G^C[GT]G NcoI 356 CCATGG C^CATGG NdeII 390 GATC ^GATC NheI 285 GCTAGC G^CTAGC PleI 442 GACTC GAGTC(4/5) PvuII 208 CAGCTG CAG^CTG SacI 199 GAGCTC GAGCT^C Sau3AI 390 GATC ^GATC SfaNI 427 GATGC GCATC(5/9) SmaI 46 CCCGGG CCC^GGG SmlI 230 CTCAAG C^T[CT][GA]AG StyI 356 CCATGG C^C[AT][AT]GG Tru9I 577 TTAA T^TAA TspRI 525 CAGTG CA[GC]TG[ACGT][ACGT]^ XhoII 389 AGATCC [GA]^GATC[CT] XmaCI 46 CCCGGG C^CCGGG XmaI 46 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccII 5 CGCG CG^CG 90 CGCG BanII 49 GGGCTC G[GA]GC[CT]^C 199 GAGCTC BfaI 109 CTAG C^TAG 286 CTAG BmyI 49 GGGCTC G[AGT]GC[ACT]^C 199 GAGCTC BsiEI 59 CGACCG CG[GA][CT]^CG 119 CGGCCG Bsp1286I 49 GGGCTC G[AGT]GC[ACT]^C 199 GAGCTC BspMI 190 ACCTGC ACCTGC(4/8) 464 ACCTGC BstUI 5 CGCG CG^CG 90 CGCG BstXI 513 CCACCAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 658 CCAGAGTGGTGG Cfr13I 43 GGCCC G^G[ACGT]CC 68 GGGCC DdeI 250 CTCAG C^T[ACGT]AG 459 CTCAG EaeI 119 CGGCCG [CT]^GGCC[GA] 449 TGGCCA EarI 370 GAAGAG CTCTTC(1/4) 644 GAAGAG Ksp632I 370 GAAGAG CTCTTC(1/4) 644 GAAGAG MaeI 109 CTAG C^TAG 286 CTAG MvnI 5 CGCG CG^CG 90 CGCG PstI 205 CTGCAG CTGCA^G 616 CTGCAG Sau96I 43 GGCCC G^G[ACGT]CC 68 GGGCC SfcI 205 CTGCAG C^T[GA][CT]AG 616 CTGCAG Tsp509I 180 AATT ^AATT 454 AATT XcmI 510 CCACCACCAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 678 CCAGAAACGGTATGG ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AciI AluI BbvI BcnI BsaJI BsiYI BslI BspHI BssKI BstF5I BstNI Cac8I CfoI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MboII MnlI MspI MvaI MwoI NciI NlaIII NlaIV RcaI ScrFI TseI