################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BseRI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DpnII DraI DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HgaI HincII HindII HindIII HpaI HphI KasI KpnI KspI MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MspA1I MunI MvnI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI Sau3AI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 27 enzymes) # ################################### AatII 259 GACGTC GACGT^C AcyI 259 GACGTC G[GA]^CG[CT]C ApaI 600 GGGCCC GGGCC^C AspHI 568 GAGCTC G[AT]GC[AT]^C AspI 304 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbsI 283 GAAGAC GAAGAC(2/6) BpuAI 283 GAAGAC GAAGAC(2/6) BsaHI 259 GACGTC G[GA]^CG[CT]C BsaI 309 GGTCTC GGTCTC(1/5) BsgI 519 CTGCAC GTGCAG(16/14) BsiHKAI 568 GAGCTC G[AT]GC[AT]^C BsmBI 194 CGTCTC CGTCTC(1/5) BsmFI 682 GGGAC GGGAC(10/14) BsrI 68 ACTGG ACTGG(1/-1) BstAPI 78 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DrdI 253 GACTATGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 293 TGGCCG [CT]^GGCC[GA] EarI 102 GAAGAG CTCTTC(1/4) EcoNI 492 CCTGACCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 379 GGCGCT [GA]GCGC^[CT] Hin1I 259 GACGTC G[GA]^CG[CT]C Ksp632I 102 GAAGAG CTCTTC(1/4) NspI 216 ACATGC [GA]CATG^[CT] PflFI 304 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC SacI 568 GAGCTC GAGCT^C Tsp509I 693 AATT ^AATT Tth111I 304 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AciI 296 CCGC CCGC(-3/-1) 377 GCGG AfaI 474 GTAC GT^AC 582 GTAC BanII 568 GAGCTC G[GA]GC[CT]^C 600 GGGCCC BbvCI 180 CCTCAGC CCTCAGC(-5/-2) 543 CCTCAGC BfaI 52 CTAG C^TAG 706 CTAG BmyI 568 GAGCTC G[AGT]GC[ACT]^C 600 GGGCCC BsmAI 195 GTCTC GTCTC(1/5) 310 GTCTC Bsp1286I 568 GAGCTC G[AGT]GC[ACT]^C 600 GGGCCC BstF5I 525 CATCC GGATG(2/0) 363 GGATG CfoI 342 GCGC GCG^C 380 GCGC FokI 525 CATCC GGATG(9/13) 363 GGATG HhaI 342 GCGC GCG^C 380 GCGC HinP1I 342 GCGC G^CGC 380 GCGC HinfI 575 GATTC G^A[ACGT]TC 736 GATTC MaeI 52 CTAG C^TAG 706 CTAG MslI 217 CATGCGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 742 CACAAATGTG PflMI 22 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 300 CCAGGACCTGG PstI 484 CTGCAG CTGCA^G 649 CTGCAG RsaI 474 GTAC GT^AC 582 GTAC SexAI 287 ACCTGGT A^CC[AT]GGT 305 ACCTGGT TaqI 10 TCGA T^CGA 590 TCGA TfiI 575 GATTC G^A[AT]TC 736 GATTC TthHB8I 10 TCGA T^CGA 590 TCGA Van91I 22 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 300 CCAGGACCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AluI AlwNI AvaII BbvI BcnI BpmI BsaJI BsiYI BslI BssKI BstNI Cac8I Cfr13I DdeI DraII EcoO109I EcoRII Fnu4HI HaeIII HapII HpaII ItaI MaeII MaeIII MboII MnlI MspI MvaI MwoI NciI NlaIII NlaIV PpuMI Sau96I ScrFI SfaNI SfcI TseI Tsp45I TspRI