(Circular) (Six-base) MAPSORT of: pFN21AB9901.seq Check: 1501 from: 1 to: 5473 pFN21AB9901 5473 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2268 3283 278 Size: 53 83 186 936 732 1015 2468 Fragments arranged by size: 2468 1015 936 732 186 83 53 AccI GT'mk_AC Cuts at: 1937 2816 1937 Size: 879 4594 Acc65I G'GTAC_C Cuts at: 2794 2794 Size: 5473 AclI AA'CG_TT Cuts at: 1563 3600 3973 1563 Size: 2037 373 3063 Fragments arranged by size: 3063 2037 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2114 2444 3536 4920 5188 1632 Size: 482 330 1092 1384 268 1917 Fragments arranged by size: 1917 1384 1092 482 330 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5276 AflIII A'CryG_T Cuts at: 1203 4372 5349 1203 Size: 3169 977 1327 Fragments arranged by size: 3169 1327 977 AgeI A'CCGG_T Cuts at: 4304 4304 Size: 5473 AhdI GACnn_n'nnGTC Cuts at: 4202 4202 Size: 5473 AleI CACnn'nnGTG Cuts at: 1166 2751 1166 Size: 1585 3888 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5473 AlwNI CAG_nnn'CTG Cuts at: 2033 2089 2311 2413 2488 2497 3216 4788 2033 Size: 56 222 102 75 9 719 1572 2718 Fragments arranged by size: 2718 1572 719 222 102 75 56 9 ApaI G_GGCC'C Cuts at: 2609 2609 Size: 5473 ApaLI G'TGCA_C Cuts at: 3530 4686 3530 Size: 1156 4317 ApoI r'AATT_y Cuts at: 1493 2782 3008 3160 5269 1493 Size: 1289 226 152 2109 1697 Fragments arranged by size: 2109 1697 1289 226 152 AseI AT'TA_AT Cuts at: 160 1032 4027 160 Size: 872 2995 1606 Fragments arranged by size: 2995 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5473 AvaI C'yCGr_G Cuts at: 1870 1943 2798 1870 Size: 73 855 4545 Fragments arranged by size: 4545 855 73 BamHI G'GATC_C Cuts at: 2803 3170 2803 Size: 367 5106 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2794 4249 618 Size: 325 217 627 1007 1455 1842 Fragments arranged by size: 1842 1455 1007 627 325 217 BanII G_rGCy'C Cuts at: 1391 2577 2609 2792 1391 Size: 1186 32 183 4072 Fragments arranged by size: 4072 1186 183 32 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2295 928 Size: 971 396 4106 Fragments arranged by size: 4106 971 396 BbvCI CC'TCA_GC Cuts at: 2186 2549 2186 Size: 363 5110 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3664 3698 1903 Size: 34 1727 34 3678 Fragments arranged by size: 3678 1727 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3365 4575 3365 Size: 1210 4263 BclI T'GATC_A Cuts at: 1548 1548 Size: 5473 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4084 136 Size: 107 122 71 631 605 2412 1525 Fragments arranged by size: 2412 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5468 5468 Size: 5473 BlpI GC'TnA_GC Cuts at: 2890 2890 Size: 5473 Bme1580I G_kGCm'C Cuts at: 1790 2609 3534 4690 1790 Size: 819 925 1156 2573 Fragments arranged by size: 2573 1156 925 819 BmgBI CAC'GTC Cuts at: 1318 2199 1318 Size: 881 4592 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3204 4162 442 Size: 416 53 83 399 24 1787 958 1753 Fragments arranged by size: 1787 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5473 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2119 2269 2493 4133 1239 Size: 880 150 224 1640 2579 Fragments arranged by size: 2579 1640 880 224 150 Bpu10I CC'TnA_GC Cuts at: 2186 2549 2571 2186 Size: 363 22 5088 Fragments arranged by size: 5088 363 22 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3598 4463 4761 3598 Size: 865 298 4310 Fragments arranged by size: 4310 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2320 4136 882 Size: 617 821 1816 2219 Fragments arranged by size: 2219 1816 821 617 BsaAI yAC'GTr Cuts at: 493 1204 3264 493 Size: 711 2060 2702 Fragments arranged by size: 2702 2060 711 BsaBI GATnn'nnATC Cuts at: 5467 5467 Size: 5473 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2265 3280 3662 4297 Size: 53 83 186 936 732 1015 382 635 Cuts at: 4297 275 Size: 1451 Fragments arranged by size: 1451 1015 936 732 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3906 4304 4578 4725 5136 3906 Size: 398 274 147 411 4243 Fragments arranged by size: 4243 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2300 2330 2491 2521 2559 Size: 30 165 30 897 30 161 30 38 Cuts at: 2559 2589 1178 Size: 30 4062 Fragments arranged by size: 4062 897 165 161 38 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5473 BseYI C'CCAG_C Cuts at: 2026 2204 4676 5172 2026 Size: 178 2472 496 2327 Fragments arranged by size: 2472 2327 496 178 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2509 1163 Size: 573 773 4127 Fragments arranged by size: 4127 773 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2923 3684 4712 1540 Size: 275 186 922 761 1028 2301 Fragments arranged by size: 2301 1028 922 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2577 3534 3619 4690 2577 Size: 957 85 1071 3360 Fragments arranged by size: 3360 1071 957 85 BsmI GAATG_Cn' Cuts at: 2984 3077 2984 Size: 93 5380 BsmBI CGTCTCn'nnnn_ Cuts at: 2205 2205 Size: 5473 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2577 2609 2792 3534 3619 4690 1391 Size: 399 787 32 183 742 85 1071 2174 Fragments arranged by size: 2174 1071 787 742 399 183 85 32 BspEI T'CCGG_A Cuts at: 5136 5136 Size: 5473 BspHI T'CATG_A Cuts at: 3362 5092 3362 Size: 1730 3743 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2828 844 Size: 1984 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3360 1394 Size: 429 1537 3507 Fragments arranged by size: 3507 1537 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3968 4142 65 Size: 1183 2720 174 1396 Fragments arranged by size: 2720 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4117 4304 1700 Size: 110 2307 187 2869 Fragments arranged by size: 2869 2307 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5473 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5473 BssSI C'ACGA_G Cuts at: 3533 4545 3533 Size: 1012 4461 BstAPI GCAn_nnn'nTGC Cuts at: 794 2089 794 Size: 1295 4178 BstBI TT'CG_AA Cuts at: 4277 4277 Size: 5473 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5473 BstYI r'GATC_y Cuts at: 1972 2803 3170 3555 3572 5024 5110 5122 5468 Size: 831 367 385 17 1452 86 12 346 Cuts at: 5468 1972 Size: 1977 Fragments arranged by size: 1977 1452 831 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3982 Fragments arranged by size: 3982 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2154 487 Size: 683 56 928 3806 Fragments arranged by size: 3806 928 683 56 BtsI GCAGTG_nn' Cuts at: 2334 2425 2989 3783 3803 2334 Size: 91 564 794 20 4004 Fragments arranged by size: 4004 794 564 91 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5473 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5438 DraI TTT'AAA Cuts at: 2777 2777 Size: 5473 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2264 4480 809 Size: 507 948 2216 1802 Fragments arranged by size: 2216 1802 948 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2298 2920 3809 Size: 54 1425 50 275 18 468 622 889 Cuts at: 3809 5438 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 622 468 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2920 1537 Size: 275 1108 4090 Fragments arranged by size: 4090 1108 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2102 3403 1353 Size: 749 1301 3423 Fragments arranged by size: 3423 1301 749 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4060 4434 4580 387 Size: 1064 138 2471 374 146 1280 Fragments arranged by size: 2471 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2575 2575 Size: 5473 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 2119 2269 2444 2493 3536 4133 Size: 393 482 5 150 175 49 1043 597 Cuts at: 4133 4920 5188 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1043 787 597 482 393 268 175 150 49 5 EcoNI CCTnn'n_nnAGG Cuts at: 2501 2501 Size: 5473 EcoO109I rG'GnC_Cy Cuts at: 2308 2464 2494 2605 2606 2688 2733 2308 Size: 156 30 111 1 82 45 5048 Fragments arranged by size: 5048 156 111 82 45 30 1 EcoRI G'AATT_C Cuts at: 2782 3160 2782 Size: 378 5095 FspI TGC'GCA Cuts at: 3979 3979 Size: 5473 HaeII r_GCGC'y Cuts at: 1678 2388 4620 1678 Size: 710 2232 2531 Fragments arranged by size: 2531 2232 710 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2300 2332 2407 2439 2491 Size: 32 135 32 728 32 75 32 52 Cuts at: 2491 2523 4114 4146 4188 4220 1373 Size: 32 1591 32 42 32 2626 Fragments arranged by size: 2626 1591 728 135 75 52 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2817 3063 669 Size: 988 281 879 246 3079 Fragments arranged by size: 3079 988 879 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5473 HpaI GTT'AAC Cuts at: 3063 3063 Size: 5473 KpnI G_GTAC'C Cuts at: 2798 2798 Size: 5473 MfeI C'AATT_G Cuts at: 3072 3072 Size: 5473 MluI A'CGCG_T Cuts at: 5349 5349 Size: 5473 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4587 4771 1305 Size: 3282 184 2007 Fragments arranged by size: 3282 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3651 Fragments arranged by size: 3651 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2226 2751 3431 3790 3949 518 Size: 648 1060 525 680 359 159 2042 Fragments arranged by size: 2042 1060 680 648 525 359 159 MspA1I CmG'CkG Cuts at: 1606 2889 3566 4714 4959 1606 Size: 1283 677 1148 245 2120 Fragments arranged by size: 2120 1283 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3982 Fragments arranged by size: 3982 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5473 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5473 NotI GC'GGCC_GC Cuts at: 2920 2920 Size: 5473 NspI r_CATG'y Cuts at: 1132 1229 2225 2831 4376 1132 Size: 97 996 606 1545 2229 Fragments arranged by size: 2229 1545 996 606 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5473 PciI A'CATG_T Cuts at: 4372 4372 Size: 5473 PflMI CCAn_nnn'nTGG Cuts at: 1108 2033 2311 1108 Size: 925 278 4270 Fragments arranged by size: 4270 925 278 PfoI T'CCnGG_A Cuts at: 2364 2364 Size: 5473 PmeI GTTT'AAAC Cuts at: 2777 2777 Size: 5473 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3542 3574 5055 5087 3542 Size: 32 1481 32 3928 Fragments arranged by size: 3928 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2308 2464 2688 2308 Size: 156 224 5093 Fragments arranged by size: 5093 224 156 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5473 PsiI TTA'TAA Cuts at: 3043 3043 Size: 5473 PspOMI G'GGCC_C Cuts at: 2605 2605 Size: 5473 PstI C_TGCA'G Cuts at: 830 2493 2658 2825 830 Size: 1663 165 167 3478 Fragments arranged by size: 3478 1663 167 165 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5473 SacI G_AGCT'C Cuts at: 2577 2577 Size: 5473 SalI G'TCGA_C Cuts at: 1936 2815 1936 Size: 879 4594 SanDI GG'GwC_CC Cuts at: 2688 2688 Size: 5473 SbfI CC_TGCA'GG Cuts at: 2825 2825 Size: 5473 ScaI AGT'ACT Cuts at: 3721 5433 3721 Size: 1712 3761 SexAI A'CCwGG_T Cuts at: 2292 2310 2292 Size: 18 5455 SfcI C'TryA_G Cuts at: 826 1045 2388 2489 2654 2821 3956 4637 4828 Size: 219 1343 101 165 167 1135 681 191 Cuts at: 4828 826 Size: 1471 Fragments arranged by size: 1471 1343 1135 681 219 191 167 165 101 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5473 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5473 SmaI CCC'GGG Cuts at: 1872 2800 1872 Size: 928 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3577 4478 4740 820 Size: 197 926 1634 901 262 1553 Fragments arranged by size: 1634 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3264 493 Size: 2771 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5473 SphI G_CATG'C Cuts at: 1132 2831 1132 Size: 1699 3774 SspI AAT'ATT Cuts at: 5 52 3397 5 Size: 47 3345 2081 Fragments arranged by size: 3345 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5473 StyI C'CwwG_G Cuts at: 513 1065 2004 2912 513 Size: 552 939 908 3074 Fragments arranged by size: 3074 939 908 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2230 2516 3500 3517 3670 3855 4300 Size: 1255 650 286 984 17 153 185 445 Cuts at: 4300 325 Size: 1498 Fragments arranged by size: 1498 1255 984 650 445 286 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2586 3719 5431 Size: 274 80 33 51 1443 609 1133 1712 Cuts at: 5431 96 Size: 138 Fragments arranged by size: 1712 1443 1133 609 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2456 2488 2559 2591 5433 5465 2456 Size: 32 71 32 2842 32 2464 Fragments arranged by size: 2842 2464 71 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2312 1316 Size: 996 4477 XbaI T'CTAG_A Cuts at: 2809 2809 Size: 5473 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5473 XmaI C'CCGG_G Cuts at: 1870 2798 1870 Size: 928 4545 XmnI GAAnn'nnTTC Cuts at: 3602 3602 Size: 5473 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2266 3281 276 Size: 53 83 186 936 732 1015 2468 Fragments arranged by size: 2468 1015 936 732 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII SapI SfiI SfoI SrfI SwaI XcmI