################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## AatII AccI AccII AclI AcyI AflII AgeI AhdI Aor51HI AscI AseI AsnI AspEI AspI AviII AvrII BbeI BbrPI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsiEI BsiWI BsmBI Bsp1407I BspDI BspHI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FseI FspI Hin1I HindIII HpaI KasI KspI MamI MfeI MluI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 717 GGTACC G^GTACC AflIII 745 ACATGT A^C[GA][CT]GT Alw44I 688 GTGCAC G^TGCAC ApaI 1160 GGGCCC GGGCC^C ApaLI 688 GTGCAC G^TGCAC Asp700I 125 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 717 GGTACC G^GTACC BalI 290 TGGCCA TGG^CCA BamHI 854 GGATCC G^GATCC BbsI 1047 GAAGAC GAAGAC(2/6) BbvCI 280 CCTCAGC CCTCAGC(-5/-2) BpuAI 1047 GAAGAC GAAGAC(2/6) BsmI 485 GAATGC GAATGC(1/-1) BspLU11I 745 ACATGT A^CATGT BsrFI 926 GCCGGT [GA]^CCGG[CT] CfoI 934 GCGC GCG^C Cfr10I 926 GCCGGT [GA]^CCGG[CT] DraIII 1231 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 509 CCATGG C^C[GA][CT]GG EaeI 290 TGGCCA [CT]^GGCC[GA] EcoNI 1482 CCTGCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 75 GAATTC G^AATTC FauI 1437 GCGGG CCCGC(4/6) HaeII 933 GGCGCT [GA]GCGC^[CT] HgaI 396 GACGC GACGC(5/10) HhaI 934 GCGC GCG^C HinP1I 934 GCGC G^CGC KpnI 717 GGTACC GGTAC^C MaeII 836 ACGT A^CGT MluNI 290 TGGCCA TGG^CCA MscI 290 TGGCCA TGG^CCA MseI 473 TTAA T^TAA NcoI 509 CCATGG C^CATGG NspI 745 ACATGT [GA]CATG^[CT] SmaI 267 CCCGGG CCC^GGG Tru9I 473 TTAA T^TAA XmaCI 267 CCCGGG C^CCGGG XmaI 267 CCCGGG C^CCGGG XmnI 125 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccIII 195 TCCGGA T^CCGGA 392 TCCGGA AocI 859 CCTGAGG CC^T[ACGT]AGG 1327 CCTGAGG BanI 255 GGTGCC G^G[CT][GA]CC 717 GGTACC BclI 155 TGATCA T^GATCA 1397 TGATCA BsaWI 195 TCCGGA [AT]^CCGG[AT] 392 TCCGGA BseAI 195 TCCGGA T^CCGGA 392 TCCGGA BseRI 1123 GAGGAG GAGGAG(10/8) 1222 GAGGAG BsgI 1145 CTGCAC GTGCAG(16/14) 942 GTGCAG BsmFI 271 GGGAC GGGAC(10/14) 69 GTCCC BspEI 195 TCCGGA T^CCGGA 392 TCCGGA BspMI 1295 ACCTGC ACCTGC(4/8) 1481 ACCTGC BsrDI 650 GCAATG GCAATG(2/0) 953 GCAATG BstAPI 944 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1251 GCAGAAGCTGC BstYI 219 AGATCC [GA]^GATC[CT] 854 GGATCC Bsu36I 859 CCTGAGG CC^T[ACGT]AGG 1327 CCTGAGG Eco81I 859 CCTGAGG CC^T[ACGT]AGG 1327 CCTGAGG EcoT14I 509 CCATGG C^C[AT][AT]GG 902 CCAAGG FbaI 155 TGATCA T^GATCA 1397 TGATCA MflI 219 AGATCC [GA]^GATC[CT] 854 GGATCC MroI 195 TCCGGA T^CCGGA 392 TCCGGA PleI 178 GACTC GAGTC(4/5) 1128 GAGTC PpuMI 518 GGGTCCT [GA]G^G[AT]CC[CT] 1490 AGGACCT PstI 1258 CTGCAG CTGCA^G 1465 CTGCAG ScaI 203 AGTACT AGT^ACT 1355 AGTACT SfaNI 439 GATGC GCATC(5/9) 542 GATGC SfcI 1258 CTGCAG C^T[GA][CT]AG 1465 CTGCAG SmlI 712 CTCAAG C^T[CT][GA]AG 1207 CTTGAG StuI 582 AGGCCT AGG^CCT 695 AGGCCT StyI 509 CCATGG C^C[AT][AT]GG 902 CCAAGG TaqI 123 TCGA T^CGA 593 TCGA TthHB8I 123 TCGA T^CGA 593 TCGA XhoII 219 AGATCC [GA]^GATC[CT] 854 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PvuII RsaI Sau3AI Sau96I ScrFI TfiI TseI Tsp45I Tsp509I TspRI XcmI