(Circular) (Six-base) MAPSORT of: pFN21AE1296.seq Check: 4759 from: 1 to: 6232 pFN21AE1296 6232 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3495 3495 Size: 6232 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4042 278 Size: 53 83 186 936 2506 2468 Fragments arranged by size: 2506 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3575 1937 Size: 1638 4594 Acc65I G'GTAC_C Cuts at: 2722 3553 2722 Size: 831 5401 AclI AA'CG_TT Cuts at: 1563 4359 4732 1563 Size: 2796 373 3063 Fragments arranged by size: 3063 2796 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2121 2331 4295 5679 5947 1632 Size: 489 210 1964 1384 268 1917 Fragments arranged by size: 1964 1917 1384 489 268 210 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6035 AflIII A'CryG_T Cuts at: 1203 2750 5131 6108 1203 Size: 1547 2381 977 1327 Fragments arranged by size: 2381 1547 1327 977 AgeI A'CCGG_T Cuts at: 5063 5063 Size: 6232 AhdI GACnn_n'nnGTC Cuts at: 4961 4961 Size: 6232 AleI CACnn'nnGTG Cuts at: 1166 2909 1166 Size: 1743 4489 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6232 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3266 3298 3266 Size: 32 6200 AlwNI CAG_nnn'CTG Cuts at: 2366 2955 3262 3499 3975 5547 2366 Size: 589 307 237 476 1572 3051 Fragments arranged by size: 3051 1572 589 476 307 237 ApaI G_GGCC'C Cuts at: 3169 3169 Size: 6232 ApaLI G'TGCA_C Cuts at: 2693 4289 5445 2693 Size: 1596 1156 3480 Fragments arranged by size: 3480 1596 1156 ApoI r'AATT_y Cuts at: 1493 2080 2474 2534 3541 3767 3919 6028 1493 Size: 587 394 60 1007 226 152 2109 1697 Fragments arranged by size: 2109 1697 1007 587 394 226 152 60 AseI AT'TA_AT Cuts at: 160 1032 4786 160 Size: 872 3754 1606 Fragments arranged by size: 3754 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 AvaI C'yCGr_G Cuts at: 1870 1943 2272 2375 3125 3557 1870 Size: 73 329 103 750 432 4545 Fragments arranged by size: 4545 750 432 329 103 73 BamHI G'GATC_C Cuts at: 2859 3562 3929 2859 Size: 703 367 5162 Fragments arranged by size: 5162 703 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2260 2722 3553 5008 618 Size: 325 217 627 473 462 831 1455 1842 Fragments arranged by size: 1842 1455 831 627 473 462 325 217 BanII G_rGCy'C Cuts at: 1391 2273 2901 3169 3201 3322 3551 1391 Size: 882 628 268 32 121 229 4072 Fragments arranged by size: 4072 882 628 268 229 121 32 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3059 928 Size: 971 1160 4101 Fragments arranged by size: 4101 1160 971 BbvCI CC'TCA_GC Cuts at: 2286 2286 Size: 6232 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4423 4457 1903 Size: 34 2486 34 3678 Fragments arranged by size: 3678 2486 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4124 5334 4124 Size: 1210 5022 BclI T'GATC_A Cuts at: 1548 2160 3402 1548 Size: 612 1242 4378 Fragments arranged by size: 4378 1242 612 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4843 136 Size: 107 122 71 631 605 3171 1525 Fragments arranged by size: 3171 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6227 6227 Size: 6232 BlpI GC'TnA_GC Cuts at: 3649 3649 Size: 6232 Bme1580I G_kGCm'C Cuts at: 1790 2697 2758 3169 4293 5449 1790 Size: 907 61 411 1124 1156 2573 Fragments arranged by size: 2573 1156 1124 907 411 61 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6232 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3963 4921 442 Size: 416 53 83 399 24 2546 958 1753 Fragments arranged by size: 2546 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6232 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2098 2251 3065 3215 3521 4892 1239 Size: 859 153 814 150 306 1371 2579 Fragments arranged by size: 2579 1371 859 814 306 153 150 Bpu10I CC'TnA_GC Cuts at: 2286 2286 Size: 6232 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2702 3233 4357 5222 5520 2702 Size: 531 1124 865 298 3414 Fragments arranged by size: 3414 1124 865 531 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4895 882 Size: 617 3396 2219 Fragments arranged by size: 3396 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4023 493 Size: 711 2819 2702 Fragments arranged by size: 2819 2702 711 BsaBI GATnn'nnATC Cuts at: 6226 6226 Size: 6232 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4039 4421 5056 275 Size: 53 83 186 936 2506 382 635 1451 Fragments arranged by size: 2506 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2200 2397 4665 5063 5337 5484 5895 2200 Size: 197 2268 398 274 147 411 2537 Fragments arranged by size: 2537 2268 411 398 274 197 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3266 3296 1178 Size: 30 165 30 1863 30 4114 Fragments arranged by size: 4114 1863 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3143 3242 1186 Size: 1957 99 4176 Fragments arranged by size: 4176 1957 99 BseYI C'CCAG_C Cuts at: 2024 2091 2924 5435 5931 2024 Size: 67 833 2511 496 2325 Fragments arranged by size: 2511 2325 833 496 67 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2968 3135 1163 Size: 573 1232 167 4260 Fragments arranged by size: 4260 1232 573 167 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3682 4443 5471 1540 Size: 275 186 1681 761 1028 2301 Fragments arranged by size: 2301 1681 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2697 2854 2990 4293 4378 5449 2697 Size: 157 136 1303 85 1071 3480 Fragments arranged by size: 3480 1303 1071 157 136 85 BsmI GAATG_Cn' Cuts at: 2496 3743 3836 2496 Size: 1247 93 4892 Fragments arranged by size: 4892 1247 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2273 2697 2758 2854 2901 2990 3169 Size: 399 483 424 61 96 47 89 179 Cuts at: 3169 3201 3322 3551 4293 4378 5449 1391 Size: 32 121 229 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 483 424 399 229 179 121 96 89 85 61 47 32 BspEI T'CCGG_A Cuts at: 2200 2397 5895 2200 Size: 197 3498 2537 Fragments arranged by size: 3498 2537 197 BspHI T'CATG_A Cuts at: 4121 5851 4121 Size: 1730 4502 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3309 3495 3587 844 Size: 2465 186 92 3489 Fragments arranged by size: 3489 2465 186 92 BsrBI CCG'CTC Cuts at: 1394 1823 4119 1394 Size: 429 2296 3507 Fragments arranged by size: 3507 2296 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2662 2965 4727 4901 65 Size: 1183 1414 303 1762 174 1396 Fragments arranged by size: 1762 1414 1396 1183 303 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2931 4876 5063 1700 Size: 110 1121 1945 187 2869 Fragments arranged by size: 2869 1945 1121 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6232 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6232 BssSI C'ACGA_G Cuts at: 4292 5304 4292 Size: 1012 5220 BstAPI GCAn_nnn'nTGC Cuts at: 794 2955 3262 794 Size: 2161 307 3764 Fragments arranged by size: 3764 2161 307 BstBI TT'CG_AA Cuts at: 5036 5036 Size: 6232 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6232 BstYI r'GATC_y Cuts at: 1972 2224 2859 3562 3929 4314 4331 5783 5869 Size: 252 635 703 367 385 17 1452 86 Cuts at: 5869 5881 6227 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 703 635 385 367 346 252 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2865 3333 2865 Size: 468 5764 BtgI C'CryG_G Cuts at: 513 1065 2514 513 Size: 552 1449 4231 Fragments arranged by size: 4231 1449 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2560 487 Size: 683 56 1334 4159 Fragments arranged by size: 4159 1334 683 56 BtsI GCAGTG_nn' Cuts at: 2038 2163 3748 4542 4562 2038 Size: 125 1585 794 20 3708 Fragments arranged by size: 3708 1585 794 125 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6232 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6197 DraI TTT'AAA Cuts at: 3536 3536 Size: 6232 DraIII CAC_nnn'GTG Cuts at: 3241 3241 Size: 6232 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5239 809 Size: 507 3923 1802 Fragments arranged by size: 3923 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2295 3679 4568 Size: 54 1425 50 275 18 465 1384 889 Cuts at: 4568 6197 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1384 889 465 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3679 1537 Size: 275 1867 4090 Fragments arranged by size: 4090 1867 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2307 3368 3417 4162 1353 Size: 954 1061 49 745 3423 Fragments arranged by size: 3423 1061 954 745 49 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4819 5193 5339 387 Size: 1064 138 3230 374 146 1280 Fragments arranged by size: 3230 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2098 2121 2251 2331 3065 3215 3521 Size: 393 466 23 130 80 734 150 306 Cuts at: 3521 4295 4892 5679 5947 1239 Size: 774 597 787 268 1524 Fragments arranged by size: 1524 787 774 734 597 466 393 306 268 150 130 80 23 EcoNI CCTnn'n_nnAGG Cuts at: 3491 3491 Size: 6232 EcoO109I rG'GnC_Cy Cuts at: 2524 2973 3040 3165 3166 3496 2524 Size: 449 67 125 1 330 5260 Fragments arranged by size: 5260 449 330 125 67 1 EcoRI G'AATT_C Cuts at: 2080 3541 3919 2080 Size: 1461 378 4393 Fragments arranged by size: 4393 1461 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3076 3403 3076 Size: 327 5905 FspI TGC'GCA Cuts at: 4738 4738 Size: 6232 HaeII r_GCGC'y Cuts at: 1678 2942 5379 1678 Size: 1264 2437 2531 Fragments arranged by size: 2531 2437 1264 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2269 2301 4873 4905 4947 Size: 32 135 32 697 32 2572 32 42 Cuts at: 4947 4979 1373 Size: 32 2626 Fragments arranged by size: 2626 2572 697 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2064 3298 3385 3576 3822 669 Size: 988 281 126 1234 87 191 246 3079 Fragments arranged by size: 3079 1234 988 281 246 191 126 87 HindIII A'AGCT_T Cuts at: 748 748 Size: 6232 HpaI GTT'AAC Cuts at: 3822 3822 Size: 6232 KpnI G_GTAC'C Cuts at: 2726 3557 2726 Size: 831 5401 MfeI C'AATT_G Cuts at: 3831 3831 Size: 6232 MluI A'CGCG_T Cuts at: 6108 6108 Size: 6232 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3252 5346 5530 1305 Size: 1947 2094 184 2007 Fragments arranged by size: 2094 2007 1947 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2297 10 Size: 54 1425 343 465 3945 Fragments arranged by size: 3945 1425 465 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2168 2690 2909 4190 4549 4708 518 Size: 648 1002 522 219 1281 359 159 2042 Fragments arranged by size: 2042 1281 1002 648 522 359 219 159 MspA1I CmG'CkG Cuts at: 1606 2194 2736 3193 3475 3648 4325 5473 5718 Size: 588 542 457 282 173 677 1148 245 Cuts at: 5718 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 588 542 457 282 245 173 NcoI C'CATG_G Cuts at: 513 1065 2514 513 Size: 552 1449 4231 Fragments arranged by size: 4231 1449 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6232 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6232 NotI GC'GGCC_GC Cuts at: 3679 3679 Size: 6232 NspI r_CATG'y Cuts at: 1132 1229 2754 3590 5135 1132 Size: 97 1525 836 1545 2229 Fragments arranged by size: 2229 1545 1525 836 97 PasI CC'CwG_GG Cuts at: 1348 2758 1348 Size: 1410 4822 PciI A'CATG_T Cuts at: 2750 5131 2750 Size: 2381 3851 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6232 PmeI GTTT'AAAC Cuts at: 3536 3536 Size: 6232 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2651 2683 3266 3298 4301 4333 5814 5846 2651 Size: 32 583 32 1003 32 1481 32 3037 Fragments arranged by size: 3037 1481 1003 583 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2524 3496 2524 Size: 972 5260 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6232 PsiI TTA'TAA Cuts at: 3802 3802 Size: 6232 PspOMI G'GGCC_C Cuts at: 3165 3165 Size: 6232 PstI C_TGCA'G Cuts at: 830 3267 3474 3584 830 Size: 2437 207 110 3478 Fragments arranged by size: 3478 2437 207 110 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6232 PvuII CAG'CTG Cuts at: 2194 2736 3475 2194 Size: 542 739 4951 Fragments arranged by size: 4951 739 542 SalI G'TCGA_C Cuts at: 1936 3574 1936 Size: 1638 4594 SbfI CC_TGCA'GG Cuts at: 3584 3584 Size: 6232 ScaI AGT'ACT Cuts at: 2210 3362 4480 6192 2210 Size: 1152 1118 1712 2250 Fragments arranged by size: 2250 1712 1152 1118 SfcI C'TryA_G Cuts at: 826 1045 3263 3470 3580 4715 5396 5587 826 Size: 219 2218 207 110 1135 681 191 1471 Fragments arranged by size: 2218 1471 1135 681 219 207 191 110 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6232 SmaI CCC'GGG Cuts at: 1872 2274 3559 1872 Size: 402 1285 4545 Fragments arranged by size: 4545 1285 402 SmlI C'TyrA_G Cuts at: 820 1017 1943 2717 3212 4336 5237 5499 820 Size: 197 926 774 495 1124 901 262 1553 Fragments arranged by size: 1553 1124 926 901 774 495 262 197 SnaBI TAC'GTA Cuts at: 493 4023 493 Size: 3530 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6232 SphI G_CATG'C Cuts at: 1132 3590 1132 Size: 2458 3774 SspI AAT'ATT Cuts at: 5 52 4156 5 Size: 47 4104 2081 Fragments arranged by size: 4104 2081 47 StuI AGG'CCT Cuts at: 1517 2589 2702 1517 Size: 1072 113 5047 Fragments arranged by size: 5047 1072 113 StyI C'CwwG_G Cuts at: 513 1065 2514 2907 3671 513 Size: 552 1449 393 764 3074 Fragments arranged by size: 3074 1449 764 552 393 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4259 4276 4429 4614 5059 325 Size: 1255 2679 17 153 185 445 1498 Fragments arranged by size: 2679 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2208 2346 3360 Size: 274 80 33 51 1443 231 138 1014 Cuts at: 3360 4478 6190 96 Size: 1118 1712 138 Fragments arranged by size: 1712 1443 1118 1014 274 231 138 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6192 6224 6192 Size: 32 6200 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6232 XbaI T'CTAG_A Cuts at: 3568 3568 Size: 6232 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2639 2705 2733 2639 Size: 66 28 6138 Fragments arranged by size: 6138 66 28 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6232 XmaI C'CCGG_G Cuts at: 1870 2272 3557 1870 Size: 402 1285 4545 Fragments arranged by size: 4545 1285 402 XmnI GAAnn'nnTTC Cuts at: 2134 4361 2134 Size: 2227 4005 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4040 276 Size: 53 83 186 936 2506 2468 Fragments arranged by size: 2506 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI