Restriction map for
pFN21AE1296
(Linear) (Six-base) MAPSORT of: KIEE1296.seq Check: 9136 from: 1 to: 1527
KIEE1296.orf
With 182 enzymes: SgfI *
July 15, 2010 18:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1490 1527
Size: 1490 37
Acc65I G'GTAC_C
Cuts at: 0 717 1527
Size: 717 810
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 326 1527
Size: 116 210 1201
Fragments arranged by size:
1201 210 116
AflIII A'CryG_T
Cuts at: 0 745 1527
Size: 745 782
AleI CACnn'nnGTG
Cuts at: 0 904 1527
Size: 904 623
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1261 1293 1527
Size: 1261 32 234
Fragments arranged by size:
1261 234 32
AlwNI CAG_nnn'CTG
Cuts at: 0 361 950 1257 1494 1527
Size: 361 589 307 237 33
Fragments arranged by size:
589 361 307 237 33
ApaI G_GGCC'C
Cuts at: 0 1164 1527
Size: 1164 363
ApaLI G'TGCA_C
Cuts at: 0 688 1527
Size: 688 839
ApoI r'AATT_y
Cuts at: 0 75 469 529 1527
Size: 75 394 60 998
Fragments arranged by size:
998 394 75 60
AvaI C'yCGr_G
Cuts at: 0 267 370 1120 1527
Size: 267 103 750 407
Fragments arranged by size:
750 407 267 103
BamHI G'GATC_C
Cuts at: 0 854 1527
Size: 854 673
BanI G'GyrC_C
Cuts at: 0 255 717 1527
Size: 255 462 810
Fragments arranged by size:
810 462 255
BanII G_rGCy'C
Cuts at: 0 268 896 1164 1196 1317 1527
Size: 268 628 268 32 121 210
Fragments arranged by size:
628 268 268 210 121 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 1054 1527
Size: 1054 473
BbvCI CC'TCA_GC
Cuts at: 0 281 1527
Size: 281 1246
BclI T'GATC_A
Cuts at: 0 155 1397 1527
Size: 155 1242 130
Fragments arranged by size:
1242 155 130
Bme1580I G_kGCm'C
Cuts at: 0 692 753 1164 1527
Size: 692 61 411 363
Fragments arranged by size:
692 411 363 61
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 246 1060 1210 1516 1527
Size: 93 153 814 150 306 11
Fragments arranged by size:
814 306 153 150 93 11
Bpu10I CC'TnA_GC
Cuts at: 0 281 1527
Size: 281 1246
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 1228 1527
Size: 697 531 299
Fragments arranged by size:
697 531 299
BsaWI w'CCGG_w
Cuts at: 0 195 392 1527
Size: 195 197 1135
Fragments arranged by size:
1135 197 195
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1261 1291 1527
Size: 1261 30 236
Fragments arranged by size:
1261 236 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1138 1237 1527
Size: 1138 99 290
Fragments arranged by size:
1138 290 99
BseYI C'CCAG_C
Cuts at: 0 19 86 919 1527
Size: 19 67 833 608
Fragments arranged by size:
833 608 67 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 963 1130 1527
Size: 963 167 397
Fragments arranged by size:
963 397 167
BsiHKAI G_wGCw'C
Cuts at: 0 692 849 985 1527
Size: 692 157 136 542
Fragments arranged by size:
692 542 157 136
BsmI GAATG_Cn'
Cuts at: 0 491 1527
Size: 491 1036
Bsp1286I G_dGCh'C
Cuts at: 0 268 692 753 849 896 985 1164 1196
Size: 268 424 61 96 47 89 179 32
Cuts at: 1196 1317 1527
Size: 121 210
Fragments arranged by size:
424 268 210 179 121 96 89 61
47 32
BspEI T'CCGG_A
Cuts at: 0 195 392 1527
Size: 195 197 1135
Fragments arranged by size:
1135 197 195
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1304 1490 1527
Size: 1304 186 37
Fragments arranged by size:
1304 186 37
BsrDI GCAATG_nn'
Cuts at: 0 657 960 1527
Size: 657 303 567
Fragments arranged by size:
657 567 303
BsrFI r'CCGG_y
Cuts at: 0 926 1527
Size: 926 601
BstAPI GCAn_nnn'nTGC
Cuts at: 0 950 1257 1527
Size: 950 307 270
Fragments arranged by size:
950 307 270
BstYI r'GATC_y
Cuts at: 0 219 854 1527
Size: 219 635 673
Fragments arranged by size:
673 635 219
Bsu36I CC'TnA_GG
Cuts at: 0 860 1328 1527
Size: 860 468 199
Fragments arranged by size:
860 468 199
BtgI C'CryG_G
Cuts at: 0 509 1527
Size: 509 1018
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 555 1527
Size: 555 972
BtsI GCAGTG_nn'
Cuts at: 0 33 158 1527
Size: 33 125 1369
Fragments arranged by size:
1369 125 33
DraIII CAC_nnn'GTG
Cuts at: 0 1236 1527
Size: 1236 291
EaeI y'GGCC_r
Cuts at: 0 290 1527
Size: 290 1237
EarI CTCTTCn'nnn_
Cuts at: 0 302 1363 1412 1527
Size: 302 1061 49 115
Fragments arranged by size:
1061 302 115 49
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 116 246 326 1060 1210 1516 1527
Size: 93 23 130 80 734 150 306 11
Fragments arranged by size:
734 306 150 130 93 80 23 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1486 1527
Size: 1486 41
EcoO109I rG'GnC_Cy
Cuts at: 0 519 968 1035 1160 1161 1491 1527
Size: 519 449 67 125 1 330 36
Fragments arranged by size:
519 449 330 125 67 36 1
EcoRI G'AATT_C
Cuts at: 0 75 1527
Size: 75 1452
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1071 1398 1527
Size: 1071 327 129
Fragments arranged by size:
1071 327 129
HaeII r_GCGC'y
Cuts at: 0 937 1527
Size: 937 590
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 264 296 1527
Size: 264 32 1231
Fragments arranged by size:
1231 264 32
HincII GTy'rAC
Cuts at: 0 59 1293 1380 1527
Size: 59 1234 87 147
Fragments arranged by size:
1234 147 87 59
KpnI G_GTAC'C
Cuts at: 0 721 1527
Size: 721 806
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1247 1527
Size: 1247 280
MscI TGG'CCA
Cuts at: 0 292 1527
Size: 292 1235
MslI CAynn'nnrTG
Cuts at: 0 163 685 904 1527
Size: 163 522 219 623
Fragments arranged by size:
623 522 219 163
MspA1I CmG'CkG
Cuts at: 0 189 731 1188 1470 1527
Size: 189 542 457 282 57
Fragments arranged by size:
542 457 282 189 57
NcoI C'CATG_G
Cuts at: 0 509 1527
Size: 509 1018
NspI r_CATG'y
Cuts at: 0 749 1527
Size: 749 778
PasI CC'CwG_GG
Cuts at: 0 753 1527
Size: 753 774
PciI A'CATG_T
Cuts at: 0 745 1527
Size: 745 782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 646 678 1261 1293 1527
Size: 646 32 583 32 234
Fragments arranged by size:
646 583 234 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 519 1491 1527
Size: 519 972 36
Fragments arranged by size:
972 519 36
PspOMI G'GGCC_C
Cuts at: 0 1160 1527
Size: 1160 367
PstI C_TGCA'G
Cuts at: 0 1262 1469 1527
Size: 1262 207 58
Fragments arranged by size:
1262 207 58
PvuII CAG'CTG
Cuts at: 0 189 731 1470 1527
Size: 189 542 739 57
Fragments arranged by size:
739 542 189 57
ScaI AGT'ACT
Cuts at: 0 205 1357 1527
Size: 205 1152 170
Fragments arranged by size:
1152 205 170
SfcI C'TryA_G
Cuts at: 0 1258 1465 1527
Size: 1258 207 62
Fragments arranged by size:
1258 207 62
SmaI CCC'GGG
Cuts at: 0 269 1527
Size: 269 1258
SmlI C'TyrA_G
Cuts at: 0 712 1207 1527
Size: 712 495 320
Fragments arranged by size:
712 495 320
StuI AGG'CCT
Cuts at: 0 584 697 1527
Size: 584 113 830
Fragments arranged by size:
830 584 113
StyI C'CwwG_G
Cuts at: 0 509 902 1527
Size: 509 393 625
Fragments arranged by size:
625 509 393
TatI w'GTAC_w
Cuts at: 0 203 341 1355 1527
Size: 203 138 1014 172
Fragments arranged by size:
1014 203 172 138
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 634 700 728 1527
Size: 634 66 28 799
Fragments arranged by size:
799 634 66 28
XmaI C'CCGG_G
Cuts at: 0 267 1527
Size: 267 1260
XmnI GAAnn'nnTTC
Cuts at: 0 129 1527
Size: 129 1398
Enzymes that do cut:
AarI Acc65I AcuI AflIII AleI AloI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI
BclI Bme1580I BpmI Bpu10I BpuEI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrDI
BsrFI BstAPI BstYI Bsu36I BtgI BtgZI BtsI DraIII
EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
Hin4I HincII KpnI MmeI MscI MslI MspA1I NcoI
NspI PasI PciI PpiI PpuMI PspOMI PstI PvuII
ScaI SfcI SmaI SmlI StuI StyI TatI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AlfI
AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
BsaI BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BspHI
BsrBI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I
ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoRV
FseI FspI FspAI HindIII HpaI KasI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PflMI PfoI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TstI Tth111I XbaI
XhoI ZraI