(Circular) (Six-base) MAPSORT of: pFN21AB0481.seq Check: 7340 from: 1 to: 6268 pFN21AB0481 6268 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2999 4078 278 Size: 53 83 186 936 1463 1079 2468 Fragments arranged by size: 2468 1463 1079 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3611 1937 Size: 1674 4594 Acc65I G'GTAC_C Cuts at: 3589 3589 Size: 6268 AclI AA'CG_TT Cuts at: 1563 4395 4768 1563 Size: 2832 373 3063 Fragments arranged by size: 3063 2832 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2337 3062 3436 4331 5715 5983 1632 Size: 705 725 374 895 1384 268 1917 Fragments arranged by size: 1917 1384 895 725 705 374 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6071 AflIII A'CryG_T Cuts at: 1203 5167 6144 1203 Size: 3964 977 1327 Fragments arranged by size: 3964 1327 977 AgeI A'CCGG_T Cuts at: 5099 5099 Size: 6268 AhdI GACnn_n'nnGTC Cuts at: 2995 4997 2995 Size: 2002 4266 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6268 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6268 AlwNI CAG_nnn'CTG Cuts at: 3391 4011 5583 3391 Size: 620 1572 4076 Fragments arranged by size: 4076 1572 620 ApaI G_GGCC'C Cuts at: 2351 2351 Size: 6268 ApaLI G'TGCA_C Cuts at: 4325 5481 4325 Size: 1156 5112 ApoI r'AATT_y Cuts at: 1493 2245 3304 3577 3803 3955 6064 1493 Size: 752 1059 273 226 152 2109 1697 Fragments arranged by size: 2109 1697 1059 752 273 226 152 AseI AT'TA_AT Cuts at: 160 1032 4822 160 Size: 872 3790 1606 Fragments arranged by size: 3790 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6268 AvaI C'yCGr_G Cuts at: 1870 1943 2529 2587 2687 3593 1870 Size: 73 586 58 100 906 4545 Fragments arranged by size: 4545 906 586 100 73 58 BamHI G'GATC_C Cuts at: 3598 3965 3598 Size: 367 5901 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2098 3084 3485 3589 5044 Size: 325 217 627 311 986 401 104 1455 Cuts at: 5044 618 Size: 1842 Fragments arranged by size: 1842 1455 986 627 401 325 311 217 104 BanII G_rGCy'C Cuts at: 1391 2351 2576 3282 3587 1391 Size: 960 225 706 305 4072 Fragments arranged by size: 4072 960 706 305 225 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2012 928 Size: 971 113 5184 Fragments arranged by size: 5184 971 113 BbvCI CC'TCA_GC Cuts at: 3473 3473 Size: 6268 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4459 4493 1903 Size: 34 50 34 2438 34 3678 Fragments arranged by size: 3678 2438 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4160 5370 4160 Size: 1210 5058 BclI T'GATC_A Cuts at: 1548 1548 Size: 6268 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4879 136 Size: 107 122 71 631 605 337 2870 1525 Fragments arranged by size: 2870 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6263 6263 Size: 6268 BlpI GC'TnA_GC Cuts at: 3326 3408 3685 3326 Size: 82 277 5909 Fragments arranged by size: 5909 277 82 Bme1580I G_kGCm'C Cuts at: 1790 2071 2103 2216 2351 3488 4329 5485 1790 Size: 281 32 113 135 1137 841 1156 2573 Fragments arranged by size: 2573 1156 1137 841 281 135 113 32 BmgBI CAC'GTC Cuts at: 1318 2818 1318 Size: 1500 4768 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2869 3999 4957 Size: 416 53 83 399 24 1452 1130 958 Cuts at: 4957 442 Size: 1753 Fragments arranged by size: 1753 1452 1130 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6268 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2176 2176 Size: 6268 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2221 2469 2690 3464 4928 1239 Size: 982 248 221 774 1464 2579 Fragments arranged by size: 2579 1464 982 774 248 221 Bpu10I CC'TnA_GC Cuts at: 3473 3473 Size: 6268 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4393 5258 5556 4393 Size: 865 298 5105 Fragments arranged by size: 5105 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2401 4931 882 Size: 617 902 2530 2219 Fragments arranged by size: 2530 2219 902 617 BsaAI yAC'GTr Cuts at: 493 1204 4059 493 Size: 711 2855 2702 Fragments arranged by size: 2855 2702 711 BsaBI GATnn'nnATC Cuts at: 3422 6262 3422 Size: 2840 3428 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2089 2996 4075 4457 Size: 53 83 186 936 556 907 1079 382 Cuts at: 4457 5092 275 Size: 635 1451 Fragments arranged by size: 1451 1079 936 907 635 556 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2576 3354 4701 5099 5373 5520 5931 2576 Size: 778 1347 398 274 147 411 2913 Fragments arranged by size: 2913 1347 778 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3158 3188 1178 Size: 30 165 30 1755 30 4258 Fragments arranged by size: 4258 1755 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3122 1186 Size: 1936 4332 BseYI C'CCAG_C Cuts at: 2083 2694 3372 3561 5471 5967 2083 Size: 611 678 189 1910 496 2384 Fragments arranged by size: 2384 1910 678 611 496 189 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2534 3068 1163 Size: 573 798 534 4363 Fragments arranged by size: 4363 798 573 534 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3718 4479 5507 1540 Size: 275 186 1717 761 1028 2301 Fragments arranged by size: 2301 1717 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4329 4414 5485 4329 Size: 85 1071 5112 Fragments arranged by size: 5112 1071 85 BsmI GAATG_Cn' Cuts at: 3779 3872 3779 Size: 93 6175 BsmBI CGTCTCn'nnnn_ Cuts at: 2096 2201 2096 Size: 105 6163 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2071 2103 2216 2351 2576 3282 3488 Size: 399 281 32 113 135 225 706 206 Cuts at: 3488 3587 4329 4414 5485 1391 Size: 99 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 706 399 281 225 206 135 113 99 85 32 BspEI T'CCGG_A Cuts at: 2576 3354 5931 2576 Size: 778 2577 2913 Fragments arranged by size: 2913 2577 778 BspHI T'CATG_A Cuts at: 4157 5887 4157 Size: 1730 4538 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3556 3623 844 Size: 2712 67 3489 Fragments arranged by size: 3489 2712 67 BsrBI CCG'CTC Cuts at: 1394 1823 4155 1394 Size: 429 2332 3507 Fragments arranged by size: 3507 2332 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4763 4937 65 Size: 1183 3515 174 1396 Fragments arranged by size: 3515 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4912 5099 1700 Size: 110 3102 187 2869 Fragments arranged by size: 3102 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6268 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6268 BssSI C'ACGA_G Cuts at: 4328 5340 4328 Size: 1012 5256 BstAPI GCAn_nnn'nTGC Cuts at: 794 3055 794 Size: 2261 4007 BstBI TT'CG_AA Cuts at: 5072 5072 Size: 6268 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6268 BstXI CCAn_nnnn'nTGG Cuts at: 2258 3244 3321 2258 Size: 986 77 5205 Fragments arranged by size: 5205 986 77 BstYI r'GATC_y Cuts at: 1972 2154 3598 3965 4350 4367 5819 5905 5917 Size: 182 1444 367 385 17 1452 86 12 Cuts at: 5917 6263 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1444 385 367 346 182 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2899 513 Size: 552 1834 3882 Fragments arranged by size: 3882 1834 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3492 487 Size: 683 56 2266 3263 Fragments arranged by size: 3263 2266 683 56 BtsI GCAGTG_nn' Cuts at: 2529 3017 3392 3784 4578 4598 2529 Size: 488 375 392 794 20 4199 Fragments arranged by size: 4199 794 488 392 375 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6268 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6233 DraI TTT'AAA Cuts at: 3572 3572 Size: 6268 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5275 809 Size: 507 3959 1802 Fragments arranged by size: 3959 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 3105 3255 Size: 54 1425 50 275 18 180 1095 150 Cuts at: 3255 3715 4604 6233 8 Size: 460 889 1629 43 Fragments arranged by size: 1629 1425 1095 889 460 275 180 150 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3715 1537 Size: 275 1903 4090 Fragments arranged by size: 4090 1903 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4198 1353 Size: 2845 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3415 4855 5229 5375 387 Size: 1064 138 1826 1440 374 146 1280 Fragments arranged by size: 1826 1440 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2221 2337 2469 2690 3062 3436 3464 Size: 393 589 116 132 221 372 374 28 Cuts at: 3464 4331 4928 5715 5983 1239 Size: 867 597 787 268 1524 Fragments arranged by size: 1524 867 787 597 589 393 374 372 268 221 132 116 28 EcoNI CCTnn'n_nnAGG Cuts at: 2468 2468 Size: 6268 EcoO109I rG'GnC_Cy Cuts at: 2347 2348 2347 Size: 1 6267 EcoRI G'AATT_C Cuts at: 3577 3955 3577 Size: 378 5890 EcoRV GAT'ATC Cuts at: 3194 3194 Size: 6268 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2246 2956 2246 Size: 710 5558 FspI TGC'GCA Cuts at: 4774 4774 Size: 6268 HaeII r_GCGC'y Cuts at: 1678 2659 5415 1678 Size: 981 2756 2531 Fragments arranged by size: 2756 2531 981 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2106 2138 2146 2178 2355 Size: 32 135 32 534 32 8 32 177 Cuts at: 2355 2387 2510 2542 2981 3013 3387 3419 3447 Size: 32 123 32 439 32 374 32 28 Cuts at: 3447 3479 4909 4941 4983 5015 1373 Size: 32 1430 32 42 32 2626 Fragments arranged by size: 2626 1430 534 439 374 177 135 123 42 32 32 32 32 32 32 32 32 32 32 32 28 8 HincII GTy'rAC Cuts at: 669 1657 1938 3612 3858 669 Size: 988 281 1674 246 3079 Fragments arranged by size: 3079 1674 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6268 HpaI GTT'AAC Cuts at: 3858 3858 Size: 6268 KpnI G_GTAC'C Cuts at: 3593 3593 Size: 6268 MfeI C'AATT_G Cuts at: 3867 3867 Size: 6268 MluI A'CGCG_T Cuts at: 6144 6144 Size: 6268 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2496 3490 5382 5566 1305 Size: 1191 994 1892 184 2007 Fragments arranged by size: 2007 1892 1191 994 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2012 3107 3257 10 Size: 54 1425 343 180 1095 150 3021 Fragments arranged by size: 3021 1425 1095 343 180 150 54 MslI CAynn'nnrTG Cuts at: 518 1166 2969 3021 3176 3437 3506 4226 4585 Size: 648 1803 52 155 261 69 720 359 Cuts at: 4585 4744 518 Size: 159 2042 Fragments arranged by size: 2042 1803 720 648 359 261 159 155 69 52 MspA1I CmG'CkG Cuts at: 1606 3121 3684 4361 5509 5754 1606 Size: 1515 563 677 1148 245 2120 Fragments arranged by size: 2120 1515 1148 677 563 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5716 NdeI CA'TA_TG Cuts at: 387 387 Size: 6268 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6268 NotI GC'GGCC_GC Cuts at: 3715 3715 Size: 6268 NspI r_CATG'y Cuts at: 1132 1229 3626 5171 1132 Size: 97 2397 1545 2229 Fragments arranged by size: 2397 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2073 2379 3094 1348 Size: 725 306 715 4522 Fragments arranged by size: 4522 725 715 306 PciI A'CATG_T Cuts at: 5167 5167 Size: 6268 PflMI CCAn_nnn'nTGG Cuts at: 1108 2790 1108 Size: 1682 4586 PmeI GTTT'AAAC Cuts at: 3572 3572 Size: 6268 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2352 2384 4337 4369 5850 5882 2352 Size: 32 1953 32 1481 32 2738 Fragments arranged by size: 2738 1953 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6268 PsiI TTA'TAA Cuts at: 3838 3838 Size: 6268 PspOMI G'GGCC_C Cuts at: 2347 2347 Size: 6268 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3332 3364 3332 Size: 32 6236 PstI C_TGCA'G Cuts at: 830 3012 3189 3620 830 Size: 2182 177 431 3478 Fragments arranged by size: 3478 2182 431 177 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6268 PvuII CAG'CTG Cuts at: 3121 3121 Size: 6268 SalI G'TCGA_C Cuts at: 1936 3610 1936 Size: 1674 4594 SbfI CC_TGCA'GG Cuts at: 3620 3620 Size: 6268 ScaI AGT'ACT Cuts at: 4516 6228 4516 Size: 1712 4556 SfcI C'TryA_G Cuts at: 826 1045 2911 3008 3116 3185 3616 4751 5432 Size: 219 1866 97 108 69 431 1135 681 Cuts at: 5432 5623 826 Size: 191 1471 Fragments arranged by size: 1866 1471 1135 681 431 219 191 108 97 69 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6268 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6268 SmaI CCC'GGG Cuts at: 1872 3595 1872 Size: 1723 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4372 5273 5535 820 Size: 197 926 2429 901 262 1553 Fragments arranged by size: 2429 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4059 493 Size: 3566 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6268 SphI G_CATG'C Cuts at: 1132 3626 1132 Size: 2494 3774 SspI AAT'ATT Cuts at: 5 52 4192 5 Size: 47 4140 2081 Fragments arranged by size: 4140 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6268 StyI C'CwwG_G Cuts at: 513 1065 2013 3707 513 Size: 552 948 1694 3074 Fragments arranged by size: 3074 1694 948 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2861 4295 4312 4465 4650 5095 325 Size: 1255 1281 1434 17 153 185 445 1498 Fragments arranged by size: 1498 1434 1281 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4514 6226 96 Size: 274 80 33 51 1443 2537 1712 138 Fragments arranged by size: 2537 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2019 2051 2119 2151 2208 2240 3158 3190 6228 Size: 32 68 32 57 32 918 32 3038 Cuts at: 6228 6260 2019 Size: 32 2027 Fragments arranged by size: 3038 2027 918 68 57 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2537 1316 Size: 1221 5047 XbaI T'CTAG_A Cuts at: 3604 3604 Size: 6268 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2080 2897 3265 2080 Size: 817 368 5083 Fragments arranged by size: 5083 817 368 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6268 XmaI C'CCGG_G Cuts at: 1870 3593 1870 Size: 1723 4545 XmnI GAAnn'nnTTC Cuts at: 4397 4397 Size: 6268 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2997 4076 276 Size: 53 83 186 936 1463 1079 2468 Fragments arranged by size: 2468 1463 1079 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI