################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BcgI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstXI Bsu36I CelII ClaI CpoI DraII DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV FseI FspI HaeII HindIII HpaI KasI KpnI Ksp632I KspI MaeII MamI MluI MroI MslI MvnI NarI NciI NlaIV NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### BbsI 385 GAAGAC GAAGAC(2/6) BbvCI 129 CCTCAGC CCTCAGC(-5/-2) BciVI 1447 GGATAC GTATCC(6/5) BclI 1151 TGATCA T^GATCA BpmI 1205 CTCCAG CTGGAG(16/14) BpuAI 385 GAAGAC GAAGAC(2/6) BseRI 1175 CTCCTC GAGGAG(10/8) BsgI 359 CTGCAC GTGCAG(16/14) BsmFI 776 GGGAC GGGAC(10/14) BsmI 393 GAATGC GAATGC(1/-1) BspHI 1154 TCATGA T^CATGA BsrFI 160 GCCGGC [GA]^CCGG[CT] Bst1107I 1194 GTATAC GTA^TAC BstYI 1092 GGATCT [GA]^GATC[CT] BstZ17I 1194 GTATAC GTA^TAC CfoI 119 GCGC GCG^C Cfr10I 160 GCCGGC [GA]^CCGG[CT] DraI 520 TTTAAA TTT^AAA DrdI 1398 GACAATTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 314 CCATGG C^C[GA][CT]GG EcoT22I 278 ATGCAT ATGCA^T FbaI 1151 TGATCA T^GATCA HapII 161 CCGG C^CGG HhaI 119 GCGC GCG^C HinP1I 119 GCGC G^CGC HincII 668 GTCAAC GT[CT]^[GA]AC HindII 668 GTCAAC GT[CT]^[GA]AC HpaII 161 CCGG C^CGG MfeI 260 CAATTG C^AATTG MflI 1092 GGATCT [GA]^GATC[CT] MspI 161 CCGG C^CGG MunI 260 CAATTG C^AATTG NaeI 160 GCCGGC GCC^GGC NcoI 314 CCATGG C^CATGG NdeI 281 CATATG CA^TATG NgoMIV 160 GCCGGC G^CCGGC NheI 634 GCTAGC G^CTAGC NsiI 278 ATGCAT ATGCA^T PleI 1255 GACTC GAGTC(4/5) PstI 37 CTGCAG CTGCA^G RcaI 1154 TCATGA T^CATGA SpeI 973 ACTAGT A^CTAGT SphI 234 GCATGC GCATG^C Tsp45I 79 GTGAC ^GT[GC]AC XbaI 270 TCTAGA T^CTAGA XcmI 639 CCATTGTTTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1092 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcyI 896 GGCGTC G[GA]^CG[CT]C 1352 GGCGTC AlwI 147 GATCC GGATC(4/5) 1092 GGATC AspHI 616 GAGCTC G[AT]GC[AT]^C 782 GAGCTC BalI 998 TGGCCA TGG^CCA 1166 TGGCCA BanII 616 GAGCTC G[GA]GC[CT]^C 782 GAGCTC BmyI 616 GAGCTC G[AGT]GC[ACT]^C 782 GAGCTC BsaHI 896 GGCGTC G[GA]^CG[CT]C 1352 GGCGTC BsaJI 228 CCTTGG C^C[ACGT][ACGT]GG 314 CCATGG BsiHKAI 616 GAGCTC G[AT]GC[AT]^C 782 GAGCTC BsmAI 1004 GAGAC GTCTC(1/5) 837 GTCTC Bsp1286I 616 GAGCTC G[AGT]GC[ACT]^C 782 GAGCTC BsrI 466 CCAGT ACTGG(1/-1) 1498 CCAGT EcoT14I 228 CCTTGG C^C[AT][AT]GG 314 CCATGG FauI 181 CCCGC CCCGC(4/6) 43 GCGGG Hin1I 896 GGCGTC G[GA]^CG[CT]C 1352 GGCGTC MaeIII 79 GTGAC ^GT[ACGT]AC 1508 GTTAC MluNI 998 TGGCCA TGG^CCA 1166 TGGCCA MscI 998 TGGCCA TGG^CCA 1166 TGGCCA MspA1I 175 CAGCTG C[AC]G^C[GT]G 691 CAGCTG NspI 234 GCATGC [GA]CATG^[CT] 528 GCATGT PflMI 63 CCAGGCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 223 CCATTCCTTGG PvuII 175 CAGCTG CAG^CTG 691 CAGCTG SacI 616 GAGCTC GAGCT^C 782 GAGCTC SexAI 167 ACCTGGT A^CC[AT]GGT 741 ACCAGGT SfcI 37 CTGCAG C^T[GA][CT]AG 408 CTACAG SmlI 839 CTCAAG C^T[CT][GA]AG 1087 CTCAAG StyI 228 CCTTGG C^C[AT][AT]GG 314 CCATGG TfiI 1059 GAATC G^A[AT]TC 1479 GATTC Van91I 63 CCAGGCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 223 CCATTCCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AccI AciI AcsI AluI ApoI AvaII BbvI BfaI BsiYI BslI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HgaI HinfI HphI ItaI MaeI MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII Sau3AI Sau96I ScrFI SfaNI Tru9I TseI Tsp509I TspRI