(Circular) (Six-base) MAPSORT of: pFN21AE1549.seq Check: 3090 from: 1 to: 6232 pFN21AE1549 6232 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4042 278 Size: 53 83 186 936 2506 2468 Fragments arranged by size: 2506 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2145 2412 3200 3575 1937 Size: 208 267 788 375 4594 Fragments arranged by size: 4594 788 375 267 208 Acc65I G'GTAC_C Cuts at: 3553 3553 Size: 6232 AclI AA'CG_TT Cuts at: 1563 4359 4732 1563 Size: 2796 373 3063 Fragments arranged by size: 3063 2796 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4295 5679 5947 1632 Size: 2663 1384 268 1917 Fragments arranged by size: 2663 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6035 AflIII A'CryG_T Cuts at: 1203 5131 6108 1203 Size: 3928 977 1327 Fragments arranged by size: 3928 1327 977 AgeI A'CCGG_T Cuts at: 5063 5063 Size: 6232 AhdI GACnn_n'nnGTC Cuts at: 4961 4961 Size: 6232 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6232 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6232 AlwNI CAG_nnn'CTG Cuts at: 3975 5547 3975 Size: 1572 4660 ApaLI G'TGCA_C Cuts at: 4289 5445 4289 Size: 1156 5076 ApoI r'AATT_y Cuts at: 1493 2270 3103 3379 3434 3541 3767 3919 6028 Size: 777 833 276 55 107 226 152 2109 Cuts at: 6028 1493 Size: 1697 Fragments arranged by size: 2109 1697 833 777 276 226 152 107 55 AseI AT'TA_AT Cuts at: 160 1032 4786 160 Size: 872 3754 1606 Fragments arranged by size: 3754 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 AvaI C'yCGr_G Cuts at: 1870 1943 3557 1870 Size: 73 1614 4545 Fragments arranged by size: 4545 1614 73 BamHI G'GATC_C Cuts at: 3562 3929 3562 Size: 367 5865 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3553 5008 618 Size: 325 217 627 1766 1455 1842 Fragments arranged by size: 1842 1766 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2625 2791 3551 1391 Size: 1234 166 760 4072 Fragments arranged by size: 4072 1234 760 166 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2397 928 Size: 971 498 4763 Fragments arranged by size: 4763 971 498 BbvCI CC'TCA_GC Cuts at: 2135 2135 Size: 6232 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4423 4457 1903 Size: 34 2486 34 3678 Fragments arranged by size: 3678 2486 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3446 4124 5334 3446 Size: 678 1210 4344 Fragments arranged by size: 4344 1210 678 BclI T'GATC_A Cuts at: 1548 3156 1548 Size: 1608 4624 BfrBI ATG'CAT Cuts at: 2285 2285 Size: 6232 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4843 136 Size: 107 122 71 631 605 337 2834 1525 Fragments arranged by size: 2834 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6227 6227 Size: 6232 BlpI GC'TnA_GC Cuts at: 3649 3649 Size: 6232 Bme1580I G_kGCm'C Cuts at: 1790 4293 5449 1790 Size: 2503 1156 2573 Fragments arranged by size: 2573 2503 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6232 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3497 3963 4921 Size: 416 53 83 399 24 2080 466 958 Cuts at: 4921 442 Size: 1753 Fragments arranged by size: 2080 1753 958 466 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2643 1056 Size: 1587 4645 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3195 4892 1239 Size: 1956 1697 2579 Fragments arranged by size: 2579 1956 1697 Bpu10I CC'TnA_GC Cuts at: 2135 2135 Size: 6232 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2829 3077 4357 5222 5520 2829 Size: 248 1280 865 298 3541 Fragments arranged by size: 3541 1280 865 298 248 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4895 882 Size: 617 3396 2219 Fragments arranged by size: 3396 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4023 493 Size: 711 2819 2702 Fragments arranged by size: 2819 2702 711 BsaBI GATnn'nnATC Cuts at: 6226 6226 Size: 6232 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2902 3358 4039 4421 Size: 53 83 186 936 1369 456 681 382 Cuts at: 4421 5056 275 Size: 635 1451 Fragments arranged by size: 1451 1369 936 681 635 456 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4665 5063 5337 5484 5895 4665 Size: 398 274 147 411 5002 Fragments arranged by size: 5002 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2201 2231 3193 3223 1178 Size: 30 165 30 798 30 962 30 4187 Fragments arranged by size: 4187 962 798 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3171 1186 Size: 1985 4247 BseYI C'CCAG_C Cuts at: 2018 2032 2062 3313 3353 5435 5931 2018 Size: 14 30 1251 40 2082 496 2319 Fragments arranged by size: 2319 2082 1251 496 40 30 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2349 1163 Size: 573 613 5046 Fragments arranged by size: 5046 613 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3682 4443 5471 1540 Size: 275 186 1681 761 1028 2301 Fragments arranged by size: 2301 1681 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2625 2791 4293 4378 5449 2625 Size: 166 1502 85 1071 3408 Fragments arranged by size: 3408 1502 1071 166 85 BsmI GAATG_Cn' Cuts at: 2404 3743 3836 2404 Size: 1339 93 4800 Fragments arranged by size: 4800 1339 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2625 2791 3551 4293 4378 5449 1391 Size: 399 835 166 760 742 85 1071 2174 Fragments arranged by size: 2174 1071 835 760 742 399 166 85 BspEI T'CCGG_A Cuts at: 5895 5895 Size: 6232 BspHI T'CATG_A Cuts at: 3159 4121 5851 3159 Size: 962 1730 3540 Fragments arranged by size: 3540 1730 962 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3587 844 Size: 2743 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4119 1394 Size: 429 2296 3507 Fragments arranged by size: 3507 2296 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4727 4901 65 Size: 1183 3479 174 1396 Fragments arranged by size: 3479 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2165 4876 5063 1700 Size: 110 355 2711 187 2869 Fragments arranged by size: 2869 2711 355 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6232 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6232 BssSI C'ACGA_G Cuts at: 4292 5304 4292 Size: 1012 5220 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6232 BstBI TT'CG_AA Cuts at: 5036 5036 Size: 6232 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6232 BstYI r'GATC_y Cuts at: 1972 3097 3562 3929 4314 4331 5783 5869 5881 Size: 1125 465 367 385 17 1452 86 12 Cuts at: 5881 6227 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1125 465 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3201 3201 Size: 6232 BtgI C'CryG_G Cuts at: 513 1065 2004 2319 513 Size: 552 939 315 4426 Fragments arranged by size: 4426 939 552 315 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2087 487 Size: 683 56 861 4632 Fragments arranged by size: 4632 861 683 56 BtsI GCAGTG_nn' Cuts at: 2366 3748 4542 4562 2366 Size: 1382 794 20 4036 Fragments arranged by size: 4036 1382 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6232 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6197 DraI TTT'AAA Cuts at: 2527 3536 2527 Size: 1009 5223 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3409 5239 809 Size: 507 2093 1830 1802 Fragments arranged by size: 2093 1830 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2160 2167 3003 Size: 54 1425 50 275 18 330 7 836 Cuts at: 3003 3171 3679 4568 6197 8 Size: 168 508 889 1629 43 Fragments arranged by size: 1629 1425 889 836 508 330 275 168 54 50 43 18 7 EagI C'GGCC_G Cuts at: 1537 1812 3679 1537 Size: 275 1867 4090 Fragments arranged by size: 4090 1867 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4162 1353 Size: 2809 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4819 5193 5339 387 Size: 1064 138 3230 374 146 1280 Fragments arranged by size: 3230 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2623 2789 2623 Size: 166 6066 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3195 4295 4892 5679 5947 1239 Size: 393 1563 1100 597 787 268 1524 Fragments arranged by size: 1563 1524 1100 787 597 393 268 EcoRI G'AATT_C Cuts at: 3541 3919 3541 Size: 378 5854 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2584 2584 Size: 6232 FspI TGC'GCA Cuts at: 4738 4738 Size: 6232 HaeII r_GCGC'y Cuts at: 1678 5379 1678 Size: 3701 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4873 4905 4947 4979 1373 Size: 32 135 32 3301 32 42 32 2626 Fragments arranged by size: 3301 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2675 3576 3822 669 Size: 988 281 737 901 246 3079 Fragments arranged by size: 3079 988 901 737 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6232 HpaI GTT'AAC Cuts at: 3822 3822 Size: 6232 KpnI G_GTAC'C Cuts at: 3557 3557 Size: 6232 MfeI C'AATT_G Cuts at: 2265 3831 2265 Size: 1566 4666 MluI A'CGCG_T Cuts at: 6108 6108 Size: 6232 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5346 5530 1305 Size: 4041 184 2007 Fragments arranged by size: 4041 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3005 3173 10 Size: 54 1425 343 1173 168 3069 Fragments arranged by size: 3069 1425 1173 343 168 54 MslI CAynn'nnrTG Cuts at: 518 1166 4190 4549 4708 518 Size: 648 3024 359 159 2042 Fragments arranged by size: 3024 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2182 2698 3648 4325 5473 5718 1606 Size: 576 516 950 677 1148 245 2120 Fragments arranged by size: 2120 1148 950 677 576 516 245 NaeI GCC'GGC Cuts at: 2167 2167 Size: 6232 NcoI C'CATG_G Cuts at: 513 1065 2004 2319 513 Size: 552 939 315 4426 Fragments arranged by size: 4426 939 552 315 NdeI CA'TA_TG Cuts at: 387 2287 387 Size: 1900 4332 NgoMIV G'CCGG_C Cuts at: 2165 2165 Size: 6232 NheI G'CTAG_C Cuts at: 1052 2639 1052 Size: 1587 4645 NotI GC'GGCC_GC Cuts at: 3679 3679 Size: 6232 NsiI A_TGCA'T Cuts at: 2287 2287 Size: 6232 NspI r_CATG'y Cuts at: 1132 1229 2243 2537 3590 5135 1132 Size: 97 1014 294 1053 1545 2229 Fragments arranged by size: 2229 1545 1053 1014 294 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6232 PciI A'CATG_T Cuts at: 5131 5131 Size: 6232 PflMI CCAn_nnn'nTGG Cuts at: 1108 2074 2234 1108 Size: 966 160 5106 Fragments arranged by size: 5106 966 160 PfoI T'CCnGG_A Cuts at: 3211 3211 Size: 6232 PmeI GTTT'AAAC Cuts at: 3536 3536 Size: 6232 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4301 4333 5814 5846 4301 Size: 32 1481 32 4687 Fragments arranged by size: 4687 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6232 PsiI TTA'TAA Cuts at: 3802 3802 Size: 6232 PstI C_TGCA'G Cuts at: 830 2046 3584 830 Size: 1216 1538 3478 Fragments arranged by size: 3478 1538 1216 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6232 PvuII CAG'CTG Cuts at: 2182 2698 2182 Size: 516 5716 SacI G_AGCT'C Cuts at: 2625 2791 2625 Size: 166 6066 SalI G'TCGA_C Cuts at: 1936 3574 1936 Size: 1638 4594 SbfI CC_TGCA'GG Cuts at: 3584 3584 Size: 6232 ScaI AGT'ACT Cuts at: 4480 6192 4480 Size: 1712 4520 SexAI A'CCwGG_T Cuts at: 2172 2746 2172 Size: 574 5658 SfcI C'TryA_G Cuts at: 826 1045 2042 2413 3580 4715 5396 5587 826 Size: 219 997 371 1167 1135 681 191 1471 Fragments arranged by size: 1471 1167 1135 997 681 371 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6232 SmaI CCC'GGG Cuts at: 1872 3559 1872 Size: 1687 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2844 3092 4336 5237 5499 820 Size: 197 926 901 248 1244 901 262 1553 Fragments arranged by size: 1553 1244 926 901 901 262 248 197 SnaBI TAC'GTA Cuts at: 493 4023 493 Size: 3530 2702 SpeI A'CTAG_T Cuts at: 152 2978 152 Size: 2826 3406 SphI G_CATG'C Cuts at: 1132 2243 3590 1132 Size: 1111 1347 3774 Fragments arranged by size: 3774 1347 1111 SspI AAT'ATT Cuts at: 5 52 4156 5 Size: 47 4104 2081 Fragments arranged by size: 4104 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6232 StyI C'CwwG_G Cuts at: 513 1065 2004 2233 2319 3671 513 Size: 552 939 229 86 1352 3074 Fragments arranged by size: 3074 1352 939 552 229 86 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4259 4276 4429 4614 5059 325 Size: 1255 2679 17 153 185 445 1498 Fragments arranged by size: 2679 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4478 6190 96 Size: 274 80 33 51 1443 2501 1712 138 Fragments arranged by size: 2501 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3207 3239 6192 6224 3207 Size: 32 2953 32 3215 Fragments arranged by size: 3215 2953 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6232 XbaI T'CTAG_A Cuts at: 2275 3568 2275 Size: 1293 4939 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2651 2651 Size: 6232 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6232 XmaI C'CCGG_G Cuts at: 1870 3557 1870 Size: 1687 4545 XmnI GAAnn'nnTTC Cuts at: 4361 4361 Size: 6232 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4040 276 Size: 53 83 186 936 2506 2468 Fragments arranged by size: 2506 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NarI NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI