(Linear) (Six-base) MAPSORT of: KIEE1549.seq Check: 7319 from: 1 to: 1527 KIEE1549.orf With 182 enzymes: SgfI * July 15, 2010 18:41 .. AccI GT'mk_AC Cuts at: 0 140 407 1195 1527 Size: 140 267 788 332 Fragments arranged by size: 788 332 267 140 ApoI r'AATT_y Cuts at: 0 265 1098 1374 1429 1527 Size: 265 833 276 55 98 Fragments arranged by size: 833 276 265 98 55 BanII G_rGCy'C Cuts at: 0 620 786 1527 Size: 620 166 741 Fragments arranged by size: 741 620 166 BbsI GAAGACnn'nnnn_ Cuts at: 0 392 1527 Size: 392 1135 BbvCI CC'TCA_GC Cuts at: 0 130 1527 Size: 130 1397 BciVI GTATCCnnnnn_n' Cuts at: 0 1441 1527 Size: 1441 86 BclI T'GATC_A Cuts at: 0 1151 1527 Size: 1151 376 BfrBI ATG'CAT Cuts at: 0 280 1527 Size: 280 1247 BmrI ACTGGGnnnn_n' Cuts at: 0 1492 1527 Size: 1492 35 BmtI G_CTAG'C Cuts at: 0 638 1527 Size: 638 889 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1190 1527 Size: 1190 337 Bpu10I CC'TnA_GC Cuts at: 0 130 1527 Size: 130 1397 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 824 1072 1527 Size: 824 248 455 Fragments arranged by size: 824 455 248 BsaHI Gr'CG_yC Cuts at: 0 897 1353 1527 Size: 897 456 174 Fragments arranged by size: 897 456 174 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 196 226 1188 1218 1527 Size: 196 30 962 30 309 Fragments arranged by size: 962 309 196 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1166 1527 Size: 1166 361 BseYI C'CCAG_C Cuts at: 0 13 27 57 1308 1348 1527 Size: 13 14 30 1251 40 179 Fragments arranged by size: 1251 179 40 30 14 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 344 1527 Size: 344 1183 BsiHKAI G_wGCw'C Cuts at: 0 620 786 1527 Size: 620 166 741 Fragments arranged by size: 741 620 166 BsmI GAATG_Cn' Cuts at: 0 399 1527 Size: 399 1128 Bsp1286I G_dGCh'C Cuts at: 0 620 786 1527 Size: 620 166 741 Fragments arranged by size: 741 620 166 BspHI T'CATG_A Cuts at: 0 1154 1527 Size: 1154 373 BsrFI r'CCGG_y Cuts at: 0 160 1527 Size: 160 1367 BstYI r'GATC_y Cuts at: 0 1092 1527 Size: 1092 435 BstZ17I GTA'TAC Cuts at: 0 1196 1527 Size: 1196 331 BtgI C'CryG_G Cuts at: 0 314 1527 Size: 314 1213 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 82 1527 Size: 82 1445 BtsI GCAGTG_nn' Cuts at: 0 361 1527 Size: 361 1166 DraI TTT'AAA Cuts at: 0 522 1527 Size: 522 1005 DrdI GACnn_nn'nnGTC Cuts at: 0 1404 1527 Size: 1404 123 EaeI y'GGCC_r Cuts at: 0 155 162 998 1166 1527 Size: 155 7 836 168 361 Fragments arranged by size: 836 361 168 155 7 EcoICRI GAG'CTC Cuts at: 0 618 784 1527 Size: 618 166 743 Fragments arranged by size: 743 618 166 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1190 1527 Size: 1190 337 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 579 1527 Size: 579 948 HincII GTy'rAC Cuts at: 0 670 1527 Size: 670 857 MfeI C'AATT_G Cuts at: 0 260 1527 Size: 260 1267 MscI TGG'CCA Cuts at: 0 1000 1168 1527 Size: 1000 168 359 Fragments arranged by size: 1000 359 168 MspA1I CmG'CkG Cuts at: 0 177 693 1527 Size: 177 516 834 Fragments arranged by size: 834 516 177 NaeI GCC'GGC Cuts at: 0 162 1527 Size: 162 1365 NcoI C'CATG_G Cuts at: 0 314 1527 Size: 314 1213 NdeI CA'TA_TG Cuts at: 0 282 1527 Size: 282 1245 NgoMIV G'CCGG_C Cuts at: 0 160 1527 Size: 160 1367 NheI G'CTAG_C Cuts at: 0 634 1527 Size: 634 893 NsiI A_TGCA'T Cuts at: 0 282 1527 Size: 282 1245 NspI r_CATG'y Cuts at: 0 238 532 1527 Size: 238 294 995 Fragments arranged by size: 995 294 238 PflMI CCAn_nnn'nTGG Cuts at: 0 69 229 1527 Size: 69 160 1298 Fragments arranged by size: 1298 160 69 PfoI T'CCnGG_A Cuts at: 0 1206 1527 Size: 1206 321 PstI C_TGCA'G Cuts at: 0 41 1527 Size: 41 1486 PvuII CAG'CTG Cuts at: 0 177 693 1527 Size: 177 516 834 Fragments arranged by size: 834 516 177 SacI G_AGCT'C Cuts at: 0 620 786 1527 Size: 620 166 741 Fragments arranged by size: 741 620 166 SexAI A'CCwGG_T Cuts at: 0 167 741 1527 Size: 167 574 786 Fragments arranged by size: 786 574 167 SfcI C'TryA_G Cuts at: 0 37 408 1527 Size: 37 371 1119 Fragments arranged by size: 1119 371 37 SmlI C'TyrA_G Cuts at: 0 839 1087 1527 Size: 839 248 440 Fragments arranged by size: 839 440 248 SpeI A'CTAG_T Cuts at: 0 973 1527 Size: 973 554 SphI G_CATG'C Cuts at: 0 238 1527 Size: 238 1289 StyI C'CwwG_G Cuts at: 0 228 314 1527 Size: 228 86 1213 Fragments arranged by size: 1213 228 86 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1202 1234 1527 Size: 1202 32 293 Fragments arranged by size: 1202 293 32 XbaI T'CTAG_A Cuts at: 0 270 1527 Size: 270 1257 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 646 1527 Size: 646 881 Enzymes that do cut: AccI ApoI BanII BbsI BbvCI BciVI BclI BfrBI BmrI BmtI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrFI BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI EcoICRI Eco57MI FalI HincII MfeI MscI MspA1I NaeI NcoI NdeI NgoMIV NheI NsiI NspI PflMI PfoI PstI PvuII SacI SexAI SfcI SmlI SpeI SphI StyI TstI XbaI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BcgI BglI BglII BlpI Bme1580I BmgBI BplI BsaI BsaAI BsaBI BsaWI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI Bsu36I ClaI CspCI DraIII EagI EarI EciI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII Hin4I HindIII HpaI KasI KpnI MluI MmeI MslI NarI NotI NruI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI SspI StuI SwaI TaqII TatI Tth111I XhoI XmaI XmnI ZraI