(Circular) (Six-base) MAPSORT of: pFN21AE1429.seq Check: 791 from: 1 to: 6313 pFN21AE1429 6313 bp With 182 enzymes: SgfI * June 19, 2019 18:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4123 278 Size: 53 83 186 936 2587 2468 Fragments arranged by size: 2587 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2214 3656 1937 Size: 277 1442 4594 Fragments arranged by size: 4594 1442 277 Acc65I G'GTAC_C Cuts at: 3634 3634 Size: 6313 AclI AA'CG_TT Cuts at: 1563 4440 4813 1563 Size: 2877 373 3063 Fragments arranged by size: 3063 2877 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2093 2192 4376 5760 6028 1632 Size: 461 99 2184 1384 268 1917 Fragments arranged by size: 2184 1917 1384 461 268 99 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6116 AflIII A'CryG_T Cuts at: 1203 3009 5212 6189 1203 Size: 1806 2203 977 1327 Fragments arranged by size: 2203 1806 1327 977 AgeI A'CCGG_T Cuts at: 5144 5144 Size: 6313 AhdI GACnn_n'nnGTC Cuts at: 3361 3541 5042 3361 Size: 180 1501 4632 Fragments arranged by size: 4632 1501 180 AleI CACnn'nnGTG Cuts at: 1166 2438 3251 1166 Size: 1272 813 4228 Fragments arranged by size: 4228 1272 813 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6313 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2402 2403 2434 2435 2402 Size: 1 31 1 6280 Fragments arranged by size: 6280 31 1 1 AlwNI CAG_nnn'CTG Cuts at: 2266 3343 4056 5628 2266 Size: 1077 713 1572 2951 Fragments arranged by size: 2951 1572 1077 713 ApaI G_GGCC'C Cuts at: 2447 2447 Size: 6313 ApaLI G'TGCA_C Cuts at: 2381 2738 4370 5526 2381 Size: 357 1632 1156 3168 Fragments arranged by size: 3168 1632 1156 357 ApoI r'AATT_y Cuts at: 1493 3176 3622 3848 4000 6109 1493 Size: 1683 446 226 152 2109 1697 Fragments arranged by size: 2109 1697 1683 446 226 152 AseI AT'TA_AT Cuts at: 160 1032 4867 160 Size: 872 3835 1606 Fragments arranged by size: 3835 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6313 AvaI C'yCGr_G Cuts at: 1870 1943 2331 3638 1870 Size: 73 388 1307 4545 Fragments arranged by size: 4545 1307 388 73 BamHI G'GATC_C Cuts at: 3643 4010 3643 Size: 367 5946 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3390 3634 5089 618 Size: 325 217 627 1603 244 1455 1842 Fragments arranged by size: 1842 1603 1455 627 325 244 217 BanII G_rGCy'C Cuts at: 1391 2447 2763 2980 3297 3632 1391 Size: 1056 316 217 317 335 4072 Fragments arranged by size: 4072 1056 335 317 316 217 BbeI G_GCGC'C Cuts at: 3394 3394 Size: 6313 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3041 3050 928 Size: 971 1142 9 4191 Fragments arranged by size: 4191 1142 971 9 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4504 4538 1903 Size: 34 2567 34 3678 Fragments arranged by size: 3678 2567 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4205 5415 4205 Size: 1210 5103 BclI T'GATC_A Cuts at: 1548 1548 Size: 6313 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4924 136 Size: 107 122 71 631 605 3252 1525 Fragments arranged by size: 3252 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6308 6308 Size: 6313 BlpI GC'TnA_GC Cuts at: 3730 3730 Size: 6313 Bme1580I G_kGCm'C Cuts at: 1790 2385 2447 2742 2813 4374 5530 1790 Size: 595 62 295 71 1561 1156 2573 Fragments arranged by size: 2573 1561 1156 595 295 71 62 BmgBI CAC'GTC Cuts at: 1318 3319 1318 Size: 2001 4312 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4044 5002 442 Size: 416 53 83 399 24 2627 958 1753 Fragments arranged by size: 2627 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6313 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2060 2087 2204 2900 3083 4973 1239 Size: 821 27 117 696 183 1890 2579 Fragments arranged by size: 2579 1890 821 696 183 117 27 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2462 4438 5303 5601 2462 Size: 1976 865 298 3174 Fragments arranged by size: 3174 1976 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2118 3414 4976 882 Size: 617 619 1296 1562 2219 Fragments arranged by size: 2219 1562 1296 619 617 BsaAI yAC'GTr Cuts at: 493 1204 4104 493 Size: 711 2900 2702 Fragments arranged by size: 2900 2702 711 BsaBI GATnn'nnATC Cuts at: 6307 6307 Size: 6313 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3391 4120 4502 5137 Size: 53 83 186 936 1858 729 382 635 Cuts at: 5137 275 Size: 1451 Fragments arranged by size: 1858 1451 936 729 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2029 2421 4746 5144 5418 5565 5976 2029 Size: 392 2325 398 274 147 411 2366 Fragments arranged by size: 2366 2325 411 398 392 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2060 2090 2255 2285 3191 Size: 30 165 30 657 30 165 30 906 Cuts at: 3191 3221 1178 Size: 30 4270 Fragments arranged by size: 4270 906 657 165 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2057 2180 2369 1186 Size: 871 123 189 5130 Fragments arranged by size: 5130 871 189 123 BseYI C'CCAG_C Cuts at: 2084 2322 2396 3377 5516 6012 2084 Size: 238 74 981 2139 496 2385 Fragments arranged by size: 2385 2139 981 496 238 74 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2411 1163 Size: 573 675 5065 Fragments arranged by size: 5065 675 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3763 4524 5552 1540 Size: 275 186 1762 761 1028 2301 Fragments arranged by size: 2301 1762 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2385 2742 2763 2805 4374 4459 5530 2385 Size: 357 21 42 1569 85 1071 3168 Fragments arranged by size: 3168 1569 1071 357 85 42 21 BsmI GAATG_Cn' Cuts at: 3468 3824 3917 3468 Size: 356 93 5864 Fragments arranged by size: 5864 356 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2385 2447 2742 2763 2805 2813 2980 Size: 399 595 62 295 21 42 8 167 Cuts at: 2980 3297 3632 4374 4459 5530 1391 Size: 317 335 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 595 399 335 317 295 167 85 62 42 21 8 BspEI T'CCGG_A Cuts at: 2029 2421 5976 2029 Size: 392 3555 2366 Fragments arranged by size: 3555 2366 392 BspHI T'CATG_A Cuts at: 4202 5932 4202 Size: 1730 4583 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3668 844 Size: 2824 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4200 1394 Size: 429 2377 3507 Fragments arranged by size: 3507 2377 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2710 4808 4982 65 Size: 1183 1462 2098 174 1396 Fragments arranged by size: 2098 1462 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4957 5144 1700 Size: 110 3147 187 2869 Fragments arranged by size: 3147 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2859 96 Size: 2763 3550 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6313 BssSI C'ACGA_G Cuts at: 4373 5385 4373 Size: 1012 5301 BstAPI GCAn_nnn'nTGC Cuts at: 794 2266 2389 794 Size: 1472 123 4718 Fragments arranged by size: 4718 1472 123 BstBI TT'CG_AA Cuts at: 5117 5117 Size: 6313 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6313 BstXI CCAn_nnnn'nTGG Cuts at: 3253 3253 Size: 6313 BstYI r'GATC_y Cuts at: 1972 2400 2488 2679 3643 4010 4395 4412 5864 Size: 428 88 191 964 367 385 17 1452 Cuts at: 5864 5950 5962 6308 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 964 428 385 367 346 191 88 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2543 2543 Size: 6313 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4822 Fragments arranged by size: 4822 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5574 Fragments arranged by size: 5574 683 56 BtsI GCAGTG_nn' Cuts at: 2740 3140 3829 4623 4643 2740 Size: 400 689 794 20 4410 Fragments arranged by size: 4410 794 689 400 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6313 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3134 3169 529 Size: 35 2570 35 3673 Fragments arranged by size: 3673 2570 35 35 DraI TTT'AAA Cuts at: 3617 3617 Size: 6313 DraIII CAC_nnn'GTG Cuts at: 2389 3233 2389 Size: 844 5469 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5320 809 Size: 507 4004 1802 Fragments arranged by size: 4004 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2766 3000 3760 Size: 54 1425 50 275 18 936 234 760 Cuts at: 3760 4649 6278 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 936 889 760 275 234 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3760 1537 Size: 275 1948 4090 Fragments arranged by size: 4090 1948 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3323 3552 4243 1353 Size: 1970 229 691 3423 Fragments arranged by size: 3423 1970 691 229 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3362 4900 5274 5420 387 Size: 1064 138 1773 1538 374 146 1280 Fragments arranged by size: 1773 1538 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2761 2761 Size: 6313 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2060 2087 2093 2192 2204 2900 3083 Size: 393 428 27 6 99 12 696 183 Cuts at: 3083 4376 4973 5760 6028 1239 Size: 1293 597 787 268 1524 Fragments arranged by size: 1524 1293 787 696 597 428 393 268 183 99 27 12 6 EcoNI CCTnn'n_nnAGG Cuts at: 2951 3170 2951 Size: 219 6094 EcoO109I rG'GnC_Cy Cuts at: 2444 3085 3163 3562 2444 Size: 641 78 399 5195 Fragments arranged by size: 5195 641 399 78 EcoRI G'AATT_C Cuts at: 3176 3622 4000 3176 Size: 446 378 5489 Fragments arranged by size: 5489 446 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3067 3067 Size: 6313 FspI TGC'GCA Cuts at: 4819 4819 Size: 6313 HaeII r_GCGC'y Cuts at: 1678 3394 5460 1678 Size: 1716 2066 2531 Fragments arranged by size: 2531 2066 1716 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2042 2060 2074 2092 2255 Size: 32 135 32 470 18 14 18 163 Cuts at: 2255 2287 2967 2999 3341 3347 3373 3379 3527 Size: 32 680 32 342 6 26 6 148 Cuts at: 3527 3559 4954 4986 5028 5060 1373 Size: 32 1395 32 42 32 2626 Fragments arranged by size: 2626 1395 680 470 342 163 148 135 42 32 32 32 32 32 32 32 26 18 18 14 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 3657 3903 669 Size: 988 281 1719 246 3079 Fragments arranged by size: 3079 1719 988 281 246 HindIII A'AGCT_T Cuts at: 748 2105 748 Size: 1357 4956 HpaI GTT'AAC Cuts at: 3903 3903 Size: 6313 KasI G'GCGC_C Cuts at: 3390 3390 Size: 6313 KpnI G_GTAC'C Cuts at: 3638 3638 Size: 6313 MfeI C'AATT_G Cuts at: 3912 3912 Size: 6313 MluI A'CGCG_T Cuts at: 6189 6189 Size: 6313 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2028 2121 2730 3134 5427 5611 1305 Size: 723 93 609 404 2293 184 2007 Fragments arranged by size: 2293 2007 723 609 404 184 93 MscI TGG'CCA Cuts at: 10 64 1489 1832 2768 3002 10 Size: 54 1425 343 936 234 3321 Fragments arranged by size: 3321 1425 936 343 234 54 MslI CAynn'nnrTG Cuts at: 518 1166 2438 2732 2796 2990 3008 3251 4271 Size: 648 1272 294 64 194 18 243 1020 Cuts at: 4271 4630 4789 518 Size: 359 159 2042 Fragments arranged by size: 2042 1272 1020 648 359 294 243 194 159 64 18 MspA1I CmG'CkG Cuts at: 1606 3061 3729 4406 5554 5799 1606 Size: 1455 668 677 1148 245 2120 Fragments arranged by size: 2120 1455 1148 677 668 245 NarI GG'CG_CC Cuts at: 3391 3391 Size: 6313 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4822 Fragments arranged by size: 4822 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6313 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6313 NotI GC'GGCC_GC Cuts at: 3760 3760 Size: 6313 NspI r_CATG'y Cuts at: 1132 1229 3013 3671 5216 1132 Size: 97 1784 658 1545 2229 Fragments arranged by size: 2229 1784 1545 658 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6313 PciI A'CATG_T Cuts at: 3009 5212 3009 Size: 2203 4110 PflMI CCAn_nnn'nTGG Cuts at: 1108 3135 1108 Size: 2027 4286 PfoI T'CCnGG_A Cuts at: 2625 2625 Size: 6313 PmeI GTTT'AAAC Cuts at: 3617 3617 Size: 6313 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4382 4414 5895 5927 4382 Size: 32 1481 32 4768 Fragments arranged by size: 4768 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6313 PsiI TTA'TAA Cuts at: 3883 3883 Size: 6313 PspOMI G'GGCC_C Cuts at: 2443 2443 Size: 6313 PstI C_TGCA'G Cuts at: 830 2262 2785 3665 830 Size: 1432 523 880 3478 Fragments arranged by size: 3478 1432 880 523 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6313 PvuII CAG'CTG Cuts at: 3061 3061 Size: 6313 SacI G_AGCT'C Cuts at: 2763 2763 Size: 6313 SalI G'TCGA_C Cuts at: 1936 3655 1936 Size: 1719 4594 SbfI CC_TGCA'GG Cuts at: 3665 3665 Size: 6313 ScaI AGT'ACT Cuts at: 4561 6273 4561 Size: 1712 4601 SfcI C'TryA_G Cuts at: 826 1045 2258 2781 3661 4796 5477 5668 826 Size: 219 1213 523 880 1135 681 191 1471 Fragments arranged by size: 1471 1213 1135 880 681 523 219 191 SfoI GGC'GCC Cuts at: 3392 3392 Size: 6313 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6313 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6313 SmaI CCC'GGG Cuts at: 1872 2333 3640 1872 Size: 461 1307 4545 Fragments arranged by size: 4545 1307 461 SmlI C'TyrA_G Cuts at: 820 1017 1943 2477 4417 5318 5580 820 Size: 197 926 534 1940 901 262 1553 Fragments arranged by size: 1940 1553 926 901 534 262 197 SnaBI TAC'GTA Cuts at: 493 4104 493 Size: 3611 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6313 SphI G_CATG'C Cuts at: 1132 3671 1132 Size: 2539 3774 SrfI GCCC'GGGC Cuts at: 2333 2333 Size: 6313 SspI AAT'ATT Cuts at: 5 52 4237 5 Size: 47 4185 2081 Fragments arranged by size: 4185 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6313 StyI C'CwwG_G Cuts at: 513 1065 2004 2634 2952 3081 3159 3752 513 Size: 552 939 630 318 129 78 593 3074 Fragments arranged by size: 3074 939 630 593 552 318 129 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2100 2336 3138 4340 4357 4510 4695 Size: 1255 520 236 802 1202 17 153 185 Cuts at: 4695 5140 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1202 802 520 445 236 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2187 2655 2859 Size: 274 80 33 51 1443 210 468 204 Cuts at: 2859 4559 6271 96 Size: 1700 1712 138 Fragments arranged by size: 1712 1700 1443 468 274 210 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2480 2512 6273 6305 2480 Size: 32 3761 32 2488 Fragments arranged by size: 3761 2488 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2839 1316 Size: 1523 4790 XbaI T'CTAG_A Cuts at: 3649 3649 Size: 6313 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3132 3136 3166 3433 3132 Size: 4 30 267 6012 Fragments arranged by size: 6012 267 30 4 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6313 XmaI C'CCGG_G Cuts at: 1870 2331 3638 1870 Size: 461 1307 4545 Fragments arranged by size: 4545 1307 461 XmnI GAAnn'nnTTC Cuts at: 2936 4442 2936 Size: 1506 4807 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4121 276 Size: 53 83 186 936 2587 2468 Fragments arranged by size: 2587 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SwaI