################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiWI BsmI Bsp1407I BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstYI BstZ17I CelII Cfr10I CpoI DraI DrdI Eam1105I Eco47III EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MflI MluI MluNI MroI MscI MseI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AfaI 237 GTAC GT^AC AlwNI 46 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 408 CCTGAGG CC^T[ACGT]AGG AvaI 162 CCCGGG C^[CT]CG[GA]G AviII 344 TGCGCA TGC^GCA BbsI 261 GAAGAC GAAGAC(2/6) BglI 28 GCCATCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 261 GAAGAC GAAGAC(2/6) BsiEI 353 CGGCCG CG[GA][CT]^CG BsmAI 467 GAGAC GTCTC(1/5) BsmBI 467 GAGACG CGTCTC(1/5) BsmFI 458 GGGAC GGGAC(10/14) BsoBI 162 CCCGGG C^[CT]CG[GA]G BspDI 214 ATCGAT AT^CGAT BsrBI 454 GAGCGG CCGCTC(-3/-3) BsrI 479 ACTGG ACTGG(1/-1) BstNI 132 CCTGG CC^[AT]GG BstXI 80 CCACGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 408 CCTGAGG CC^T[ACGT]AGG CfoI 345 GCGC GCG^C ClaI 214 ATCGAT AT^CGAT DdeI 409 CTGAG C^T[ACGT]AG DraIII 391 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 80 CCACGG C^C[GA][CT]GG EagI 353 CGGCCG C^GGCCG EclXI 353 CGGCCG C^GGCCG Eco52I 353 CGGCCG C^GGCCG Eco81I 408 CCTGAGG CC^T[ACGT]AGG EcoNI 420 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRII 132 CCTGG ^CC[AT]GG FspI 344 TGCGCA TGC^GCA HhaI 345 GCGC GCG^C HinP1I 345 GCGC G^CGC MaeII 470 ACGT A^CGT MaeIII 476 GTGAC ^GT[ACGT]AC MslI 243 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 132 CCTGG CC^[AT]GG NspI 347 GCATGT [GA]CATG^[CT] PpuMI 458 GGGACCT [GA]G^G[AT]CC[CT] PstI 67 CTGCAG CTGCA^G PvuII 199 CAGCTG CAG^CTG RsaI 237 GTAC GT^AC SacI 187 GAGCTC GAGCT^C SapI 54 GAAGAGC GCTCTTC(1/4) SfcI 67 CTGCAG C^T[GA][CT]AG SfiI 27 GGCCATCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 162 CCCGGG CCC^GGG SmlI 367 CTCAAG C^T[CT][GA]AG Tsp45I 476 GTGAC ^GT[GC]AC XcmI 266 CCAGCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 162 CCCGGG C^CCGGG XmaI 162 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AspHI 139 GAGCAC G[AT]GC[AT]^C 187 GAGCTC AvaII 405 GGACC G^G[AT]CC 459 GGACC BcnI 162 CCCGG CC^[GC]GG 163 CCGGG BsaJI 80 CCACGG C^C[ACGT][ACGT]GG 162 CCCGGG BsiHKAI 139 GAGCAC G[AT]GC[AT]^C 187 GAGCTC BspMI 461 ACCTGC ACCTGC(4/8) 426 GCAGGT DpnII 213 GATC ^GATC 304 GATC DraII 371 AGGCCCT [GA]G^G[ACGT]CC[CT] 458 GGGACCT EaeI 77 CGGCCA [CT]^GGCC[GA] 353 CGGCCG EarI 54 GAAGAG CTCTTC(1/4) 226 GAAGAG EcoO109I 371 AGGCCCT [GA]G^G[ACGT]CC[CT] 458 GGGACCT HapII 163 CCGG C^CGG 519 CCGG HinfI 535 GAGTC G^A[ACGT]TC 541 GAGTC HpaII 163 CCGG C^CGG 519 CCGG HphI 249 GGTGA GGTGA(8/7) 475 GGTGA Ksp632I 54 GAAGAG CTCTTC(1/4) 226 GAAGAG MboI 213 GATC ^GATC 304 GATC MspA1I 151 CCGCTG C[AC]G^C[GT]G 199 CAGCTG MspI 163 CCGG C^CGG 519 CCGG NciI 162 CCCGG CC^[GC]GG 163 CCGGG NdeII 213 GATC ^GATC 304 GATC PleI 535 GAGTC GAGTC(4/5) 541 GAGTC Sau3AI 213 GATC ^GATC 304 GATC TspRI 142 CACTG CA[GC]TG[ACGT][ACGT]^ 332 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AciI AluI BanII BbvI BmyI BpmI BseRI BsgI BsiYI BslI Bsp1286I BssKI Cac8I Cfr13I FauI Fnu4HI HaeIII ItaI MboII MnlI MwoI NlaIII NlaIV Sau96I ScrFI SfaNI TaqI TseI TthHB8I