(Circular) (Six-base) MAPSORT of: pFN21AE6219.seq Check: 7324 from: 1 to: 5251 pFN21AE6219 5251 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3061 278 Size: 53 83 186 936 1525 2468 Fragments arranged by size: 2468 1525 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2594 1937 Size: 657 4594 Acc65I G'GTAC_C Cuts at: 2572 2572 Size: 5251 AclI AA'CG_TT Cuts at: 1563 3378 3751 1563 Size: 1815 373 3063 Fragments arranged by size: 3063 1815 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2078 2202 2250 2447 3314 4698 4966 1632 Size: 446 124 48 197 867 1384 268 1917 Fragments arranged by size: 1917 1384 867 446 268 197 124 48 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5054 AflIII A'CryG_T Cuts at: 1203 4150 5127 1203 Size: 2947 977 1327 Fragments arranged by size: 2947 1327 977 AgeI A'CCGG_T Cuts at: 4082 4082 Size: 5251 AhdI GACnn_n'nnGTC Cuts at: 3980 3980 Size: 5251 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5251 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2222 1184 Size: 1038 4213 AlwNI CAG_nnn'CTG Cuts at: 2056 2994 4566 2056 Size: 938 1572 2741 Fragments arranged by size: 2741 1572 938 ApaLI G'TGCA_C Cuts at: 3308 4464 3308 Size: 1156 4095 ApoI r'AATT_y Cuts at: 1493 2560 2786 2938 5047 1493 Size: 1067 226 152 2109 1697 Fragments arranged by size: 2109 1697 1067 226 152 AseI AT'TA_AT Cuts at: 160 1032 3805 160 Size: 872 2773 1606 Fragments arranged by size: 2773 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5251 AvaI C'yCGr_G Cuts at: 1870 1943 2167 2576 1870 Size: 73 224 409 4545 Fragments arranged by size: 4545 409 224 73 BamHI G'GATC_C Cuts at: 2581 2948 2581 Size: 367 4884 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2572 4027 618 Size: 325 217 627 785 1455 1842 Fragments arranged by size: 1842 1455 785 627 325 217 BanII G_rGCy'C Cuts at: 1391 2174 2196 2390 2570 1391 Size: 783 22 194 180 4072 Fragments arranged by size: 4072 783 194 180 22 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2273 928 Size: 971 374 3906 Fragments arranged by size: 3906 971 374 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3442 3476 1903 Size: 34 1505 34 3678 Fragments arranged by size: 3678 1505 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3143 4353 3143 Size: 1210 4041 BclI T'GATC_A Cuts at: 1548 1548 Size: 5251 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2039 3862 136 Size: 107 122 71 631 605 367 1823 1525 Fragments arranged by size: 1823 1525 631 605 367 122 107 71 BglII A'GATC_T Cuts at: 5246 5246 Size: 5251 BlpI GC'TnA_GC Cuts at: 2668 2668 Size: 5251 Bme1580I G_kGCm'C Cuts at: 1790 3312 4468 1790 Size: 1522 1156 2573 Fragments arranged by size: 2573 1522 1156 BmgBI CAC'GTC Cuts at: 1318 2476 1318 Size: 1158 4093 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2982 3940 442 Size: 416 53 83 399 24 1565 958 1753 Fragments arranged by size: 1753 1565 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5251 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2114 2303 2506 3911 1239 Size: 875 189 203 1405 2579 Fragments arranged by size: 2579 1405 875 203 189 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2357 3376 4241 4539 2357 Size: 1019 865 298 3069 Fragments arranged by size: 3069 1019 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3914 882 Size: 617 2415 2219 Fragments arranged by size: 2415 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3042 493 Size: 711 1838 2702 Fragments arranged by size: 2702 1838 711 BsaBI GATnn'nnATC Cuts at: 5245 5245 Size: 5251 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3058 3440 4075 275 Size: 53 83 186 936 1525 382 635 1451 Fragments arranged by size: 1525 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3684 4082 4356 4503 4914 3684 Size: 398 274 147 411 4021 Fragments arranged by size: 4021 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5026 Fragments arranged by size: 5026 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2030 2117 2249 2516 1186 Size: 844 87 132 267 3921 Fragments arranged by size: 3921 844 267 132 87 BseYI C'CCAG_C Cuts at: 2383 4454 4950 2383 Size: 2071 496 2684 Fragments arranged by size: 2684 2071 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2192 2207 2282 1163 Size: 573 456 15 75 4132 Fragments arranged by size: 4132 573 456 75 15 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2361 2701 3462 4490 1540 Size: 275 186 360 340 761 1028 2301 Fragments arranged by size: 2301 1028 761 360 340 275 186 BsiHKAI G_wGCw'C Cuts at: 2148 2196 3312 3397 4468 2148 Size: 48 1116 85 1071 2931 Fragments arranged by size: 2931 1116 1071 85 48 BsmI GAATG_Cn' Cuts at: 2762 2855 2762 Size: 93 5158 BsmBI CGTCTCn'nnnn_ Cuts at: 2466 2466 Size: 5251 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2148 2174 2196 2390 2570 3312 3397 Size: 399 358 26 22 194 180 742 85 Cuts at: 3397 4468 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 358 194 180 85 26 22 BspEI T'CCGG_A Cuts at: 4914 4914 Size: 5251 BspHI T'CATG_A Cuts at: 3140 4870 3140 Size: 1730 3521 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2422 2475 2606 844 Size: 1578 53 131 3489 Fragments arranged by size: 3489 1578 131 53 BsrBI CCG'CTC Cuts at: 1394 1823 2461 3138 1394 Size: 429 638 677 3507 Fragments arranged by size: 3507 677 638 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3746 3920 65 Size: 1183 2498 174 1396 Fragments arranged by size: 2498 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3895 4082 1700 Size: 110 2085 187 2869 Fragments arranged by size: 2869 2085 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5251 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5251 BssSI C'ACGA_G Cuts at: 3311 4323 3311 Size: 1012 4239 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5251 BstBI TT'CG_AA Cuts at: 4055 4055 Size: 5251 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5251 BstXI CCAn_nnnn'nTGG Cuts at: 2092 2092 Size: 5251 BstYI r'GATC_y Cuts at: 1972 2581 2948 3333 3350 4802 4888 4900 5246 Size: 609 367 385 17 1452 86 12 346 Cuts at: 5246 1972 Size: 1977 Fragments arranged by size: 1977 1452 609 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2414 2414 Size: 5251 BtgI C'CryG_G Cuts at: 513 1065 2004 2085 513 Size: 552 939 81 3679 Fragments arranged by size: 3679 939 552 81 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4512 Fragments arranged by size: 4512 683 56 BtsI GCAGTG_nn' Cuts at: 2146 2336 2767 3561 3581 2146 Size: 190 431 794 20 3816 Fragments arranged by size: 3816 794 431 190 20 ClaI AT'CG_AT Cuts at: 1554 2220 1554 Size: 666 4585 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5216 DraI TTT'AAA Cuts at: 2555 2555 Size: 5251 DraIII CAC_nnn'GTG Cuts at: 2401 2401 Size: 5251 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4258 809 Size: 507 2942 1802 Fragments arranged by size: 2942 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2082 2358 2698 Size: 54 1425 50 275 18 252 276 340 Cuts at: 2698 3587 5216 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 340 276 275 252 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2358 2698 1537 Size: 275 546 340 4090 Fragments arranged by size: 4090 546 340 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2054 2226 3181 1353 Size: 701 172 955 3423 Fragments arranged by size: 3423 955 701 172 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2156 3838 4212 4358 387 Size: 1064 138 567 1682 374 146 1280 Fragments arranged by size: 1682 1280 1064 567 374 146 138 EcoICRI GAG'CTC Cuts at: 2194 2194 Size: 5251 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2078 2114 2202 2250 2303 2447 2506 Size: 393 446 36 88 48 53 144 59 Cuts at: 2506 3314 3911 4698 4966 1239 Size: 808 597 787 268 1524 Fragments arranged by size: 1524 808 787 597 446 393 268 144 88 59 53 48 36 EcoNI CCTnn'n_nnAGG Cuts at: 2429 2429 Size: 5251 EcoO109I rG'GnC_Cy Cuts at: 2377 2464 2377 Size: 87 5164 EcoRI G'AATT_C Cuts at: 2560 2938 2560 Size: 378 4873 FspI TGC'GCA Cuts at: 2351 3757 2351 Size: 1406 3845 FspAI rTGC'GCAy Cuts at: 2351 2351 Size: 5251 HaeII r_GCGC'y Cuts at: 1678 4398 1678 Size: 2720 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2492 2524 2531 2563 3892 Size: 32 135 32 920 32 7 32 1329 Cuts at: 3892 3924 3966 3998 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1329 920 135 42 32 32 32 32 32 32 7 HincII GTy'rAC Cuts at: 669 1657 1938 2595 2841 669 Size: 988 281 657 246 3079 Fragments arranged by size: 3079 988 657 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5251 HpaI GTT'AAC Cuts at: 2841 2841 Size: 5251 KpnI G_GTAC'C Cuts at: 2576 2576 Size: 5251 MfeI C'AATT_G Cuts at: 2850 2850 Size: 5251 MluI A'CGCG_T Cuts at: 5127 5127 Size: 5251 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4365 4549 1305 Size: 3060 184 2007 Fragments arranged by size: 3060 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3429 Fragments arranged by size: 3429 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2252 3209 3568 3727 518 Size: 648 1086 957 359 159 2042 Fragments arranged by size: 2042 1086 957 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2158 2206 2667 3344 4492 4737 1606 Size: 552 48 461 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 552 461 245 48 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3760 Fragments arranged by size: 3760 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5251 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5251 NotI GC'GGCC_GC Cuts at: 2698 2698 Size: 5251 NspI r_CATG'y Cuts at: 1132 1229 2356 2609 4154 1132 Size: 97 1127 253 1545 2229 Fragments arranged by size: 2229 1545 1127 253 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5251 PciI A'CATG_T Cuts at: 4150 4150 Size: 5251 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5251 PmeI GTTT'AAAC Cuts at: 2555 2555 Size: 5251 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3320 3352 4833 4865 3320 Size: 32 1481 32 3706 Fragments arranged by size: 3706 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2464 2464 Size: 5251 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5251 PsiI TTA'TAA Cuts at: 2821 2821 Size: 5251 PstI C_TGCA'G Cuts at: 830 2076 2603 830 Size: 1246 527 3478 Fragments arranged by size: 3478 1246 527 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5251 PvuII CAG'CTG Cuts at: 2206 2206 Size: 5251 SacI G_AGCT'C Cuts at: 2196 2196 Size: 5251 SalI G'TCGA_C Cuts at: 1936 2593 1936 Size: 657 4594 SapI GCTCTTCn'nnn_ Cuts at: 2054 2054 Size: 5251 SbfI CC_TGCA'GG Cuts at: 2603 2603 Size: 5251 ScaI AGT'ACT Cuts at: 3499 5211 3499 Size: 1712 3539 SfcI C'TryA_G Cuts at: 826 1045 2072 2599 3734 4415 4606 826 Size: 219 1027 527 1135 681 191 1471 Fragments arranged by size: 1471 1135 1027 681 527 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2039 2039 Size: 5251 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5251 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5251 SmaI CCC'GGG Cuts at: 1872 2169 2578 1872 Size: 297 409 4545 Fragments arranged by size: 4545 409 297 SmlI C'TyrA_G Cuts at: 820 1017 1943 2372 3355 4256 4518 820 Size: 197 926 429 983 901 262 1553 Fragments arranged by size: 1553 983 926 901 429 262 197 SnaBI TAC'GTA Cuts at: 493 3042 493 Size: 2549 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5251 SphI G_CATG'C Cuts at: 1132 2609 1132 Size: 1477 3774 SspI AAT'ATT Cuts at: 5 52 3175 5 Size: 47 3123 2081 Fragments arranged by size: 3123 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5251 StyI C'CwwG_G Cuts at: 513 1065 2004 2690 513 Size: 552 939 686 3074 Fragments arranged by size: 3074 939 686 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2468 3278 3295 3448 3633 4078 325 Size: 1255 888 810 17 153 185 445 1498 Fragments arranged by size: 1498 1255 888 810 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3497 5209 96 Size: 274 80 33 51 1443 1520 1712 138 Fragments arranged by size: 1712 1520 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5211 5243 5211 Size: 32 5219 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5251 XbaI T'CTAG_A Cuts at: 2587 2587 Size: 5251 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2278 2278 Size: 5251 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5251 XmaI C'CCGG_G Cuts at: 1870 2167 2576 1870 Size: 297 409 4545 Fragments arranged by size: 4545 409 297 XmnI GAAnn'nnTTC Cuts at: 3380 3380 Size: 5251 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3059 276 Size: 53 83 186 936 1525 2468 Fragments arranged by size: 2468 1525 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfoI SrfI SwaI