Restriction map for
pFN21AE6219
(Linear) (Six-base) MAPSORT of: KIEE6219.seq Check: 9737 from: 1 to: 546
KIEE6219.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 197 245 442 546
Size: 73 124 48 197 104
Fragments arranged by size:
197 124 104 73 48
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 217 546
Size: 217 329
AlwNI CAG_nnn'CTG
Cuts at: 0 51 546
Size: 51 495
AvaI C'yCGr_G
Cuts at: 0 162 546
Size: 162 384
BanII G_rGCy'C
Cuts at: 0 169 191 385 546
Size: 169 22 194 161
Fragments arranged by size:
194 169 161 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 268 546
Size: 268 278
BglI GCCn_nnn'nGGC
Cuts at: 0 34 546
Size: 34 512
BmgBI CAC'GTC
Cuts at: 0 471 546
Size: 471 75
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 298 501 546
Size: 109 189 203 45
Fragments arranged by size:
203 189 109 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 546
Size: 352 194
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 112 244 511 546
Size: 25 87 132 267 35
Fragments arranged by size:
267 132 87 35 25
BseYI C'CCAG_C
Cuts at: 0 378 546
Size: 378 168
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 202 277 546
Size: 187 15 75 269
Fragments arranged by size:
269 187 75 15
BsiEI CG_ry'CG
Cuts at: 0 356 546
Size: 356 190
BsiHKAI G_wGCw'C
Cuts at: 0 143 191 546
Size: 143 48 355
Fragments arranged by size:
355 143 48
BsmBI CGTCTCn'nnnn_
Cuts at: 0 461 546
Size: 461 85
Bsp1286I G_dGCh'C
Cuts at: 0 143 169 191 385 546
Size: 143 26 22 194 161
Fragments arranged by size:
194 161 143 26 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 417 470 546
Size: 417 53 76
Fragments arranged by size:
417 76 53
BsrBI CCG'CTC
Cuts at: 0 456 546
Size: 456 90
BstXI CCAn_nnnn'nTGG
Cuts at: 0 87 546
Size: 87 459
Bsu36I CC'TnA_GG
Cuts at: 0 409 546
Size: 409 137
BtgI C'CryG_G
Cuts at: 0 80 546
Size: 80 466
BtsI GCAGTG_nn'
Cuts at: 0 141 331 546
Size: 141 190 215
Fragments arranged by size:
215 190 141
ClaI AT'CG_AT
Cuts at: 0 215 546
Size: 215 331
DraIII CAC_nnn'GTG
Cuts at: 0 396 546
Size: 396 150
EaeI y'GGCC_r
Cuts at: 0 77 353 546
Size: 77 276 193
Fragments arranged by size:
276 193 77
EagI C'GGCC_G
Cuts at: 0 353 546
Size: 353 193
EarI CTCTTCn'nnn_
Cuts at: 0 49 221 546
Size: 49 172 325
Fragments arranged by size:
325 172 49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 151 546
Size: 151 395
EcoICRI GAG'CTC
Cuts at: 0 189 546
Size: 189 357
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 109 197 245 298 442 501 546
Size: 73 36 88 48 53 144 59 45
Fragments arranged by size:
144 88 73 59 53 48 45 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 424 546
Size: 424 122
EcoO109I rG'GnC_Cy
Cuts at: 0 372 459 546
Size: 372 87 87
Fragments arranged by size:
372 87 87
FspI TGC'GCA
Cuts at: 0 346 546
Size: 346 200
FspAI rTGC'GCAy
Cuts at: 0 346 546
Size: 346 200
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 487 519 526 546
Size: 487 32 7 20
Fragments arranged by size:
487 32 20 7
MslI CAynn'nnrTG
Cuts at: 0 247 546
Size: 247 299
MspA1I CmG'CkG
Cuts at: 0 153 201 546
Size: 153 48 345
Fragments arranged by size:
345 153 48
NspI r_CATG'y
Cuts at: 0 351 546
Size: 351 195
PpuMI rG'GwC_Cy
Cuts at: 0 459 546
Size: 459 87
PstI C_TGCA'G
Cuts at: 0 71 546
Size: 71 475
PvuII CAG'CTG
Cuts at: 0 201 546
Size: 201 345
SacI G_AGCT'C
Cuts at: 0 191 546
Size: 191 355
SapI GCTCTTCn'nnn_
Cuts at: 0 49 546
Size: 49 497
SfcI C'TryA_G
Cuts at: 0 67 546
Size: 67 479
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 34 546
Size: 34 512
SmaI CCC'GGG
Cuts at: 0 164 546
Size: 164 382
SmlI C'TyrA_G
Cuts at: 0 367 546
Size: 367 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 463 546
Size: 463 83
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 273 546
Size: 273 273
XmaI C'CCGG_G
Cuts at: 0 162 546
Size: 162 384
Enzymes that do cut:
AcuI AlfI AlwNI AvaI BanII BbsI BglI BmgBI
BpmI BpuEI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BstXI Bsu36I BtgI BtsI ClaI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FspI FspAI Hin4I MslI MspA1I NspI PpuMI
PstI PvuII SacI SapI SfcI SfiI SmaI SmlI
TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BsiWI BsmI BspEI BspHI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgZI
CspCI DraI DrdI EcoRI EcoRV FalI FseI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TatI TstI Tth111I XbaI XhoI XmnI
ZraI