Restriction map for
pFN21AB6346
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
Acc65I AccI AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AvaI AviII AvrII BalI
BanI BbeI BbsI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaI BsgI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI Eam1105I EarI Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MluI MluNI MscI
MseI MunI NarI NcoI NdeI
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI TfiI Tru9I TspRI
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AatII 799 GACGTC GACGT^C
AccII 558 CGCG CG^CG
AccIII 109 TCCGGA T^CCGGA
AcsI 595 AAATTT [GA]^AATT[CT]
AfaI 732 GTAC GT^AC
AlwNI 25 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 655 GGGCCC GGGCC^C
ApoI 595 AAATTT [GA]^AATT[CT]
Asp700I 381 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 796 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 178 GGATCC G^GATCC
BbrPI 376 CACGTG CAC^GTG
BbvCI 141 CCTCAGC CCTCAGC(-5/-2)
BcgI 756 CGAGGACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 313 CTAG C^TAG
BsaAI 376 CACGTG [CT]AC^GT[GA]
BsaWI 109 TCCGGA [AT]^CCGG[AT]
BseAI 109 TCCGGA T^CCGGA
BsiEI 783 CGGCCG CG[GA][CT]^CG
BspEI 109 TCCGGA T^CCGGA
BsrFI 545 GCCGGC [GA]^CCGG[CT]
BssSI 481 CACGAG CACGAG(-5/-1)
BstF5I 632 GGATG GGATG(2/0)
BstUI 558 CGCG CG^CG
BstXI 432 CCAGGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 178 GGATCC [GA]^GATC[CT]
Cfr10I 545 GCCGGC [GA]^CCGG[CT]
DraIII 562 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 738 CCACGG C^C[GA][CT]GG
EagI 783 CGGCCG C^GGCCG
EclXI 783 CGGCCG C^GGCCG
Eco52I 783 CGGCCG C^GGCCG
EcoT14I 5 CCAAGG C^C[AT][AT]GG
FauI 210 GCGGG CCCGC(4/6)
FokI 632 GGATG GGATG(9/13)
HaeII 426 GGCGCT [GA]GCGC^[CT]
HincII 466 GTCAAC GT[CT]^[GA]AC
HindII 466 GTCAAC GT[CT]^[GA]AC
HinfI 316 GAGTC G^A[ACGT]TC
HphI 773 TCACC GGTGA(8/7)
MaeI 313 CTAG C^TAG
MflI 178 GGATCC [GA]^GATC[CT]
MroI 109 TCCGGA T^CCGGA
MslI 627 CACCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 558 CGCG CG^CG
NaeI 545 GCCGGC GCC^GGC
NgoMIV 545 GCCGGC G^CCGGC
PflFI 796 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 316 GAGTC GAGTC(4/5)
PmaCI 376 CACGTG CAC^GTG
PmlI 376 CACGTG CAC^GTG
RsaI 732 GTAC GT^AC
SexAI 563 ACCTGGT A^CC[AT]GGT
SfaNI 27 GCATC GCATC(5/9)
SmlI 270 CTTGAG C^T[CT][GA]AG
StuI 518 AGGCCT AGG^CCT
StyI 5 CCAAGG C^C[AT][AT]GG
Tsp509I 596 AATT ^AATT
Tth111I 796 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 178 GGATCC [GA]^GATC[CT]
XmnI 381 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 529 GACGCC G[GA]^CG[CT]C
799 GACGTC
AlwI 179 GATCC GGATC(4/5)
178 GGATC
BanII 655 GGGCCC G[GA]GC[CT]^C
742 GGGCTC
BcnI 462 CCCGG CC^[GC]GG
512 CCGGG
BsaHI 529 GACGCC G[GA]^CG[CT]C
799 GACGTC
BsmFI 676 GGGAC GGGAC(10/14)
318 GTCCC
BspMI 398 ACCTGC ACCTGC(4/8)
454 ACCTGC
BsrDI 287 GCAATG GCAATG(2/0)
437 GCAATG
EaeI 735 CGGCCA [CT]^GGCC[GA]
783 CGGCCG
Hin1I 529 GACGCC G[GA]^CG[CT]C
799 GACGTC
MaeIII 47 GTGAC ^GT[ACGT]AC
574 GTCAC
MboII 296 GAAGA GAAGA(8/7)
352 GAAGA
NciI 462 CCCGG CC^[GC]GG
512 CCGGG
PpuMI 341 AGGACCT [GA]G^G[AT]CC[CT]
451 AGGACCT
PstI 94 CTGCAG CTGCA^G
196 CTGCAG
SfcI 94 CTGCAG C^T[GA][CT]AG
196 CTGCAG
Tsp45I 47 GTGAC ^GT[GC]AC
574 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI AspHI AvaII BbvI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmBI Bsp1286I
BsrI BssKI BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII Fnu4HI HaeIII HapII HgaI
HhaI HinP1I HpaII ItaI MaeII
MboI MnlI MspA1I MspI MvaI
MwoI NdeII NlaIV PvuII Sau3AI
Sau96I ScrFI TseI