(Circular) (Six-base) MAPSORT of: pFN21AB6346.seq Check: 1951 from: 1 to: 5578 pFN21AB6346 5578 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2808 3388 278 Size: 53 83 186 936 1272 580 2468 Fragments arranged by size: 2468 1272 936 580 186 83 53 AccI GT'mk_AC Cuts at: 1937 2921 1937 Size: 984 4594 Acc65I G'GTAC_C Cuts at: 2899 2899 Size: 5578 AclI AA'CG_TT Cuts at: 1563 3705 4078 1563 Size: 2142 373 3063 Fragments arranged by size: 3063 2142 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2012 3641 5025 5293 1632 Size: 380 1629 1384 268 1917 Fragments arranged by size: 1917 1629 1384 380 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5381 AflIII A'CryG_T Cuts at: 1203 4477 5454 1203 Size: 3274 977 1327 Fragments arranged by size: 3274 1327 977 AgeI A'CCGG_T Cuts at: 4409 4409 Size: 5578 AhdI GACnn_n'nnGTC Cuts at: 4307 4307 Size: 5578 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5578 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5578 AlwNI CAG_nnn'CTG Cuts at: 2035 3321 4893 2035 Size: 1286 1572 2720 Fragments arranged by size: 2720 1572 1286 ApaI G_GGCC'C Cuts at: 2664 2664 Size: 5578 ApaLI G'TGCA_C Cuts at: 3635 4791 3635 Size: 1156 4422 ApoI r'AATT_y Cuts at: 1493 2600 2887 3113 3265 5374 1493 Size: 1107 287 226 152 2109 1697 Fragments arranged by size: 2109 1697 1107 287 226 152 AseI AT'TA_AT Cuts at: 160 1032 4132 160 Size: 872 3100 1606 Fragments arranged by size: 3100 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5578 AvaI C'yCGr_G Cuts at: 1870 1943 2903 1870 Size: 73 960 4545 Fragments arranged by size: 4545 960 73 BamHI G'GATC_C Cuts at: 2183 2908 3275 2183 Size: 725 367 4486 Fragments arranged by size: 4486 725 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2899 4354 618 Size: 325 217 627 1112 1455 1842 Fragments arranged by size: 1842 1455 1112 627 325 217 BanII G_rGCy'C Cuts at: 1391 2664 2751 2897 1391 Size: 1273 87 146 4072 Fragments arranged by size: 4072 1273 146 87 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4607 BbvCI CC'TCA_GC Cuts at: 2147 2147 Size: 5578 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2750 2784 3769 3803 1903 Size: 34 813 34 985 34 3678 Fragments arranged by size: 3678 985 813 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3470 4680 3470 Size: 1210 4368 BclI T'GATC_A Cuts at: 1548 1548 Size: 5578 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4189 136 Size: 107 122 71 631 605 2517 1525 Fragments arranged by size: 2517 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5573 5573 Size: 5578 BlpI GC'TnA_GC Cuts at: 2995 2995 Size: 5578 Bme1580I G_kGCm'C Cuts at: 1790 2664 2773 3639 4795 1790 Size: 874 109 866 1156 2573 Fragments arranged by size: 2573 1156 874 866 109 BmgBI CAC'GTC Cuts at: 1318 2583 1318 Size: 1265 4313 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2485 3309 4267 Size: 416 53 83 399 24 1068 824 958 Cuts at: 4267 442 Size: 1753 Fragments arranged by size: 1753 1068 958 824 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5578 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2350 2351 2420 2576 2731 4238 1239 Size: 1111 1 69 156 155 1507 2579 Fragments arranged by size: 2579 1507 1111 156 155 69 1 Bpu10I CC'TnA_GC Cuts at: 2147 2414 2147 Size: 267 5311 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2296 3703 4568 4866 2296 Size: 1407 865 298 3008 Fragments arranged by size: 3008 1407 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4241 882 Size: 617 2742 2219 Fragments arranged by size: 2742 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2383 3369 493 Size: 711 1179 986 2702 Fragments arranged by size: 2702 1179 986 711 BsaBI GATnn'nnATC Cuts at: 5572 5572 Size: 5578 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2535 2805 3385 3767 Size: 53 83 186 936 1002 270 580 382 Cuts at: 3767 4402 275 Size: 635 1451 Fragments arranged by size: 1451 1002 936 635 580 382 270 186 83 53 BsaWI w'CCGG_w Cuts at: 2114 4011 4409 4683 4830 5241 2114 Size: 1897 398 274 147 411 2451 Fragments arranged by size: 2451 1897 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2109 2139 2280 2310 1178 Size: 30 165 30 706 30 141 30 4446 Fragments arranged by size: 4446 706 165 141 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2060 2073 2117 2222 2606 2769 1186 Size: 874 13 44 105 384 163 3995 Fragments arranged by size: 3995 874 384 163 105 44 13 BseYI C'CCAG_C Cuts at: 2088 2196 2325 2826 4781 5277 2088 Size: 108 129 501 1955 496 2389 Fragments arranged by size: 2389 1955 501 496 129 108 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5005 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2791 3028 3789 4817 1540 Size: 275 186 790 237 761 1028 2301 Fragments arranged by size: 2301 1028 790 761 275 237 186 BsiHKAI G_wGCw'C Cuts at: 2255 2282 2487 3639 3724 4795 2255 Size: 27 205 1152 85 1071 3038 Fragments arranged by size: 3038 1152 1071 205 85 27 BsmI GAATG_Cn' Cuts at: 3089 3182 3089 Size: 93 5485 BsmBI CGTCTCn'nnnn_ Cuts at: 2111 2222 2687 2111 Size: 111 465 5002 Fragments arranged by size: 5002 465 111 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2255 2282 2487 2664 2751 2773 2897 Size: 399 465 27 205 177 87 22 124 Cuts at: 2897 3639 3724 4795 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 465 399 205 177 124 87 85 27 22 BspEI T'CCGG_A Cuts at: 2114 5241 2114 Size: 3127 2451 BspHI T'CATG_A Cuts at: 3467 5197 3467 Size: 1730 3848 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2412 2468 2933 844 Size: 1568 56 465 3489 Fragments arranged by size: 3489 1568 465 56 BsrBI CCG'CTC Cuts at: 1394 1823 3465 1394 Size: 429 1642 3507 Fragments arranged by size: 3507 1642 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2299 2449 4073 4247 65 Size: 1183 1051 150 1624 174 1396 Fragments arranged by size: 1624 1396 1183 1051 174 150 BsrFI r'CCGG_y Cuts at: 1700 1810 2550 4222 4409 1700 Size: 110 740 1672 187 2869 Fragments arranged by size: 2869 1672 740 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5578 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5578 BssSI C'ACGA_G Cuts at: 2486 3638 4650 2486 Size: 1152 1012 3414 Fragments arranged by size: 3414 1152 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5578 BstBI TT'CG_AA Cuts at: 4382 4382 Size: 5578 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5578 BstXI CCAn_nnnn'nTGG Cuts at: 2444 2444 Size: 5578 BstYI r'GATC_y Cuts at: 1972 2183 2908 3275 3660 3677 5129 5215 5227 Size: 211 725 367 385 17 1452 86 12 Cuts at: 5227 5573 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 725 385 367 346 211 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2743 513 Size: 552 1678 3348 Fragments arranged by size: 3348 1678 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4839 Fragments arranged by size: 4839 683 56 BtsI GCAGTG_nn' Cuts at: 3094 3888 3908 3094 Size: 794 20 4764 Fragments arranged by size: 4764 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5578 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5543 DraI TTT'AAA Cuts at: 2882 2882 Size: 5578 DraIII CAC_nnn'GTG Cuts at: 2572 2572 Size: 5578 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4585 809 Size: 507 3269 1802 Fragments arranged by size: 3269 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2740 2788 3025 Size: 54 1425 50 275 18 910 48 237 Cuts at: 3025 3914 5543 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 910 889 275 237 54 50 48 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2788 3025 1537 Size: 275 976 237 4090 Fragments arranged by size: 4090 976 275 237 EarI CTCTTCn'nnn_ Cuts at: 1353 3508 1353 Size: 2155 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4165 4539 4685 387 Size: 1064 138 2576 374 146 1280 Fragments arranged by size: 2576 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2012 2350 2351 2420 2576 2731 3641 Size: 393 380 338 1 69 156 155 910 Cuts at: 3641 4238 5025 5293 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 910 787 597 393 380 338 268 156 155 69 1 EcoO109I rG'GnC_Cy Cuts at: 2218 2347 2457 2621 2661 2817 2218 Size: 129 110 164 40 156 4979 Fragments arranged by size: 4979 164 156 129 110 40 EcoRI G'AATT_C Cuts at: 2887 3265 2887 Size: 378 5200 FspI TGC'GCA Cuts at: 4084 4084 Size: 5578 HaeII r_GCGC'y Cuts at: 1678 2435 4725 1678 Size: 757 2290 2531 Fragments arranged by size: 2531 2290 757 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2109 2141 2344 2376 4219 Size: 32 135 32 537 32 203 32 1843 Cuts at: 4219 4251 4293 4325 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1843 537 203 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2473 2922 3168 669 Size: 988 281 535 449 246 3079 Fragments arranged by size: 3079 988 535 449 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5578 HpaI GTT'AAC Cuts at: 3168 3168 Size: 5578 KpnI G_GTAC'C Cuts at: 2903 2903 Size: 5578 MfeI C'AATT_G Cuts at: 3177 3177 Size: 5578 MluI A'CGCG_T Cuts at: 5454 5454 Size: 5578 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4692 4876 1305 Size: 3387 184 2007 Fragments arranged by size: 3387 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3756 Fragments arranged by size: 3756 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2636 3536 3895 4054 518 Size: 648 1470 900 359 159 2042 Fragments arranged by size: 2042 1470 900 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2200 2329 2496 2793 2826 2994 3671 4819 Size: 594 129 167 297 33 168 677 1148 Cuts at: 4819 5064 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 594 297 245 168 167 129 33 NaeI GCC'GGC Cuts at: 2552 2552 Size: 5578 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5026 NdeI CA'TA_TG Cuts at: 387 387 Size: 5578 NgoMIV G'CCGG_C Cuts at: 2550 2550 Size: 5578 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5578 NotI GC'GGCC_GC Cuts at: 3025 3025 Size: 5578 NspI r_CATG'y Cuts at: 1132 1229 2936 4481 1132 Size: 97 1707 1545 2229 Fragments arranged by size: 2229 1707 1545 97 PasI CC'CwG_GG Cuts at: 1348 2140 2284 1348 Size: 792 144 4642 Fragments arranged by size: 4642 792 144 PciI A'CATG_T Cuts at: 4477 4477 Size: 5578 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5578 PfoI T'CCnGG_A Cuts at: 2516 2516 Size: 5578 PmeI GTTT'AAAC Cuts at: 2882 2882 Size: 5578 PmlI CAC'GTG Cuts at: 2383 2383 Size: 5578 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3647 3679 5160 5192 3647 Size: 32 1481 32 4033 Fragments arranged by size: 4033 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2347 2457 2347 Size: 110 5468 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5578 PsiI TTA'TAA Cuts at: 3148 3148 Size: 5578 PspOMI G'GGCC_C Cuts at: 2660 2660 Size: 5578 PstI C_TGCA'G Cuts at: 830 2103 2205 2930 830 Size: 1273 102 725 3478 Fragments arranged by size: 3478 1273 725 102 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5578 PvuII CAG'CTG Cuts at: 2200 2329 2496 2200 Size: 129 167 5282 Fragments arranged by size: 5282 167 129 SalI G'TCGA_C Cuts at: 1936 2920 1936 Size: 984 4594 SbfI CC_TGCA'GG Cuts at: 2930 2930 Size: 5578 ScaI AGT'ACT Cuts at: 3826 5538 3826 Size: 1712 3866 SexAI A'CCwGG_T Cuts at: 2568 2568 Size: 5578 SfcI C'TryA_G Cuts at: 826 1045 2099 2201 2926 4061 4742 4933 826 Size: 219 1054 102 725 1135 681 191 1471 Fragments arranged by size: 1471 1135 1054 725 681 219 191 102 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5578 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5578 SmaI CCC'GGG Cuts at: 1872 2905 1872 Size: 1033 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2275 3682 4583 4845 820 Size: 197 926 332 1407 901 262 1553 Fragments arranged by size: 1553 1407 926 901 332 262 197 SnaBI TAC'GTA Cuts at: 493 3369 493 Size: 2876 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5578 SphI G_CATG'C Cuts at: 1132 2936 1132 Size: 1804 3774 SspI AAT'ATT Cuts at: 5 52 3502 5 Size: 47 3450 2081 Fragments arranged by size: 3450 2081 47 StuI AGG'CCT Cuts at: 1517 2525 1517 Size: 1008 4570 StyI C'CwwG_G Cuts at: 513 1065 2010 3017 513 Size: 552 945 1007 3074 Fragments arranged by size: 3074 1007 945 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2297 2467 2662 3605 3622 3775 3960 Size: 1255 717 170 195 943 17 153 185 Cuts at: 3960 4405 325 Size: 445 1498 Fragments arranged by size: 1498 1255 943 717 445 195 185 170 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3824 5536 96 Size: 274 80 33 51 1443 1847 1712 138 Fragments arranged by size: 1847 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2280 2312 5538 5570 2280 Size: 32 3226 32 2288 Fragments arranged by size: 3226 2288 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2804 1316 Size: 1488 4090 XbaI T'CTAG_A Cuts at: 2914 2914 Size: 5578 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5578 XmaI C'CCGG_G Cuts at: 1870 2903 1870 Size: 1033 4545 XmnI GAAnn'nnTTC Cuts at: 2390 3707 2390 Size: 1317 4261 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2806 3386 276 Size: 53 83 186 936 1272 580 2468 Fragments arranged by size: 2468 1272 936 580 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI