################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BbeI BbrPI BbsI BbvCI BciVI BfrI BglI BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiWI BsmBI BspDI BspEI BspMI BsrBI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FseI FspI HaeII HgaI Hin1I HindIII HinfI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I StuI SwaI TfiI Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccII 46 CGCG CG^CG AfaI 717 GTAC GT^AC AflIII 563 ACATGT A^C[GA][CT]GT AocI 579 CCTGAGG CC^T[ACGT]AGG AspHI 658 GAGCTC G[AT]GC[AT]^C AspI 1048 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 468 GGACC G^G[AT]CC BamHI 420 GGATCC G^GATCC BanI 447 GGTGCC G^G[CT][GA]CC BanII 658 GAGCTC G[GA]GC[CT]^C BcgI 704 GCAACACCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 671 TGATCA T^GATCA BfaI 115 CTAG C^TAG BglII 1241 AGATCT A^GATCT BmyI 658 GAGCTC G[AGT]GC[ACT]^C BsaI 459 GAGACC GGTCTC(1/5) BsiEI 62 CGACCG CG[GA][CT]^CG BsiHKAI 658 GAGCTC G[AT]GC[AT]^C BsmI 303 GAATGC GAATGC(1/-1) Bsp1286I 658 GAGCTC G[AGT]GC[ACT]^C Bsp1407I 716 TGTACA T^GTACA BspLU11I 563 ACATGT A^CATGT BsrDI 700 CATTGC GCAATG(2/0) BsrFI 410 GCCGGC [GA]^CCGG[CT] BsrGI 716 TGTACA T^GTACA BstUI 46 CGCG CG^CG Bsu36I 579 CCTGAGG CC^T[ACGT]AGG CfoI 45 GCGC GCG^C Cfr10I 410 GCCGGC [GA]^CCGG[CT] DsaI 464 CCATGG C^C[GA][CT]GG Eco81I 579 CCTGAGG CC^T[ACGT]AGG EcoRI 91 GAATTC G^AATTC EcoT14I 464 CCATGG C^C[AT][AT]GG FbaI 671 TGATCA T^GATCA HhaI 45 GCGC GCG^C HinP1I 45 GCGC G^CGC HpaI 1324 GTTAAC GTT^AAC MaeI 115 CTAG C^TAG MspA1I 569 CCGCTG C[AC]G^C[GT]G MvnI 46 CGCG CG^CG NaeI 410 GCCGGC GCC^GGC NcoI 464 CCATGG C^CATGG NgoMIV 410 GCCGGC G^CCGGC PflFI 1048 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC RsaI 717 GTAC GT^AC SacI 658 GAGCTC GAGCT^C SalI 1216 GTCGAC G^TCGAC SapI 796 GAAGAGC GCTCTTC(1/4) SexAI 956 ACCAGGT A^CC[AT]GGT SmaI 1151 CCCGGG CCC^GGG SmlI 1123 CTTGAG C^T[CT][GA]AG SspBI 716 TGTACA T^GTACA StyI 464 CCATGG C^C[AT][AT]GG Tth111I 1048 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 464 CCATGGACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1151 CCCGGG C^CCGGG XmaI 1151 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AccI 324 GTCTAC GT^[AC][GT]AC 1216 GTCGAC AvaI 232 CCCGAG C^[CT]CG[GA]G 1151 CCCGGG BsmAI 459 GAGAC GTCTC(1/5) 949 GAGAC BsoBI 232 CCCGAG C^[CT]CG[GA]G 1151 CCCGGG Cfr13I 408 GGGCC G^G[ACGT]CC 468 GGACC DdeI 457 CTGAG C^T[ACGT]AG 580 CTGAG HphI 193 GGTGA GGTGA(8/7) 669 GGTGA NspI 563 ACATGT [GA]CATG^[CT] 752 ACATGC Sau96I 408 GGGCC G^G[ACGT]CC 468 GGACC SfaNI 760 GCATC GCATC(5/9) 1260 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI BbvI BcnI BsaJI BseRI BsiYI BslI BsmFI BspHI BssKI BstF5I BstNI BstYI Cac8I DpnII EarI EcoRII FauI Fnu4HI FokI HaeIII HapII HincII HindII HpaII ItaI Ksp632I MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RcaI Sau3AI ScrFI SspI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII