(Circular) (Six-base) MAPSORT of: pFN21AB0461.seq Check: 882 from: 1 to: 6034 pFN21AB0461 6034 bp With 182 enzymes: SgfI * December 8, 2014 13:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3844 278 Size: 53 83 186 936 2308 2468 Fragments arranged by size: 2468 2308 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2330 3222 3377 1937 Size: 393 892 155 4594 Fragments arranged by size: 4594 892 393 155 Acc65I G'GTAC_C Cuts at: 3355 3355 Size: 6034 AclI AA'CG_TT Cuts at: 1563 3333 4161 4534 1563 Size: 1770 828 373 3063 Fragments arranged by size: 3063 1770 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2273 3073 3255 4097 5481 5749 1632 Size: 641 800 182 842 1384 268 1917 Fragments arranged by size: 1917 1384 842 800 641 268 182 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5837 AflIII A'CryG_T Cuts at: 1203 2568 4933 5910 1203 Size: 1365 2365 977 1327 Fragments arranged by size: 2365 1365 1327 977 AgeI A'CCGG_T Cuts at: 4865 4865 Size: 6034 AhdI GACnn_n'nnGTC Cuts at: 4763 4763 Size: 6034 AleI CACnn'nnGTG Cuts at: 1166 2672 2717 1166 Size: 1506 45 4483 Fragments arranged by size: 4483 1506 45 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6034 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2852 2884 2852 Size: 32 6002 AlwNI CAG_nnn'CTG Cuts at: 3777 5349 3777 Size: 1572 4462 ApaLI G'TGCA_C Cuts at: 4091 5247 4091 Size: 1156 4878 ApoI r'AATT_y Cuts at: 1493 2096 2792 3026 3343 3569 3721 5830 1493 Size: 603 696 234 317 226 152 2109 1697 Fragments arranged by size: 2109 1697 696 603 317 234 226 152 AseI AT'TA_AT Cuts at: 160 1032 4588 160 Size: 872 3556 1606 Fragments arranged by size: 3556 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6034 AvaI C'yCGr_G Cuts at: 1870 1943 2237 3156 3359 1870 Size: 73 294 919 203 4545 Fragments arranged by size: 4545 919 294 203 73 BamHI G'GATC_C Cuts at: 2425 3364 3731 2425 Size: 939 367 4728 Fragments arranged by size: 4728 939 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2452 3355 4810 618 Size: 325 217 627 665 903 1455 1842 Fragments arranged by size: 1842 1455 903 665 627 325 217 BanII G_rGCy'C Cuts at: 1391 2667 3353 1391 Size: 1276 686 4072 Fragments arranged by size: 4072 1276 686 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5063 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2698 2732 4225 4259 1903 Size: 34 761 34 1493 34 3678 Fragments arranged by size: 3678 1493 761 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3926 5136 3926 Size: 1210 4824 BclI T'GATC_A Cuts at: 1548 2676 1548 Size: 1128 4906 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4645 136 Size: 107 122 71 631 605 2973 1525 Fragments arranged by size: 2973 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3246 6029 3246 Size: 2783 3251 BlpI GC'TnA_GC Cuts at: 3451 3451 Size: 6034 Bme1580I G_kGCm'C Cuts at: 1790 4095 5251 1790 Size: 2305 1156 2573 Fragments arranged by size: 2573 2305 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6034 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3765 4723 442 Size: 416 53 83 399 24 2348 958 1753 Fragments arranged by size: 2348 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6034 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4694 1239 Size: 3455 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3149 4159 5024 5322 3149 Size: 1010 865 298 3861 Fragments arranged by size: 3861 1010 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2458 4697 882 Size: 617 959 2239 2219 Fragments arranged by size: 2239 2219 959 617 BsaAI yAC'GTr Cuts at: 493 1204 3825 493 Size: 711 2621 2702 Fragments arranged by size: 2702 2621 711 BsaBI GATnn'nnATC Cuts at: 6028 6028 Size: 6034 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3841 4223 4858 275 Size: 53 83 186 936 2308 382 635 1451 Fragments arranged by size: 2308 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4467 4865 5139 5286 5697 4467 Size: 398 274 147 411 4804 Fragments arranged by size: 4804 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2519 2549 1178 Size: 30 165 30 1116 30 4663 Fragments arranged by size: 4663 1116 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2423 2675 1186 Size: 838 399 252 4545 Fragments arranged by size: 4545 838 399 252 BseYI C'CCAG_C Cuts at: 2301 5237 5733 2301 Size: 2936 496 2602 Fragments arranged by size: 2936 2602 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5461 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2070 3484 4245 5273 1540 Size: 275 186 69 1414 761 1028 2301 Fragments arranged by size: 2301 1414 1028 761 275 186 69 BsiHKAI G_wGCw'C Cuts at: 2667 4095 4180 5251 2667 Size: 1428 85 1071 3450 Fragments arranged by size: 3450 1428 1071 85 BsmI GAATG_Cn' Cuts at: 2314 3545 3638 2314 Size: 1231 93 4710 Fragments arranged by size: 4710 1231 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2667 3353 4095 4180 5251 1391 Size: 399 877 686 742 85 1071 2174 Fragments arranged by size: 2174 1071 877 742 686 399 85 BspEI T'CCGG_A Cuts at: 5697 5697 Size: 6034 BspHI T'CATG_A Cuts at: 2892 2968 3063 3923 5653 2892 Size: 76 95 860 1730 3273 Fragments arranged by size: 3273 1730 860 95 76 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3389 844 Size: 2545 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3921 1394 Size: 429 2098 3507 Fragments arranged by size: 3507 2098 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2704 4529 4703 65 Size: 1183 1456 1825 174 1396 Fragments arranged by size: 1825 1456 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2415 4678 4865 1700 Size: 110 605 2263 187 2869 Fragments arranged by size: 2869 2263 605 187 110 BsrGI T'GTAC_A Cuts at: 96 2721 96 Size: 2625 3409 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6034 BssSI C'ACGA_G Cuts at: 4094 5106 4094 Size: 1012 5022 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6034 BstBI TT'CG_AA Cuts at: 4838 4838 Size: 6034 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6034 BstYI r'GATC_y Cuts at: 1972 2425 2812 2926 3246 3364 3731 4116 4133 Size: 453 387 114 320 118 367 385 17 Cuts at: 4133 5585 5671 5683 6029 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 453 387 385 367 346 320 118 114 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2585 2585 Size: 6034 BtgI C'CryG_G Cuts at: 513 1065 2004 2469 513 Size: 552 939 465 4078 Fragments arranged by size: 4078 939 552 465 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5295 Fragments arranged by size: 5295 683 56 BtsI GCAGTG_nn' Cuts at: 3294 3550 4344 4364 3294 Size: 256 794 20 4964 Fragments arranged by size: 4964 794 256 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6034 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5999 DraI TTT'AAA Cuts at: 3338 3338 Size: 6034 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5041 809 Size: 507 3725 1802 Fragments arranged by size: 3725 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3481 4370 5999 Size: 54 1425 50 275 18 1651 889 1629 Cuts at: 5999 8 Size: 43 Fragments arranged by size: 1651 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3481 1537 Size: 275 1669 4090 Fragments arranged by size: 4090 1669 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2249 2494 2796 2854 3964 1353 Size: 896 245 302 58 1110 3423 Fragments arranged by size: 3423 1110 896 302 245 58 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4621 4995 5141 387 Size: 1064 138 3032 374 146 1280 Fragments arranged by size: 3032 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2665 2665 Size: 6034 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2273 3073 3255 4097 4694 5481 5749 Size: 393 641 800 182 842 597 787 268 Cuts at: 5749 1239 Size: 1524 Fragments arranged by size: 1524 842 800 787 641 597 393 268 182 EcoRI G'AATT_C Cuts at: 2096 3343 3721 2096 Size: 1247 378 4409 Fragments arranged by size: 4409 1247 378 FspI TGC'GCA Cuts at: 4540 4540 Size: 6034 HaeII r_GCGC'y Cuts at: 1678 5181 1678 Size: 3503 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2069 2101 2164 2196 4675 Size: 32 135 32 497 32 63 32 2479 Cuts at: 4675 4707 4749 4781 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2479 497 135 63 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2548 3223 3331 3378 3624 669 Size: 988 281 610 675 108 47 246 3079 Fragments arranged by size: 3079 988 675 610 281 246 108 47 HindIII A'AGCT_T Cuts at: 748 748 Size: 6034 HpaI GTT'AAC Cuts at: 3331 3624 3331 Size: 293 5741 KpnI G_GTAC'C Cuts at: 3359 3359 Size: 6034 MfeI C'AATT_G Cuts at: 3633 3633 Size: 6034 MluI A'CGCG_T Cuts at: 5910 5910 Size: 6034 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5148 5332 1305 Size: 3843 184 2007 Fragments arranged by size: 3843 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4212 Fragments arranged by size: 4212 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2061 2672 2717 3992 4351 4510 518 Size: 648 895 611 45 1275 359 159 2042 Fragments arranged by size: 2042 1275 895 648 611 359 159 45 MspA1I CmG'CkG Cuts at: 1606 2576 3450 4127 5275 5520 1606 Size: 970 874 677 1148 245 2120 Fragments arranged by size: 2120 1148 970 874 677 245 NaeI GCC'GGC Cuts at: 2417 2417 Size: 6034 NcoI C'CATG_G Cuts at: 513 1065 2004 2469 513 Size: 552 939 465 4078 Fragments arranged by size: 4078 939 552 465 NdeI CA'TA_TG Cuts at: 387 387 Size: 6034 NgoMIV G'CCGG_C Cuts at: 2415 2415 Size: 6034 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6034 NotI GC'GGCC_GC Cuts at: 3481 3481 Size: 6034 NspI r_CATG'y Cuts at: 1132 1229 2572 2761 3392 4937 1132 Size: 97 1343 189 631 1545 2229 Fragments arranged by size: 2229 1545 1343 631 189 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6034 PciI A'CATG_T Cuts at: 2568 4933 2568 Size: 2365 3669 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6034 PfoI T'CCnGG_A Cuts at: 2421 3318 2421 Size: 897 5137 PmeI GTTT'AAAC Cuts at: 3338 3338 Size: 6034 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4103 4135 5616 5648 4103 Size: 32 1481 32 4489 Fragments arranged by size: 4489 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6034 PsiI TTA'TAA Cuts at: 3604 3604 Size: 6034 PstI C_TGCA'G Cuts at: 830 3386 830 Size: 2556 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6034 SacI G_AGCT'C Cuts at: 2667 2667 Size: 6034 SalI G'TCGA_C Cuts at: 1936 3221 3376 1936 Size: 1285 155 4594 Fragments arranged by size: 4594 1285 155 SapI GCTCTTCn'nnn_ Cuts at: 2796 2796 Size: 6034 SbfI CC_TGCA'GG Cuts at: 3386 3386 Size: 6034 ScaI AGT'ACT Cuts at: 4282 5994 4282 Size: 1712 4322 SexAI A'CCwGG_T Cuts at: 2961 2961 Size: 6034 SfcI C'TryA_G Cuts at: 826 1045 3382 4517 5198 5389 826 Size: 219 2337 1135 681 191 1471 Fragments arranged by size: 2337 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6034 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6034 SmaI CCC'GGG Cuts at: 1872 3158 3361 1872 Size: 1286 203 4545 Fragments arranged by size: 4545 1286 203 SmlI C'TyrA_G Cuts at: 820 1017 1943 3128 4138 5039 5301 820 Size: 197 926 1185 1010 901 262 1553 Fragments arranged by size: 1553 1185 1010 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3825 493 Size: 3332 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6034 SphI G_CATG'C Cuts at: 1132 3392 1132 Size: 2260 3774 SspI AAT'ATT Cuts at: 5 52 2154 2632 3280 3958 5 Size: 47 2102 478 648 678 2081 Fragments arranged by size: 2102 2081 678 648 478 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6034 StyI C'CwwG_G Cuts at: 513 1065 2004 2469 3473 513 Size: 552 939 465 1004 3074 Fragments arranged by size: 3074 1004 939 552 465 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2084 4061 4078 4231 4416 4861 325 Size: 1255 504 1977 17 153 185 445 1498 Fragments arranged by size: 1977 1498 1255 504 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2721 4280 5992 Size: 274 80 33 51 1443 744 1559 1712 Cuts at: 5992 96 Size: 138 Fragments arranged by size: 1712 1559 1443 744 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2519 2551 5994 6026 2519 Size: 32 3443 32 2527 Fragments arranged by size: 3443 2527 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3056 1316 Size: 1740 4294 XbaI T'CTAG_A Cuts at: 3370 3370 Size: 6034 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2476 2476 Size: 6034 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6034 XmaI C'CCGG_G Cuts at: 1870 3156 3359 1870 Size: 1286 203 4545 Fragments arranged by size: 4545 1286 203 XmnI GAAnn'nnTTC Cuts at: 4163 4163 Size: 6034 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3842 276 Size: 53 83 186 936 2308 2468 Fragments arranged by size: 2468 2308 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SfiI SfoI SrfI SwaI