Restriction map for
pFN21AE5614
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# NO CUT ENZYMES ( 126 enzymes) #
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AccI AccII AccIII AclI AflII
AgeI AhdI Aor51HI ApaI AscI
AseI AsnI Asp700I AspEI AviII
AvrII BamHI BanII BbeI BbrPI
BbsI BcgI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstUI BstXI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoRI FseI FspI HaeII HgaI
HhaI HinP1I KasI KspI MfeI
MluI MroI MspA1I MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 754 GACGTC GACGT^C
Acc65I 1325 GGTACC G^GTACC
AcyI 754 GACGTC G[GA]^CG[CT]C
AflIII 1319 ACATGT A^C[GA][CT]GT
AlwI 1009 GGATC GGATC(4/5)
AlwNI 1164 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 1325 GGTACC G^GTACC
AspI 1082 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1158 CCCGAG C^[CT]CG[GA]G
BbvCI 1299 CCTCAGC CCTCAGC(-5/-2)
BclI 36 TGATCA T^GATCA
BcnI 1415 CCCGG CC^[GC]GG
BglI 923 GCCTGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 540 GATATCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 754 GACGTC G[GA]^CG[CT]C
BsaI 1087 GGTCTC GGTCTC(1/5)
BsoBI 1158 CCCGAG C^[CT]CG[GA]G
BspLU11I 1319 ACATGT A^CATGT
BstEII 980 GGTTACC G^GT[ACGT]ACC
BstPI 980 GGTTACC G^GT[ACGT]ACC
BstYI 1009 GGATCT [GA]^GATC[CT]
DraIII 574 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 980 GGTTACC G^GT[ACGT]ACC
EcoT14I 1497 CCAAGG C^C[AT][AT]GG
FauI 1482 GCGGG CCCGC(4/6)
FbaI 36 TGATCA T^GATCA
Hin1I 754 GACGTC G[GA]^CG[CT]C
HindIII 999 AAGCTT A^AGCTT
HpaI 262 GTTAAC GTT^AAC
KpnI 1325 GGTACC GGTAC^C
MamI 540 GATATCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1009 GGATCT [GA]^GATC[CT]
MseI 263 TTAA T^TAA
MslI 1521 CACCCTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 1415 CCCGG CC^[GC]GG
PflFI 1082 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 53 GAAGAGC GCTCTTC(1/4)
SmlI 1207 CTCAAG C^T[CT][GA]AG
StuI 1112 AGGCCT AGG^CCT
StyI 1497 CCAAGG C^C[AT][AT]GG
Tru9I 263 TTAA T^TAA
Tth111I 1082 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 458 CCAGCTTCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1009 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 30 enzymes) #
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AciI 838 GCGG CCGC(-3/-1)
1482 GCGG
AcsI 390 GAATTT [GA]^AATT[CT]
1202 AAATTC
Alw44I 580 GTGCAC G^TGCAC
855 GTGCAC
AocI 326 CCTTAGG CC^T[ACGT]AGG
1144 CCTGAGG
ApaLI 580 GTGCAC G^TGCAC
855 GTGCAC
ApoI 390 GAATTT [GA]^AATT[CT]
1202 AAATTC
AspHI 580 GTGCAC G[AT]GC[AT]^C
855 GTGCAC
BalI 623 TGGCCA TGG^CCA
930 TGGCCA
BanI 169 GGTGCC G^G[CT][GA]CC
1325 GGTACC
BciVI 870 GGATAC GTATCC(6/5)
1485 GGATAC
BfaI 728 CTAG C^TAG
1525 CTAG
BsiHKAI 580 GTGCAC G[AT]GC[AT]^C
855 GTGCAC
BsrDI 1425 CATTGC GCAATG(2/0)
160 GCAATG
Bsu36I 326 CCTTAGG CC^T[ACGT]AGG
1144 CCTGAGG
EaeI 623 TGGCCA [CT]^GGCC[GA]
930 TGGCCA
EarI 53 GAAGAG CTCTTC(1/4)
763 GAAGAG
Eco81I 326 CCTTAGG CC^T[ACGT]AGG
1144 CCTGAGG
EcoRV 540 GATATC GAT^ATC
664 GATATC
HapII 316 CCGG C^CGG
1416 CCGG
HpaII 316 CCGG C^CGG
1416 CCGG
Ksp632I 53 GAAGAG CTCTTC(1/4)
763 GAAGAG
MaeI 728 CTAG C^TAG
1525 CTAG
MaeII 260 ACGT A^CGT
755 ACGT
MluNI 623 TGGCCA TGG^CCA
930 TGGCCA
MscI 623 TGGCCA TGG^CCA
930 TGGCCA
MspI 316 CCGG C^CGG
1416 CCGG
PstI 788 CTGCAG CTGCA^G
1045 CTGCAG
SphI 119 GCATGC GCATG^C
453 GCATGC
TaqI 721 TCGA T^CGA
1080 TCGA
TthHB8I 721 TCGA T^CGA
1080 TCGA
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AfaI AluI AvaII BbvI BmyI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
EcoT22I Fnu4HI FokI HaeIII HincII
HindII HinfI HphI ItaI MaeIII
MboI MboII MnlI MvaI MwoI
NdeII NlaIII NlaIV NsiI NspI
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TfiI TseI
Tsp45I Tsp509I TspRI