(Circular) (Six-base) MAPSORT of: pFN21AE5614.seq Check: 1240 from: 1 to: 6298 pFN21AE5614 6298 bp With 182 enzymes: SgfI * December 8, 2014 13:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2763 4108 278 Size: 53 83 186 936 1227 1345 2468 Fragments arranged by size: 2468 1345 1227 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3641 1937 Size: 1704 4594 Acc65I G'GTAC_C Cuts at: 3330 3619 3330 Size: 289 6009 AclI AA'CG_TT Cuts at: 1563 4425 4798 1563 Size: 2862 373 3063 Fragments arranged by size: 3063 2862 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2001 2522 2618 2991 3263 4361 5745 6013 Size: 369 521 96 373 272 1098 1384 268 Cuts at: 6013 1632 Size: 1917 Fragments arranged by size: 1917 1384 1098 521 373 369 272 268 96 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6101 AflIII A'CryG_T Cuts at: 1203 3324 5197 6174 1203 Size: 2121 1873 977 1327 Fragments arranged by size: 2121 1873 1327 977 AgeI A'CCGG_T Cuts at: 5129 5129 Size: 6298 AhdI GACnn_n'nnGTC Cuts at: 5027 5027 Size: 6298 AleI CACnn'nnGTG Cuts at: 1166 3530 1166 Size: 2364 3934 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6298 AlwNI CAG_nnn'CTG Cuts at: 3174 4041 5613 3174 Size: 867 1572 3859 Fragments arranged by size: 3859 1572 867 ApaLI G'TGCA_C Cuts at: 2585 2860 4355 5511 2585 Size: 275 1495 1156 3372 Fragments arranged by size: 3372 1495 1156 275 ApoI r'AATT_y Cuts at: 1493 2395 3207 3607 3833 3985 6094 1493 Size: 902 812 400 226 152 2109 1697 Fragments arranged by size: 2109 1697 902 812 400 226 152 AseI AT'TA_AT Cuts at: 160 1032 4852 160 Size: 872 3820 1606 Fragments arranged by size: 3820 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6298 AvaI C'yCGr_G Cuts at: 1870 1943 3163 3623 1870 Size: 73 1220 460 4545 Fragments arranged by size: 4545 1220 460 73 BamHI G'GATC_C Cuts at: 3628 3995 3628 Size: 367 5931 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2174 3330 3619 5074 618 Size: 325 217 627 387 1156 289 1455 1842 Fragments arranged by size: 1842 1455 1156 627 387 325 289 217 BanII G_rGCy'C Cuts at: 1391 3617 1391 Size: 2226 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5327 BbvCI CC'TCA_GC Cuts at: 3305 3305 Size: 6298 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4489 4523 1903 Size: 34 2552 34 3678 Fragments arranged by size: 3678 2552 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2869 3484 4190 5400 2869 Size: 615 706 1210 3767 Fragments arranged by size: 3767 1210 706 615 BclI T'GATC_A Cuts at: 1548 2041 1548 Size: 493 5805 BfrBI ATG'CAT Cuts at: 2128 2170 2458 2128 Size: 42 288 5968 Fragments arranged by size: 5968 288 42 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2934 4909 136 Size: 107 122 71 631 605 1262 1975 1525 Fragments arranged by size: 1975 1525 1262 631 605 122 107 71 BglII A'GATC_T Cuts at: 6293 6293 Size: 6298 BlpI GC'TnA_GC Cuts at: 3715 3715 Size: 6298 Bme1580I G_kGCm'C Cuts at: 1790 2179 2589 2864 3537 4359 5515 1790 Size: 389 410 275 673 822 1156 2573 Fragments arranged by size: 2573 1156 822 673 410 389 275 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6298 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2426 2849 4029 Size: 416 53 83 399 24 1009 423 1180 Cuts at: 4029 4987 442 Size: 958 1753 Fragments arranged by size: 1753 1180 1009 958 423 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6298 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2175 2277 2866 3099 3532 4958 1239 Size: 936 102 589 233 433 1426 2579 Fragments arranged by size: 2579 1426 936 589 433 233 102 Bpu10I CC'TnA_GC Cuts at: 3131 3305 3131 Size: 174 6124 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3197 4423 5288 5586 3197 Size: 1226 865 298 3909 Fragments arranged by size: 3909 1226 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3098 4961 882 Size: 617 1599 1863 2219 Fragments arranged by size: 2219 1863 1599 617 BsaAI yAC'GTr Cuts at: 493 1204 4089 493 Size: 711 2885 2702 Fragments arranged by size: 2885 2702 711 BsaBI GATnn'nnATC Cuts at: 2549 6292 2549 Size: 3743 2555 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2760 4105 4487 5122 Size: 53 83 186 936 1227 1345 382 635 Cuts at: 5122 275 Size: 1451 Fragments arranged by size: 1451 1345 1227 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4731 5129 5403 5550 5961 4731 Size: 398 274 147 411 5068 Fragments arranged by size: 5068 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2585 2615 2879 2909 3078 Size: 30 165 30 1182 30 264 30 169 Cuts at: 3078 3108 3293 3323 1178 Size: 30 185 30 4153 Fragments arranged by size: 4153 1182 264 185 169 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6298 BseYI C'CCAG_C Cuts at: 5501 5997 5501 Size: 496 5802 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5725 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3748 4509 5537 1540 Size: 275 186 1747 761 1028 2301 Fragments arranged by size: 2301 1747 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2589 2864 4359 4444 5515 2589 Size: 275 1495 85 1071 3372 Fragments arranged by size: 3372 1495 1071 275 85 BsmI GAATG_Cn' Cuts at: 2128 2417 2485 3135 3809 3902 2128 Size: 289 68 650 674 93 4524 Fragments arranged by size: 4524 674 650 289 93 68 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2179 2589 2864 3537 3617 4359 4444 Size: 399 389 410 275 673 80 742 85 Cuts at: 4444 5515 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 673 410 399 389 275 85 80 BspEI T'CCGG_A Cuts at: 5961 5961 Size: 6298 BspHI T'CATG_A Cuts at: 4187 5917 4187 Size: 1730 4568 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3653 844 Size: 2809 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4185 1394 Size: 429 2362 3507 Fragments arranged by size: 3507 2362 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2172 3429 4793 4967 65 Size: 1183 924 1257 1364 174 1396 Fragments arranged by size: 1396 1364 1257 1183 924 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4942 5129 1700 Size: 110 3132 187 2869 Fragments arranged by size: 3132 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6298 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6298 BssSI C'ACGA_G Cuts at: 4358 5370 4358 Size: 1012 5286 BstAPI GCAn_nnn'nTGC Cuts at: 794 3049 3174 3586 794 Size: 2255 125 412 3506 Fragments arranged by size: 3506 2255 412 125 BstBI TT'CG_AA Cuts at: 5102 5102 Size: 6298 BstEII G'GTnAC_C Cuts at: 1184 2985 1184 Size: 1801 4497 BstYI r'GATC_y Cuts at: 1972 3014 3628 3995 4380 4397 5849 5935 5947 Size: 1042 614 367 385 17 1452 86 12 Cuts at: 5947 6293 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1042 614 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2332 3150 2332 Size: 818 5480 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5746 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2473 487 Size: 683 56 1247 4312 Fragments arranged by size: 4312 1247 683 56 BtsI GCAGTG_nn' Cuts at: 2009 2422 3814 4608 4628 2009 Size: 413 1392 794 20 3679 Fragments arranged by size: 3679 1392 794 413 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6298 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6263 DraI TTT'AAA Cuts at: 3602 3602 Size: 6298 DraIII CAC_nnn'GTG Cuts at: 2584 2584 Size: 6298 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5305 809 Size: 507 3989 1802 Fragments arranged by size: 3989 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2628 2935 3745 Size: 54 1425 50 275 18 798 307 810 Cuts at: 3745 4634 6263 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 810 798 307 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3745 1537 Size: 275 1933 4090 Fragments arranged by size: 4090 1933 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2053 2763 4228 1353 Size: 700 710 1465 3423 Fragments arranged by size: 3423 1465 710 700 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4885 5259 5405 387 Size: 1064 138 3296 374 146 1280 Fragments arranged by size: 3296 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2001 2175 2277 2522 2618 2866 2991 Size: 393 369 174 102 245 96 248 125 Cuts at: 2991 3099 3263 3532 4361 4958 5745 6013 1239 Size: 108 164 269 829 597 787 268 1524 Fragments arranged by size: 1524 829 787 597 393 369 269 268 248 245 174 164 125 108 102 96 EcoO109I rG'GnC_Cy Cuts at: 2328 3217 3507 2328 Size: 889 290 5119 Fragments arranged by size: 5119 889 290 EcoRI G'AATT_C Cuts at: 3607 3985 3607 Size: 378 5920 EcoRV GAT'ATC Cuts at: 2547 2671 2547 Size: 124 6174 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3022 3022 Size: 6298 FspI TGC'GCA Cuts at: 4804 4804 Size: 6298 HaeII r_GCGC'y Cuts at: 1678 5445 1678 Size: 3767 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2585 2617 3078 3110 3293 Size: 32 135 32 1013 32 461 32 183 Cuts at: 3293 3325 4939 4971 5013 5045 1373 Size: 32 1614 32 42 32 2626 Fragments arranged by size: 2626 1614 1013 461 183 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2269 2560 3364 3642 3888 669 Size: 988 281 331 291 804 278 246 3079 Fragments arranged by size: 3079 988 804 331 291 281 278 246 HindIII A'AGCT_T Cuts at: 748 3004 748 Size: 2256 4042 HpaI GTT'AAC Cuts at: 2269 3888 2269 Size: 1619 4679 KpnI G_GTAC'C Cuts at: 3334 3623 3334 Size: 289 6009 MfeI C'AATT_G Cuts at: 3897 3897 Size: 6298 MluI A'CGCG_T Cuts at: 6174 6174 Size: 6298 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5412 5596 1305 Size: 4107 184 2007 Fragments arranged by size: 4107 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2630 2937 10 Size: 54 1425 343 798 307 3371 Fragments arranged by size: 3371 1425 798 343 307 54 MslI CAynn'nnrTG Cuts at: 518 1166 3530 4256 4615 4774 518 Size: 648 2364 726 359 159 2042 Fragments arranged by size: 2364 2042 726 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3714 4391 5539 5784 1606 Size: 2108 677 1148 245 2120 Fragments arranged by size: 2120 2108 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5746 NdeI CA'TA_TG Cuts at: 387 387 Size: 6298 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6298 NotI GC'GGCC_GC Cuts at: 3745 3745 Size: 6298 NsiI A_TGCA'T Cuts at: 2130 2172 2460 2130 Size: 42 288 5968 Fragments arranged by size: 5968 288 42 NspI r_CATG'y Cuts at: 1132 1229 2128 2320 2458 2462 3328 3656 5201 Size: 97 899 192 138 4 866 328 1545 Cuts at: 5201 1132 Size: 2229 Fragments arranged by size: 2229 1545 899 866 328 192 138 97 4 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6298 PciI A'CATG_T Cuts at: 3324 5197 3324 Size: 1873 4425 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6298 PfoI T'CCnGG_A Cuts at: 2901 2901 Size: 6298 PmeI GTTT'AAAC Cuts at: 3602 3602 Size: 6298 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4367 4399 5880 5912 4367 Size: 32 1481 32 4753 Fragments arranged by size: 4753 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2328 3217 3507 2328 Size: 889 290 5119 Fragments arranged by size: 5119 889 290 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6298 PsiI TTA'TAA Cuts at: 3868 3868 Size: 6298 PstI C_TGCA'G Cuts at: 830 2797 3054 3650 830 Size: 1967 257 596 3478 Fragments arranged by size: 3478 1967 596 257 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6298 SalI G'TCGA_C Cuts at: 1936 3640 1936 Size: 1704 4594 SanDI GG'GwC_CC Cuts at: 3507 3507 Size: 6298 SapI GCTCTTCn'nnn_ Cuts at: 2053 2053 Size: 6298 SbfI CC_TGCA'GG Cuts at: 3650 3650 Size: 6298 ScaI AGT'ACT Cuts at: 4546 6258 4546 Size: 1712 4586 SfcI C'TryA_G Cuts at: 826 1045 2781 2793 3050 3646 4781 5462 5653 Size: 219 1736 12 257 596 1135 681 191 Cuts at: 5653 826 Size: 1471 Fragments arranged by size: 1736 1471 1135 681 596 257 219 191 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6298 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6298 SmaI CCC'GGG Cuts at: 1872 3625 1872 Size: 1753 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3212 4402 5303 5565 820 Size: 197 926 1269 1190 901 262 1553 Fragments arranged by size: 1553 1269 1190 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4089 493 Size: 3596 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6298 SphI G_CATG'C Cuts at: 1132 2128 2462 3656 1132 Size: 996 334 1194 3774 Fragments arranged by size: 3774 1194 996 334 SspI AAT'ATT Cuts at: 5 52 4222 5 Size: 47 4170 2081 Fragments arranged by size: 4170 2081 47 StuI AGG'CCT Cuts at: 1517 3119 1517 Size: 1602 4696 StyI C'CwwG_G Cuts at: 513 1065 3502 3737 513 Size: 552 2437 235 3074 Fragments arranged by size: 3074 2437 552 235 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2962 4325 4342 4495 4680 5125 325 Size: 1255 1382 1363 17 153 185 445 1498 Fragments arranged by size: 1498 1382 1363 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4544 6256 96 Size: 274 80 33 51 1443 2567 1712 138 Fragments arranged by size: 2567 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3499 3531 6258 6290 3499 Size: 32 2727 32 3507 Fragments arranged by size: 3507 2727 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3090 1316 Size: 1774 4524 XbaI T'CTAG_A Cuts at: 3634 3634 Size: 6298 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2470 2470 Size: 6298 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6298 XmaI C'CCGG_G Cuts at: 1870 3623 1870 Size: 1753 4545 XmnI GAAnn'nnTTC Cuts at: 4427 4427 Size: 6298 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2761 4106 276 Size: 53 83 186 936 1227 1345 2468 Fragments arranged by size: 2468 1345 1227 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI