(Linear) (Six-base) MAPSORT of: KIEE5614.seq Check: 834 from: 1 to: 1593 KIEE5614.orf With 182 enzymes: SgfI * December 5, 2014 13:03 .. AatII G_ACGT'C Cuts at: 0 758 1593 Size: 758 835 Acc65I G'GTAC_C Cuts at: 0 1325 1593 Size: 1325 268 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 517 613 986 1258 1593 Size: 517 96 373 272 335 Fragments arranged by size: 517 373 335 272 96 AflIII A'CryG_T Cuts at: 0 1319 1593 Size: 1319 274 AleI CACnn'nnGTG Cuts at: 0 1525 1593 Size: 1525 68 AlwNI CAG_nnn'CTG Cuts at: 0 1169 1593 Size: 1169 424 ApaLI G'TGCA_C Cuts at: 0 580 855 1593 Size: 580 275 738 Fragments arranged by size: 738 580 275 ApoI r'AATT_y Cuts at: 0 390 1202 1593 Size: 390 812 391 Fragments arranged by size: 812 391 390 AvaI C'yCGr_G Cuts at: 0 1158 1593 Size: 1158 435 BanI G'GyrC_C Cuts at: 0 169 1325 1593 Size: 169 1156 268 Fragments arranged by size: 1156 268 169 BbvCI CC'TCA_GC Cuts at: 0 1300 1593 Size: 1300 293 BciVI GTATCCnnnnn_n' Cuts at: 0 864 1479 1593 Size: 864 615 114 Fragments arranged by size: 864 615 114 BclI T'GATC_A Cuts at: 0 36 1593 Size: 36 1557 BfrBI ATG'CAT Cuts at: 0 123 165 453 1593 Size: 123 42 288 1140 Fragments arranged by size: 1140 288 123 42 BglI GCCn_nnn'nGGC Cuts at: 0 929 1593 Size: 929 664 Bme1580I G_kGCm'C Cuts at: 0 174 584 859 1532 1593 Size: 174 410 275 673 61 Fragments arranged by size: 673 410 275 174 61 BmrI ACTGGGnnnn_n' Cuts at: 0 421 844 1593 Size: 421 423 749 Fragments arranged by size: 749 423 421 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 170 272 861 1094 1527 1593 Size: 170 102 589 233 433 66 Fragments arranged by size: 589 433 233 170 102 66 Bpu10I CC'TnA_GC Cuts at: 0 1126 1300 1593 Size: 1126 174 293 Fragments arranged by size: 1126 293 174 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1192 1593 Size: 1192 401 BsaI GGTCTCn'nnnn_ Cuts at: 0 1093 1593 Size: 1093 500 BsaBI GATnn'nnATC Cuts at: 0 544 1593 Size: 544 1049 BsaHI Gr'CG_yC Cuts at: 0 755 1593 Size: 755 838 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 580 610 874 904 1073 1103 1288 1318 Size: 580 30 264 30 169 30 185 30 Cuts at: 1318 1593 Size: 275 Fragments arranged by size: 580 275 264 185 169 30 30 30 30 BsiHKAI G_wGCw'C Cuts at: 0 584 859 1593 Size: 584 275 734 Fragments arranged by size: 734 584 275 BsmI GAATG_Cn' Cuts at: 0 123 412 480 1130 1593 Size: 123 289 68 650 463 Fragments arranged by size: 650 463 289 123 68 Bsp1286I G_dGCh'C Cuts at: 0 174 584 859 1532 1593 Size: 174 410 275 673 61 Fragments arranged by size: 673 410 275 174 61 BsrDI GCAATG_nn' Cuts at: 0 167 1424 1593 Size: 167 1257 169 Fragments arranged by size: 1257 169 167 BstAPI GCAn_nnn'nTGC Cuts at: 0 1044 1169 1581 1593 Size: 1044 125 412 12 Fragments arranged by size: 1044 412 125 12 BstEII G'GTnAC_C Cuts at: 0 980 1593 Size: 980 613 BstYI r'GATC_y Cuts at: 0 1009 1593 Size: 1009 584 Bsu36I CC'TnA_GG Cuts at: 0 327 1145 1593 Size: 327 818 448 Fragments arranged by size: 818 448 327 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 468 1593 Size: 468 1125 BtsI GCAGTG_nn' Cuts at: 0 4 417 1593 Size: 4 413 1176 Fragments arranged by size: 1176 413 4 DraIII CAC_nnn'GTG Cuts at: 0 579 1593 Size: 579 1014 EaeI y'GGCC_r Cuts at: 0 623 930 1593 Size: 623 307 663 Fragments arranged by size: 663 623 307 EarI CTCTTCn'nnn_ Cuts at: 0 48 758 1593 Size: 48 710 835 Fragments arranged by size: 835 710 48 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 170 272 517 613 861 986 1094 1258 Size: 170 102 245 96 248 125 108 164 Cuts at: 1258 1527 1593 Size: 269 66 Fragments arranged by size: 269 248 245 170 164 125 108 102 96 66 EcoO109I rG'GnC_Cy Cuts at: 0 323 1212 1502 1593 Size: 323 889 290 91 Fragments arranged by size: 889 323 290 91 EcoRV GAT'ATC Cuts at: 0 542 666 1593 Size: 542 124 927 Fragments arranged by size: 927 542 124 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1017 1593 Size: 1017 576 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 580 612 1073 1105 1288 1320 1593 Size: 580 32 461 32 183 32 273 Fragments arranged by size: 580 461 273 183 32 32 32 HincII GTy'rAC Cuts at: 0 264 555 1359 1593 Size: 264 291 804 234 Fragments arranged by size: 804 291 264 234 HindIII A'AGCT_T Cuts at: 0 999 1593 Size: 999 594 HpaI GTT'AAC Cuts at: 0 264 1593 Size: 264 1329 KpnI G_GTAC'C Cuts at: 0 1329 1593 Size: 1329 264 MscI TGG'CCA Cuts at: 0 625 932 1593 Size: 625 307 661 Fragments arranged by size: 661 625 307 MslI CAynn'nnrTG Cuts at: 0 1525 1593 Size: 1525 68 NsiI A_TGCA'T Cuts at: 0 125 167 455 1593 Size: 125 42 288 1138 Fragments arranged by size: 1138 288 125 42 NspI r_CATG'y Cuts at: 0 123 315 453 457 1323 1593 Size: 123 192 138 4 866 270 Fragments arranged by size: 866 270 192 138 123 4 PciI A'CATG_T Cuts at: 0 1319 1593 Size: 1319 274 PfoI T'CCnGG_A Cuts at: 0 896 1593 Size: 896 697 PpuMI rG'GwC_Cy Cuts at: 0 323 1212 1502 1593 Size: 323 889 290 91 Fragments arranged by size: 889 323 290 91 PstI C_TGCA'G Cuts at: 0 792 1049 1593 Size: 792 257 544 Fragments arranged by size: 792 544 257 SanDI GG'GwC_CC Cuts at: 0 1502 1593 Size: 1502 91 SapI GCTCTTCn'nnn_ Cuts at: 0 48 1593 Size: 48 1545 SfcI C'TryA_G Cuts at: 0 776 788 1045 1593 Size: 776 12 257 548 Fragments arranged by size: 776 548 257 12 SmlI C'TyrA_G Cuts at: 0 1207 1593 Size: 1207 386 SphI G_CATG'C Cuts at: 0 123 457 1593 Size: 123 334 1136 Fragments arranged by size: 1136 334 123 StuI AGG'CCT Cuts at: 0 1114 1593 Size: 1114 479 StyI C'CwwG_G Cuts at: 0 1497 1593 Size: 1497 96 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 957 1593 Size: 957 636 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1494 1526 1593 Size: 1494 32 67 Fragments arranged by size: 1494 67 32 Tth111I GACn'n_nGTC Cuts at: 0 1085 1593 Size: 1085 508 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 465 1593 Size: 465 1128 ZraI GAC'GTC Cuts at: 0 756 1593 Size: 756 837 Enzymes that do cut: AatII Acc65I AcuI AflIII AleI AlwNI ApaLI ApoI AvaI BanI BbvCI BciVI BclI BfrBI BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BsiHKAI BsmI Bsp1286I BsrDI BstAPI BstEII BstYI Bsu36I BtgZI BtsI DraIII EaeI EarI Eco57MI EcoO109I EcoRV FalI Hin4I HincII HindIII HpaI KpnI MscI MslI NsiI NspI PciI PfoI PpuMI PstI SanDI SapI SfcI SmlI SphI StuI StyI TaqII TstI Tth111I XcmI ZraI Enzymes that do not cut: AarI AccI AclI AfeI AflII AgeI AhdI AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI BanII BbeI BbsI BcgI BglII BlpI BmgBI BmtI BplI BsaAI BsaWI BseRI BseYI BsgI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstXI BstZ17I BtgI ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoNI EcoRI FseI FspI FspAI HaeII KasI MfeI MluI MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TatI XbaI XhoI XmaI XmnI