################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BlnI BsaAI BsaBI BsaHI BsaI BseRI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI HphI KasI KpnI KspI MamI MfeI MluI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccIII 494 TCCGGA T^CCGGA AlwI 621 GGATC GGATC(4/5) AlwNI 763 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 424 GAGCTC G[AT]GC[AT]^C BalI 1079 TGGCCA TGG^CCA BanI 1121 GGTGCC G^G[CT][GA]CC BanII 424 GAGCTC G[GA]GC[CT]^C BbsI 447 GAAGAC GAAGAC(2/6) BclI 566 TGATCA T^GATCA BglII 46 AGATCT A^GATCT BlpI 426 GCTCAGC GC^T[ACGT]AGC BmyI 424 GAGCTC G[AGT]GC[ACT]^C BpmI 769 CTGGAG CTGGAG(16/14) Bpu1102I 426 GCTCAGC GC^T[ACGT]AGC BpuAI 447 GAAGAC GAAGAC(2/6) BsaWI 494 TCCGGA [AT]^CCGG[AT] BseAI 494 TCCGGA T^CCGGA BsiHKAI 424 GAGCTC G[AT]GC[AT]^C BsmAI 550 GAGAC GTCTC(1/5) Bsp1286I 424 GAGCTC G[AGT]GC[ACT]^C BspEI 494 TCCGGA T^CCGGA BspHI 531 TCATGA T^CATGA CelII 426 GCTCAGC GC^T[ACGT]AGC DraII 773 AGGACCT [GA]G^G[ACGT]CC[CT] EaeI 1079 TGGCCA [CT]^GGCC[GA] EcoNI 1077 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 773 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 1087 GAATTC G^AATTC EcoT14I 1082 CCAAGG C^C[AT][AT]GG EcoT22I 698 ATGCAT ATGCA^T FbaI 566 TGATCA T^GATCA HaeIII 1080 GGCC GG^CC HapII 495 CCGG C^CGG HincII 1299 GTCAAC GT[CT]^[GA]AC HindII 1299 GTCAAC GT[CT]^[GA]AC HpaII 495 CCGG C^CGG MaeII 187 ACGT A^CGT MaeIII 1037 GTGAC ^GT[ACGT]AC MluNI 1079 TGGCCA TGG^CCA MroI 494 TCCGGA T^CCGGA MscI 1079 TGGCCA TGG^CCA MslI 1003 CATTTGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 495 CCGG C^CGG MwoI 321 GCCCAATATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NsiI 698 ATGCAT ATGCA^T PflMI 762 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 773 AGGACCT [GA]G^G[AT]CC[CT] RcaI 531 TCATGA T^CATGA SacI 424 GAGCTC GAGCT^C SmlI 169 CTTGAG C^T[CT][GA]AG SspI 821 AATATT AAT^ATT StyI 1082 CCAAGG C^C[AT][AT]GG Tsp45I 1037 GTGAC ^GT[GC]AC Van91I 762 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 738 TCTAGA T^CTAGA XcmI 364 CCAGTTCCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AciI 378 GCGG CCGC(-3/-1) 904 GCGG AcsI 992 AAATTC [GA]^AATT[CT] 1087 GAATTC ApoI 992 AAATTC [GA]^AATT[CT] 1087 GAATTC Asp700I 259 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 486 GAAAACTTTC AvaII 579 GGACC G^G[AT]CC 774 GGACC BbvI 318 GCAGC GCAGC(8/12) 916 GCTGC BfaI 281 CTAG C^TAG 739 CTAG BsaJI 575 CCCAGG C^C[ACGT][ACGT]GG 1082 CCAAGG BsrDI 1043 CATTGC GCAATG(2/0) 1169 GCAATG BstYI 46 AGATCT [GA]^GATC[CT] 621 GGATCT Cfr13I 579 GGACC G^G[ACGT]CC 774 GGACC EarI 73 GAAGAG CTCTTC(1/4) 106 GAAGAG Fnu4HI 318 GCAGC GC^[ACGT]GC 916 GCTGC HindIII 112 AAGCTT A^AGCTT 292 AAGCTT ItaI 318 GCAGC GC^[ACGT]GC 916 GCTGC Ksp632I 73 GAAGAG CTCTTC(1/4) 106 GAAGAG MaeI 281 CTAG C^TAG 739 CTAG MflI 46 AGATCT [GA]^GATC[CT] 621 GGATCT MspA1I 383 CAGCTG C[AC]G^C[GT]G 802 CAGCTG PvuII 383 CAGCTG CAG^CTG 802 CAGCTG Sau96I 579 GGACC G^G[ACGT]CC 774 GGACC TaqI 537 TCGA T^CGA 663 TCGA TseI 318 GCAGC G^C[AT]GC 916 GCTGC TthHB8I 537 TCGA T^CGA 663 TCGA XhoII 46 AGATCT [GA]^GATC[CT] 621 GGATCT XmnI 259 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 486 GAAAACTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AluI BsiYI BslI BsrI BssKI BstF5I BstNI DdeI DpnII EcoRII FokI HinfI MboI MboII MnlI MseI MvaI NdeII NlaIII NlaIV PleI Sau3AI ScrFI SfaNI TfiI Tru9I Tsp509I TspRI