(Circular) (Six-base) MAPSORT of: pFN21AE3911.seq Check: 6937 from: 1 to: 6835 pFN21AE3911 6835 bp With 182 enzymes: SgfI * March 28, 2012 11:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4645 278 Size: 53 83 186 936 3109 2468 Fragments arranged by size: 3109 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3090 4178 1937 Size: 1153 1088 4594 Fragments arranged by size: 4594 1153 1088 Acc65I G'GTAC_C Cuts at: 4156 4156 Size: 6835 AclI AA'CG_TT Cuts at: 1563 4962 5335 1563 Size: 3399 373 3063 Fragments arranged by size: 3399 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2043 3305 3497 4898 6282 6550 1632 Size: 411 1262 192 1401 1384 268 1917 Fragments arranged by size: 1917 1401 1384 1262 411 268 192 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6638 AflIII A'CryG_T Cuts at: 1203 3045 3690 5734 6711 1203 Size: 1842 645 2044 977 1327 Fragments arranged by size: 2044 1842 1327 977 645 AgeI A'CCGG_T Cuts at: 5666 5666 Size: 6835 AhdI GACnn_n'nnGTC Cuts at: 5564 5564 Size: 6835 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6835 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2613 2807 3227 1184 Size: 1429 194 420 4792 Fragments arranged by size: 4792 1429 420 194 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2822 2854 2822 Size: 32 6803 AlwNI CAG_nnn'CTG Cuts at: 3682 4578 6150 3682 Size: 896 1572 4367 Fragments arranged by size: 4367 1572 896 ApaLI G'TGCA_C Cuts at: 3316 3863 4892 6048 3316 Size: 547 1029 1156 4103 Fragments arranged by size: 4103 1156 1029 547 ApoI r'AATT_y Cuts at: 1493 2188 3552 4144 4370 4522 6631 1493 Size: 695 1364 592 226 152 2109 1697 Fragments arranged by size: 2109 1697 1364 695 592 226 152 AseI AT'TA_AT Cuts at: 160 1032 5389 160 Size: 872 4357 1606 Fragments arranged by size: 4357 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6835 AvaI C'yCGr_G Cuts at: 1870 1943 2404 2423 2771 4160 1870 Size: 73 461 19 348 1389 4545 Fragments arranged by size: 4545 1389 461 348 73 19 BamHI G'GATC_C Cuts at: 4165 4532 4165 Size: 367 6468 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3445 4156 5611 618 Size: 325 217 627 1658 711 1455 1842 Fragments arranged by size: 1842 1658 1455 711 627 325 217 BanII G_rGCy'C Cuts at: 1391 2307 2377 2535 3192 3909 4154 1391 Size: 916 70 158 657 717 245 4072 Fragments arranged by size: 4072 916 717 657 245 158 70 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2616 2711 928 Size: 971 717 95 5052 Fragments arranged by size: 5052 971 717 95 BbvCI CC'TCA_GC Cuts at: 4083 4083 Size: 6835 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5026 5060 1903 Size: 34 3089 34 3678 Fragments arranged by size: 3678 3089 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4727 5937 4727 Size: 1210 5625 BclI T'GATC_A Cuts at: 1548 2059 3405 1548 Size: 511 1346 4978 Fragments arranged by size: 4978 1346 511 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5446 136 Size: 107 122 71 631 605 3774 1525 Fragments arranged by size: 3774 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2718 4014 6830 2718 Size: 1296 2816 2723 Fragments arranged by size: 2816 2723 1296 BlpI GC'TnA_GC Cuts at: 3879 4252 3879 Size: 373 6462 Bme1580I G_kGCm'C Cuts at: 1790 3320 3450 3867 4896 6052 1790 Size: 1530 130 417 1029 1156 2573 Fragments arranged by size: 2573 1530 1156 1029 417 130 BmgBI CAC'GTC Cuts at: 1318 2974 3046 1318 Size: 1656 72 5107 Fragments arranged by size: 5107 1656 72 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4566 5524 442 Size: 416 53 83 399 24 3149 958 1753 Fragments arranged by size: 3149 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6835 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2413 2724 2413 Size: 311 6524 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2347 2527 3023 3029 3131 3683 3731 3923 Size: 1108 180 496 6 102 552 48 192 Cuts at: 3923 5495 1239 Size: 1572 2579 Fragments arranged by size: 2579 1572 1108 552 496 192 180 102 48 6 Bpu10I CC'TnA_GC Cuts at: 4083 4083 Size: 6835 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2115 2728 2816 3245 4031 4960 5825 6123 2115 Size: 613 88 429 786 929 865 298 2827 Fragments arranged by size: 2827 929 865 786 613 429 298 88 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2003 5498 882 Size: 617 504 3495 2219 Fragments arranged by size: 3495 2219 617 504 BsaAI yAC'GTr Cuts at: 493 1204 3874 4626 493 Size: 711 2670 752 2702 Fragments arranged by size: 2702 2670 752 711 BsaBI GATnn'nnATC Cuts at: 6829 6829 Size: 6835 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2861 3375 3752 4642 Size: 53 83 186 936 1328 514 377 890 Cuts at: 4642 5024 5659 275 Size: 382 635 1451 Fragments arranged by size: 1451 1328 936 890 635 514 382 377 186 83 53 BsaWI w'CCGG_w Cuts at: 2778 3015 4032 5268 5666 5940 6087 6498 2778 Size: 237 1017 1236 398 274 147 411 3115 Fragments arranged by size: 3115 1236 1017 411 398 274 237 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2822 2852 1178 Size: 30 165 30 1419 30 5161 Fragments arranged by size: 5161 1419 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2135 2441 2666 2681 2684 2687 2690 2693 Size: 949 306 225 15 3 3 3 3 Cuts at: 2693 2828 3038 3211 3671 4025 1186 Size: 135 210 173 460 354 3996 Fragments arranged by size: 3996 949 460 354 306 225 210 173 135 15 3 3 3 3 BseYI C'CCAG_C Cuts at: 3995 4114 6038 6534 3995 Size: 119 1924 496 4296 Fragments arranged by size: 4296 1924 496 119 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2915 3223 3953 1163 Size: 573 1179 308 730 4045 Fragments arranged by size: 4045 1179 730 573 308 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4285 5046 6074 1540 Size: 275 186 2284 761 1028 2301 Fragments arranged by size: 2301 2284 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3015 3192 3320 3867 3909 4896 4981 6052 3015 Size: 177 128 547 42 987 85 1071 3798 Fragments arranged by size: 3798 1071 987 547 177 128 85 42 BsmI GAATG_Cn' Cuts at: 3411 4346 4439 3411 Size: 935 93 5807 Fragments arranged by size: 5807 935 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2307 2377 2535 3015 3192 3320 3450 Size: 399 517 70 158 480 177 128 130 Cuts at: 3450 3867 3909 4154 4896 4981 6052 1391 Size: 417 42 245 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 517 480 417 399 245 177 158 130 128 85 70 42 BspEI T'CCGG_A Cuts at: 2778 6498 2778 Size: 3720 3115 BspHI T'CATG_A Cuts at: 4724 6454 4724 Size: 1730 5105 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2892 3585 4190 844 Size: 2048 693 605 3489 Fragments arranged by size: 3489 2048 693 605 BsrBI CCG'CTC Cuts at: 1394 1823 2914 2992 3127 3310 4722 1394 Size: 429 1091 78 135 183 1412 3507 Fragments arranged by size: 3507 1412 1091 429 183 135 78 BsrDI GCAATG_nn' Cuts at: 65 1248 2164 2361 5330 5504 65 Size: 1183 916 197 2969 174 1396 Fragments arranged by size: 2969 1396 1183 916 197 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2434 2683 3591 5479 5666 1700 Size: 110 624 249 908 1888 187 2869 Fragments arranged by size: 2869 1888 908 624 249 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6835 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6835 BssSI C'ACGA_G Cuts at: 2888 4895 5907 2888 Size: 2007 1012 3816 Fragments arranged by size: 3816 2007 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6835 BstBI TT'CG_AA Cuts at: 5639 5639 Size: 6835 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6835 BstXI CCAn_nnnn'nTGG Cuts at: 2266 2968 2266 Size: 702 6133 BstYI r'GATC_y Cuts at: 1972 2718 2764 2775 2799 2925 3951 4014 4029 Size: 746 46 11 24 126 1026 63 15 Cuts at: 4029 4165 4532 4917 4934 6386 6472 6484 6830 Size: 136 367 385 17 1452 86 12 346 Cuts at: 6830 1972 Size: 1977 Fragments arranged by size: 1977 1452 1026 746 385 367 346 136 126 86 63 46 24 17 15 12 11 Bsu36I CC'TnA_GG Cuts at: 2811 3527 2811 Size: 716 6119 BtgI C'CryG_G Cuts at: 513 1065 2004 3635 513 Size: 552 939 1631 3713 Fragments arranged by size: 3713 1631 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6096 Fragments arranged by size: 6096 683 56 BtsI GCAGTG_nn' Cuts at: 3318 4351 5145 5165 3318 Size: 1033 794 20 4988 Fragments arranged by size: 4988 1033 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6835 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6800 DraI TTT'AAA Cuts at: 4139 4139 Size: 6835 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5842 809 Size: 507 4526 1802 Fragments arranged by size: 4526 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2432 2442 2631 Size: 54 1425 50 275 18 602 10 189 Cuts at: 2631 2685 3684 4282 5171 6800 8 Size: 54 999 598 889 1629 43 Fragments arranged by size: 1629 1425 999 889 602 598 275 189 54 54 50 43 18 10 EagI C'GGCC_G Cuts at: 1537 1812 4282 1537 Size: 275 2470 4090 Fragments arranged by size: 4090 2470 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2658 2960 3281 3291 3548 3566 4765 1353 Size: 1305 302 321 10 257 18 1199 3423 Fragments arranged by size: 3423 1305 1199 321 302 257 18 10 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5422 5796 5942 387 Size: 1064 138 3833 374 146 1280 Fragments arranged by size: 3833 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3190 3907 3190 Size: 717 6118 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2043 2347 2527 3023 3029 3131 3305 Size: 393 411 304 180 496 6 102 174 Cuts at: 3305 3497 3683 3731 3923 4898 5495 6282 6550 Size: 192 186 48 192 975 597 787 268 Cuts at: 6550 1239 Size: 1524 Fragments arranged by size: 1524 975 787 597 496 411 393 304 268 192 192 186 180 174 102 48 6 EcoNI CCTnn'n_nnAGG Cuts at: 2540 2759 2540 Size: 219 6616 EcoO109I rG'GnC_Cy Cuts at: 2470 2625 3506 2470 Size: 155 881 5799 Fragments arranged by size: 5799 881 155 EcoRI G'AATT_C Cuts at: 4144 4522 4144 Size: 378 6457 EcoRV GAT'ATC Cuts at: 2146 2146 Size: 6835 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3559 3559 Size: 6835 FseI GG_CCGG'CC Cuts at: 2438 2438 Size: 6835 FspI TGC'GCA Cuts at: 5341 5341 Size: 6835 HaeII r_GCGC'y Cuts at: 1678 5982 1678 Size: 4304 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3068 3100 4006 4038 5476 Size: 32 135 32 1496 32 906 32 1438 Cuts at: 5476 5508 5550 5582 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1496 1438 906 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4179 4425 669 Size: 988 281 2241 246 3079 Fragments arranged by size: 3079 2241 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6835 HpaI GTT'AAC Cuts at: 4425 4425 Size: 6835 KpnI G_GTAC'C Cuts at: 4160 4160 Size: 6835 MfeI C'AATT_G Cuts at: 4434 4434 Size: 6835 MluI A'CGCG_T Cuts at: 6711 6711 Size: 6835 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2985 3758 5949 6133 1305 Size: 1680 773 2191 184 2007 Fragments arranged by size: 2191 2007 1680 773 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3686 10 Size: 54 1425 343 1854 3159 Fragments arranged by size: 3159 1854 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3740 4793 5152 5311 518 Size: 648 2574 1053 359 159 2042 Fragments arranged by size: 2574 2042 1053 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2262 2787 3142 3468 3637 4251 4928 6076 Size: 656 525 355 326 169 614 677 1148 Cuts at: 6076 6321 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 656 614 525 355 326 245 169 NaeI GCC'GGC Cuts at: 2436 2685 3593 2436 Size: 249 908 5678 Fragments arranged by size: 5678 908 249 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5344 Fragments arranged by size: 5344 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6835 NgoMIV G'CCGG_C Cuts at: 2434 2683 3591 2434 Size: 249 908 5678 Fragments arranged by size: 5678 908 249 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6835 NotI GC'GGCC_GC Cuts at: 4282 4282 Size: 6835 NspI r_CATG'y Cuts at: 1132 1229 3366 3745 4193 5738 1132 Size: 97 2137 379 448 1545 2229 Fragments arranged by size: 2229 2137 1545 448 379 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6835 PciI A'CATG_T Cuts at: 5734 5734 Size: 6835 PflMI CCAn_nnn'nTGG Cuts at: 1108 2902 3682 4072 1108 Size: 1794 780 390 3871 Fragments arranged by size: 3871 1794 780 390 PfoI T'CCnGG_A Cuts at: 2504 2967 3000 3147 3165 3518 3601 3927 2504 Size: 463 33 147 18 353 83 326 5412 Fragments arranged by size: 5412 463 353 326 147 83 33 18 PmeI GTTT'AAAC Cuts at: 4139 4139 Size: 6835 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2822 2854 4904 4936 6417 6449 2822 Size: 32 2050 32 1481 32 3208 Fragments arranged by size: 3208 2050 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2625 2625 Size: 6835 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6835 PsiI TTA'TAA Cuts at: 4405 4405 Size: 6835 PstI C_TGCA'G Cuts at: 830 2829 3495 4187 830 Size: 1999 666 692 3478 Fragments arranged by size: 3478 1999 692 666 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6835 PvuII CAG'CTG Cuts at: 2262 3142 2262 Size: 880 5955 SacI G_AGCT'C Cuts at: 3192 3909 3192 Size: 717 6118 SacII CC_GC'GG Cuts at: 3638 3638 Size: 6835 SalI G'TCGA_C Cuts at: 1936 4177 1936 Size: 2241 4594 SanDI GG'GwC_CC Cuts at: 2625 2625 Size: 6835 SapI GCTCTTCn'nnn_ Cuts at: 3548 3548 Size: 6835 SbfI CC_TGCA'GG Cuts at: 4187 4187 Size: 6835 ScaI AGT'ACT Cuts at: 5083 6795 5083 Size: 1712 5123 SexAI A'CCwGG_T Cuts at: 3615 3615 Size: 6835 SfcI C'TryA_G Cuts at: 826 1045 2804 2825 3491 4183 5318 5999 6190 Size: 219 1759 21 666 692 1135 681 191 Cuts at: 6190 826 Size: 1471 Fragments arranged by size: 1759 1471 1135 692 681 666 219 191 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6835 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6835 SmaI CCC'GGG Cuts at: 1872 2406 4162 1872 Size: 534 1756 4545 Fragments arranged by size: 4545 1756 534 SmlI C'TyrA_G Cuts at: 820 1017 1943 2130 2743 2795 3260 4046 4939 Size: 197 926 187 613 52 465 786 893 Cuts at: 4939 5840 6102 820 Size: 901 262 1553 Fragments arranged by size: 1553 926 901 893 786 613 465 262 197 187 52 SnaBI TAC'GTA Cuts at: 493 4626 493 Size: 4133 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6835 SphI G_CATG'C Cuts at: 1132 3366 4193 1132 Size: 2234 827 3774 Fragments arranged by size: 3774 2234 827 SspI AAT'ATT Cuts at: 5 52 4759 5 Size: 47 4707 2081 Fragments arranged by size: 4707 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6835 StyI C'CwwG_G Cuts at: 513 1065 2004 2088 2229 4274 513 Size: 552 939 84 141 2045 3074 Fragments arranged by size: 3074 2045 939 552 141 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3882 4862 4879 5032 5217 5662 325 Size: 1255 2302 980 17 153 185 445 1498 Fragments arranged by size: 2302 1498 1255 980 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5081 6793 96 Size: 274 80 33 51 1443 3104 1712 138 Fragments arranged by size: 3104 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6795 6827 6795 Size: 32 6803 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6835 XbaI T'CTAG_A Cuts at: 4171 4171 Size: 6835 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2842 2842 Size: 6835 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6835 XmaI C'CCGG_G Cuts at: 1870 2404 4160 1870 Size: 534 1756 4545 Fragments arranged by size: 4545 1756 534 XmnI GAAnn'nnTTC Cuts at: 3542 4964 3542 Size: 1422 5413 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4643 276 Size: 53 83 186 936 3109 2468 Fragments arranged by size: 3109 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SfiI SfoI SrfI SwaI