################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaII AviII AvrII BanI BbeI BbvCI BcgI BciVI BcnI BfrI BglI BlnI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstZ17I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoRI EcoRV FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MspA1I MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PflFI PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AccII 779 CGCG CG^CG AcsI 788 AAATTT [GA]^AATT[CT] AflIII 361 ACGTGT A^C[GA][CT]GT Alw44I 104 GTGCAC G^TGCAC ApaLI 104 GTGCAC G^TGCAC ApoI 788 AAATTT [GA]^AATT[CT] Asp700I 65 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 104 GTGCAC G[AT]GC[AT]^C AvaI 1111 CTCGAG C^[CT]CG[GA]G BalI 1014 TGGCCA TGG^CCA BamHI 986 GGATCC G^GATCC BanII 1163 GGGCTC G[GA]GC[CT]^C BbrPI 360 CACGTG CAC^GTG BbsI 288 GAAGAC GAAGAC(2/6) BclI 498 TGATCA T^GATCA BfaI 1084 CTAG C^TAG BglII 165 AGATCT A^GATCT BpuAI 288 GAAGAC GAAGAC(2/6) BseRI 1054 CTCCTC GAGGAG(10/8) BsiHKAI 104 GTGCAC G[AT]GC[AT]^C BsmFI 957 GTCCC GGGAC(10/14) BsmI 883 GAATGC GAATGC(1/-1) BsoBI 1111 CTCGAG C^[CT]CG[GA]G BsrFI 701 ACCGGC [GA]^CCGG[CT] BstEII 1134 GGTTACC G^GT[ACGT]ACC BstPI 1134 GGTTACC G^GT[ACGT]ACC BstUI 779 CGCG CG^CG CfoI 1107 GCGC GCG^C Cfr10I 701 ACCGGC [GA]^CCGG[CT] Cfr13I 1071 GGCCC G^G[ACGT]CC EaeI 1014 TGGCCA [CT]^GGCC[GA] EcoO65I 1134 GGTTACC G^GT[ACGT]ACC EcoT14I 116 CCAAGG C^C[AT][AT]GG EcoT22I 885 ATGCAT ATGCA^T FbaI 498 TGATCA T^GATCA HaeII 1106 AGCGCC [GA]GCGC^[CT] HapII 702 CCGG C^CGG HgaI 823 GACGC GACGC(5/10) HhaI 1107 GCGC GCG^C HinP1I 1107 GCGC G^CGC HpaII 702 CCGG C^CGG MaeI 1084 CTAG C^TAG MluNI 1014 TGGCCA TGG^CCA MscI 1014 TGGCCA TGG^CCA MslI 1062 CACTGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 702 CCGG C^CGG MvnI 779 CGCG CG^CG NlaIV 986 GGATCC GG[ACGT]^[ACGT]CC NsiI 885 ATGCAT ATGCA^T PaeR7I 1111 CTCGAG C^TCGAG PmaCI 360 CACGTG CAC^GTG PmlI 360 CACGTG CAC^GTG Sau96I 1071 GGCCC G^G[ACGT]CC SfcI 706 CTACAG C^T[GA][CT]AG SmlI 1111 CTCGAG C^T[CT][GA]AG SspI 66 AATATT AAT^ATT StyI 116 CCAAGG C^C[AT][AT]GG XhoI 1111 CTCGAG C^TCGAG XmnI 65 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AocI 237 CCTGAGG CC^T[ACGT]AGG 564 CCTGAGG BlpI 246 GCTCAGC GC^T[ACGT]AGC 1165 GCTCAGC BmyI 104 GTGCAC G[AGT]GC[ACT]^C 1163 GGGCTC Bpu1102I 246 GCTCAGC GC^T[ACGT]AGC 1165 GCTCAGC BsaAI 360 CACGTG [CT]AC^GT[GA] 656 CACGTA Bsp1286I 104 GTGCAC G[AGT]GC[ACT]^C 1163 GGGCTC Bsu36I 237 CCTGAGG CC^T[ACGT]AGG 564 CCTGAGG CelII 246 GCTCAGC GC^T[ACGT]AGC 1165 GCTCAGC Eco81I 237 CCTGAGG CC^T[ACGT]AGG 564 CCTGAGG FauI 1029 GCGGG CCCGC(4/6) 1161 GCGGG HphI 699 TCACC GGTGA(8/7) 1208 TCACC MaeII 361 ACGT A^CGT 657 ACGT PflMI 116 CCAAGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 855 CCACGAATTGG Tsp45I 698 GTCAC ^GT[GC]AC 1207 GTCAC Van91I 116 CCAAGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 855 CCACGAATTGG ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI BbvI BpmI BsaJI BsgI BsiYI BslI BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII RsaI Sau3AI ScrFI SfaNI StuI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII