################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AatII Acc65I AclI AcyI AflII AgeI Alw44I ApaI ApaLI AscI Asp718I AspI AvaI AviII AvrII BalI BanI BbeI BbvCI BcgI BciVI BfrI BlnI BlpI Bpu1102I BsaBI BsaHI BsiEI BsiWI BsmBI BsoBI BspDI BsrBI BssHII Bst1107I BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI DraII DraIII DrdI EaeI EagI EclXI Eco52I EcoNI EcoO109I EcoO65I EcoT22I FauI FseI FspI Hin1I HincII HindII HpaI KasI KpnI KspI MamI MluI MluNI MscI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 1132 GTAGAC GT^[AC][GT]AC AccII 2453 CGCG CG^CG AccIII 2410 TCCGGA T^CCGGA AlwNI 3107 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 2100 CCTGAGG CC^T[ACGT]AGG Aor51HI 60 AGCGCT AGC^GCT AseI 2191 ATTAAT AT^TAAT AsnI 2191 ATTAAT AT^TAAT BamHI 2836 GGATCC G^GATCC BbrPI 1438 CACGTG CAC^GTG BclI 2400 TGATCA T^GATCA BglI 2330 GCCTGTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 3496 AGATCT A^GATCT BseAI 2410 TCCGGA T^CCGGA BsmI 2339 GCATTC GAATGC(1/-1) BspEI 2410 TCCGGA T^CCGGA BspHI 2727 TCATGA T^CATGA BsrFI 1569 ACCGGC [GA]^CCGG[CT] BssSI 3484 CTCGTG CACGAG(-5/-1) BstUI 2453 CGCG CG^CG Bsu36I 2100 CCTGAGG CC^T[ACGT]AGG Cfr10I 1569 ACCGGC [GA]^CCGG[CT] Eco47III 60 AGCGCT AGC^GCT Eco81I 2100 CCTGAGG CC^T[ACGT]AGG EcoRV 2434 GATATC GAT^ATC FbaI 2400 TGATCA T^GATCA HaeII 60 AGCGCT [GA]GCGC^[CT] HindIII 322 AAGCTT A^AGCTT MfeI 2527 CAATTG C^AATTG MroI 2410 TCCGGA T^CCGGA MunI 2527 CAATTG C^AATTG MvnI 2453 CGCG CG^CG NdeI 1652 CATATG CA^TATG PmaCI 1438 CACGTG CAC^GTG PmlI 1438 CACGTG CAC^GTG PshBI 2191 ATTAAT AT^TAAT RcaI 2727 TCATGA T^CATGA SacI 1178 GAGCTC GAGCT^C SspI 2498 AATATT AAT^ATT StuI 2801 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AflIII 744 ACATGT A^C[GA][CT]GT 3058 ACATGT AhdI 286 GACTTAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 958 GACGTGAAGTC AspEI 286 GACTTAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 958 GACGTGAAGTC BsaAI 1369 CACGTA [CT]AC^GT[GA] 1438 CACGTG BsaI 3268 GAGACC GGTCTC(1/5) 843 GGTCTC BsgI 1225 GTGCAG GTGCAG(16/14) 1514 GTGCAG Bsp1407I 1585 TGTACA T^GTACA 3336 TGTACA BspLU11I 744 ACATGT A^CATGT 3058 ACATGT BsrDI 2759 CATTGC GCAATG(2/0) 661 GCAATG BsrGI 1585 TGTACA T^GTACA 3336 TGTACA BstAPI 1120 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1330 GCAACACTTGC BstBI 203 TTCGAA TT^CGAA 410 TTCGAA Eam1105I 286 GACTTAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 958 GACGTGAAGTC NspV 203 TTCGAA TT^CGAA 410 TTCGAA PstI 1358 CTGCAG CTGCA^G 1877 CTGCAG PvuII 2789 CAGCTG CAG^CTG 3350 CAGCTG SfuI 203 TTCGAA TT^CGAA 410 TTCGAA SmlI 548 CTCAAG C^T[CT][GA]AG 1627 CTTGAG SspBI 1585 TGTACA T^GTACA 3336 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I AspHI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EarI EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI