Restriction map for
pFN21AE9666
(Circular) (Six-base) MAPSORT of: pFN21AE9666.seq Check: 8808 from: 1 to: 8263
pFN21AE9666 8263 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3136 3136
Size: 8263
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6073 278
Size: 53 83 186 936 4537 2468
Fragments arranged by size:
4537 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3138 5606 1937
Size: 1201 2468 4594
Fragments arranged by size:
4594 2468 1201
Acc65I G'GTAC_C
Cuts at: 5584 5584
Size: 8263
AclI AA'CG_TT
Cuts at: 1563 6390 6763 1563
Size: 4827 373 3063
Fragments arranged by size:
4827 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2356 2566 2619 2843 2874 3011 3474 3645
Size: 724 210 53 224 31 137 463 171
Cuts at: 3645 3750 3768 5367 6326 7710 7978 1632
Size: 105 18 1599 959 1384 268 1917
Fragments arranged by size:
1917 1599 1384 959 724 463 268 224
210 171 137 105 53 31 18
AfeI AGC'GCT
Cuts at: 2067 2067
Size: 8263
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8066
AflIII A'CryG_T
Cuts at: 1203 2749 5063 7162 8139 1203
Size: 1546 2314 2099 977 1327
Fragments arranged by size:
2314 2099 1546 1327 977
AgeI A'CCGG_T
Cuts at: 7094 7094
Size: 8263
AhdI GACnn_n'nnGTC
Cuts at: 2296 2968 6992 2296
Size: 672 4024 3567
Fragments arranged by size:
4024 3567 672
AleI CACnn'nnGTG
Cuts at: 1166 4742 1166
Size: 3576 4687
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4974 1184
Size: 3790 4473
AlwNI CAG_nnn'CTG
Cuts at: 5117 6006 7578 5117
Size: 889 1572 5802
Fragments arranged by size:
5802 1572 889
ApaLI G'TGCA_C
Cuts at: 6320 7476 6320
Size: 1156 7107
ApoI r'AATT_y
Cuts at: 1493 2211 3595 3668 3718 3841 4010 4246 4561
Size: 718 1384 73 50 123 169 236 315
Cuts at: 4561 4632 4643 4676 4883 5135 5572 5798 5950
Size: 71 11 33 207 252 437 226 152
Cuts at: 5950 8059 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1384 718 437 315 252 236
226 207 169 152 123 73 71 50
33 11
AseI AT'TA_AT
Cuts at: 160 1032 4197 6817 160
Size: 872 3165 2620 1606
Fragments arranged by size:
3165 2620 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8263
AvaI C'yCGr_G
Cuts at: 1870 1943 5588 1870
Size: 73 3645 4545
Fragments arranged by size:
4545 3645 73
BamHI G'GATC_C
Cuts at: 4841 5593 5960 4841
Size: 752 367 7144
Fragments arranged by size:
7144 752 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5584 7039 618
Size: 325 217 627 3797 1455 1842
Fragments arranged by size:
3797 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2022 3187 3405 3552 5582 1391
Size: 631 1165 218 147 2030 4072
Fragments arranged by size:
4072 2030 1165 631 218 147
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2862 2999 4925 928
Size: 971 963 137 1926 4266
Fragments arranged by size:
4266 1926 971 963 137
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6454 6488 1903
Size: 34 4517 34 3678
Fragments arranged by size:
4517 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6155 7365 6155
Size: 1210 7053
BclI T'GATC_A
Cuts at: 1548 4405 1548
Size: 2857 5406
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4341 6874 136
Size: 107 122 71 631 605 2669 2533 1525
Fragments arranged by size:
2669 2533 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5501 8258 5501
Size: 2757 5506
BlpI GC'TnA_GC
Cuts at: 5680 5680
Size: 8263
Bme1580I G_kGCm'C
Cuts at: 1790 6324 7480 1790
Size: 4534 1156 2573
Fragments arranged by size:
4534 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2965 4132 5537 1318
Size: 1647 1167 1405 4044
Fragments arranged by size:
4044 1647 1405 1167
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2394 5994 6952
Size: 416 53 83 399 24 977 3600 958
Cuts at: 6952 442
Size: 1753
Fragments arranged by size:
3600 1753 977 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8263
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 2293 3107 3533 4241 6923 1239
Size: 815 239 814 426 708 2682 2579
Fragments arranged by size:
2682 2579 815 814 708 426 239
Bpu10I CC'TnA_GC
Cuts at: 5072 5072
Size: 8263
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2538 3653 6388 7253 7551 2538
Size: 1115 2735 865 298 3250
Fragments arranged by size:
3250 2735 1115 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2854 5267 6926 882
Size: 617 1355 2413 1659 2219
Fragments arranged by size:
2413 2219 1659 1355 617
BsaAI yAC'GTr
Cuts at: 493 1204 3376 3445 6054 493
Size: 711 2172 69 2609 2702
Fragments arranged by size:
2702 2609 2172 711 69
BsaBI GATnn'nnATC
Cuts at: 8257 8257
Size: 8263
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6070 6452 7087 275
Size: 53 83 186 936 4537 382 635 1451
Fragments arranged by size:
4537 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4298 4415 4545 6696 7094 7368 7515 7926 4298
Size: 117 130 2151 398 274 147 411 4635
Fragments arranged by size:
4635 2151 411 398 274 147 130 117
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2819 2849 3131 3161 3534
Size: 30 165 30 1416 30 282 30 373
Cuts at: 3534 3564 1178
Size: 30 5877
Fragments arranged by size:
5877 1416 373 282 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2690 3095 3383 4220 1186
Size: 1504 405 288 837 5229
Fragments arranged by size:
5229 1504 837 405 288
BseYI C'CCAG_C
Cuts at: 3061 3218 4792 4962 7466 7962 3061
Size: 157 1574 170 2504 496 3362
Fragments arranged by size:
3362 2504 1574 496 170 157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3251 3540 1163
Size: 573 1515 289 5886
Fragments arranged by size:
5886 1515 573 289
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5713 6474 7502 1540
Size: 275 186 3712 761 1028 2301
Fragments arranged by size:
3712 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2042 3187 3375 6324 6409 7480 2042
Size: 1145 188 2949 85 1071 2825
Fragments arranged by size:
2949 2825 1145 1071 188 85
BsmI GAATG_Cn'
Cuts at: 4344 5774 5867 4344
Size: 1430 93 6740
Fragments arranged by size:
6740 1430 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2042 3187 3375 3405 3552 5582
Size: 399 232 20 1145 188 30 147 2030
Cuts at: 5582 6324 6409 7480 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 2030 1145 1071 742 399 232 188
147 85 30 20
BspEI T'CCGG_A
Cuts at: 4415 7926 4415
Size: 3511 4752
BspHI T'CATG_A
Cuts at: 4732 6152 7882 4732
Size: 1420 1730 5113
Fragments arranged by size:
5113 1730 1420
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2041 3136 5285 5618 844
Size: 1197 1095 2149 333 3489
Fragments arranged by size:
3489 2149 1197 1095 333
BsrBI CCG'CTC
Cuts at: 1394 1823 6150 1394
Size: 429 4327 3507
Fragments arranged by size:
4327 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2673 4763 6758 6932 65
Size: 1183 1425 2090 1995 174 1396
Fragments arranged by size:
2090 1995 1425 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3574 6907 7094 1700
Size: 110 1764 3333 187 2869
Fragments arranged by size:
3333 2869 1764 187 110
BsrGI T'GTAC_A
Cuts at: 96 3590 5341 96
Size: 3494 1751 3018
Fragments arranged by size:
3494 3018 1751
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8263
BssSI C'ACGA_G
Cuts at: 5489 6323 7335 5489
Size: 834 1012 6417
Fragments arranged by size:
6417 1012 834
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3131 3341 794
Size: 2337 210 5716
Fragments arranged by size:
5716 2337 210
BstBI TT'CG_AA
Cuts at: 2209 2416 7067 2209
Size: 207 4651 3405
Fragments arranged by size:
4651 3405 207
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8263
BstYI r'GATC_y
Cuts at: 1972 2079 4841 5300 5501 5593 5960 6345 6362
Size: 107 2762 459 201 92 367 385 17
Cuts at: 6362 7814 7900 7912 8258 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2762 1977 1452 459 385 367 346 201
107 92 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4106 4106
Size: 8263
BtgI C'CryG_G
Cuts at: 513 1065 2004 2140 3112 3915 4365 4626 4821
Size: 552 939 136 972 803 450 261 195
Cuts at: 4821 5124 513
Size: 303 3652
Fragments arranged by size:
3652 972 939 803 552 450 303 261
195 136
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7524
Fragments arranged by size:
7524 683 56
BtsI GCAGTG_nn'
Cuts at: 3528 4388 5779 6573 6593 3528
Size: 860 1391 794 20 5198
Fragments arranged by size:
5198 1391 860 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8263
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2826 2861 3897 3932 529
Size: 35 2262 35 1036 35 4860
Fragments arranged by size:
4860 2262 1036 35 35 35
DraI TTT'AAA
Cuts at: 5567 5567
Size: 8263
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7270 809
Size: 507 5954 1802
Fragments arranged by size:
5954 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5710 6599 8228
Size: 54 1425 50 275 18 3880 889 1629
Cuts at: 8228 8
Size: 43
Fragments arranged by size:
3880 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5710 1537
Size: 275 3898 4090
Fragments arranged by size:
4090 3898 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2267 2413 2472 2529 2931 3072 3687 4044
Size: 914 146 59 57 402 141 615 357
Cuts at: 4044 5168 5193 6193 1353
Size: 1124 25 1000 3423
Fragments arranged by size:
3423 1124 1000 914 615 402 357 146
141 59 57 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6850 7224 7370 387
Size: 1064 138 5261 374 146 1280
Fragments arranged by size:
5261 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3185 3185
Size: 8263
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2293 2356 2566 2619 2843 2874
Size: 393 422 239 63 210 53 224 31
Cuts at: 2874 3011 3107 3474 3533 3645 3750 3768 4241
Size: 137 96 367 59 112 105 18 473
Cuts at: 4241 5367 6326 6923 7710 7978 1239
Size: 1126 959 597 787 268 1524
Fragments arranged by size:
1524 1126 959 787 597 473 422 393
367 268 239 224 210 137 112 105
96 63 59 53 31 18
EcoRI G'AATT_C
Cuts at: 2211 3718 4010 4246 4643 5572 5950 2211
Size: 1507 292 236 397 929 378 4524
Fragments arranged by size:
4524 1507 929 397 378 292 236
EcoRV GAT'ATC
Cuts at: 4441 4441
Size: 8263
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2732 3798 4861 5346 2732
Size: 1066 1063 485 5649
Fragments arranged by size:
5649 1066 1063 485
FspI TGC'GCA
Cuts at: 6769 6769
Size: 8263
HaeII r_GCGC'y
Cuts at: 1678 2069 7410 1678
Size: 391 5341 2531
Fragments arranged by size:
5341 2531 391
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2282 2314 2343 2375 2555
Size: 32 135 32 710 32 29 32 180
Cuts at: 2555 2587 2819 2851 2906 2938 2954 2986 3082
Size: 32 232 32 55 32 16 32 96
Cuts at: 3082 3114 3131 3163 6904 6936 6978 7010 1373
Size: 32 17 32 3741 32 42 32 2626
Fragments arranged by size:
3741 2626 710 232 180 135 96 55
42 32 32 32 32 32 32 32
32 32 32 32 32 29 17 16
HincII GTy'rAC
Cuts at: 669 1657 1938 5607 5853 669
Size: 988 281 3669 246 3079
Fragments arranged by size:
3669 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2327 748
Size: 1579 6684
HpaI GTT'AAC
Cuts at: 5853 5853
Size: 8263
KpnI G_GTAC'C
Cuts at: 5588 5588
Size: 8263
MfeI C'AATT_G
Cuts at: 4532 5862 4532
Size: 1330 6933
MluI A'CGCG_T
Cuts at: 8139 8139
Size: 8263
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3608 4191 7377 7561 1305
Size: 2303 583 3186 184 2007
Fragments arranged by size:
3186 2303 2007 583 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6441
Fragments arranged by size:
6441 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2977 3971 4742 6221 6580 6739 518
Size: 648 1811 994 771 1479 359 159 2042
Fragments arranged by size:
2042 1811 1479 994 771 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4796 4894 5357 5679 6356 7504 7749 1606
Size: 3190 98 463 322 677 1148 245 2120
Fragments arranged by size:
3190 2120 1148 677 463 322 245 98
NcoI C'CATG_G
Cuts at: 513 1065 2004 4365 4626 5124 513
Size: 552 939 2361 261 498 3652
Fragments arranged by size:
3652 2361 939 552 498 261
NdeI CA'TA_TG
Cuts at: 387 3658 387
Size: 3271 4992
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8263
NotI GC'GGCC_GC
Cuts at: 5710 5710
Size: 8263
NspI r_CATG'y
Cuts at: 1132 1229 2753 2956 3623 4466 5067 5256 5621
Size: 97 1524 203 667 843 601 189 365
Cuts at: 5621 7166 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 1524 843 667 601 365 203
189 97
PasI CC'CwG_GG
Cuts at: 1348 4649 1348
Size: 3301 4962
PciI A'CATG_T
Cuts at: 2749 5063 7162 2749
Size: 2314 2099 3850
Fragments arranged by size:
3850 2314 2099
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8263
PfoI T'CCnGG_A
Cuts at: 2130 2309 4311 4392 2130
Size: 179 2002 81 6001
Fragments arranged by size:
6001 2002 179 81
PmeI GTTT'AAAC
Cuts at: 5567 5567
Size: 8263
PmlI CAC'GTG
Cuts at: 3445 3445
Size: 8263
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6332 6364 7845 7877 6332
Size: 32 1481 32 6718
Fragments arranged by size:
6718 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8263
PsiI TTA'TAA
Cuts at: 5833 5833
Size: 8263
PstI C_TGCA'G
Cuts at: 830 3367 3886 5615 830
Size: 2537 519 1729 3478
Fragments arranged by size:
3478 2537 1729 519
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8263
PvuII CAG'CTG
Cuts at: 4796 5357 4796
Size: 561 7702
SacI G_AGCT'C
Cuts at: 3187 3187
Size: 8263
SalI G'TCGA_C
Cuts at: 1936 5605 1936
Size: 3669 4594
SbfI CC_TGCA'GG
Cuts at: 5615 5615
Size: 8263
ScaI AGT'ACT
Cuts at: 6511 8223 6511
Size: 1712 6551
SfcI C'TryA_G
Cuts at: 826 1045 3135 3363 3882 4468 5611 6746 7427
Size: 219 2090 228 519 586 1143 1135 681
Cuts at: 7427 7618 826
Size: 191 1471
Fragments arranged by size:
2090 1471 1143 1135 681 586 519 228
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8263
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8263
SmaI CCC'GGG
Cuts at: 1872 5590 1872
Size: 3718 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2553 3632 6367 7268 7530 820
Size: 197 926 610 1079 2735 901 262 1553
Fragments arranged by size:
2735 1553 1079 926 901 610 262 197
SnaBI TAC'GTA
Cuts at: 493 6054 493
Size: 5561 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8263
SphI G_CATG'C
Cuts at: 1132 5621 1132
Size: 4489 3774
SspI AAT'ATT
Cuts at: 5 52 4505 6187 5
Size: 47 4453 1682 2081
Fragments arranged by size:
4453 2081 1682 47
StuI AGG'CCT
Cuts at: 1517 4808 1517
Size: 3291 4972
StyI C'CwwG_G
Cuts at: 513 1065 2004 4365 4626 5124 5702 513
Size: 552 939 2361 261 498 578 3074
Fragments arranged by size:
3074 2361 939 578 552 498 261
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6290 6307 6460 6645 7090 325
Size: 1255 4710 17 153 185 445 1498
Fragments arranged by size:
4710 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3590 5341 6509
Size: 274 80 33 51 1443 1613 1751 1168
Cuts at: 6509 8221 96
Size: 1712 138
Fragments arranged by size:
1751 1712 1613 1443 1168 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3288 3320 3534 3566 8223 8255 3288
Size: 32 214 32 4657 32 3296
Fragments arranged by size:
4657 3296 214 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8263
XbaI T'CTAG_A
Cuts at: 5599 5599
Size: 8263
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8263
XmaI C'CCGG_G
Cuts at: 1870 5588 1870
Size: 3718 4545
XmnI GAAnn'nnTTC
Cuts at: 2633 3459 4984 6392 2633
Size: 826 1525 1408 4504
Fragments arranged by size:
4504 1525 1408 826
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6071 276
Size: 53 83 186 936 4537 2468
Fragments arranged by size:
4537 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI