Restriction map for
pFN21AE9666
(Linear) (Six-base) MAPSORT of: KIEE9666.seq Check: 8979 from: 1 to: 3558
KIEE9666.orf
With 182 enzymes: SgfI *
December 27, 2011 16:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1131 3558
Size: 1131 2427
AccI GT'mk_AC
Cuts at: 0 1133 3558
Size: 1133 2425
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 561 614 838 869 1006 1469 1640
Size: 351 210 53 224 31 137 463 171
Cuts at: 1640 1745 1763 3362 3558
Size: 105 18 1599 196
Fragments arranged by size:
1599 463 351 224 210 196 171 137
105 53 31 18
AfeI AGC'GCT
Cuts at: 0 62 3558
Size: 62 3496
AflIII A'CryG_T
Cuts at: 0 744 3058 3558
Size: 744 2314 500
Fragments arranged by size:
2314 744 500
AhdI GACnn_n'nnGTC
Cuts at: 0 291 963 3558
Size: 291 672 2595
Fragments arranged by size:
2595 672 291
AleI CACnn'nnGTG
Cuts at: 0 2737 3558
Size: 2737 821
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2969 3558
Size: 2969 589
AlwNI CAG_nnn'CTG
Cuts at: 0 3112 3558
Size: 3112 446
ApoI r'AATT_y
Cuts at: 0 206 1590 1663 1713 1836 2005 2241 2556
Size: 206 1384 73 50 123 169 236 315
Cuts at: 2556 2627 2638 2671 2878 3130 3558
Size: 71 11 33 207 252 428
Fragments arranged by size:
1384 428 315 252 236 207 206 169
123 73 71 50 33 11
AseI AT'TA_AT
Cuts at: 0 2192 3558
Size: 2192 1366
BamHI G'GATC_C
Cuts at: 0 2836 3558
Size: 2836 722
BanII G_rGCy'C
Cuts at: 0 17 1182 1400 1547 3558
Size: 17 1165 218 147 2011
Fragments arranged by size:
2011 1165 218 147 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 857 994 2920 3558
Size: 857 137 1926 638
Fragments arranged by size:
1926 857 638 137
BclI T'GATC_A
Cuts at: 0 2400 3558
Size: 2400 1158
BglI GCCn_nnn'nGGC
Cuts at: 0 2336 3558
Size: 2336 1222
BglII A'GATC_T
Cuts at: 0 3496 3558
Size: 3496 62
BmgBI CAC'GTC
Cuts at: 0 960 2127 3532 3558
Size: 960 1167 1405 26
Fragments arranged by size:
1405 1167 960 26
BmrI ACTGGGnnnn_n'
Cuts at: 0 389 3558
Size: 389 3169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 288 1102 1528 2236 3558
Size: 49 239 814 426 708 1322
Fragments arranged by size:
1322 814 708 426 239 49
Bpu10I CC'TnA_GC
Cuts at: 0 3067 3558
Size: 3067 491
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 533 1648 3558
Size: 533 1115 1910
Fragments arranged by size:
1910 1115 533
BsaI GGTCTCn'nnnn_
Cuts at: 0 849 3262 3558
Size: 849 2413 296
Fragments arranged by size:
2413 849 296
BsaAI yAC'GTr
Cuts at: 0 1371 1440 3558
Size: 1371 69 2118
Fragments arranged by size:
2118 1371 69
BsaWI w'CCGG_w
Cuts at: 0 2293 2410 2540 3558
Size: 2293 117 130 1018
Fragments arranged by size:
2293 1018 130 117
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 814 844 1126 1156 1529 1559 3558
Size: 814 30 282 30 373 30 1999
Fragments arranged by size:
1999 814 373 282 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 685 1090 1378 2215 3558
Size: 685 405 288 837 1343
Fragments arranged by size:
1343 837 685 405 288
BseYI C'CCAG_C
Cuts at: 0 1056 1213 2787 2957 3558
Size: 1056 157 1574 170 601
Fragments arranged by size:
1574 1056 601 170 157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1246 1535 3558
Size: 1246 289 2023
Fragments arranged by size:
2023 1246 289
BsiHKAI G_wGCw'C
Cuts at: 0 37 1182 1370 3558
Size: 37 1145 188 2188
Fragments arranged by size:
2188 1145 188 37
BsmI GAATG_Cn'
Cuts at: 0 2339 3558
Size: 2339 1219
Bsp1286I G_dGCh'C
Cuts at: 0 17 37 1182 1370 1400 1547 3558
Size: 17 20 1145 188 30 147 2011
Fragments arranged by size:
2011 1145 188 147 30 20 17
BspEI T'CCGG_A
Cuts at: 0 2410 3558
Size: 2410 1148
BspHI T'CATG_A
Cuts at: 0 2727 3558
Size: 2727 831
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 36 1131 3280 3558
Size: 36 1095 2149 278
Fragments arranged by size:
2149 1095 278 36
BsrDI GCAATG_nn'
Cuts at: 0 668 2758 3558
Size: 668 2090 800
Fragments arranged by size:
2090 800 668
BsrFI r'CCGG_y
Cuts at: 0 1569 3558
Size: 1569 1989
BsrGI T'GTAC_A
Cuts at: 0 1585 3336 3558
Size: 1585 1751 222
Fragments arranged by size:
1751 1585 222
BssSI C'ACGA_G
Cuts at: 0 3484 3558
Size: 3484 74
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1126 1336 3558
Size: 1126 210 2222
Fragments arranged by size:
2222 1126 210
BstBI TT'CG_AA
Cuts at: 0 204 411 3558
Size: 204 207 3147
Fragments arranged by size:
3147 207 204
BstYI r'GATC_y
Cuts at: 0 74 2836 3295 3496 3558
Size: 74 2762 459 201 62
Fragments arranged by size:
2762 459 201 74 62
Bsu36I CC'TnA_GG
Cuts at: 0 2101 3558
Size: 2101 1457
BtgI C'CryG_G
Cuts at: 0 135 1107 1910 2360 2621 2816 3119 3558
Size: 135 972 803 450 261 195 303 439
Fragments arranged by size:
972 803 450 439 303 261 195 135
BtsI GCAGTG_nn'
Cuts at: 0 1523 2383 3558
Size: 1523 860 1175
Fragments arranged by size:
1523 1175 860
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 821 856 1892 1927 3558
Size: 821 35 1036 35 1631
Fragments arranged by size:
1631 1036 821 35 35
EarI CTCTTCn'nnn_
Cuts at: 0 262 408 467 524 926 1067 1682 2039
Size: 262 146 59 57 402 141 615 357
Cuts at: 2039 3163 3188 3558
Size: 1124 25 370
Fragments arranged by size:
1124 615 402 370 357 262 146 141
59 57 25
EcoICRI GAG'CTC
Cuts at: 0 1180 3558
Size: 1180 2378
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 288 351 561 614 838 869 1006
Size: 49 239 63 210 53 224 31 137
Cuts at: 1006 1102 1469 1528 1640 1745 1763 2236 3362
Size: 96 367 59 112 105 18 473 1126
Cuts at: 3362 3558
Size: 196
Fragments arranged by size:
1126 473 367 239 224 210 196 137
112 105 96 63 59 53 49 31
18
EcoRI G'AATT_C
Cuts at: 0 206 1713 2005 2241 2638 3558
Size: 206 1507 292 236 397 920
Fragments arranged by size:
1507 920 397 292 236 206
EcoRV GAT'ATC
Cuts at: 0 2436 3558
Size: 2436 1122
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 727 1793 2856 3341 3558
Size: 727 1066 1063 485 217
Fragments arranged by size:
1066 1063 727 485 217
HaeII r_GCGC'y
Cuts at: 0 64 3558
Size: 64 3494
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 277 309 338 370 550 582 814 846
Size: 277 32 29 32 180 32 232 32
Cuts at: 846 901 933 949 981 1077 1109 1126 1158
Size: 55 32 16 32 96 32 17 32
Cuts at: 1158 3558
Size: 2400
Fragments arranged by size:
2400 277 232 180 96 55 32 32
32 32 32 32 32 32 29 17
16
HindIII A'AGCT_T
Cuts at: 0 322 3558
Size: 322 3236
MfeI C'AATT_G
Cuts at: 0 2527 3558
Size: 2527 1031
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1603 2186 3558
Size: 1603 583 1372
Fragments arranged by size:
1603 1372 583
MslI CAynn'nnrTG
Cuts at: 0 972 1966 2737 3558
Size: 972 994 771 821
Fragments arranged by size:
994 972 821 771
MspA1I CmG'CkG
Cuts at: 0 2791 2889 3352 3558
Size: 2791 98 463 206
Fragments arranged by size:
2791 463 206 98
NcoI C'CATG_G
Cuts at: 0 2360 2621 3119 3558
Size: 2360 261 498 439
Fragments arranged by size:
2360 498 439 261
NdeI CA'TA_TG
Cuts at: 0 1653 3558
Size: 1653 1905
NspI r_CATG'y
Cuts at: 0 748 951 1618 2461 3062 3251 3558
Size: 748 203 667 843 601 189 307
Fragments arranged by size:
843 748 667 601 307 203 189
PasI CC'CwG_GG
Cuts at: 0 2644 3558
Size: 2644 914
PciI A'CATG_T
Cuts at: 0 744 3058 3558
Size: 744 2314 500
Fragments arranged by size:
2314 744 500
PfoI T'CCnGG_A
Cuts at: 0 125 304 2306 2387 3558
Size: 125 179 2002 81 1171
Fragments arranged by size:
2002 1171 179 125 81
PmlI CAC'GTG
Cuts at: 0 1440 3558
Size: 1440 2118
PstI C_TGCA'G
Cuts at: 0 1362 1881 3558
Size: 1362 519 1677
Fragments arranged by size:
1677 1362 519
PvuII CAG'CTG
Cuts at: 0 2791 3352 3558
Size: 2791 561 206
Fragments arranged by size:
2791 561 206
SacI G_AGCT'C
Cuts at: 0 1182 3558
Size: 1182 2376
SfcI C'TryA_G
Cuts at: 0 1130 1358 1877 2463 3558
Size: 1130 228 519 586 1095
Fragments arranged by size:
1130 1095 586 519 228
SmlI C'TyrA_G
Cuts at: 0 548 1627 3558
Size: 548 1079 1931
Fragments arranged by size:
1931 1079 548
SspI AAT'ATT
Cuts at: 0 2500 3558
Size: 2500 1058
StuI AGG'CCT
Cuts at: 0 2803 3558
Size: 2803 755
StyI C'CwwG_G
Cuts at: 0 2360 2621 3119 3558
Size: 2360 261 498 439
Fragments arranged by size:
2360 498 439 261
TatI w'GTAC_w
Cuts at: 0 1585 3336 3558
Size: 1585 1751 222
Fragments arranged by size:
1751 1585 222
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1283 1315 1529 1561 3558
Size: 1283 32 214 32 1997
Fragments arranged by size:
1997 1283 214 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 628 1454 2979 3558
Size: 628 826 1525 579
Fragments arranged by size:
1525 826 628 579
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AhdI AleI AlfI
AlwNI ApoI AseI BamHI BanII BbsI BclI BglI
BglII BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtsI CspCI EarI EcoICRI
Eco57MI EcoRI EcoRV FalI HaeII Hin4I HindIII MfeI
MmeI MslI MspA1I NcoI NdeI NspI PasI PciI
PfoI PmlI PstI PvuII SacI SfcI SmlI SspI
StuI StyI TatI TstI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AloI ApaI ApaLI
AscI AsiSI AvaI AvrII BaeI BanI BbeI BbvCI
BcgI BciVI BfrBI BlpI Bme1580I BmtI BplI BsaBI
BsaHI BsiEI BsiWI BsmBI BsrBI BssHII BstEII BstXI
BstZ17I BtgZI ClaI DraI DraIII DrdI EaeI EagI
EciI EcoNI EcoO109I FseI FspI FspAI HincII HpaI
KasI KpnI MluI MscI NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI
TaqII Tth111I XbaI XcmI XhoI XmaI ZraI