################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## Acc65I AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvaI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII SacI SacII SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 461 GACGTC GACGT^C AccI 458 GTCGAC GT^[AC][GT]AC AcyI 461 GACGTC G[GA]^CG[CT]C AseI 327 ATTAAT AT^TAAT AsnI 327 ATTAAT AT^TAAT AspHI 675 GTGCTC G[AT]GC[AT]^C AvaI 559 CCCGAG C^[CT]CG[GA]G BcgI 460 CGACGTCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 600 CCGGG CC^[GC]GG BmyI 675 GTGCTC G[AGT]GC[ACT]^C BpmI 490 CTGGAG CTGGAG(16/14) BsaHI 461 GACGTC G[GA]^CG[CT]C BsaI 187 GAGACC GGTCTC(1/5) BsaWI 635 ACCGGA [AT]^CCGG[AT] BseRI 289 GAGGAG GAGGAG(10/8) BsiHKAI 675 GTGCTC G[AT]GC[AT]^C BsmBI 5 GAGACG CGTCTC(1/5) BsoBI 559 CCCGAG C^[CT]CG[GA]G Bsp1286I 675 GTGCTC G[AGT]GC[ACT]^C BspHI 614 TCATGA T^CATGA BsrBI 143 CCGCTC CCGCTC(-3/-3) BssKI 600 CCGGG ^CC[ACGT]GG BstYI 346 AGATCC [GA]^GATC[CT] CpoI 424 CGGTCCG CG^G[AT]CCG DsaI 594 CCGTGG C^C[GA][CT]GG HgaI 7 GACGC GACGC(5/10) Hin1I 461 GACGTC G[GA]^CG[CT]C HincII 458 GTCGAC GT[CT]^[GA]AC HindII 458 GTCGAC GT[CT]^[GA]AC MaeII 462 ACGT A^CGT MflI 346 AGATCC [GA]^GATC[CT] MseI 328 TTAA T^TAA MslI 615 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NciI 600 CCGGG CC^[GC]GG NlaIV 70 GGGTCC GG[ACGT]^[ACGT]CC PleI 456 GAGTC GAGTC(4/5) PshBI 327 ATTAAT AT^TAAT RcaI 614 TCATGA T^CATGA RsrII 424 CGGTCCG CG^G[AT]CCG SalI 458 GTCGAC G^TCGAC ScrFI 600 CCGGG CC^[ACGT]GG SfaNI 662 GCATC GCATC(5/9) SmlI 409 CTTGAG C^T[CT][GA]AG Tru9I 328 TTAA T^TAA Tsp45I 197 GTGAC ^GT[GC]AC Tsp509I 509 AATT ^AATT XhoII 346 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AfaI 152 GTAC GT^AC 356 GTAC AlwI 347 GATCC GGATC(4/5) 514 GGATC BsaJI 428 CCGAGG C^C[ACGT][ACGT]GG 594 CCGTGG BsgI 573 CTGCAC GTGCAG(16/14) 499 GTGCAG BsiYI 594 CCGTGGCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 636 CCGGAAAGTGG BslI 594 CCGTGGCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 636 CCGGAAAGTGG BsmFI 53 GTCCC GGGAC(10/14) 236 GTCCC DdeI 232 CTTAG C^T[ACGT]AG 371 CTGAG EaeI 397 CGGCCA [CT]^GGCC[GA] 597 TGGCCG EarI 184 GAAGAG CTCTTC(1/4) 453 GAAGAG HaeII 30 AGCGCC [GA]GCGC^[CT] 647 GGCGCT HapII 600 CCGG C^CGG 636 CCGG HpaII 600 CCGG C^CGG 636 CCGG Ksp632I 184 GAAGAG CTCTTC(1/4) 453 GAAGAG MboII 184 GAAGA GAAGA(8/7) 453 GAAGA MspI 600 CCGG C^CGG 636 CCGG RsaI 152 GTAC GT^AC 356 GTAC TspRI 195 CAGTG CA[GC]TG[ACGT][ACGT]^ 475 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AciI AluI AvaII BbvI BsmAI BsrI Cac8I CfoI Cfr13I DpnII Fnu4HI HaeIII HhaI HinP1I HinfI HphI ItaI MaeIII MboI MnlI MwoI NdeII NlaIII Sau3AI Sau96I TaqI TfiI TseI TthHB8I XcmI