(Circular) (Six-base) MAPSORT of: pFN21AB6233.seq Check: 6987 from: 1 to: 6949 pFN21AB6233 6949 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2044 3475 2044 Size: 1431 5518 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4759 278 Size: 53 83 186 936 3223 2468 Fragments arranged by size: 3223 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4292 1937 Size: 2355 4594 Acc65I G'GTAC_C Cuts at: 4270 4270 Size: 6949 AclI AA'CG_TT Cuts at: 1563 3293 5076 5449 1563 Size: 1730 1783 373 3063 Fragments arranged by size: 3063 1783 1730 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2258 2391 2762 3294 3998 4266 5012 6396 Size: 626 133 371 532 704 268 746 1384 Cuts at: 6396 6664 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 746 704 626 532 371 268 268 133 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6752 AflIII A'CryG_T Cuts at: 1203 3417 5848 6825 1203 Size: 2214 2431 977 1327 Fragments arranged by size: 2431 2214 1327 977 AgeI A'CCGG_T Cuts at: 5780 5780 Size: 6949 AhdI GACnn_n'nnGTC Cuts at: 5678 5678 Size: 6949 AleI CACnn'nnGTG Cuts at: 1166 3836 1166 Size: 2670 4279 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6949 AlwNI CAG_nnn'CTG Cuts at: 4692 6264 4692 Size: 1572 5377 ApaLI G'TGCA_C Cuts at: 5006 6162 5006 Size: 1156 5793 ApoI r'AATT_y Cuts at: 1493 2091 2282 2513 3197 4258 4484 4636 6745 Size: 598 191 231 684 1061 226 152 2109 Cuts at: 6745 1493 Size: 1697 Fragments arranged by size: 2109 1697 1061 684 598 231 226 191 152 AseI AT'TA_AT Cuts at: 160 1032 5503 160 Size: 872 4471 1606 Fragments arranged by size: 4471 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6949 AvaI C'yCGr_G Cuts at: 1870 1943 4274 1870 Size: 73 2331 4545 Fragments arranged by size: 4545 2331 73 BamHI G'GATC_C Cuts at: 4279 4646 4279 Size: 367 6582 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3782 4270 5725 618 Size: 325 217 627 1995 488 1455 1842 Fragments arranged by size: 1995 1842 1455 627 488 325 217 BanII G_rGCy'C Cuts at: 1391 2769 4268 1391 Size: 1378 1499 4072 Fragments arranged by size: 4072 1499 1378 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5978 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5140 5174 1903 Size: 34 3203 34 3678 Fragments arranged by size: 3678 3203 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4841 6051 4841 Size: 1210 5739 BclI T'GATC_A Cuts at: 1548 1548 Size: 6949 BfrBI ATG'CAT Cuts at: 2302 2302 Size: 6949 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3318 5560 136 Size: 107 122 71 631 605 1646 2242 1525 Fragments arranged by size: 2242 1646 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2500 3306 6944 2500 Size: 806 3638 2505 Fragments arranged by size: 3638 2505 806 BlpI GC'TnA_GC Cuts at: 4366 4366 Size: 6949 Bme1580I G_kGCm'C Cuts at: 1790 5010 6166 1790 Size: 3220 1156 2573 Fragments arranged by size: 3220 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6949 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2140 4680 5638 Size: 416 53 83 399 24 723 2540 958 Cuts at: 5638 442 Size: 1753 Fragments arranged by size: 2540 1753 958 723 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6949 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3361 3361 Size: 6949 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3258 3509 5609 1239 Size: 2019 251 2100 2579 Fragments arranged by size: 2579 2100 2019 251 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5074 5939 6237 5074 Size: 865 298 5786 Fragments arranged by size: 5786 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3332 5612 882 Size: 617 1833 2280 2219 Fragments arranged by size: 2280 2219 1833 617 BsaAI yAC'GTr Cuts at: 493 1204 4740 493 Size: 711 3536 2702 Fragments arranged by size: 3536 2702 711 BsaBI GATnn'nnATC Cuts at: 6943 6943 Size: 6949 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4756 5138 5773 275 Size: 53 83 186 936 3223 382 635 1451 Fragments arranged by size: 3223 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5382 5780 6054 6201 6612 5382 Size: 398 274 147 411 5719 Fragments arranged by size: 5719 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6724 Fragments arranged by size: 6724 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2356 2858 1186 Size: 1170 502 5277 Fragments arranged by size: 5277 1170 502 BseYI C'CCAG_C Cuts at: 6152 6648 6152 Size: 496 6453 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2486 1163 Size: 573 750 5626 Fragments arranged by size: 5626 750 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4399 5160 6188 1540 Size: 275 186 2398 761 1028 2301 Fragments arranged by size: 2398 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2439 5010 5095 6166 2439 Size: 2571 85 1071 3222 Fragments arranged by size: 3222 2571 1071 85 BsiWI C'GTAC_G Cuts at: 3516 3516 Size: 6949 BsmI GAATG_Cn' Cuts at: 4460 4553 4460 Size: 93 6856 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2439 2769 4268 5010 5095 6166 1391 Size: 399 649 330 1499 742 85 1071 2174 Fragments arranged by size: 2174 1499 1071 742 649 399 330 85 BspEI T'CCGG_A Cuts at: 6612 6612 Size: 6949 BspHI T'CATG_A Cuts at: 4838 6568 4838 Size: 1730 5219 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2044 3475 4304 844 Size: 1200 1431 829 3489 Fragments arranged by size: 3489 1431 1200 829 BsrBI CCG'CTC Cuts at: 1394 1823 4836 1394 Size: 429 3013 3507 Fragments arranged by size: 3507 3013 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3347 4230 5444 5618 65 Size: 1183 2099 883 1214 174 1396 Fragments arranged by size: 2099 1396 1214 1183 883 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3897 5593 5780 1700 Size: 110 2087 1696 187 2869 Fragments arranged by size: 2869 2087 1696 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6949 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6949 BssSI C'ACGA_G Cuts at: 5009 6021 5009 Size: 1012 5937 BstAPI GCAn_nnn'nTGC Cuts at: 794 2605 794 Size: 1811 5138 BstBI TT'CG_AA Cuts at: 5753 5753 Size: 6949 BstEII G'GTnAC_C Cuts at: 1184 3596 1184 Size: 2412 4537 BstXI CCAn_nnnn'nTGG Cuts at: 3334 3334 Size: 6949 BstYI r'GATC_y Cuts at: 1972 2500 3306 3679 4279 4646 5031 5048 6500 Size: 528 806 373 600 367 385 17 1452 Cuts at: 6500 6586 6598 6944 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 806 600 528 385 373 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2085 2157 2571 513 Size: 552 1020 72 414 4891 Fragments arranged by size: 4891 1020 552 414 72 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6210 Fragments arranged by size: 6210 683 56 BtsI GCAGTG_nn' Cuts at: 2594 4465 5259 5279 2594 Size: 1871 794 20 4264 Fragments arranged by size: 4264 1871 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6949 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4195 4230 529 Size: 35 3631 35 3248 Fragments arranged by size: 3631 3248 35 35 DraI TTT'AAA Cuts at: 4253 4253 Size: 6949 DraIII CAC_nnn'GTG Cuts at: 2443 3268 2443 Size: 825 6124 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5956 809 Size: 507 4640 1802 Fragments arranged by size: 4640 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4396 5285 6914 Size: 54 1425 50 275 18 2566 889 1629 Cuts at: 6914 8 Size: 43 Fragments arranged by size: 2566 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4396 1537 Size: 275 2584 4090 Fragments arranged by size: 4090 2584 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2331 2697 4879 1353 Size: 978 366 2182 3423 Fragments arranged by size: 3423 2182 978 366 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5536 5910 6056 387 Size: 1064 138 3947 374 146 1280 Fragments arranged by size: 3947 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2258 2391 2762 3258 3294 3509 3998 Size: 393 626 133 371 496 36 215 489 Cuts at: 3998 4266 5012 5609 6396 6664 1239 Size: 268 746 597 787 268 1524 Fragments arranged by size: 1524 787 746 626 597 496 489 393 371 268 268 215 133 36 EcoO109I rG'GnC_Cy Cuts at: 2269 2378 2269 Size: 109 6840 EcoRI G'AATT_C Cuts at: 4258 4636 4258 Size: 378 6571 FspI TGC'GCA Cuts at: 5455 5455 Size: 6949 HaeII r_GCGC'y Cuts at: 1678 6096 1678 Size: 4418 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2107 2139 2233 2265 2533 Size: 32 135 32 535 32 94 32 268 Cuts at: 2533 2565 2834 2866 5590 5622 5664 5696 1373 Size: 32 269 32 2724 32 42 32 2626 Fragments arranged by size: 2724 2626 535 269 268 135 94 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3423 3482 4293 4539 669 Size: 988 281 1485 59 811 246 3079 Fragments arranged by size: 3079 1485 988 811 281 246 59 HindIII A'AGCT_T Cuts at: 748 3566 748 Size: 2818 4131 HpaI GTT'AAC Cuts at: 4539 4539 Size: 6949 KpnI G_GTAC'C Cuts at: 4274 4274 Size: 6949 MfeI C'AATT_G Cuts at: 2828 3090 4212 4548 2828 Size: 262 1122 336 5229 Fragments arranged by size: 5229 1122 336 262 MluI A'CGCG_T Cuts at: 6825 6825 Size: 6949 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2050 2839 6063 6247 1305 Size: 745 789 3224 184 2007 Fragments arranged by size: 3224 2007 789 745 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5127 Fragments arranged by size: 5127 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2885 3836 4907 5266 5425 518 Size: 648 1719 951 1071 359 159 2042 Fragments arranged by size: 2042 1719 1071 951 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4365 5042 6190 6435 1606 Size: 2759 677 1148 245 2120 Fragments arranged by size: 2759 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2085 2157 2571 513 Size: 552 1020 72 414 4891 Fragments arranged by size: 4891 1020 552 414 72 NdeI CA'TA_TG Cuts at: 387 2866 3552 387 Size: 2479 686 3784 Fragments arranged by size: 3784 2479 686 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6949 NotI GC'GGCC_GC Cuts at: 4396 4396 Size: 6949 NsiI A_TGCA'T Cuts at: 2304 2304 Size: 6949 NspI r_CATG'y Cuts at: 1132 1229 2306 2368 2905 4307 5852 1132 Size: 97 1077 62 537 1402 1545 2229 Fragments arranged by size: 2229 1545 1402 1077 537 97 62 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6949 PciI A'CATG_T Cuts at: 5848 5848 Size: 6949 PflMI CCAn_nnn'nTGG Cuts at: 1108 3408 3442 3556 1108 Size: 2300 34 114 4501 Fragments arranged by size: 4501 2300 114 34 PmeI GTTT'AAAC Cuts at: 4253 4253 Size: 6949 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5018 5050 6531 6563 5018 Size: 32 1481 32 5404 Fragments arranged by size: 5404 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2269 2269 Size: 6949 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6949 PsiI TTA'TAA Cuts at: 4519 4519 Size: 6949 PstI C_TGCA'G Cuts at: 830 2359 2601 4301 830 Size: 1529 242 1700 3478 Fragments arranged by size: 3478 1700 1529 242 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6949 SalI G'TCGA_C Cuts at: 1936 4291 1936 Size: 2355 4594 SapI GCTCTTCn'nnn_ Cuts at: 2697 2697 Size: 6949 SbfI CC_TGCA'GG Cuts at: 4301 4301 Size: 6949 ScaI AGT'ACT Cuts at: 5197 6909 5197 Size: 1712 5237 SexAI A'CCwGG_T Cuts at: 3634 3634 Size: 6949 SfcI C'TryA_G Cuts at: 826 1045 2355 2521 2597 3586 3876 4027 4297 Size: 219 1310 166 76 989 290 151 270 Cuts at: 4297 5432 6113 6304 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1310 1135 989 681 290 270 219 191 166 151 76 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6949 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6949 SmaI CCC'GGG Cuts at: 1872 4276 1872 Size: 2404 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5053 5954 6216 820 Size: 197 926 3110 901 262 1553 Fragments arranged by size: 3110 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4740 493 Size: 4247 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6949 SphI G_CATG'C Cuts at: 1132 4307 1132 Size: 3175 3774 SspI AAT'ATT Cuts at: 5 52 2349 3675 4873 5 Size: 47 2297 1326 1198 2081 Fragments arranged by size: 2297 2081 1326 1198 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6949 StyI C'CwwG_G Cuts at: 513 1065 2085 2157 2571 4388 513 Size: 552 1020 72 414 1817 3074 Fragments arranged by size: 3074 1817 1020 552 414 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3826 4976 4993 5146 5331 5776 325 Size: 1255 2246 1150 17 153 185 445 1498 Fragments arranged by size: 2246 1498 1255 1150 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3130 5195 6907 Size: 274 80 33 51 1443 1153 2065 1712 Cuts at: 6907 96 Size: 138 Fragments arranged by size: 2065 1712 1443 1153 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6909 6941 6909 Size: 32 6917 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6949 XbaI T'CTAG_A Cuts at: 2383 4285 2383 Size: 1902 5047 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3851 3851 Size: 6949 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6949 XmaI C'CCGG_G Cuts at: 1870 4274 1870 Size: 2404 4545 XmnI GAAnn'nnTTC Cuts at: 3279 3960 4123 5078 3279 Size: 681 163 955 5150 Fragments arranged by size: 5150 955 681 163 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4757 276 Size: 53 83 186 936 3223 2468 Fragments arranged by size: 3223 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI