################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BfrI BglI BlnI BsaHI BseRI BsgI BsiEI BsiWI BspDI BsrBI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstZ17I Bsu36I ClaI CpoI DraII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoT22I FseI FspI HgaI Hin1I HindIII KasI KpnI KspI MfeI MluI MunI MvnI NarI NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI RsrII SacII SalI SbfI ScaI SfcI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I StuI SwaI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccIII 803 TCCGGA T^CCGGA AseI 1362 ATTAAT AT^TAAT AsnI 1362 ATTAAT AT^TAAT Asp700I 1998 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 14 CCCGAG C^[CT]CG[GA]G BalI 1241 TGGCCA TGG^CCA BciVI 1958 GTATCC GTATCC(6/5) BclI 2309 TGATCA T^GATCA BglII 91 AGATCT A^GATCT BpmI 2012 CTGGAG CTGGAG(16/14) BsaAI 2282 CACGTA [CT]AC^GT[GA] BsaI 1294 GAGACC GGTCTC(1/5) BsaWI 803 TCCGGA [AT]^CCGG[AT] BseAI 803 TCCGGA T^CCGGA BsoBI 14 CCCGAG C^[CT]CG[GA]G Bsp1407I 2238 TGTACA T^GTACA BspEI 803 TCCGGA T^CCGGA BspLU11I 276 ACATGT A^CATGT BspMI 1528 GCAGGT ACCTGC(4/8) BsrFI 1226 GCCGGC [GA]^CCGG[CT] BsrGI 2238 TGTACA T^GTACA BstBI 1418 TTCGAA TT^CGAA Cfr10I 1226 GCCGGC [GA]^CCGG[CT] DraIII 1938 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1705 GACTGCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1535 CCATGG C^C[GA][CT]GG EcoRI 823 GAATTC G^AATTC FbaI 2309 TGATCA T^GATCA HaeII 1235 AGCGCC [GA]GCGC^[CT] HpaI 1924 GTTAAC GTT^AAC MluNI 1241 TGGCCA TGG^CCA MroI 803 TCCGGA T^CCGGA MscI 1241 TGGCCA TGG^CCA NaeI 1226 GCCGGC GCC^GGC NcoI 1535 CCATGG C^CATGG NgoMIV 1226 GCCGGC G^CCGGC NspV 1418 TTCGAA TT^CGAA PshBI 1362 ATTAAT AT^TAAT SapI 8 GAAGAGC GCTCTTC(1/4) SfuI 1418 TTCGAA TT^CGAA SphI 2204 GCATGC GCATG^C SspBI 2238 TGTACA T^GTACA XbaI 406 TCTAGA T^CTAGA XcmI 260 CCAGAGAAAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1998 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AcsI 701 AAATTC [GA]^AATT[CT] 823 GAATTC AlwNI 1234 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG 2128 CAGCTGCTG ApoI 701 AAATTC [GA]^AATT[CT] 823 GAATTC BanI 475 GGTGCC G^G[CT][GA]CC 991 GGCACC BbsI 1665 GAAGAC GAAGAC(2/6) 613 GTCTTC BcnI 2328 CCGGG CC^[GC]GG 2440 CCGGG BlpI 1108 GCTCAGC GC^T[ACGT]AGC 2133 GCTGAGC Bpu1102I 1108 GCTCAGC GC^T[ACGT]AGC 2133 GCTGAGC BpuAI 1665 GAAGAC GAAGAC(2/6) 613 GTCTTC BsaBI 558 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1764 GATGAGTATC BsmBI 1210 CGTCTC CGTCTC(1/5) 811 GAGACG BsrDI 83 CATTGC GCAATG(2/0) 128 GCAATG BstXI 53 CCACAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1244 CCAGGCTTGTGG CelII 1108 GCTCAGC GC^T[ACGT]AGC 2133 GCTGAGC CfoI 66 GCGC GCG^C 1236 GCGC DraI 1434 TTTAAA TTT^AAA 1839 TTTAAA EcoRV 177 GATATC GAT^ATC 1323 GATATC EcoT14I 1409 CCAAGG C^C[AT][AT]GG 1535 CCATGG HhaI 66 GCGC GCG^C 1236 GCGC HinP1I 66 GCGC G^CGC 1236 GCGC HincII 1382 GTCAAC GT[CT]^[GA]AC 1924 GTTAAC HindII 1382 GTCAAC GT[CT]^[GA]AC 1924 GTTAAC MamI 558 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1764 GATGAGTATC NciI 2328 CCGGG CC^[GC]GG 2440 CCGGG NdeI 1377 CATATG CA^TATG 2392 CATATG NspI 276 ACATGT [GA]CATG^[CT] 2204 GCATGC SacI 1132 GAGCTC GAGCT^C 1698 GAGCTC SexAI 1456 ACCTGGT A^CC[AT]GGT 1939 ACCAGGT SmlI 1515 CTTGAG C^T[CT][GA]AG 2169 CTCAAG SspI 1359 AATATT AAT^ATT 1691 AATATT StyI 1409 CCAAGG C^C[AT][AT]GG 1535 CCATGG TaqI 470 TCGA T^CGA 1419 TCGA TthHB8I 470 TCGA T^CGA 1419 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AciI AfaI AflIII AluI AlwI AspHI AvaII BanII BbvI BfaI BmyI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EaeI EarI EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII