(Circular) (Six-base) MAPSORT of: pFN21AE2266.seq Check: 2383 from: 1 to: 7261 pFN21AE2266 7261 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5071 278 Size: 53 83 186 936 3535 2468 Fragments arranged by size: 3535 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4604 1937 Size: 2667 4594 Acc65I G'GTAC_C Cuts at: 4582 4582 Size: 7261 AclI AA'CG_TT Cuts at: 1563 5388 5761 1563 Size: 3825 373 3063 Fragments arranged by size: 3825 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2597 2617 2676 3210 3339 3953 5324 6708 Size: 965 20 59 534 129 614 1371 1384 Cuts at: 6708 6976 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1371 965 614 534 268 129 59 20 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7064 AflIII A'CryG_T Cuts at: 1203 2281 2879 3212 4286 6160 7137 1203 Size: 1078 598 333 1074 1874 977 1327 Fragments arranged by size: 1874 1327 1078 1074 977 598 333 AgeI A'CCGG_T Cuts at: 6092 6092 Size: 7261 AhdI GACnn_n'nnGTC Cuts at: 5990 5990 Size: 7261 AleI CACnn'nnGTG Cuts at: 1166 2063 3011 4268 1166 Size: 897 948 1257 4159 Fragments arranged by size: 4159 1257 948 897 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7261 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4330 4362 4330 Size: 32 7229 AlwNI CAG_nnn'CTG Cuts at: 3244 4138 5004 6576 3244 Size: 894 866 1572 3929 Fragments arranged by size: 3929 1572 894 866 ApaLI G'TGCA_C Cuts at: 5318 6474 5318 Size: 1156 6105 ApoI r'AATT_y Cuts at: 1493 2706 2828 4570 4796 4948 7057 1493 Size: 1213 122 1742 226 152 2109 1697 Fragments arranged by size: 2109 1742 1697 1213 226 152 122 AseI AT'TA_AT Cuts at: 160 1032 3368 5815 160 Size: 872 2336 2447 1606 Fragments arranged by size: 2447 2336 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7261 AvaI C'yCGr_G Cuts at: 1870 1943 2019 4586 1870 Size: 73 76 2567 4545 Fragments arranged by size: 4545 2567 76 73 BamHI G'GATC_C Cuts at: 4591 4958 4591 Size: 367 6894 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2480 2996 4582 6037 618 Size: 325 217 627 693 516 1586 1455 1842 Fragments arranged by size: 1842 1586 1455 693 627 516 325 217 BanII G_rGCy'C Cuts at: 1391 2020 2904 3141 3707 4580 1391 Size: 629 884 237 566 873 4072 Fragments arranged by size: 4072 884 873 629 566 237 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2611 3677 928 Size: 971 712 1066 4512 Fragments arranged by size: 4512 1066 971 712 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5452 5486 1903 Size: 34 3515 34 3678 Fragments arranged by size: 3678 3515 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3974 5153 6363 3974 Size: 1179 1210 4872 Fragments arranged by size: 4872 1210 1179 BclI T'GATC_A Cuts at: 1548 4314 1548 Size: 2766 4495 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5872 136 Size: 107 122 71 631 605 4200 1525 Fragments arranged by size: 4200 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2096 7256 2096 Size: 5160 2101 BlpI GC'TnA_GC Cuts at: 3114 4139 4678 3114 Size: 1025 539 5697 Fragments arranged by size: 5697 1025 539 Bme1580I G_kGCm'C Cuts at: 1790 5322 6478 1790 Size: 3532 1156 2573 Fragments arranged by size: 3532 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2880 3215 3901 1318 Size: 1562 335 686 4678 Fragments arranged by size: 4678 1562 686 335 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2046 3710 4521 Size: 416 53 83 399 24 629 1664 811 Cuts at: 4521 4992 5950 442 Size: 471 958 1753 Fragments arranged by size: 1753 1664 958 811 629 471 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7261 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4038 5921 1239 Size: 2799 1883 2579 Fragments arranged by size: 2799 2579 1883 Bpu10I CC'TnA_GC Cuts at: 3135 3135 Size: 7261 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3541 4159 5386 6251 6549 3541 Size: 618 1227 865 298 4253 Fragments arranged by size: 4253 1227 865 618 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3293 5924 882 Size: 617 1794 2631 2219 Fragments arranged by size: 2631 2219 1794 617 BsaAI yAC'GTr Cuts at: 493 1204 4289 5052 493 Size: 711 3085 763 2702 Fragments arranged by size: 3085 2702 763 711 BsaBI GATnn'nnATC Cuts at: 2567 3773 7255 2567 Size: 1206 3482 2573 Fragments arranged by size: 3482 2573 1206 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5068 5450 6085 275 Size: 53 83 186 936 3535 382 635 1451 Fragments arranged by size: 3535 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2808 5694 6092 6366 6513 6924 2808 Size: 2886 398 274 147 411 3145 Fragments arranged by size: 3145 2886 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7036 Fragments arranged by size: 7036 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7261 BseYI C'CCAG_C Cuts at: 2712 4462 6464 6960 2712 Size: 1750 2002 496 3013 Fragments arranged by size: 3013 2002 1750 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6688 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4711 5472 6500 1540 Size: 275 186 2710 761 1028 2301 Fragments arranged by size: 2710 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3141 3707 4024 5322 5407 6478 3141 Size: 566 317 1298 85 1071 3924 Fragments arranged by size: 3924 1298 1071 566 317 85 BsmI GAATG_Cn' Cuts at: 2471 3651 4512 4772 4865 2471 Size: 1180 861 260 93 4867 Fragments arranged by size: 4867 1180 861 260 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2810 3221 2810 Size: 411 6850 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2020 2904 3141 3707 4024 4580 5322 Size: 399 230 884 237 566 317 556 742 Cuts at: 5322 5407 6478 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 884 742 566 556 399 317 237 230 85 BspEI T'CCGG_A Cuts at: 2808 6924 2808 Size: 4116 3145 BspHI T'CATG_A Cuts at: 2832 4011 4048 5150 6880 2832 Size: 1179 37 1102 1730 3213 Fragments arranged by size: 3213 1730 1179 1102 37 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3524 4616 844 Size: 2680 1092 3489 Fragments arranged by size: 3489 2680 1092 BsrBI CCG'CTC Cuts at: 1394 1823 5148 1394 Size: 429 3325 3507 Fragments arranged by size: 3507 3325 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2087 2140 5756 5930 65 Size: 1183 839 53 3616 174 1396 Fragments arranged by size: 3616 1396 1183 839 174 53 BsrFI r'CCGG_y Cuts at: 1700 1810 3231 5905 6092 1700 Size: 110 1421 2674 187 2869 Fragments arranged by size: 2869 2674 1421 187 110 BsrGI T'GTAC_A Cuts at: 96 4243 96 Size: 4147 3114 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7261 BssSI C'ACGA_G Cuts at: 5321 6333 5321 Size: 1012 6249 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7261 BstBI TT'CG_AA Cuts at: 3424 6065 3424 Size: 2641 4620 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7261 BstXI CCAn_nnnn'nTGG Cuts at: 2065 3256 2065 Size: 1191 6070 BstYI r'GATC_y Cuts at: 1972 2096 2157 2640 4591 4958 5343 5360 6812 Size: 124 61 483 1951 367 385 17 1452 Cuts at: 6812 6898 6910 7256 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1951 1452 483 385 367 346 124 86 61 17 12 BtgI C'CryG_G Cuts at: 513 1065 3540 513 Size: 552 2475 4234 Fragments arranged by size: 4234 2475 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2023 487 Size: 683 56 797 5725 Fragments arranged by size: 5725 797 683 56 BtsI GCAGTG_nn' Cuts at: 4150 4777 5571 5591 4150 Size: 627 794 20 5820 Fragments arranged by size: 5820 794 627 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7261 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7226 DraI TTT'AAA Cuts at: 3441 3846 4565 3441 Size: 405 719 6137 Fragments arranged by size: 6137 719 405 DraIII CAC_nnn'GTG Cuts at: 3948 3948 Size: 7261 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3716 6268 809 Size: 507 2400 2552 1802 Fragments arranged by size: 2552 2400 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3246 3340 4442 Size: 54 1425 50 275 18 1416 94 1102 Cuts at: 4442 4708 5597 7226 8 Size: 266 889 1629 43 Fragments arranged by size: 1629 1425 1416 1102 889 275 266 94 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4708 1537 Size: 275 2896 4090 Fragments arranged by size: 4090 2896 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2008 2573 3086 3520 4556 5191 1353 Size: 655 565 513 434 1036 635 3423 Fragments arranged by size: 3423 1036 655 635 565 513 434 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5848 6222 6368 387 Size: 1064 138 4259 374 146 1280 Fragments arranged by size: 4259 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3139 3705 3139 Size: 566 6695 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2597 2617 2676 3210 3339 3953 4038 Size: 393 965 20 59 534 129 614 85 Cuts at: 4038 5324 5921 6708 6976 1239 Size: 1286 597 787 268 1524 Fragments arranged by size: 1524 1286 965 787 614 597 534 393 268 129 85 59 20 EcoRI G'AATT_C Cuts at: 2828 4570 4948 2828 Size: 1742 378 5141 Fragments arranged by size: 5141 1742 378 EcoRV GAT'ATC Cuts at: 2184 3330 2184 Size: 1146 6115 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4510 4510 Size: 7261 FspI TGC'GCA Cuts at: 5767 5767 Size: 7261 HaeII r_GCGC'y Cuts at: 1678 3244 6408 1678 Size: 1566 3164 2531 Fragments arranged by size: 3164 2531 1566 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2393 2425 2576 2608 5902 Size: 32 135 32 821 32 151 32 3294 Cuts at: 5902 5934 5976 6008 1373 Size: 32 42 32 2626 Fragments arranged by size: 3294 2626 821 151 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3389 3931 4605 4851 669 Size: 988 281 1451 542 674 246 3079 Fragments arranged by size: 3079 1451 988 674 542 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7261 HpaI GTT'AAC Cuts at: 3931 4851 3931 Size: 920 6341 KpnI G_GTAC'C Cuts at: 4586 4586 Size: 7261 MfeI C'AATT_G Cuts at: 4860 4860 Size: 7261 MluI A'CGCG_T Cuts at: 7137 7137 Size: 7261 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2481 3892 6375 6559 1305 Size: 1176 1411 2483 184 2007 Fragments arranged by size: 2483 2007 1411 1176 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3248 10 Size: 54 1425 343 1416 4023 Fragments arranged by size: 4023 1425 1416 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2063 2750 3011 3767 4268 5219 5578 Size: 648 897 687 261 756 501 951 359 Cuts at: 5578 5737 518 Size: 159 2042 Fragments arranged by size: 2042 951 897 756 687 648 501 359 261 159 MspA1I CmG'CkG Cuts at: 1606 3187 3593 3640 3960 4102 4135 4677 5354 Size: 1581 406 47 320 142 33 542 677 Cuts at: 5354 6502 6747 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1581 1148 677 542 406 320 245 142 47 33 NaeI GCC'GGC Cuts at: 3233 3233 Size: 7261 NcoI C'CATG_G Cuts at: 513 1065 3540 513 Size: 552 2475 4234 Fragments arranged by size: 4234 2475 552 NdeI CA'TA_TG Cuts at: 387 3383 4398 387 Size: 2996 1015 3250 Fragments arranged by size: 3250 2996 1015 NgoMIV G'CCGG_C Cuts at: 3231 3231 Size: 7261 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7261 NotI GC'GGCC_GC Cuts at: 4708 4708 Size: 7261 NspI r_CATG'y Cuts at: 1132 1229 2285 4213 4619 6164 1132 Size: 97 1056 1928 406 1545 2229 Fragments arranged by size: 2229 1928 1545 1056 406 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7261 PciI A'CATG_T Cuts at: 2281 6160 2281 Size: 3879 3382 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7261 PmeI GTTT'AAAC Cuts at: 4565 4565 Size: 7261 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5330 5362 6843 6875 5330 Size: 32 1481 32 5716 Fragments arranged by size: 5716 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7261 PsiI TTA'TAA Cuts at: 4831 4831 Size: 7261 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2287 2319 2287 Size: 32 7229 PstI C_TGCA'G Cuts at: 830 4613 830 Size: 3783 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7261 PvuII CAG'CTG Cuts at: 3187 3593 3640 4102 4135 3187 Size: 406 47 462 33 6313 Fragments arranged by size: 6313 462 406 47 33 SacI G_AGCT'C Cuts at: 3141 3707 3141 Size: 566 6695 SalI G'TCGA_C Cuts at: 1936 4603 1936 Size: 2667 4594 SapI GCTCTTCn'nnn_ Cuts at: 2008 2008 Size: 7261 SbfI CC_TGCA'GG Cuts at: 4613 4613 Size: 7261 ScaI AGT'ACT Cuts at: 5509 7221 5509 Size: 1712 5549 SexAI A'CCwGG_T Cuts at: 3461 3944 4557 3461 Size: 483 613 6165 Fragments arranged by size: 6165 613 483 SfcI C'TryA_G Cuts at: 826 1045 4609 5744 6425 6616 826 Size: 219 3564 1135 681 191 1471 Fragments arranged by size: 3564 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7261 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7261 SmaI CCC'GGG Cuts at: 1872 4588 1872 Size: 2716 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3520 4174 5365 6266 6528 820 Size: 197 926 1577 654 1191 901 262 1553 Fragments arranged by size: 1577 1553 1191 926 901 654 262 197 SnaBI TAC'GTA Cuts at: 493 5052 493 Size: 4559 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7261 SphI G_CATG'C Cuts at: 1132 4213 4619 1132 Size: 3081 406 3774 Fragments arranged by size: 3774 3081 406 SspI AAT'ATT Cuts at: 5 52 3366 3698 5185 5 Size: 47 3314 332 1487 2081 Fragments arranged by size: 3314 2081 1487 332 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7261 StyI C'CwwG_G Cuts at: 513 1065 3414 3540 4700 513 Size: 552 2349 126 1160 3074 Fragments arranged by size: 3074 2349 1160 552 126 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2888 5288 5305 5458 5643 6088 325 Size: 1255 1308 2400 17 153 185 445 1498 Fragments arranged by size: 2400 1498 1308 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3577 4041 4243 Size: 274 80 33 51 1443 1600 464 202 Cuts at: 4243 4431 5507 7219 96 Size: 188 1076 1712 138 Fragments arranged by size: 1712 1600 1443 1076 464 274 202 188 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2304 2336 2708 2740 3537 3569 7221 7253 2304 Size: 32 372 32 797 32 3652 32 2312 Fragments arranged by size: 3652 2312 797 372 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7261 XbaI T'CTAG_A Cuts at: 2411 4597 2411 Size: 2186 5075 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2272 2272 Size: 7261 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7261 XmaI C'CCGG_G Cuts at: 1870 4586 1870 Size: 2716 4545 XmnI GAAnn'nnTTC Cuts at: 4007 5390 4007 Size: 1383 5878 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5069 276 Size: 53 83 186 936 3535 2468 Fragments arranged by size: 3535 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoNI EcoO109I FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SfiI SfoI SrfI SwaI